Review
Biochemistry & Molecular Biology
Jing Cui, Weida Shen, Haiyan Zhao
Summary: Obesity has been recognized as an independent risk factor for diseases such as inflammation, cardiovascular disease, and cancer. Adipocytes, found in diverse tissues, play important roles in homeostasis and disease progression. Adipose tissue functions not only as an energy organ but also as an endocrine organ that communicates with other cells in the microenvironment. This review focuses on the functions of breast cancer-associated adipose tissue-derived extracellular vesicles (EVs) in breast cancer progression, including proliferation, metastasis, drug resistance, and immune regulation. Understanding the role of EVs in the interaction between adipocytes and breast cancer can enhance our knowledge of cancer biology and progression, leading to improved diagnostic strategies and therapeutic insights.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2023)
Article
Medicine, Research & Experimental
Ines Barone, Luca Gelsomino, Felice Maria Accattatis, Francesca Giordano, Balazs Gyorffy, Salvatore Panza, Mario Giuliano, Bianca Maria Veneziani, Grazia Arpino, Carmine De Angelis, Pietro De Placido, Daniela Bonofiglio, Sebastiano Ando, Cinzia Giordano, Stefania Catalano
Summary: Obesity is a known risk factor for various chronic diseases, including breast cancer. This study found that the expression levels of miR-126-3p, miR-4772-3p, and miR-10a-5p in circulating extracellular vesicles were negatively correlated with survival in obese breast cancer patients.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Zhaowei Sun, Dong Sun, Yujie Feng, Bingyuan Zhang, Peng Sun, Bin Zhou, Lutao Du, Yunshan Wang, Zhiyao Fan, Jian Yang, Yongzheng Li, Sanyuan Hu, Hanxiang Zhan
Summary: Exosomes derived from pancreatic cancer cells contain linc-ROR, which can induce dedifferentiation of adipocytes and promote pancreatic cancer cell EMT via the HIF1α-ZEB1 axis. Exosomal linc-ROR may serve as a novel diagnostic marker for pancreatic cancer patients.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2021)
Article
Oncology
Man Luo, Junqi Zhu, Jie Ren, Yuxiao Tong, Limin Wang, Shenglin Ma, Jiaoli Wang
Summary: Lactate promotes tumor progression by enhancing the production of TEVs through decreasing intracellular cAMP levels and PKA activation. Serum lactate levels are positively correlated with serum EV levels and Rab27a and HIF-1 alpha protein levels in lung cancer patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Jie Wang, Hao Wu, Yue Zhao, Youyou Qin, Yingbo Zhang, Hao Pang, Yongting Zhou, Xueyi Liu, Zhibo Xiao
Summary: In this study, the overexpression of HIF-1α in ADSCs-hEVs was investigated for its mechanism in diabetic wound repair. The findings showed that ADSCs-hEVs significantly increased the expression and secretion of growth factors, leading to accelerated wound healing and improved healing quality in diabetic wounds.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Cell Biology
Xiaoning Wang, Ruoyi Wu, Peisong Zhai, Zheqi Liu, Ronghui Xia, Zhen Zhang, Xing Qin, Chuwen Li, Wantao Chen, Jiang Li, Jianjun Zhang
Summary: Tumour cells under hypoxia increase the release of extracellular vesicles, specifically the endosome-derived subtype, due to the downregulation of ATP6V1A expression by hypoxia-inducible factor-1 alpha (HIF-1 alpha). This leads to impaired lysosomal degradation and reduced fusion of multivesicular bodies with lysosomes, allowing the secretion of intraluminal vesicles as extracellular vesicles. These findings establish a HIF-1 alpha-regulated lysosomal dysfunction-extracellular vesicle release axis and contribute to the understanding of extracellular vesicle biogenesis.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Cell Biology
Zhiguo Niu, Jianing Fan, Fengzhe Chen, Huijie Yang, Xin Li, Ting Zhuang, Chunlei Guo, Qi Cao, Jian Zhu, Hui Wang, Qingsong Huang
Summary: Breast cancer is the most common malignancy in women globally. Abnormal expression of RBCK1 protein in breast tumors makes it a valuable diagnostic marker and drug target. Hypoxic regions in breast cancer activate the HIF1 pathway, influencing tumor recurrence and metastasis. Targeting RBCK1 could be a promising treatment strategy for ER-positive breast cancer.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Wang, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: This study reveals that UBE3B is an E3 ligase for HIF-2 & alpha; and its depletion inhibits breast cancer progression. UBE3B physically interacts with HIF-2 & alpha; and promotes its polyubiquitination, thereby inhibiting its degradation. UBE3B depletion also inhibits breast cancer cell proliferation, migration, and invasion, and suppresses tumor growth and metastasis.
