Optimized Production of Fc Fusion Proteins by Sortase Enzymatic Ligation

Enhancing the Pharmacokinetics and Antitumor Activity of an α-Amanitin-Based Small-Molecule Drug Conjugate via Conjugation with an Fc Domain

(2021) Francesca Gallo et al.   JOURNAL OF MEDICINAL CHEMISTRY

Efficient Sortase-Mediated Ligation Using a Common C-Terminal Fusion Tag

(2020) Sierra A. Reed et al.   BIOCONJUGATE CHEMISTRY

Manipulating the click reactivity of dibenzoazacyclooctynes: from an azide clicker to a caged acylation reagent

(2020) Wei Shi et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

An Off-the-Shelf Approach for the Production of Fc Fusion Proteins by Protein Trans-Splicing towards Generating a Lectibody In Vitro

(2020) Anniina Jaakkonen et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Therapeutic Fc‐fusion proteins: Current analytical strategies

(2020) Bastiaan L. Duivelshof et al.   JOURNAL OF SEPARATION SCIENCE

Facile and Efficient Chemoenzymatic Semisynthesis of Fc-Fusion Compounds for Half-Life Extension of Pharmaceutical Components

(2019) Shigeo Hirasawa et al.   BIOCONJUGATE CHEMISTRY

Construction of an immunotoxin via site-specific conjugation of anti-Her2 IgG and engineered Pseudomonas exotoxin A

(2019) Byeong Sung Lee et al.   Journal of Biological Engineering

Highly Efficient Mono-Functionalization of Knob-in-Hole Antibodies with Strain-Promoted Click Chemistry

(2019) Jorick J. Bruins et al.   ACS Omega

Monocyclic phenolic compounds stabilize human insulin and suppress its amorphous aggregation: In vitro and in vivo study

(2019) Sepideh Haghighi-Poodeh et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Dual Site-Specific Labeling of an Antibody Fragment through Sortase A and π-Clamp Conjugation

(2019) Michael D. Lee et al.   BIOCONJUGATE CHEMISTRY

Multivariate optimization of the refolding process of an incorrectly folded Fc-fusion protein in cell culture broth

(2019) Hossein Behrouz et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Quantification of Bioorthogonal Stability in Immune Phagocytes Using Flow Cytometry Reveals Rapid Degradation of Strained Alkynes

(2018) Thomas Bakkum et al.   ACS Chemical Biology

Clonal variation in productivity and proteolytic clipping of an Fc-fusion protein in CHO cells: Proteomic analysis suggests a role for defective protein folding and the UPR

(2018) Michael Henry et al.   JOURNAL OF BIOTECHNOLOGY

Functional interrogation and mining of natively paired human VH:VL antibody repertoires

(2018) Bo Wang et al.   NATURE BIOTECHNOLOGY

One-Pot Dual Labeling of IgG 1 and Preparation of C-to-C Fusion Proteins Through a Combination of Sortase A and Butelase 1

(2018) Thibault J. Harmand et al.   BIOCONJUGATE CHEMISTRY

A Versatile Chemo-Enzymatic Conjugation Approach Yields Homogeneous and Highly Potent Antibody-Drug Conjugates

(2017) Ying Xu et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Sortase A-Generated Highly Potent Anti-CD20-MMAE Conjugates for Efficient Elimination of B-Lineage Lymphomas

(2016) Liqiang Pan et al.   Small

Improved variants of SrtA for site-specific conjugation on antibodies and proteins with high efficiency

(2016) Long Chen et al.   Scientific Reports

Fusion Proteins for Half-Life Extension of Biologics as a Strategy to Make Biobetters

(2015) William R. Strohl   BIODRUGS

Site-specific albumination of a therapeutic protein with multi-subunit to prolong activity in vivo

(2015) Sung In Lim et al.   JOURNAL OF CONTROLLED RELEASE

Pharmacokinetic properties of IgG and various Fc fusion proteins in mice

(2015) Felix Unverdorben et al.   mAbs

Accurate secondary structure prediction and fold recognition for circular dichroism spectroscopy

(2015) András Micsonai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Generation of new peptide-Fc fusion proteins that mediate antibody-dependent cellular cytotoxicity against different types of cancer cells

(2015) Mouldy Sioud et al.   Molecular Therapy-Methods & Clinical Development

Sortase Enzyme-Mediated Generation of Site-Specifically Conjugated Antibody Drug Conjugates with High In Vitro and In Vivo Potency

(2015) Roger R. Beerli et al.   PLoS One

Analyzing Subvisible Particles in Protein Drug Products: a Comparison of Dynamic Light Scattering (DLS) and Resonant Mass Measurement (RMM)

(2014) Jainik Panchal et al.   AAPS Journal

Fc-Optimized NKG2D–Fc Constructs Induce NK Cell Antibody-Dependent Cellular Cytotoxicity against Breast Cancer Cells Independently of HER2/neu Expression Status

(2014) Stefanie Raab et al.   JOURNAL OF IMMUNOLOGY

Effects of Phenol and meta-Cresol Depletion on Insulin Analog Stability at Physiological Temperature

(2014) Brandon M. Teska et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity

(2014) Koen Wagner et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reprogramming the specificity of sortase enzymes

(2014) Brent M. Dorr et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Pharmacokinetics of Peptide–Fc Fusion Proteins

(2013) W.U. Benjamin et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

(2013) Carla P Guimaraes et al.   Nature Protocols

Engineered antibody Fc variant with selectively enhanced Fc RIIb binding over both Fc RIIaR131 and Fc RIIaH131

(2013) F. Mimoto et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Preventing Thiol-Yne Addition Improves the Specificity of Strain-Promoted Azide–Alkyne Cycloaddition

(2012) Remon van Geel et al.   BIOCONJUGATE CHEMISTRY

Rationalizing Nanomaterial Sizes Measured by Atomic Force Microscopy, Flow Field-Flow Fractionation, and Dynamic Light Scattering: Sample Preparation, Polydispersity, and Particle Structure

(2012) M. Baalousha et al.   ENVIRONMENTAL SCIENCE & TECHNOLOGY

Characterization of Fc-Fusion Protein Aggregates Derived from Extracellular Domain Disulfide Bond Rearrangements

(2012) James Strand et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Protein-Protein Fusion Catalyzed by Sortase A

(2011) David A. Levary et al.   PLoS One

A general strategy for the evolution of bond-forming enzymes using yeast display

(2011) I. Chen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Identification of cell culture conditions to control protein aggregation of IgG fusion proteins expressed in Chinese hamster ovary cells

(2011) Ying Jing et al.   PROCESS BIOCHEMISTRY

A variant human IgG1-Fc mediates improved ADCC

(2011) Ross Stewart et al.   PROTEIN ENGINEERING DESIGN & SELECTION

Site-Specific Incorporation ofp-Propargyloxyphenylalanine in a Cell-Free Environment for Direct Protein−Protein Click Conjugation

(2010) Bradley C. Bundy et al.   BIOCONJUGATE CHEMISTRY

Synthesis of N-Terminally Linked Protein Dimers and Trimers by a Combined Native Chemical Ligation-CuAAC Click Chemistry Strategy

(2009) Junpeng Xiao et al.   ORGANIC LETTERS

Aglycosylated IgG variants expressed in bacteria that selectively bind Fc RI potentiate tumor cell killing by monocyte-dendritic cells

(2009) S. T. Jung et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA