Article
Radiology, Nuclear Medicine & Medical Imaging
Francisco R. Lopez-Picon, Thomas Keller, Diana Bocancea, Jatta S. Helin, Anna Krzyczmonik, Semi Helin, Annelaure Damont, Frederic Dolle, Juha O. Rinne, Merja Haaparanta-Solin, Olof Solin
Summary: In this study, the newly developed TSPO tracer [F-18]F-DPA showed higher brain uptake, higher TG-to-WT SUVRCB ratios, and faster clearance compared to [F-18]DPA-714 and [C-11]PBR28. These findings suggest that [F-18]F-DPA could be a useful tool for detecting low levels of inflammation and allowing for shorter dynamic PET scans.
MOLECULAR IMAGING AND BIOLOGY
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
M. A. Peyronneau, B. Kuhnast, D-L Nguyen, B. Jego, G. Sayet, F. Caille, S. Lavisse, P. Gervais, B. Stankoff, M. Sarazin, P. Remy, V Bouilleret, C. Leroy, M. Bottlaender
Summary: This study aimed to assess the impact of concomitant medication, age, sex, body mass index, and TSPO binding affinity on the metabolism and plasma pharmacokinetics of [F-18]DPA-714 and their influence on the plasma input function. The results showed significant correlations between concomitant medication, age, sex, body mass index, and [F-18]DPA-714 metabolism and concentration.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Neurosciences
Jiamei Guo, Tian Qiu, Lixia Wang, Lei Shi, Ming Ai, Zhu Xia, Zhiping Peng, Anhai Zheng, Xiao Li, Li Kuang
Summary: In this study, the role of glial cells in stress-induced depression was investigated. The findings suggest that changes in glial cells may contribute to the development and symptoms of depression.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Anesthesiology
Christina Mueller, Yu-Hua D. Fang, Chloe Jones, Jonathan E. Mcconathy, Fabio Raman, Suzanne E. Lapi, Jarred W. Younger
Summary: This study found increased binding of the translocator protein radioligand in several brain regions among fibromyalgia patients. The results suggest that individuals with fibromyalgia may have higher levels of neuroinflammation than healthy controls. Additionally, the study found higher binding levels in multiple brain regions among high-affinity binders in the fibromyalgia group. The differences in the right parietal gray matter were associated with decreased quality of life, higher pain severity and interference, and cognitive problems.
Article
Immunology
Charles L. Howe, Reghann G. LaFrance-Corey, Brittany L. Overlee, Renee K. Johnson, Benjamin D. S. Clarkson, Emma N. Goddery
Summary: This study investigated the relative contribution of inflammatory monocytes and microglia in ictogenesis using the Theiler's murine encephalomyelitis virus (TMEV) model. The results showed that the microglial response did not scale with the inoculum, while the inflammatory monocyte response scaled with the amount of infectious virus. Inoculation conditions that drove inflammatory monocyte infiltration resulted in severe seizures and EEG abnormalities, while low-level inoculation conditions associated with microglial activation resulted in milder seizures and EEG phenotypes.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Immunology
Marta Vicente-Rodriguez, Nisha Singh, Federico Turkheimer, Alba Peris-Yague, Karen Randall, Mattia Veronese, Camilla Simmons, Abdul Karim Haji-Dheere, Jayanta Bordoloi, Kerstin Sander, Ramla O. Awais, Erik Arstad, Nima Consortium, Diana Cash, Christine A. Parker
Summary: By utilizing TSPO PET imaging, we can detect peripherally-induced neuroinflammation in the central nervous system, primarily arising from a combination of microglia, astrocytes, and monocyte-derived macrophages.
BRAIN BEHAVIOR AND IMMUNITY
(2021)
Article
Multidisciplinary Sciences
Lauren Mifflin, Zhirui Hu, Connor Dufort, Cynthia C. Hession, Alec J. Walker, Kongyan Niu, Hong Zhu, Nan Liu, Jun S. Liu, Joshua Z. Levin, Beth Stevens, Junying Yuan, Chengyu Zou
Summary: The study identifies a subclass of microglia, termed RIPK1-Regulated Inflammatory Microglia (RRIMs), in mouse models of ALS. RRIMs show significant up-regulation of proinflammatory pathways, are highly regulated by TNF alpha signaling, and their prevalence can be suppressed by inhibiting RIPK1 activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Neurosciences
Jun Wang, Jingxue Liang, Jiahong Deng, Xiaolin Liang, Kewan Wang, Hongxiao Wang, Dadi Qian, Hao Long, Kaijun Yang, Songtao Qi
Summary: Epilepsy is a common and serious complication of subarachnoid hemorrhage (SAH), and the role of neuroinflammation, characterized by microglial activation and release of inflammatory cytokines, in epilepsy after SAH requires further investigation.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Immunology
Emilia A. Gaviglio, Javier M. Peralta Ramos, Daniela S. Arroyo, Claudio Bussi, Pablo Iribarren, Maria C. Rodriguez-Galan
Summary: The development of neuroinflammation and progression of neurodegenerative diseases are associated with the activation and mobilization of the peripheral immune system due to systemic inflammation. This study investigated the effects of systemic sterile induced-co-expression of IL-12 and IL-18 on the establishment of a cytokine-mediated model of neuroinflammation.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Clinical Neurology
Tianyuan Wang, Huandi Weng, Hongji Zhou, Zecheng Yang, Zhongyou Tian, Biao Xi, Yalan Li
Summary: This study aimed to investigate the protective effects and mechanisms of action of esketamine in postoperative depression (POD). The results showed that esketamine can significantly improve depression-like symptoms caused by anesthesia and surgery, and restore synaptogenesis in the medial prefrontal cortex (mPFC) that had been perturbed by inflammation. The anti-inflammatory effects of esketamine may be mediated by the BDNF/TrkB signaling pathway.
JOURNAL OF AFFECTIVE DISORDERS
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Cristina Barca, Amanda J. Kiliaan, Lydia Wachsmuth, Claudia Foray, Sven Hermann, Cornelius Faber, Michael Schaefers, Maximilian Wiesmann, Bastian Zinnhardt, Andreas H. Jacobs
Summary: This study investigated the effects of microglia repopulation on inflammation and functional outcomes in an ischemic mouse model. The results showed that microglia repopulation can resolve the inflammatory imbalance and contribute to functional recovery after ischemia.
JOURNAL OF NUCLEAR MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Stefania Della Vecchia, Maria Marchese, Filippo Maria Santorelli
Summary: This study systematically reviewed the role of glial cells in Lafora disease (LD). The findings suggest that glial cells accumulate polyglucosan bodies (PBs) in LD and contribute to neurodegeneration and epilepsy. Future research should consider glial cells as a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Peizhen Ye, Lei Bi, Min Yang, Yifan Qiu, Guolong Huang, Yongshan Liu, Yuyi Hou, Zhijun Li, Henry Hoi Yee Tong, Mengchao Cui, Hongjun Jin
Summary: In this study, the expression of TSPO was measured using [18F]DPA-714 positron emission tomography-magnetic resonance imaging (PET-MRI) to investigate the effects of microglial activation associated with motor behavioral impairments in a rodent model of Parkinson's disease (PD). The results showed elevated TSPO binding in the striatum during the first three weeks post-treatment, indicating potential neuroinflammation in the early stage of PD. [18F]DPA-714 could be a potential PET tracer for imaging microglia-mediated neuroinflammation in early PD.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Immunology
Kuriko Kagitani-Shimono, Hiroki Kato, Ryoko Kuwayama, Koji Tominaga, Shin Nabatame, Haruhiko Kishima, Jun Hatazawa, Masako Taniike