Article
Biochemistry & Molecular Biology
Yufan Qiu, Yi Yang, Riyao Yang, Chunxiao Liu, Jung-Mao Hsu, Zhou Jiang, Linlin Sun, Yongkun Wei, Chia-Wei Li, Dihua Yu, Jin Zhang, Mien-Chie Hung
Summary: The study reveals that PD-1 can be secreted in an exosomal form by activated T cells and interact remotely with PD-L1. This interaction can attenuate the suppression of tumor-specific cytotoxic T cell activity by PD-L1, restoring tumor surveillance.
Article
Biochemistry & Molecular Biology
Yijie Wang, Xiong Liu, Min Liu, Yu Wang, Shuo Wang, Lai Jin, Min Liu, Jun Zhou, Yan Chen
Summary: It has been found that UBE3B acts as an E3 ligase for HIF-2 alpha, promoting its polyubiquitination and inhibiting its degradation, which plays a crucial role in breast cancer growth and metastasis.
Article
Biochemical Research Methods
Wenwen Chen, Rongkai Cao, Wentao Su, Xu Zhang, Yuhai Xu, Peng Wang, Zhongqiao Gan, Yingying Xie, Hongjing Li, Jianhua Qin
Summary: The study proposes a simple, quick, and label-free method for isolating circulating exosomes from patient serum. Further validation through miRNA sequencing and RT-PCR reveals a potential new biomarker.
Article
Biochemistry & Molecular Biology
Shuchen Liu, Alberto Benito-Martin, Fanny A. Pelissier Vatter, Sarah Z. Hanif, Catherine Liu, Priya Bhardwaj, Praveen Sethupathy, Alaa R. Farghli, Phoebe Piloco, Paul Paik, Malik Mushannen, Xue Dong, David M. Otterburn, Leslie Cohen, Rohan Bareja, Jan Krumsiek, Leona Cohen-Gould, Samuel Calto, Jason A. Spector, Olivier Elemento, David C. Lyden, Kristy A. Brown
Summary: Breast adipose tissue-derived extracellular vesicles (EVs) from overweight or obese women can promote breast cancer cell proliferation and induce metabolic reprogramming. Several miRNAs enriched in these EVs are responsible for enhancing mitochondrial respiration and proliferation. This study reveals a new aspect of the obesity-breast cancer link and highlights the role of EVs in modulating tumor cell functions.
