Review
Biotechnology & Applied Microbiology
A. B. Cardillo, M. Perassolo, A. M. Giulietti, Julian Rodriguez Talou
Summary: Cyclodextrins trigger secondary metabolite production and release in plant cell cultures, forming complexes with bioactive compounds to reduce feedback inhibition and degradation. Plant cell culture is an alternative source for continuous and homogeneous production of secondary metabolites, overcoming intermittent supply from natural or agricultural sources. Elicitation with cyclodextrins can boost plant cell defense responses and accumulation of secondary metabolites, contributing to product removal and preventing degradation and toxicity.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Biochemistry & Molecular Biology
Nisar Ahmad, Palwasha Khan, Abdullah Khan, Maliha Usman, Mohammad Ali, Hina Fazal, Durrishahwar, Muhammad Nazir Uddin, Christophe Hano, Bilal Haider Abbasi
Summary: This study investigated the effect of Cuscuta reflexa extract as a biotic elicitor on the biomass accumulation and production of antioxidant secondary metabolites in submerged adventitious root cultures of Stevia rebaudiana. The results showed that Cuscuta reflexa extract moderately improved biomass accumulation and enhanced the synthesis of phenolics, flavonoids, and antioxidant activities.
Article
Plant Sciences
Gregorio Barba-Espin, Christian Martinez-Jimenez, Alberto Izquierdo-Martinez, Jose R. Acosta-Motos, Jose A. Hernandez, Pedro Diaz-Vivancos
Summary: Hairy roots grown in vitro, particularly from black carrot, were used in this study for their ability to accumulate anthocyanin. By modulating growth medium composition and H2O2 elicitation, anthocyanin accumulation and biomass production were enhanced. Elicitation with H2O2 led to increased superoxide dismutase activity and anthocyanin accumulation in the hairy roots.
Article
Biochemistry & Molecular Biology
Romeo Arago Dougue Kentsop, Roberto Consonni, Michela Alfieri, Marina Laura, Gianluca Ottolina, Iride Mascheretti, Monica Mattana
Summary: This study aims to develop adventitious and hairy roots from Linum lewisi and evaluate their suitability for large-scale production of lignans, including justicidin B. The results indicate that these cultures have higher justicidin B production compared to other Linum species cultures, and the production can be further increased with elicitation.
Review
Plant Sciences
Diptesh Biswas, Avijit Chakraborty, Swapna Mukherjee, Biswajit Ghosh
Summary: This study highlights the importance of hairy roots in the production of secondary metabolites in Solanaceous plants. It provides an overview of modern biotechnological techniques used to enhance the production of these metabolites in hairy root cultures.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Mengxi Wu, Trent R. Northen, Yezhang Ding
Summary: Plants produce specialized metabolites that play crucial roles in environmental interactions and stress adaptation. These compounds have significant agricultural, medicinal, and industrial values. Recent advances in genomics, transcriptomics, metabolomics, proteomics, and computational approaches have made the exploration of plant specialized metabolism more feasible. These advancements hold promise for enhancing plant stress resilience through harnessing the potential of specialized metabolites.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Ramaraj Sathasivam, Minsol Choi, Ramalingam Radhakrishnan, Haejin Kwon, Jiwon Yoon, So Hwi Yang, Jae Kwang Kim, Yong Suk Chung, Sang Un Park
Summary: The hairy root (HR) culture system is an excellent alternative strategy for mass production of valuable compounds. Selection of suitable Agrobacterium strain is essential for enhanced production of beneficial secondary metabolites. In this study, Agrobacterium strain R1601 was found to be one of the most promising strains for inducing mass HR production and enhanced levels of secondary metabolites in Ocimum basilicum.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Masooma Jawad Hussain, Yawar Abbas, Naushaba Nazli, Sara Fatima, Samantha Drouet, Christophe Hano, Bilal Haider Abbasi
Summary: Medicinal plants are crucial for pharmaceutical drugs, but their existence is threatened by overexploitation. Adventitious root and hairy root culture systems provide an alternative method for producing valuable compounds efficiently. This review summarizes the recent global research on these methods and compares their effectiveness. Factors such as culture conditions and inducer concentrations are also discussed.
Article
Biochemical Research Methods
Yun Ji Park, Jae Kwang Kim, Sang Un Park
Summary: The dried root of Astragalus membranaceus is a well-known herbal medicine with beneficial effects on chronic diseases and weakness. Astragalosides, natural compounds found in Astragalus membranaceus, are used in treating diabetes and cardiovascular diseases. This study demonstrates that adding yeast extract can effectively increase astragaloside content in the roots, providing valuable information for improving astragaloside industrial production.
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Jiao Jiao, Lan Yao, Jin-Xian Fu, Yao Lu, Qing-Yan Gai, Xue Feng, Xiao-Jia He, Run-Ze Cao, Yu-Jie Fu
Summary: Pigeon pea hairy root cultures (PPHRCs) were cocultured with Aspergillus oryzae CGMCC 3.951 (AO 3.951) to enhance the production of cajaninstilbene acid (CSA), a health-promoting compound. AO 3.951 elicitation led to a significant increase in CSA content in both hairy roots and culture media, while reducing flavonoid glycosides. AO 3.951 also activated the expression of key enzyme genes involved in CSA biosynthesis and transporter genes associated with CSA transmembrane transport. The cocultivation approach holds great potential for commercial production of high-value phenolic compounds like CSA.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Agricultural Engineering
Ganesan Mahendran, Narsingh Verma, Manju Singh, Karuna Shanker, Suchitra Banerjee, Birendra Kumar, Laiq ur Rahman
Summary: This study aimed to design an efficient hairy root induction protocol for Swertia chirayita and investigate the effects of methyl jasmonate, sodium nitroprusside, and salicylic acid on the production of bioactive xanthones. The results showed that the highest root induction frequency and biomass were achieved with specific cell density, infection time, and acetosyringone concentration. Furthermore, the content of bioactive xanthones was significantly increased with the application of methyl jasmonate. Overall, the developed hairy root protocol of Swertia chirayita is cost-effective and suitable for large-scale industrial production of bioactive xanthones.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Hanhan Qiu, Lingye Su, Hongfeng Wang, Zongshen Zhang
Summary: The study demonstrates that chitosan can enhance triterpenoid saponin biosynthesis in Psammosilene tunicoides hairy roots by promoting antioxidant activities, nitric oxide production, and differential gene expression.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Ewa Kochan, Grazyna Szymanska, Pawel Kwiatkowski, Monika Sienkiewicz
Summary: Ginsenosides, triterpenoid saponins found in the roots of Panax qiunquefolius, have various pharmacological properties. This study showed that using linalool as an elicitor can increase the production of ginsenosides in hairy root cultures of P. quinquefolius without relying on field-grown plants and the vegetative season. Elicitation with linalool can be an effective method of improving ginseng saponin production in P. quinquefolium hairy root cultures.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Review
Plant Sciences
Kevin J. Morey, Christie A. M. Peebles
Summary: While plants are a valuable source of natural products, their commercial application faces challenges. Hairy root cultures provide a potential production platform for non-agricultural plants, with advancements in metabolic engineering, synthetic biology tools, and bioreactor technology enabling commercial application.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Agata Rogowska, Malgorzata Stpiczynska, Cezary Paczkowski, Anna Szakiel
Summary: The interplay between steroids and triterpenoids, compounds with distinct functions but sharing the same biosynthetic pathway, is crucial for plant defense. Exogenous application of jasmonic acid can significantly enhance triterpenoid saponin production, and different plant organs may respond differently to jasmonic acid.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Neha Tanwar, Sagar S. Arya, James E. Rookes, David M. Cahill, Sangram K. Lenka, Kailash C. Bansal
Summary: Chloroplast genome engineering is a promising approach for enhancing the nutritional value of food crops by increasing the levels of existing metabolites, restoring lost metabolites during crop domestication, and introducing novel nutritional compounds.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Nandini Bhattacharya, David M. Cahill, Wenrong Yang, Mandira Kochar
Summary: Graphene, with its unique properties, has great potential as a nano-delivery system in both biomedicine and agriculture. Its large surface area, chemical and mechanical stability, tunable surface chemistry and low toxicity make it a promising material for agricultural delivery systems. Recent research has shown success in using graphene as a delivery vehicle for plant nutrients and crop protection agents in agriculture.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Chemistry, Analytical
Sagar S. Arya, Nada K. Morsy, Deema K. Islayem, Sarah A. Alkhatib, Charalampos Pitsalidis, Anna-Maria Pappa
Summary: Plasma membrane mimetics have the potential to be important in drug discovery and immunotherapy by allowing the assembly of cellular membranes on surfaces and/or nanoparticles for direct assessment of drug/membrane interactions. Bacterial membranes (BMs) have been widely used in biomedical research due to the rise of antibiotic resistance and bacterial infections as major causes of death. Advancements in nanotechnology and bioelectronics have greatly enhanced BM research, resulting in multifaceted systems for various applications in sensing and drug discovery. This review discusses the use of BMs in biosensing, bioimaging, drug delivery/discovery, and immunotherapy, with a focus on bacterial infections and cancer.
Article
Development Studies
Muneer Ahmad Magry, David Cahill, James Rookes, Sapna A. Narula
Summary: Our study investigates the impact of climate change on non-timber forest products in Khunti district, India. Through questionnaires, interviews, and data analysis, we found that changing climate has significantly decreased the yield and quality of these products, leading to lower sale prices and affecting livelihoods. Respondents also identified infrastructure development and forest cover loss as key factors contributing to these climate change impacts.
CLIMATE AND DEVELOPMENT
(2023)
Article
Plant Sciences
Prafulla K. Behera, Vajinder Kumar, Shyam S. Sharma, Sangram K. Lenka, Debabrata Panda
Summary: This study evaluated the physiological responses and genetic diversity of selected short-grain aromatic rice landraces to multiple abiotic stresses. Molecular marker analysis revealed high polymorphism in 96 loci and genetic dissimilarity among the rice genotypes. Certain aromatic genotypes were found to be genetically close to drought-tolerant and flooding-tolerant cultivars, while salt-tolerant varieties showed similarity to specific cultivars. The obtained genetic variants have implications for landrace conservation, selection, and global abiotic stress tolerance.
CURRENT PLANT BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Neha Tanwar, James E. Rookes, David M. Cahill, Sangram K. Lenka
Summary: The carotenoid pathway in plants has been engineered to produce keto-carotenoids, which are highly valued in the food, feed, and human health industries. This study successfully manipulated the native carotenoid pathway in tobacco plants through chloroplast engineering to produce keto-lutein. By introducing a ketolase gene along with lycopene cyclase and hydroxylase genes, the carotenoid pathway was redirected towards the xanthophyll cycle and resulted in the production of keto-lutein. This scalable molecular genetic platform provides a means for producing novel keto-carotenoids in tobacco using the DBTL approach.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Plant Sciences
Awadhesh Kumar, Goutam Kumar Dash, Soumya Kumar Sahoo, Milan Kumar Lal, Upasana Sahoo, Rameswar Prasad Sah, Umakanta Ngangkham, Sudhir Kumar, Mirza Jaynul Baig, Srigopal Sharma, Sangram Keshari Lenka
Summary: In cereals and legumes, phytic acid (PA) serves as a phosphorus reserve for growth and has both antioxidant activity and ability to bind minerals. Although it is considered an anti-nutritional factor due to its reduced bioavailability of minerals and proteins, PA has various health benefits including lowering the risk of diabetes and some cancers, improving heart health, and preventing kidney stone formation. Additionally, it has been found to be effective against foodborne bacteria. This paper provides an overview of phosphorus transport in plants, PA biosynthesis, its beneficial and anti-nutritional effects, and dephytinization methods.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Agriculture, Multidisciplinary
Ananya Padmakumar, Chowdary Pavani, Kalyani Eswar, Lingxue Kong, Wenrong Yang, Subramanium Gopalakrishnan, David Miles Cahill, Aravind Kumar Rengan
Summary: The use of bacterial biocontrol agents (BBCAs) in plant protection has increased due to the rising awareness of chemical risks and the need for sustainable agriculture. Nanotechnology has facilitated the development of efficient and ecologically sound nanoformulations, such as bacteria premised nanobiopesticides (B-NBPs). These B-NBPs are synthesized using nanomaterials with amended properties at the nanoscale. This article provides an overview of BBCAs and their antagonistic mechanisms, focusing on the significance of BNPs in plant infection management and addressing concerns related to the efficacy of B-NBPs.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2023)
Article
Plant Sciences
Thi Linh Chi Tran, Albert Guirguis, Thanojan Jeyachandran, Yichao Wang, David M. Cahill
Summary: Nanoparticles have been shown to enhance plant growth and development, and improve crop production, especially under adverse environmental conditions like drought. Silicon dioxide nanoparticles have been found to be taken up by plants and have the potential to improve tolerance to abiotic stress.
FUNCTIONAL PLANT BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Anamika Pal, Pawan Kaur, Neeraj Dwivedi, Jim Rookes, Himadri B. Bohidar, Wenrong Yang, David Miles Cahill, Palash Kumar Manna
Summary: Clay-nanocomposite materials have been widely used for drug delivery systems, but their application in agriculture is less explored. The combination of nanoclay and active ingredients can create a biocompatible and environmentally friendly delivery vehicle for micronutrients, pesticides, etc., improving crop growth. This review provides an overview of the synthesis and application of clay-based nanocomposites in agriculture, addressing issues related to the delivery of active ingredients and biological macromolecules for crop protection and sustainability.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2023)
Review
Agriculture, Multidisciplinary
Bhaskar Sharma, Udit Soni, Luis O. B. Afonso, David M. Cahill
Summary: Nanotechnology has the potential to revolutionize agriculture through the use of nanomaterials and the concept of doping to enhance their properties. However, further research and investment are needed to fully explore and develop these advanced technologies for smart farming.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)
