Versatility of subtilisin: A review on structure, characteristics, and applications

Engineering subtilisin proteases that specifically degrade active RAS

(2021) Yingwei Chen et al.   Communications Biology

Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

(2021) Revathi Gurunathan et al.   Scientific Reports

Protease propeptide structures, mechanisms of activation, and functions

(2020) Lise Boon et al.   CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY

Stroke Prevention With the PCSK9 (Proprotein Convertase Subtilisin-Kexin Type 9) Inhibitor Evolocumab Added to Statin in High-Risk Patients With Stable Atherosclerosis

(2020) Robert P. Giugliano et al.   STROKE

An alternative for proteinase K-heat-sensitive protease from fungus Onygena corvina for biotechnology: cloning, engineering, expression, characterization and special application for protein sequencing

(2020) Piotr M. Skowron et al.   Microbial Cell Factories

Homology modeling and heterologous expression of highly alkaline subtilisin-like serine protease from Bacillus halodurans C-125

(2020) Aşkın Tekin et al.   BIOTECHNOLOGY LETTERS

Extracellular Production, Characterization, and Engineering of a Polyextremotolerant Subtilisin-Like Protease From Feather-Degrading Thermoactinomyces vulgaris Strain CDF

(2020) Yidi Ding et al.   Frontiers in Microbiology

Unravelling protein -organic solvent interaction of organic solvent tolerant elastase from Pseudomonas aeruginosa strain K crystal structure

(2019) Zatty Syamimi @. Adura Mat Said et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Changes in circulating pro-protein convertase subtilisin/kexin type 9 levels – experimental and clinical approaches with lipid-lowering agents

(2019) C Macchi et al.   European Journal of Preventive Cardiology

Insight into subtilisin E-S7 cleavage pattern based on crystal structure and hydrolysates peptide analysis

(2019) Heng Tang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Improvement of the acid resistance, catalytic efficiency, and thermostability of nattokinase by multisite‐directed mutagenesis

(2019) Zhongmei Liu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Purification and biochemical characterization of a novel thermostable and halotolerant subtilisin SAPN, a serine protease from Melghiribacillus thermohalophilus Nari2AT for chitin extraction from crab and shrimp shell by-products

(2019) Sondes Mechri et al.   EXTREMOPHILES

Pharmaceutically modified subtilisins withstand acidic conditions and effectively degrade gluten in vivo

(2019) Ghassan Darwish et al.   Scientific Reports

Calcium ion induced thermodynamic stability, bisubstrate specificity, and differential organic solvent tolerance of a predominantly β‐sheet serine protease from Bacillus aquimaris VITP4

(2019) Chittoor Jabeena Thaz et al.   BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

Identification of a novel protease from the thermophilic Anoxybacillus kamchatkensis M1V and its application as laundry detergent additive

(2019) Sondes Mechri et al.   EXTREMOPHILES

Facile synthesis of alcalase-inorganic hybrid nanoflowers used for soy protein isolate hydrolysis to improve its functional properties

(2019) Amjad Hussain Memon et al.   FOOD CHEMISTRY

Improving the catalytic performance of Proteinase K from Parengyodontium album for use in feather degradation

(2019) Yaxin Ren et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

A General Overview of Support Materials for Enzyme Immobilization: Characteristics, Properties, Practical Utility

(2018) Jakub Zdarta et al.   Catalysts

Molecular dynamics and structure function analysis show that substrate binding and specificity are major forces in the functional diversification of Eqolisins

(2018) Nicolás Stocchi et al.   BMC BIOINFORMATICS

Insights into the role of electrostatics in temperature adaptation: a comparative study of psychrophilic, mesophilic, and thermophilic subtilisin-like serine proteases

(2018) Yuan-Ling Xia et al.   RSC Advances

Design of antimicrobial polycaprolactam nanocomposite by immobilizing subtilisin conjugated Au/Ag core-shell nanoparticles for biomedical applications

(2018) Veluchamy Prabhawathi et al.   Materials Science & Engineering C-Materials for Biological Applications

Improved expression, purification and characterization of VPR, a cold active subtilisin-like serine proteinase and the effects of calcium on expression and stability

(2018) Kristinn R. Óskarsson et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Molecular Modeling of Cloned Bacillus subtilis Keratinase and Its Insinuation in Psoriasis Treatment Using Docking Studies

(2017) Sonali Gupta et al.   INDIAN JOURNAL OF MICROBIOLOGY

Optimized production and characterization of a detergent-stable protease from Lysinibacillus fusiformis C250R

(2017) Sondes Mechri et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Improving the Efficiency of New Automatic Dishwashing Detergent Formulation by Addition of Thermostable Lipase, Protease and Amylase

(2017) Ashwini Naganthran et al.   MOLECULES

The MEROPS database of proteolytic enzymes, their substrates and inhibitors in 2017 and a comparison with peptidases in the PANTHER database

(2017) Neil D Rawlings et al.   NUCLEIC ACIDS RESEARCH

Identification of food-grade subtilisins as gluten-degrading enzymes to treat celiac disease

(2016) Guoxian Wei et al.   AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY

Purification and characterization of pepsinogen and pepsin from the stomach of rainbow trout (Oncorhynchus mykiss)

(2016) Maleen Wald et al.   EUROPEAN FOOD RESEARCH AND TECHNOLOGY

Production of ACE inhibitory peptides from sweet sorghum grain protein using alcalase: Hydrolysis kinetic, purification and molecular docking study

(2016) Qiongying Wu et al.   FOOD CHEMISTRY

Functional, bioactive and antigenicity properties of blue whiting protein hydrolysates: effect of enzymatic treatment and degree of hydrolysis

(2016) Pedro J García-Moreno et al.   JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE

Purification and characterization of naturally occurring HIV-1 (South African subtype C) protease mutants from inclusion bodies

(2016) Sibusiso B. Maseko et al.   PROTEIN EXPRESSION AND PURIFICATION

Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): A review

(2016) Imen Hamed et al.   TRENDS IN FOOD SCIENCE & TECHNOLOGY

Improving the activity of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulation

(2015) Meizhi Weng et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Purification and characterisation of κ-casein specific milk-clotting metalloprotease from Termitomyces clypeatus MTCC 5091

(2015) Rajib Majumder et al.   FOOD CHEMISTRY

Characterization of a new, recombinant thermo-active subtilisin-like serine protease derived from Shewanella arctica

(2015) Farah Qoura et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Simultaneous release of peptides and phenolics with antioxidant, ACE-inhibitory and anti-inflammatory activities from pinto bean (Phaseolus vulgaris L. var. pinto) proteins by subtilisins

(2015) Patricia Garcia-Mora et al.   Journal of Functional Foods

Secretory expression of thermostable alkaline protease from Bacillus stearothermophilus FI by using native signal peptide and α-factor secretion signal in Pichia pastoris

(2015) Amaliawati Ahmad Latiffi et al.   GENES & GENETIC SYSTEMS

Four residues of propeptide are essential for precursor folding of nattokinase

(2014) Yan Jia et al.   ACTA BIOCHIMICA ET BIOPHYSICA SINICA

Nattokinase: production and application

(2014) Fatemeh Dabbagh et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

YASARA View—molecular graphics for all devices—from smartphones to workstations

(2014) Elmar Krieger et al.   BIOINFORMATICS

Formation of the High-Affinity Calcium Binding Site in Pro-subtilisin E with the Insertion Sequence IS1 of Pro-Tk-subtilisin

(2013) Ryo Uehara et al.   BIOCHEMISTRY

Enzymatic activity of a subtilisin homolog, Tk-SP, from Thermococcus kodakarensis in detergents and its ability to degrade the abnormal prion protein

(2013) Azumi Hirata et al.   BMC BIOTECHNOLOGY

Efficient expression and purification of recombinant human μ-calpain using anEscherichia coliexpression system

(2013) Shoji Hata et al.   GENES TO CELLS

Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg

(2013) Dragana Despotovic et al.   JOURNAL OF BIOTECHNOLOGY

Entrapment of Subtilisin in Ceramic Sol–Gel Coating for Antifouling Applications

(2012) Viduthalai Rasheedkhan Regina et al.   ACS Applied Materials & Interfaces

Requirement of Ca2+ Ions for the Hyperthermostability of Tk-Subtilisin from Thermococcus kodakarensis

(2012) Ryo Uehara et al.   BIOCHEMISTRY

Characteristics and applications of a recombinant alkaline serine protease from a novel bacterium Bacillus lehensis

(2012) Swati Joshi et al.   BIORESOURCE TECHNOLOGY

Directed Evolution of Subtilisin E into a Highly Active and Guanidinium Chloride- and Sodium Dodecylsulfate-Tolerant Protease

(2012) Zhenwei Li et al.   CHEMBIOCHEM

Accelerated maturation of Tk-subtilisin by a Leu→Promutation at the C-terminus of the propeptide, which reduces the binding of the propeptide to Tk-subtilisin

(2012) Ryo Uehara et al.   FEBS Journal

The crystal structure of an intermediate dimer of aspergilloglutamic peptidase that mimics the enzyme-activation product complex produced upon autoproteolysis

(2012) H. Sasaki et al.   JOURNAL OF BIOCHEMISTRY

Effect of Ion Pair on Thermostability of F1 Protease: Integration of Computational and Experimental Approaches

(2012) Raja Noor Zaliha Raja Abd Rahman   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Efficient enzyme-catalyzed synthesis of peptide secondary amides for use as serine proteinase inhibitors

(2012) Maxim E. Sergeev et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Angiotensin I converting enzyme (ACE) inhibitory peptides from salmon byproduct protein hydrolysate by Alcalase hydrolysis

(2012) Chang-Bum Ahn et al.   PROCESS BIOCHEMISTRY

Influence of Enzymatic Hydrolysis on the Allergenicity of Roasted Peanut Protein Extract

(2011) Beatriz Cabanillas et al.   INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY

Immobilization of Subtilisin on Polycaprolactam for Antimicrobial Food Packaging Applications

(2011) Prabhawathi Veluchamy et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

Cloning of a Fibrinolytic Enzyme (Subtilisin) Gene From Bacillus subtilis in Escherichia coli

(2011) Younes Ghasemi et al.   MOLECULAR BIOTECHNOLOGY

Purification, crystallization and preliminary X-ray diffraction experiment of nattokinase fromBacillus subtilis natto

(2010) Yasuhide Yanagisawa et al.   Acta Crystallographica Section F-Structural Biology and Crystallization Communications

Crystal structure of an intramolecular chaperone mediating triple–β-helix folding

(2010) Eike C Schulz et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Crystal Structure of an Intracellular Subtilisin Reveals Novel Structural Features Unique to this Subtilisin Family

(2010) Jitka Vévodová et al.   STRUCTURE

Angiotensin Converting Enzyme (ACE) Inhibitory Peptides: Production and Implementation of Functional Food

(2009) F. De Leo et al.   CURRENT PHARMACEUTICAL DESIGN

Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand

(2009) Randa A Abusham et al.   Microbial Cell Factories

Stability of thermostable alkaline protease from Bacillus licheniformis RP1 in commercial solid laundry detergent formulations

(2006) Alya Sellami-Kamoun et al.   MICROBIOLOGICAL RESEARCH