Article
Biochemistry & Molecular Biology
Tommaso Montecchi, Enxhi Shaba, Domiziana De Tommaso, Fabrizio Di Giuseppe, Stefania Angelucci, Luca Bini, Claudia Landi, Cosima Tatiana Baldari, Cristina Ulivieri
Summary: Deficiency of the molecular adaptor Rai has been shown to reduce disease severity in experimental autoimmune encephalomyelitis. Rai contributes to the generation of a pro-inflammatory CNS microenvironment in astrocytes. Proteomic analysis revealed that Rai impacts astrocyte function and regulates multiple molecular pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yongkang Yang, Chelsey Chen, Qiaozhu Zuo, Haiquan Lu, Shaima Salman, Yajing Lyu, Tina Yi-Ting Huang, Elizabeth E. Wicks, Walter Jackson III, Emmanuel Datan, Ru Wang, Yufeng Wang, Nguyet Le, Yayun Zhu, Wenxin Qin, Gregg L. Semenza
Summary: Hypoxia in the breast cancer microenvironment leads to increased expression of HIF-1, which is associated with poor patient outcome. In this study, NARF was identified as a hypoxia-inducible, HIF-1 target gene in human breast cancer cells. Knockdown of NARF significantly reduced the population of breast cancer stem cells and impaired tumor initiation capacity and lung metastasis in mouse models.
Article
Nutrition & Dietetics
Young Eun Cho, Rany Vorn, Michael Chimenti, Keith Crouch, Chen Shaoshuai, Janhavi Narayanaswamy, Alaria Harken, Reegan Schmidt, Jessica Gill, Hyangkyu Lee
Summary: In this study, we investigated the altered miRNAs in bEVs of mothers with obesity and their potential impact on breastfed infants.
FRONTIERS IN NUTRITION
(2022)
Article
Oncology
Valentina Maggisano, Marilena Celano, Rocco Malivindi, Ines Barone, Donato Cosco, Catia Mio, Chiara Mignogna, Salvatore Panza, Giuseppe Damante, Massimo Fresta, Sebastiano Ando, Diego Russo, Stefania Catalano, Stefania Bulotta
Article
Pharmacology & Pharmacy
Ortensia Ilaria Parisi, Mariarosa Ruffo, Rocco Malivindi, Anna Francesca Vattimo, Vincenzo Pezzi, Francesco Puoci
Article
Endocrinology & Metabolism
Vittoria Rago, Daniela De Rose, Marta Santoro, Salvatore Panza, Rocco Malivindi, Sebastiano Ando, Rosario D'Agata, Saveria Aquila
Article
Oncology
Francesca Trotta, Paola Avena, Adele Chimento, Vittoria Rago, Arianna De Luca, Sara Sculco, Marta C. Nocito, Rocco Malivindi, Francesco Fallo, Raffaele Pezzani, Catia Pilon, Francesco M. Lasorsa, Simona N. Barile, Luigi Palmieri, Antonio M. Lerario, Vincenzo Pezzi, Ivan Casaburi, Rosa Sirianni
MOLECULAR CANCER THERAPEUTICS
(2020)
Article
Oncology
Luca Gelsomino, Giuseppina Daniela Naimo, Rocco Malivindi, Giuseppina Augimeri, Salvatore Panza, Cinzia Giordano, Ines Barone, Daniela Bonofiglio, Loredana Mauro, Stefania Catalano, Sebastiano Ando
Article
Biochemistry & Molecular Biology
Salvatore Panza, Umberto Russo, Francesca Giordano, Antonella Leggio, Ines Barone, Daniela Bonofiglio, Luca Gelsomino, Rocco Malivindi, Francesca Luisa Conforti, Giuseppina Daniela Naimo, Cinzia Giordano, Stefania Catalano, Sebastiano Ando
Article
Biology
Salvatore Panza, Francesca Giordano, Daniela De Rose, Maria Luisa Panno, Francesca De Amicis, Marta Santoro, Rocco Malivindi, Vittoria Rago, Saveria Aquila
Article
Oncology
Salvatore Panza, Rocco Malivindi, Amanda Caruso, Umberto Russo, Francesca Giordano, Balazs Gyorffy, Luca Gelsomino, Francesca De Amicis, Ines Barone, Francesca Luisa Conforti, Cinzia Giordano, Daniela Bonofiglio, Stefania Catalano, Sebastiano Ando
Summary: Losartan, as an adjuvant pharmacological tool, may represent a potential treatment for GBM by inhibiting local estrogen production and halting cell growth. The limited mortality benefit of current treatments underscores the importance of exploring new therapeutic avenues.
