Article
Biophysics
Victor Nicolai Friedhoff, Benjamin Lindner, Martin Falcke
Summary: This article investigates the randomness and properties of Ca2+ signaling in cells. The researchers found that the generation of Ca2+ spikes follows certain patterns and can be described as a process from one state to another. They proposed a theoretical model to explain the differences in spike behavior between different cell types and stimulating agonists, as well as the variability observed in experiments.
BIOPHYSICAL JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Roberto Docampo, Guozhong Huang
Summary: Trypanosoma cruzi and the T. brucei group of parasites are neglected diseases affecting millions of people globally. These microorganisms have complex life cycles involving insect vectors and mammalian hosts, and possess unique signaling pathways and receptor adaptations that differentiate them from their mammalian counterparts.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Review
Neurosciences
Kjell Heuser, Rune Enger
Summary: Epilepsy is a common neurological disorder affecting millions worldwide, with current research focusing on neuronal discharges and the role of astrocytes in releasing signaling substances that interact with neurons. The central role of astrocytic Ca2+ signals in promoting ictogenesis and epileptogenesis is increasingly recognized and studied.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Cell Biology
Irene Vorontsova, Jeffrey T. Lock, Ian Parker
Summary: KRAP is crucial for the appropriate localization and functioning of IP3Rs, mediating the release of Ca2+ from the endoplasmic reticulum. It regulates the activity of IP3R channels at puff sites and determines the functioning of individual channels in a stochastic manner. KRAP is also essential for the functioning of IP3Rs involved in diffuse Ca2+ release.
Article
Multidisciplinary Sciences
Wataru Okumura, Takuma Kozono, Hiroyuki Sato, Hitomi Matsui, Tsubasa Takagi, Takashi Tonozuka, Atsushi Nishikawa
Summary: This study demonstrates that Jaw1 increases calcium influx by changing the oscillation pattern and enhancing calcium release activity of ITPR subtypes upon GPCR stimulation. The findings provide insights into the relationship between heterogeneous calcium dynamics and specific ITPR and Jaw1 expression patterns.
SCIENTIFIC REPORTS
(2022)
Article
Physics, Multidisciplinary
Victor Nicolai Friedhoff, Lukas Ramlow, Benjamin Lindner, Martin Falcke
Summary: In cellular biophysics, the complexity and limited knowledge make it impractical to write down equations completely, so hypotheses-based modelling is used instead. Two textbook systems, Ca2+ signalling and neuronal membrane potential dynamics, both involve stochastic spiking and non-equilibrium features, requiring predictive models beyond experimental examples. Recent progress has been made in describing Ca2+ spikes and neuronal spike trains using stochastic models and concepts from computational neuroscience, which may help understand long-term memory effects in Ca2+ spiking.
EUROPEAN PHYSICAL JOURNAL-SPECIAL TOPICS
(2021)
Article
Biophysics
Lukas Ramlow, Martin Falcke, Benjamin Lindner
Summary: In computational neuroscience, integrate-and-fire models are used to describe spike generation in both single cells and networks efficiently and analytically. Stochastic spiking is also observed in Ca2+ signaling, making the integrate-and-fire approach applicable. This study introduces a two-component model for describing the activity of inositol-trisphosphate receptor channels and the dynamics of cytosolic Ca2+ concentrations. The model allows for fast numerical simulations, analytical expressions for spiking rates and interspike interval statistics, and approximations of the interval density and power spectrum. Experimental data are compared to the model's statistics.
BIOPHYSICAL JOURNAL
(2023)
Article
Physiology
Allison Tambeaux, Yuriana Aguilar-Sanchez, Demetrio J. Santiago, Madeleine Mascitti, Karyn M. DiNovo, Rafael Mejia-Alvarez, Michael Fill, S. R. Wayne Chen, Josefina Ramos-Franco
Summary: The D2594K mutation in the IP3R1 receptor enhances ligand sensitivity and alters the response to cytosolic and luminal Ca2+ levels.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Nicolas Rosa, Femke Speelman-Rooms, Jan B. Parys, Geert Bultynck
Summary: Members of the Bcl-2 protein family play important roles in controlling apoptotic cell death. Beyond their well-known functions in the mitochondria, these proteins also modulate intracellular Ca2+ signals originating from the endoplasmic reticulum. Recent studies have revealed new insights into the functions and interactions of Bcl-2 and Bcl-xL, opening up new possibilities for the treatment of Bcl-xL-dependent cancers.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2022)
Article
Developmental Biology
Rishav Mitra, Shlesha Richhariya, Siddharth Jayakumar, Dimple Notani, Gaiti Hasan
Summary: In fruit fly larvae, the IP3/Ca2+ signals in specific interneurons drive the expression of Set2 gene, essential for pupariation in the absence of dietary protein. This signal-controlled expression of Set2 upregulates key Ca2+-signaling genes through a positive-feedback loop, regulating gene expression for the transition from larval to pupal stages.
Article
Plant Sciences
Bin Li, Liyuan Hou, Chenggang Song, Zhengbiao Wang, Qiyang Xue, Yuanyang Li, Jianchun Qin, Ning Cao, Chengguo Jia, Yubin Zhang, Wuliang Shi
Summary: This article provides an update on the multiple roles of the calcium-sensing receptor (CAS) in regulating stomatal movement and calcium signaling in plants. It also discusses the possible phosphorylation mechanism of CAS and its involvement in abiotic and biotic stresses.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Felix Hohendanner, Ashok Prabhu, Nicola Wilck, Verena Stangl, Burkert Pieske, Karl Stangl, Till F. Althoff
Summary: G(q)-signaling promotes arrhythmogenic atrial Ca2+-release and atrial fibrillation (AF). Targeting this pathway, preferably using G(q)-selective receptor ligands, may be a promising approach for the treatment and prevention of AF, while avoiding adverse effects on the ventricles.
