Journal
FOOD CHEMISTRY
Volume 360, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.130019
Keywords
Phycocyanin; Smartphone; Immunoassay; Aflatoxin B1; On-site determination
Funding
- Fujian Science and Commissioner Technology Project of China
- Fujian Major Science and Technology for Special Program of China [2013NZ0003]
- Fujian Regional Marine Economic Innovation and Development Demonstration Project of China [2014FJ20]
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A new method for quantitative detection of AFB1 in foodstuffs was developed in this study, allowing for rapid on-site testing within 25 minutes. The fluorescence immunochromatographic assay combined phycocyanin fluorescent nanospheres with antiAFB1 monoclonal antibodies to enhance sensitivity and accuracy of detection.
Traditional methods for aflatoxin B1 (AFB1) detection are complex, time-consuming, labor-intensive, and high cost. Moreover, they require sophisticated large-scale instrumentation, which limits their on-site rapid detection. Herein, phycocyanin fluorescent nanospheres based on fluorescence immunochromatographic assay were developed for quantitative detection of AFB1 at parts-per-billion (ppb) levels in foodstuffs. Phycocyanin and antiAFB1 monoclonal antibodies were coupled on the surface of latex nanospheres to amplify the fluorescence signal and improve the sensitivity. The fluorescence intensity was measured by a self-developed smartphone-based reading system. Under the optimal conditions, this approach achieved quantitative point-of-care detection of AFB1 within 25 min. The calibration curve for AFB1 was linear in the range of 0.2-48 ppb, and the limit of detection was 0.16 ppb. The practical applicability of the proposed approach was demonstrated by the determination of AFB1 in naturally contaminated samples, and the results were consistent with HPLC detection.
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