Article
Food Science & Technology
Umile Gianfranco Spizzirri, Paolino Caputo, Cesare Oliviero Rossi, Pasquale Crupi, Marilena Muraglia, Vittoria Rago, Rocco Malivindi, Maria Lisa Clodoveo, Donatella Restuccia, Francesca Aiello
Summary: Olive mill wastewater was used to synthesize an antioxidant macromolecule, which can be used as a functional ingredient in the preparation of functional puddings. The wastewater showed strong antioxidant properties and was successfully used to synthesize a polymeric conjugate with good toxicity characteristics and antioxidant performance. In comparison with non-functional counterparts, the wastewater exhibited better consistency and antioxidant features in the functional food.
Article
Nutrition & Dietetics
Sabrina Bossio, Anna Perri, Rocco Malivindi, Francesca Giordano, Vittoria Rago, Maria Mirabelli, Alessandro Salatino, Antonio Brunetti, Emanuela Alessandra Greco, Antonio Aversa
Summary: Recent studies have shown that oleuropein (OLE) found in olive leaf extract can inhibit tumor cell proliferation and reduce the invasiveness of cancer cells. It achieves this by modulating cell-cycle regulators, promoting apoptosis, and inhibiting cell migration and motility. However, further research is needed to understand the specific mechanisms involved, especially in seminoma cells.
Article
Multidisciplinary Sciences
Tiziana M. G. Pecora, Ortensia Ilaria Parisi, Walter Bertin, Barbara Ragazzo, Marco Dattilo, Norma Scigliano, Rocco Malivindi, Fabio Amone, Francesco Puoci
Summary: REF-FTP78, a class IIb medical device for the treatment of GERD, is developed based on polysaccharides from Aloe Barbadensis and fucoidans from brown seaweeds. It aims to protect the esophageal mucosa, promote wound healing, and exhibit antioxidant and anti-inflammatory properties. The study demonstrated significant barrier effect, wound healing promotion, and antioxidant/anti-inflammatory activities of REF-FTP78, supporting its use in GERD treatment.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Carmine Rocca, Anna De Bartolo, Maria Concetta Granieri, Vittoria Rago, Daniela Amelio, Flavia Falbo, Rocco Malivindi, Rosa Mazza, Maria Carmela Cerra, Loubna Boukhzar, Benjamin Lefranc, Jerome Leprince, Youssef Anouar, Tommaso Angelone
Summary: The cell-permeable PSELT provides pharmacological preconditioning cardioprotection by mitigating ERS and oxidative stress, and by regulating endogenous SELENOT.
Article
Pharmacology & Pharmacy
Sonia Trombino, Rocco Malivindi, Giuseppe Barbarossa, Roberta Sole, Federica Curcio, Roberta Cassano
Summary: Classical melanoma therapy often has side effects, affecting the efficacy and patient compliance. Drug delivery systems, such as solid lipid nanoparticles (SLNs), can improve drug stability, release kinetics, and enhance therapy safety and efficacy. In this study, SLNs were prepared using hydroquinone esterified with stearic acid and characterized using FT-IR and H-1-NMR. The efficacy of SLNs on COLO-38 human melanoma cells were evaluated, and their potential effects on apoptotic mechanisms were analyzed. Safety tests, cytotoxicity evaluation, and antioxidant and anti-inflammatory activities were also conducted.
Article
Biochemistry & Molecular Biology
Francesca Aiello, Rocco Malivindi, Marisa Francesca Motta, Pasquale Crupi, Rosa Nicoletti, Cinzia Benincasa, Maria Lisa Clodoveo, Vittoria Rago, Umile Gianfranco Spizzirri, Donatella Restuccia
Summary: Natural antioxidants extracted from waste derived from olive oil production have potential wound-healing properties, and can be used to produce biomedical conjugates for wound treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.