Article
Cell Biology
Mako Sasao, Tomohide Uno, Risa Kitagawa, Asuka Matsui, Fumika Toryu, Akira Mizoguchi, Kengo Kanamaru, Katsuhiko Sakamoto, Yuichi Uno
Summary: SNAREs play a core role in mediating membrane fusion, and Bombyx mori SNAP-25, VAMP2, and Syntaxin 1A are involved in specific regions of the brain in relation to membrane fusion. These proteins are also present in Bombyx mori's prothoracicotropic hormone (PTTH)-secretory neurons and overlap with the neurospecific protein Bombyxin.
HISTOCHEMISTRY AND CELL BIOLOGY
(2023)
Article
Entomology
Tomohide Uno, Yusuke Ozakiya, Mako Sasao, Katsuhiko Sakamoto, Yasuo Yamauchi, Yuichi Uno, Kengo Kanamaru, Akira Mizoguchi
Summary: The study found that Rab3, 7, 39, and X4 may regulate the exocytosis of bombyxin in the brain of Bombyx mori, showing a specific distribution pattern in the insect.
EUROPEAN JOURNAL OF ENTOMOLOGY
(2021)
Article
Genetics & Heredity
Hai-Xu Bian, Dong-Bin Chen, Yu-Ping Li, En-Guang Tan, Xin Su, Jing-Chao Huang, Jun-Fang Su, Yan-Qun Liu
Summary: This study reports the availability of the transcriptome resource of the isolated corpus allatum from the final instar larvae of the silkworm when the juvenile hormone (JH) titer is low, and compares it with the prothoracic gland to identify common features in JH and 20E related genes. The results show that certain genes related to JH biosynthesis and regulation as well as 20E are expressed in both organs, suggesting potential cross talk between the corpus allatum and prothoracic gland.
Article
Biochemistry & Molecular Biology
Jimena Leyria, Samiha Benrabaa, Marcela Nouzova, Fernando G. G. Noriega, Lilian Valadares Tose, Francisco Fernandez-Lima, Ian Orchard, Angela B. B. Lange
Summary: This study used the Rhodnius prolixus model to investigate the importance of nutrition, insulin signaling, and two main types of insect lipophilic hormones, juvenile hormone (JH) and ecdysteroids, in successful reproduction. The results showed that insulin and allatoregulatory neuropeptides mainly drive the increase in JH levels after a blood meal, and JH in turn regulates its own biosynthesis by controlling the expression of key enzymes. Additionally, insulin stimulates the synthesis and release of ecdysteroids from the ovary. This study emphasizes the complexity of the endocrine signal network and how they coordinate a successful reproductive cycle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Entomology
Yan-Xia Sun, Bang-Xian Zhang, Fang-Fang Liu, Xiang-Jun Rao
Summary: This study found that the CTL5 gene is mainly expressed in fourth-instar larvae, adult moths, and the larval epidermis of Bombyx mori. CTL5 expression responds differently to pathogenic stimuli and the molting hormone 20-hydroxyecdysone. Recombinant proteins can bind to hemocytes, polysaccharides, bacteria, and spores, and interact with integrin β2-β5 subunits. Injection of recombinant CTL5 stimulates the expression of immune genes and enhances the clearance of pathogens.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Multidisciplinary Sciences
Ivana Daubnerova, Ladislav Roller, Honoo Satake, Chen Zhang, Young-Joon Kim, Dusan Zitnan
Summary: Insect ecdysis triggering hormones act on specific neurons in the central nervous system to activate the ecdysis sequence, with different ETH receptor subtypes showing distinct spatial and temporal expression patterns in the CNS to control various stages of the ecdysis process.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Jing Xu, Xiaole Xie, Qiuqin Ma, Lu Zhang, Yu Li, Yin Chen, Kang Li, Yang Xiao, Gianluca Tettamanti, Hanhong Xu, Ling Tian
Summary: This study characterized the molecules involved in Bombyx mori nucleopolyhedrovirus (BmNPV)-silkworm interaction by RNA sequencing. The upregulated genes were mainly involved in translation, signal transduction, folding, sorting, and degradation, while the downregulated genes were predominantly related to metabolism. Knockout of specific genes inhibited or promoted BmNPV proliferation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Food Science & Technology
Emily N. Brogan, Yong-Lak Park, Kristen E. Matak, Jacek Jaczynski
