The BODIPY-based chemosensor for the fluorometric determination of organochlorine pesticide dicofol

Dicofol is an organochlorine pesticide, which is widely used in fruits, tea and other crops, and is moderately toxic to humans. Therefore, the monitoring of organochlorine pesticide-dicofol is critical for food safety. In this work, a fluorometric chemosensor based on mercaptoethanol and boron dipyrromethene (BODIPY) was first constructed to detect the dicofol. The chemosensor displayed turn-off fluorescence behavior upon dicofol with a detection limit of 200 ppb. The nucleophilicity of the glutathione and other biological thiols was studied to evaluate the reactivity of thiols with dicofol. In practical applications, an obvious color difference was observed on a paper based microfluidic device modified by phenyltriethoxysilane (PTES). We designed an integrated device for pretreatment and paper-based detection, and successfully used for the detection of dicofol in tea. The applicability was demonstrated by detection of dicofol in real tea samples with good recovery ranging from 86% to 109%. The apparatus was convenient and could be used for on-site evaluation of dicofol.

Automated summary

A fluorometric chemosensor based on mercaptoethanol and boron dipyrromethene (BODIPY) was constructed to detect dicofol, showing turn-off fluorescence behavior with a detection limit of 200 ppb. The reactivity of thiols with dicofol was studied and an integrated device for dicofol detection in tea was successfully used with good recovery rates. The apparatus was convenient for on-site evaluation of dicofol in real tea samples.