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Article
Physiology
Adan Dagnino-Acosta, Agustin Guerrero-Hernandez
Summary: PKC inhibitors stimulate Ca2+ release from internal stores by increasing SR Ca2+ leak mediated by translocon activation. This increased leak does not deplete the Ca2+ store, but rather compensates by increasing SERCA pump activity, leading to a new steady-state level. This change also results in increased activity of high conductance, Ca2+-sensitive potassium channels.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhenni Liu, Xue Wu, Qi Wang, Zixuan Li, Xueqi Liu, Xiaodong Sheng, Hong Zhu, Mengda Zhang, Junrui Xu, Xiaowen Feng, Baoming Wu, Xiongwen Lv
Summary: The CD73-A(1)R axis can regulate the activation and apoptosis of HSCs through the PLC-IP3-Ca2+/DAG-PKC signaling pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Jeffrey T. Lock, Ian Parker, Ian F. Smith
Article
Multidisciplinary Sciences
Jeffrey T. Lock, Kyle L. Ellefsen, Bret Settle, Ian Parker, Ian F. Smith
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2015)
Article
Cell Biology
Jeffrey T. Lock, Ian Parker, Ian F. Smith
Article
Cardiac & Cardiovascular Systems
Jeffrey T. Lock, William G. Sinkins, William P. Schilling
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Bassam G. Abu Jawdeh, Shenaz Khan, Isabelle Deschenes, Malcolm Hoshi, Monu Goel, Jeffrey T. Lock, Krekwit Shinlapawittayatorn, Gerald Babcock, Sujata Lakhe-Reddy, Garren DeCaro, Satya P. Yadav, Maradumane L. Mohan, Sathyamangla V. Naga Prasad, William P. Schilling, Eckhard Ficker, Jeffrey R. Schelling
JOURNAL OF BIOLOGICAL CHEMISTRY
(2011)
Article
Neurosciences
Jeffrey T. Lock, William G. Sinkins, William P. Schilling
JOURNAL OF PHYSIOLOGY-LONDON
(2012)
Article
Biochemistry & Molecular Biology
Kyle L. Ellefsen, Jeffrey T. Lock, Brett Settle, Carley A. Karsten, Ian Parker
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Jeffrey T. Lock, Kamil J. Alzayady, David I. Yule, Ian Parker
Review
Cell Biology
Jeffrey T. Lock, Ian F. Smith, Ian Parker
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2019)
Article
Biology
Jeffrey T. Lock, Ian Parker
Article
Biology
Horia Vais, Min Wang, Karthik Mallilankaraman, Riley Payne, Chris McKennan, Jeffrey T. Lock, Lynn A. Spruce, Carly Fiest, Matthew Yan-lok Chan, Ian Parker, Steven H. Seeholzer, J. Kevin Foskett, Don-On Daniel Mak
Article
Cell Biology
Jeffrey T. Lock, Ian F. Smith, Ian Parker
Article
Oncology
Stephen M. Selkirk, Jay Morrow, Tara A. Barone, Alan Hoffer, Jeffrey Lock, Anne DeChant, Saisho Mangla, Robert J. Plunkett, Robert H. Miller
JOURNAL OF NEURO-ONCOLOGY
(2008)
Article
Cell Biology
Dominic Tscherrig, Rajesh Bhardwaj, Daniel Biner, Jan Dernic, Daniela Ross-Kaschitza, Christine Peinelt, Matthias A. Hediger, Martin Lochner
Summary: Changes in Ca2+ levels regulate many physiological functions, and the endoplasmic reticulum is the major Ca2+ store in cells. Store-operated calcium entry (SOCE) is a refilling process that involves protein-protein interactions between Ca2+ sensing STIM in the ER and Orai proteins in the plasma membrane, forming selective Ca2+ channels. Abnormal SOCE is associated with diseases and cancers, making STIM and Orai important therapeutic targets. Novel GSK-7975A-based molecular probes have been developed for studying SOCE in different cells and settings.
Article
Cell Biology
Nicola Fameli, Cornelis van Breemen, Klaus Groschner
Summary: The study provides evidence for the importance of junctional membrane architecture in cellular function. Through modeling and prediction, it is shown that nano-scale membrane spacing plays a crucial role in junctional ER Ca2+ refilling and signaling efficiency. Additionally, interactions between Ca2+ and the phospholipid membrane surface are suggested to support interfacial Ca2+ transport and receptor targeting. This research introduces a new concept in pathophysiology.
Article
Cell Biology
Satomi Ohtsuka, Yumi Miyai, Hiroyuki Mima, Masaki Magari, Yoichi Chiba, Futoshi Suizu, Hiroyuki Sakagami, Masaki Ueno, Hiroshi Tokumitsu
Summary: This study characterized the splice variants of mouse CaMKKI3/2 and found that CaMKKI3-3 and I3-3x were functionally active and likely to be bona fide CaMKIV kinases involved in the regulation of spermiogenesis.