CRISPR/Cas12a collateral cleavage activity for simple and rapid detection of protein/small molecule interaction

An ultrasensitive and point-of-care strategy for enzymes activity detection based on enzyme extends activators to unlock the ssDNase activity of CRISPR/Cas12a (EdU-CRISPR/Cas12a)

(2021) Xiaolong Chen et al.   SENSORS AND ACTUATORS B-CHEMICAL

Identification of thyroid hormone/thyroid hormone receptor interaction based on aptamer-assisted protein-induced fluorescence enhancement

(2021) Jayeon Song et al.   BIOSENSORS & BIOELECTRONICS

CRISPR-Cas12a based aptasensor for sensitive and selective ATP detection

(2020) Lei Peng et al.   SENSORS AND ACTUATORS B-CHEMICAL

G-triplex molecular beacon‒based fluorescence biosensor for sensitive detection of small molecule-protein interaction via exonuclease III‒assisted recycling amplification

(2020) Qiang Liu et al.   SENSORS AND ACTUATORS B-CHEMICAL

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

Colorimetric Detection of SARS-CoV-2 and Drug-Resistant pH1N1 Using CRISPR/dCas9

(2020) Jeong Moon et al.   ACS Sensors

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Rolling Circular Amplification (RCA)-Assisted CRISPR/Cas9 Cleavage (RACE) for Highly Specific Detection of Multiple Extracellular Vesicle MicroRNAs

(2019) Ruixuan Wang et al.   ANALYTICAL CHEMISTRY

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

(2019) Ying Xiong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A simple and sensitive detection of small molecule–protein interactions based on terminal protection-mediated exponential strand displacement amplification

(2018) Chang Yeol Lee et al.   ANALYST

Small molecule–protein interactions in branch migration thermodynamics: modelling and application in the homogeneous detection of proteins and small molecules

(2018) Xing Zhou et al.   ANALYST

Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 Triggered Isothermal Amplification for Site-Specific Nucleic Acid Detection

(2018) Mengqi Huang et al.   ANALYTICAL CHEMISTRY

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

Chemical modifications and reactions in DNA nanostructures

(2017) Kurt V. Gothelf   MRS BULLETIN

Binding-regulated click ligation for selective detection of proteins

(2016) Ya Cao et al.   BIOSENSORS & BIOELECTRONICS

Detection of biotin-streptavidin interactions via terminal protection of small molecule linked DNA and the formation of fluorescent DNA-templated silver nanoclusters

(2016) Yue He et al.   MICROCHIMICA ACTA

A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA

(2015) Hai-Bo Wang et al.   BIOSENSORS & BIOELECTRONICS

Development and Applications of CRISPR-Cas9 for Genome Engineering

(2014) Patrick D. Hsu et al.   CELL

Photoaffinity Labeling of Small-Molecule-Binding Proteins by DNA-Templated Chemistry

(2013) Gang Li et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Methods for the Elucidation of Protein-Small Molecule Interactions

(2013) Amanda McFedries et al.   CHEMISTRY & BIOLOGY

ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering

(2013) Thomas Gaj et al.   TRENDS IN BIOTECHNOLOGY

Systematic investigation of protein-small molecule interactions

(2012) Xiyan Li et al.   IUBMB LIFE

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

Selection of Smart Small-Molecule Ligands: The Proof of Principle

(2008) Andrei P. Drabovich et al.   ANALYTICAL CHEMISTRY

Protein networks in disease

(2008) T. Ideker et al.   GENOME RESEARCH

DNA-Small Molecule Chimera with Responsive Protein-Binding Ability

(2008) D. Calvin Harris et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY