4.5 Article

N-efficient cotton genotype grown under low nitrogen shows relatively large root system, high biomass accumulation and nitrogen metabolism

Journal

AGRONOMY JOURNAL
Volume 114, Issue 1, Pages 582-600

Publisher

WILEY
DOI: 10.1002/agj2.20962

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Funding

  1. Cotton Research Institute, Chinese Academy of Agricultural Sciences, Central Level Public Welfare Scientific Institutes Basic Research and Business Special Funding Project [1610162021025]
  2. National Key Research and Development Program of China [2017YFD0101600]
  3. State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS [CB2021C10]

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This study investigated how cotton plants balance nitrogen (N) metabolism and enhanced biomass accumulation to support larger root systems under low N stress. The results showed that high N-efficient genotype CCRI-69 produced a larger root system, high biomass accumulation, and efficient N metabolism compared to N-inefficient genotype XLZ-30. The key traits contributing to N use efficiency (NUE) were root morphology, sugar contents, and N-assimilating enzymatic activities.
A larger root system enhanced biomass accumulation, and high nitrogen (N) metabolism is an effective mechanism of plants in response to low N stress. How cotton plants balance N metabolism and enhanced biomass accumulation to support larger root systems under low N stress remains to be elucidated. This study deals with the changes in root development due to biomass accumulation and N metabolism in contrasting N-efficient cotton genotypes (CCRI-69, N-efficient, and XLZ-30, N-inefficient) under low (0.2 mM) and normal (2 mM) N concentrations. The results showed that genotype CCRI-69 produced a larger root system, high biomass accumulation, and N metabolism than XLZ-30. The flourishing root system of CCRI-69 was due to more allocation of sugars from shoot to root, especially under low N concentrations, to support efficient N uptake followed by high N metabolism and N use efficiency (NUE). In addition, CCRI-69 produced more dry biomass, high photosynthetic activity, N-related enzymatic activities, N uptake, and utilization under normal N concentration compared with XLZ-30. Based on multivariate analysis, root morphological traits, sugar contents, and N-assimilating enzymatic activities might be considered the key traits contributing to NUE and can also be used as potential indicators for genotypic evaluation. In the future, high N metabolism and allocation of more sugars under low N concentration could be studied at a molecular level to better understand the underlying molecular mechanism of NUE in cotton.

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