Summary: Neuroinflammation, visualized using translocator protein positron emission tomography with [C-11] DPA713, was found to be sensitive in identifying epileptic foci in child-onset focal epilepsy. The inflammation was implicated in the pathophysiology of epileptic foci caused by various etiologies, indicating a need for further research in diagnostic tools for identifying focal epileptogenic zones.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Biochemistry & Molecular Biology
Peizhen Ye, Lei Bi, Min Yang, Yifan Qiu, Guolong Huang, Yongshan Liu, Yuyi Hou, Zhijun Li, Henry Hoi Yee Tong, Mengchao Cui, Hongjun Jin
Summary: In this study, TSPO expression was investigated using [18F]DPA-714 positron emission tomography-magnetic resonance imaging (PET-MRI) to study microglial activation and motor behavioral impairments in a rodent model of Parkinson's disease (PD) treated with 6-hydroxydopamine (6-OHDA). The results showed an increase in striatal [18F]DPA-714 binding ratio during the first three weeks post-treatment, with the peak TSPO binding in the first week. There was a significant correlation between [18F]DPA-714 SUVRR/L and rotation numbers, indicating the potential of [18F]DPA-714 as a PET tracer for imaging microglia-mediated neuroinflammation in early PD.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Satoshi Nozaki, Aya Mawatari, Yuka Nakatani, Emi Hayashinaka, Yasuhiro Wada, Yukihiro Nomura, Takahito Kitayoshi, Kouji Akimoto, Shinji Ninomiya, Hisashi Doi, Yasuyoshi Watanabe
MOLECULAR IMAGING AND BIOLOGY
(2018)
Article
Biochemical Research Methods
Miki Goto, Akira Nishiyama, Takao Yamaguchi, Kyosuke Watanabe, Kenji Fujii, Yasuyoshi Watanabe, Hisashi Doi
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS
(2019)
Article
Multidisciplinary Sciences
Kayo Takahashi, Takamitsu Hosoya, Kayo Onoe, Tadayuki Takashima, Masaaki Tanaka, Akira Ishii, Yasuhito Nakatomi, Shusaku Tazawa, Kazuhiro Takahashi, Hisashi Doi, Yasuhiro Wada, Yasuyoshi Watanabe
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Kyosuke Watanabe, Satoshi Nozaki, Mild Goto, Ken-ichi Kaneko, Emi Hayashinaka, Satsuki Irie, Akira Nishiyama, Kazuaki Kasai, Kenji Fujii, Yasuhiro Wada, Kei Mizuno, Kenji Mizuseki, Hisashi Doi, Yasuyoshi Watanabe
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Biochemical Research Methods
Takayuki Ose, Joonas A. Autio, Masahiro Ohno, Kantaro Nishigori, Nobuyoshi Tanki, Ami Igesaka, Tomoko Mori, Hisashi Doi, Yasuhiro Wada, Iwao Nakajima, Hiroshi Watabe, Takuya Hayashi
JOURNAL OF NEUROSCIENCE METHODS
(2019)
Article
Multidisciplinary Sciences
Satoshi Nozaki, Yuka Nakatani, Aya Mawatari, Nina Shibata, William E. Hume, Emi Hayashinaka, Yasuhiro Wada, Hisashi Doi, Yasuyoshi Watanabe
SCIENTIFIC REPORTS
(2019)
Correction
Biochemistry & Molecular Biology
Yasuyoshi Watanabe, Aya Mawatari, Kazuki Aita, Yuzuru Sato, Yasuhiro Wada, Takayoshi Nakaoka, Kayo Onoe, Emi Yamano, Go Akamatsu, Akihito Ohnishi, Keiji Shimizu, Masahiro Sasaki, Hisashi Doi, Michio Senda
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yasuyoshi Watanabe, Aya Mawatari, Kazuki Aita, Yuzuru Sato, Yasuhiro Wada, Takayoshi Nakaoka, Kayo Onoe, Emi Yamano, Go Akamatsu, Akihito Ohnishi, Keiji Shimizu, Masahiro Sasaki, Hisashi Doi, Michio Senda
Summary: This study investigated the whole-body pharmacokinetics of [C-11]TTFD in human healthy volunteers using PET imaging and radiation dosimetry. The results showed different uptake patterns of the compound in various tissues, with a relatively low effective radiation dose calculated.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Medicinal
Shuhei Takatani, Tsuyoshi Tahara, Mieko Tsuji, Daiki Ozaki, Nina Shibata, Yoshinobu Hashizume, Masaaki Suzuki, Hirotaka Onoe, Yasuyoshi Watanabe, Hisashi Doi
Summary: Efficient synthesis of L-[5-C-11]leucine and L-alpha-[5-C-11]methylleucine was achieved using a two-step sequence, with promising potential of L-alpha-[5-C-11]methylleucine as a probe for visualizing tumors confirmed through tissue distribution analysis.
Article
Chemistry, Organic
Hisashi Doi, Miki Goto, Yuzuru Sato
Summary: A rapid Pd-0-mediated cross-coupling reaction was reported for synthesizing C-11-acetic acid and its corresponding imidyl esters as radiolabeling reagents. The reaction yielded [C-11]2 with satisfactory radioactivity and good yield, suitable for in vivo imaging studies. The injectable radiopharmaceutical formulation of [C-11]2 prepared using 33 GBq of [C-11]CH3I showed a decay-corrected radiochemical yield of 63-73%, providing potential applications in positron emission tomography studies.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Takayoshi Nakaoka, Ken-ichi Kaneko, Satsuki Irie, Aya Mawatari, Ami Igesaka, Yuta Uetake, Hidenori Ochiai, Takashi Niwa, Emi Yamano, Yasuhiro Wada, Masaaki Tanaka, Kohei Kotani, Hideki Kawahata, Joji Kawabe, Yukio Miki, Hisashi Doi, Takamitsu Hosoya, Maeda Kazuya, Hiroyuki Kusuhara, Yuichi Sugiyama, Yasuyoshi Watanabe
Summary: Uptake and efflux transporters play crucial roles in drug disposition. PET imaging is a useful tool for directly assessing drug transporter activity. A synthetic method for [18F]PTV, a PET probe for hepatobiliary transport, was developed. Rifampicin pretreatment significantly inhibited hepatic concentration and biliary excretion of the probe and decreased hepatic uptake clearance and canalicular efflux clearance. These results demonstrate the usefulness of [18F]PTV for investigating hepatobiliary transporters in humans.
DRUG METABOLISM AND PHARMACOKINETICS
(2022)
Article
Multidisciplinary Sciences
Kayo Takahashi, Takamitsu Hosoya, Kayo Onoe, Tomoko Mori, Shusaku Tazawa, Aya Mawatari, Yasuhiro Wada, Yumiko Watanabe, Hisashi Doi, Yasuyoshi Watanabe
Summary: This study synthesized three analogs of cetrozole for PET imaging of aromatase in the brain, with iso-cetrozole showing the most promising results. The inhibitory activities of the analogs correlated well with their binding potentials to aromatase in animal models, and iso-cetrozole demonstrated faster metabolism in brain tissue compared to cetrozole.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Satoshi Nozaki, Yuka Nakatani, Aya Mawatari, William E. Hume, Yasuhiro Wada, Akira Ishii, Masaaki Tanaka, Naohiro Tsuyuguchi, Hisashi Doi, Yasuyoshi Watanabe
Summary: In this first-in-human PET study, the biodistribution and tumor accumulation of a novel PET probe, F-18-FIMP, were evaluated. The results showed rapid excretion of F-18-FIMP from the kidneys and no characteristic physiological accumulation in healthy controls. Compared with other probes, F-18-FIMP provided clearer imaging in patients with brain tumors.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Tatsuya Kida, Nobuaki Takahashi, Masayuki X. Mori, Jiacheng H. Sun, Hideto Oota, Kosuke Nishino, Takashi Okauchi, Yuta Ochi, Daisuke Kano, Ukihide Tateishi, Yasuyoshi Watanabe, Yilong Cui, Yasuo Mori, Hisashi Doi
Summary: TRPV1-targeted compounds were synthesized by modifying the structure of SB366791, and higher antagonistic activities were achieved by avoiding amide-iminol tautomerization. Additionally, explorative PET imaging trials were conducted using labeled derivatives, providing a basis for further applications.
RSC MEDICINAL CHEMISTRY
(2022)