Improving the low-temperature properties of an exo-inulinase via the deletion of a loop fragment located in its catalytic pocket

A simplified method to remove fusion tags from a xylanase of Bacillus sp. HBP8 with HRV 3C protease

(2019) Hu Xu et al.   ENZYME AND MICROBIAL TECHNOLOGY

Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability

(2019) Junyan Ma et al.   Journal of Biological Engineering

Recent advances in bio-based multi-products of agricultural Jerusalem artichoke resources

(2018) Yibin Qiu et al.   Biotechnology for Biofuels

Enhanced Purification Efficiency and Thermal Tolerance of Thermoanaerobacterium aotearoense β-Xylosidase through Aggregation Triggered by Short Peptides

(2018) Tianwang Xu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Insight into the Thermophilic Mechanism of a Glycoside Hydrolase Family 5 β-Mannanase

(2018) Weina Liu et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Exploiting correlated molecular-dynamics networks to counteract enzyme activity–stability trade-off

(2018) Haoran Yu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biotechnological applications of inulin-rich feedstocks

(2018) R.S. Singh et al.   BIORESOURCE TECHNOLOGY

A panorama of bacterial inulinases: Production, purification, characterization and industrial applications

(2017) Ram Sarup Singh et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Loops Adjacent to Catalytic Region and Molecular Stability of Man1312

(2016) Haiyan Zhou et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Enhanced exo-inulinase activity and stability by fusion of an inulin-binding module

(2016) Shun-Hua Zhou et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Defying the activity–stability trade-off in enzymes: taking advantage of entropy to enhance activity and thermostability

(2016) Khawar Sohail Siddiqui   CRITICAL REVIEWS IN BIOTECHNOLOGY

Deletion of loop fragment adjacent to active site diminishes the stability and activity of exo-inulinase

(2016) Maryam Rezaei Arjomand et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Discovery, Molecular Mechanisms, and Industrial Applications of Cold-Active Enzymes

(2016) Margarita Santiago et al.   Frontiers in Microbiology

Probing the role of cation-π interaction in the thermotolerance and catalytic performance of endo-polygalacturonases

(2016) Tao Tu et al.   Scientific Reports

Some like it hot, some like it cold: Temperature dependent biotechnological applications and improvements in extremophilic enzymes

(2015) Khawar Sohail Siddiqui   BIOTECHNOLOGY ADVANCES

Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization

(2015) Longmei Zhao et al.   BMC BIOTECHNOLOGY

Cold-active and NaCl-tolerant exo-inulinase from a cold-adapted Arthrobacter sp. MN8 and its potential for use in the production of fructose at low temperatures

(2015) Junpei Zhou et al.   JOURNAL OF BIOSCIENCE AND BIOENGINEERING

Shortening a loop can increase protein native state entropy

(2015) Yulian Gavrilov et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

A novel low-temperature-active exo-inulinase identified based on Molecular-Activity strategy from Sphingobacterium sp. GN25 isolated from feces of Grus nigricollis

(2014) Junpei Zhou et al.   PROCESS BIOCHEMISTRY

Engineering proteins for thermostability through rigidifying flexible sites

(2013) Haoran Yu et al.   BIOTECHNOLOGY ADVANCES

Deletion of a dynamic surface loop improves stability and changes kinetic behavior of phosphatidylinositol-synthesizingStreptomycesphospholipase D

(2013) Jasmina Damnjanović et al.   BIOTECHNOLOGY AND BIOENGINEERING

Stabilization of a protein conferred by an increase in folded state entropy

(2013) S. Dagan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Production and Properties of Microbial Inulinases: Recent Advances

(2011) Naveen Kango et al.   FOOD BIOTECHNOLOGY

PROPKA3: Consistent Treatment of Internal and Surface Residues in Empirical pKaPredictions

(2011) Mats H. M. Olsson et al.   Journal of Chemical Theory and Computation