Summary: The study on cricket, locust, and silk worm pupae powders revealed that these insect powders are rich in protein and have a diverse amino acid composition, but may not be suitable for infants. Protein from insects can be efficiently isolated using pH-solubility-precipitation method.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jiamin Yan, Zhao Xue, Haonan Dong, Jiaxin Pang, Huawei Liu, Jing Gong, Qingyou Xia, Yong Hou
Summary: In this study, the expression and regulation pathways of storage protein genes in silkworms were investigated. The results showed that the expression of storage proteins in silkworm larvae was strongly decreased by starvation, and the insulin-like signaling pathway played an important role in the regulation of storage protein expression. The ILS/FoxO signaling pathway responds to nutrient uptake by regulating the expression of storage proteins.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhihua Hao, Qingyu Lu, Yanyan Zhou, Yanting Liang, Yun Gao, Huanyan Ma, Yusong Xu, Huabing Wang
Summary: The use of pesticides can weaken the immune system of non-target organisms, and there is a lack of common biomarkers to detect these effects. This study found that MyD88 could potentially serve as a biomarker for pesticide-induced stress in Bombyx mori.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Feifei Ren, Jiming Yan, Xiong Wang, Yukai Xie, Nan Guo, Luc Swevers, Jingchen Sun
Summary: In this study, RNA-seq analysis of the silkworm midgut after BmCPV1 infection identified 2520 differentially expressed genes (DEGs). Gene Ontology (GO) functional annotation showed that the DEGs predominantly functioned in binding, cell, and cellular processes. Kyoto Encyclopedia of Genes and Genomes (KEGG) functional annotation revealed that the DEGs were mainly distributed in global and overview metabolism maps, translation, and signal transduction. Experimental validation confirmed that BmPGRP-S5 acts as a proviral host factor for BmCPV1 infection.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Entomology
Gabriela-Maria Baci, Alexandra-Antonia Cucu, Alexandru-Ioan Giurgiu, Adriana-Sebastiana Musca, Lilla Bagameri, Adela Ramona Moise, Otilia Bobis, Attila Cristian Ratiu, Daniel Severus Dezmirean
Summary: CRISPR-Cas is a powerful gene editing tool that has made remarkable progress in B. mori genome editing, advancing research in the fields of entomology and biological sciences.
Article
Biochemistry & Molecular Biology
Zhengwen Yan, Xiaoling Tong, Gao Xiong, Weike Yang, Kunpeng Lu, Yajie Yuan, Minjin Han, Hai Hu, Wei Wei, Fangyin Dai
Summary: Insect cuticle plays a critical role in the adaptability of insects to the environment and their resistance to insecticides. This study focused on the structure and protein components of the cuticle at each developmental stage of the silkworm (Bombyx mori). The researchers found that the processes of cuticle formation were similar across the larval, pupal, and adult stages, and a large portion of the expressed cuticular protein (CP) genes were shared among these stages. However, there were also stage-specific CP genes expressed in each cuticle. The types and levels of CP genes may be important factors determining the properties of each cuticular layer. This study provides a comprehensive understanding of cuticle morphology and protein components, and contributes to future research on the molecular mechanisms underlying cuticle formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Xiancui Zhang, Huihui Feng, Jintao He, Abrar Muhammad, Fan Zhang, Xingmeng Lu
Summary: The study identified an Enterococcus species that is stably associated with the larval gut of the model organism B. mori, and investigated the colonization mechanism of this bacteria in the intestine. The results revealed the colonization properties and genomic features of the Enterococcus species, and showed its effectiveness against other gut bacteria. Additionally, successful colonization led to significant upregulation of genes related to adhesion, defense, regulation, secretion, and immune evasion.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Entomology
Peng Chen, Ling Wang, Yan-Bi Long, Guang-Yan Liang, Xiu Yang, Zhan-Qi Dong, Xia Jiang, Yan Zhu, Min-Hui Pan, Cheng Lu
Summary: E2F4 plays a critical role in regulating the cell cycle in the silkworm, Bombyx mori. It can localize in the nucleus through interaction with the Bm14-3-3 zeta protein. These findings provide valuable insights into the function of E2F transcription factors and their involvement in cell cycle regulation.
Article
Pharmacology & Pharmacy
Tomohide Uno, Chika Ogura, Chiho Izumi, Masahiko Nakamura, Takeshi Yanase, Hiroshi Yamazaki, Hitoshi Ashida, Kengo Kanamaru, Hiroshi Yamagata, Hiromasa Imaishi
BIOPHARMACEUTICS & DRUG DISPOSITION
(2015)
Article
Environmental Sciences
Tomohide Uno, Chiho Izumi, Shinji Takenaka, Takeshi Yanase, Hiromasa Imaishi, Kengo Kanamaru, Hiroshi Yamagata, Yoshio Kaminishi, Takao Itakura
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2015)
Article
Biochemistry & Molecular Biology
Takashi Goda, Hiroshi Teramura, Miki Suehiro, Kengo Kanamaru, Hideo Kawaguchi, Chiaki Ogino, Akihiko Kondo, Masanori Yamasaki
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2016)
Article
Pharmacology & Pharmacy
Tomohide Uno, Yuichiro Obe, Chika Ogura, Tatsushi Goto, Kohei Yamamoto, Masahiko Nakamura, Kengo Kanamaru, Hiroshi Yamagata, Hiromasa Imaishi
BIOPHARMACEUTICS & DRUG DISPOSITION
(2013)
Article
Pharmacology & Pharmacy
Tomohide Uno, Ryosuke Nakano, Risa Kitagawa, Mai Okada, Kengo Kanamaru, Shinji Takenaka, Yuichi Uno, Hiromasa Imaishi
BIOPHARMACEUTICS & DRUG DISPOSITION
(2018)
Article
Cell Biology
Tomohide Uno, Kazuki Sakamoto, Yuri Isoyama, Susumu Hiragaki, Yuichi Uno, Kengo Kanamaru, Hiroshi Yamagata, Michihiro Takagi, Akira Mizoguchi, Makio Takeda
HISTOCHEMISTRY AND CELL BIOLOGY
(2013)
Article
Cell Biology
Tomohide Uno, Yuri Isoyama, Kazuki Sakamoto, Yuichi Uno, Katsuhiko Sakamoto, Kengo Kanamaru, Hiroshi Yamagata, Michihiro Takagi, Akira Mizoguchi, Makio Takeda
HISTOCHEMISTRY AND CELL BIOLOGY
(2014)
Article
Cell Biology
Tomohide Uno, Yusuke Ozakiya, Masayuki Furutani, Katsuhiko Sakamoto, Yuichi Uno, Hideyuki Kajiwara, Kengo Kanamaru, Akira Mizoguchi
HISTOCHEMISTRY AND CELL BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Yu Takenouchi, Haruka Nakajima, Kengo Kanamaru, Shigeo Takumi
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2011)
Article
Plant Sciences
Hamad Abu Zahra, Satoru Kuwamoto, Tomohide Uno, Kengo Kanamaru, Hiroshi Yamagata
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2014)
Article
Biotechnology & Applied Microbiology
Kiyotaka Y. Hara, Masaru Saito, Hiroko Kato, Kana Morikawa, Hiroshi Kikukawa, Hironari Nomura, Takanori Fujimoto, Yoko Hirono-Hara, Shigeyuki Watanabe, Kengo Kanamaru, Akihiko Kondo
MICROBIAL CELL FACTORIES
(2019)
Article
Plant Sciences
Alvin Sanjaya, Yusuke Kazama, Kotaro Ishii, Ryohsuke Muramatsu, Kengo Kanamaru, Sumie Ohbu, Tomoko Abe, Makoto T. Fujiwara
Summary: Argon-ion beam mutagenesis induced a pale green mutant of Arabidopsis thaliana, named Ar50-33-pg1, with significant shrinkage of mesophyll chloroplasts during the chlorotic process. Genetic analysis revealed a large deletion in chromosome V, including the EGY1 gene, which was found to be responsible for the pale phenotype. Ultrastructural analysis showed disassembled chloroplasts in Ar50-33-pg1 and egy1, indicating the crucial role of EGY1 in chloroplast maintenance.
Article
Pharmacology & Pharmacy
Tomohide Uno, Ryosuke Nakano, Kengo Kanamaru, Shinji Takenaka, Yuichi Uno, Hiromasa Imaishi
BIOPHARMACEUTICS & DRUG DISPOSITION
(2017)
Article
Biochemistry & Molecular Biology
Tomohide Uno, Masayuki Furutani, Katsuhiko Sakamoto, Yuichi Uno, Kengo Kanamaru, Akira Mizoguchi, Susumu Hiragaki, Makio Takeda
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2017)