Fluorescence- and bioluminescence-based approaches to study GPCR ligand binding

Negative cooperativity across β1-adrenoceptor homodimers provides insights into the nature of the secondary low-affinity CGP 12177 β1-adrenoceptor binding conformation

(2015) Karolina Gherbi et al.   FASEB JOURNAL

Application of BRET to monitor ligand binding to GPCRs

(2015) Leigh A Stoddart et al.   NATURE METHODS

Detection of the secondary, low-affinity β1-adrenoceptor site in living cells using the fluorescent CGP 12177 derivative BODIPY-TMR-CGP

(2014) K Gherbi et al.   BRITISH JOURNAL OF PHARMACOLOGY

Characterization by flow cytometry of fluorescent, selective agonist probes of the A3 adenosine receptor

(2013) Eszter Kozma et al.   BIOCHEMICAL PHARMACOLOGY

Structural and biophysical characterisation of G protein-coupled receptor ligand binding using resonance energy transfer and fluorescent labelling techniques

(2013) Richard J. Ward et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors

(2013) Rajashri Sridharan et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Molecular determinants of orexin receptor-arrestin-ubiquitin complex formation

(2013) Werner C Jaeger et al.   BRITISH JOURNAL OF PHARMACOLOGY

The Concise Guide to PHARMACOLOGY 2013/14: G Protein-Coupled Receptors

(2013) Stephen P.H. Alexander et al.   BRITISH JOURNAL OF PHARMACOLOGY

The evolving small-molecule fluorescent-conjugate toolbox for Class A GPCRs

(2013) Andrea J Vernall et al.   BRITISH JOURNAL OF PHARMACOLOGY

SNAP-Tag Technology: A General Introduction

(2013) Katharina Kolberg et al.   CURRENT PHARMACEUTICAL DESIGN

Adenosine-A3 receptors in neutrophil microdomains promote the formation of bacteria-tethering cytonemes

(2013) Ross Corriden et al.   EMBO REPORTS

Time-resolved fluorescence ligand binding for G protein–coupled receptors

(2013) Alexander Emami-Nemini et al.   Nature Protocols

The IUPHAR/BPS Guide to PHARMACOLOGY: an expert-driven knowledgebase of drug targets and their ligands

(2013) Adam J. Pawson et al.   NUCLEIC ACIDS RESEARCH

Conversion of a non-selective adenosine receptor antagonist into A3-selective high affinity fluorescent probes using peptide-based linkers

(2013) Andrea J. Vernall et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

Engineered Luciferase Reporter from a Deep Sea Shrimp Utilizing a Novel Imidazopyrazinone Substrate

(2012) Mary P. Hall et al.   ACS Chemical Biology

Effect of enhanced Renilla luciferase and fluorescent protein variants on the Förster distance of Bioluminescence resonance energy transfer (BRET)

(2012) Helen Dacres et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Novel fluorescent antagonist as a molecular probe in A3 adenosine receptor binding assays using flow cytometry

(2012) Eszter Kozma et al.   BIOCHEMICAL PHARMACOLOGY

Fragment Screening at Adenosine-A3 Receptors in Living Cells Using a Fluorescence-Based Binding Assay

(2012) Leigh A. Stoddart et al.   CHEMISTRY & BIOLOGY

Molecular determinants of A2AR-D2R allosterism: role of the intracellular loop 3 of the D2R

(2012) Víctor Fernández-Dueñas et al.   JOURNAL OF NEUROCHEMISTRY

Fluorescence Polarization Screening for Allosteric Small Molecule Ligands of the Cholecystokinin Receptor

(2011) Kaleeckal G. Harikumar et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

Allosteric interactions across native adenosine-A3 receptor homodimers: quantification using single-cell ligand-binding kinetics

(2011) Lauren T. May et al.   FASEB JOURNAL

Temporal Profiling of Orexin Receptor-Arrestin-Ubiquitin Complexes Reveals Differences between Receptor Subtypes

(2011) Matthew B. Dalrymple et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Synthesis and Characterization of High-Affinity 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene-Labeled Fluorescent Ligands for Human β-Adrenoceptors

(2011) Jillian G. Baker et al.   JOURNAL OF MEDICINAL CHEMISTRY

Fluorescence anisotropy assay for pharmacological characterization of ligand binding dynamics to melanocortin 4 receptors

(2010) Santa Veiksina et al.   ANALYTICAL BIOCHEMISTRY

Homogeneous time-resolved fluorescence-based assay to screen for ligands targeting the growth hormone secretagogue receptor type 1a

(2010) Jean-Philippe Leyris et al.   ANALYTICAL BIOCHEMISTRY

Novel Alexa Fluor-488 labeled antagonist of the A2A adenosine receptor: Application to a fluorescence polarization-based receptor binding assay

(2010) Miklós Kecskés et al.   BIOCHEMICAL PHARMACOLOGY

Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands

(2010) Jillian G Baker et al.   BRITISH JOURNAL OF PHARMACOLOGY

Ligand binding assays at equilibrium: validation and interpretation

(2010) Edward C. Hulme et al.   BRITISH JOURNAL OF PHARMACOLOGY

The Effect of Allosteric Modulators on the Kinetics of Agonist-G Protein-Coupled Receptor Interactions in Single Living Cells

(2010) L. T. May et al.   MOLECULAR PHARMACOLOGY

A Homogeneous G Protein-Coupled Receptor Ligand Binding Assay Based on Time-Resolved Fluorescence Resonance Energy Transfer

(2008) Liaoyuan A. Hu et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES