Article
Biochemistry & Molecular Biology
Jie Xu, Huan Fang, Ya Su, Yue Kang, Deli Xu, Yuen Yee Cheng, Yi Nie, Hong Wang, Tianqing Liu, Kedong Song
Summary: Finding a bioink suitable for 3D bioprinting that can mimic the microenvironment of native skin and prevent bacterial infection remains a major challenge in skin tissue engineering. In this study, a tissue-specific extracellular matrix-based bioink was prepared, and a novel scaffold was obtained using an extrusion 3D bioprinting technology and dynamic hydrogen bonding method. The hybrid scaffold exhibited excellent properties including antibacterial activity, hemostatic effect, and biocompatibility.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Polymer Science
Mina Ghafouri Azar, Lucie Wiesnerova, Jana Dvorakova, Petra Chocholata, Omid Moztarzadeh, Jiri Dejmek, Vaclav Babuska
Summary: This research introduces a novel approach of incorporating different types of gelatins into polycaprolactone and poly (lactic-co-glycolic acid) films. The evaluation of these films includes morphology, mechanical properties, thermal stability, biodegradability, hemocompatibility, cell adhesion, proliferation, and cytotoxicity. The results show that the addition of gelatins alters the mechanical properties, biodegradability, and cell behavior of the films. These findings provide valuable insights for future tissue engineering applications involving gelatin incorporation.
Article
Biochemistry & Molecular Biology
Igor Rzhepakovsky, Sergey Piskov, Svetlana Avanesyan, Marina Sizonenko, Lyudmila Timchenko, Oxana Anfinogenova, Andrey Nagdalian, Andrey Blinov, Evgeniya Denisova, Stanislav Kochergin, Sergey Kubanov, Magomed Shakhbanov, Mohammad Ali Shariati, Mohammad S. Mubarak
Summary: Pure and porous bacterial cellulose scaffolds were synthesized using Medusomyces gisevii Sa-28 bacterial strain. Modification with gelatin increased the biocompatibility and solubility of the scaffolds. The scaffolds exhibited uniform distribution of pores, promoting new blood vessel growth and collagen fiber formation. The results suggest that these scaffolds can be used for engineering hard and soft tissues.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Resmi Rajalekshmi, Anusree Kaladevi Shaji, Roy Joseph, Anugya Bhatt
Summary: The development of a tissue-engineered construct for hepatic regeneration is challenging due to the lack of an optimum environment for hepatocyte growth. Injectable alginate dialdehyde-gelatin hydrogel incorporating fibrin was explored as a matrix for liver tissue engineering, showing promising support for the growth and function of HepG2 cells.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Shanzhu Guo, Yue Jiang, Jingjing Jiao, Yan Shi, Tao Zhu, Long Li
Summary: In this study, a gelatin-based biomimetic three-layer vascular scaffold with spatial alignment features mimicking the natural structure of blood vessels was successfully constructed using a sequential electrospinning strategy combined with folding and rolling manipulation. The scaffold could fully mimic the natural multi-layer structures of blood vessels and showed great potential for guiding the spatial arrangement of cells in blood vessels.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yunsheng Dong, Hui Xiao, Jie Wang, Tingting Yang, Naiqi Kang, Jiaxing Huang, Wei Cui, Yufei Liu, Qiang Yang, Shufang Wang
Summary: Constructing scaffolds with osteogenic activity through tissue engineering is an effective strategy for bone regeneration. This study used gelatin, silk fibroin, and Si3N4 to prepare silicon-functionalized bio-macromolecules composite scaffolds using 3DP technology. The scaffold showed good cyto-compatibility and promoted osteogenic differentiation of MSCs in vitro, and facilitated bone regeneration in vivo. The composite scaffold system has great potential for application in bone tissue engineering.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Sakchi Bhushan, Sandhya Singh, Tushar Kanti Maiti, Ankita Das, Ananya Barui, Leena R. Chaudhari, Meghnad G. Joshi, Dharm Dutt
Summary: Cell-free and cell-loaded constructs are used to repair critical-sized bone defects, with interest in enhancing three-dimensional scaffolds with free radical scavenging materials. In this study, CG-CNPs nanocomposite scaffolds with improved physico-chemical, mechanical, and biological properties were fabricated using freeze-drying. These scaffolds showed cyto-friendly behavior, antimicrobial activity, and demonstrated the best characteristics in terms of porosity, weight loss, compressive modulus, and Ca/P ratio. Therefore, the CG-CNP2 scaffolds are highly biocompatible and suitable for bone defect repair.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Amir Shamloo, Zahra Aghababaie, Homa Afjoul, Mehrzad Jami, Mina Razaghzadeh Bidgoli, Manouchehr Vossoughi, Ahmad Ramazani, Kambiz Kamyabhesari
Summary: Physically cross-linked hydrogels were developed by freezing-thawing method with different concentrations of honey for accelerated wound healing. The incorporation of honey increased the flexibility, antibacterial properties, and cell viability of hydrogels, while also accelerating wound healing.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Materials Science, Multidisciplinary
Pedram Iranmanesh, Mohammad Gowdini, Abbasali Khademi, Mohammad Dehghani, Mehran Latifi, Naif Alsaadi, Mehran Hemati, Ramin Mohammadi, Saeed Saber-Samandari, Davood Toghraie, Afrasyab Khan
Summary: The combination of 3D bioprinting technology with novel hydrogel and bioink has led to the successful fabrication of scaffolds with unique mechanical and biological responses. The addition of naproxen coating enhances the tensile strength of the scaffolds, showing promising compatibility with the human body.
JOURNAL OF MATERIALS RESEARCH AND TECHNOLOGY-JMR&T
(2021)
Article
Biochemistry & Molecular Biology
Hsia-Wei Liu, Wen-Ta Su, Ching-Yi Liu, Ching-Cheng Huang
Summary: A gelatin-based hydrogel scaffold with highly uniform pore size and biocompatibility was fabricated for cartilage tissue engineering using microfluidic 3D-foaming technology. The results showed that the scaffold has great potential in promoting chondrocyte proliferation, maintaining the structural stability of cartilage, and promoting cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Xiang Zeng, Qing-shuai Wei, Ji-chao Ye, Jun-hua Rao, Mei-guang Zheng, Yuan-huan Ma, Li-zhi Peng, Ying Ding, Bi-qin Lai, Ge Li, Shi-xiang Cheng, Eng-Ang Ling, Inbo Han, Yuan-shan Zeng
Summary: This study assessed the biosafety and efficacy of a three-dimensional gelatin sponge (3D-GS) scaffold in a non-human primate spinal cord injury model. The results demonstrated that the scaffold had good biocompatibility and significant effectiveness in the structural repair of injured spinal cord tissue, making it suitable for the treatment of patients with spinal cord injury.
Article
Biotechnology & Applied Microbiology
Phasuwit P. Phatchayawat, Arnon Khamkeaw, Supansa Yodmuang, Muenduen Phisalaphong
Summary: Biopolymers of bacterial cellulose, chitosan, alginate and gelatin were combined to fabricate 3D hydrogel scaffolds for tissue engineering. The composite scaffolds exhibited good architecture and biocompatibility, making them suitable for cartilage tissue engineering applications.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Polymer Science
Amaliya Rasyida, Salma Halimah, Ika Dewi Wijayanti, Sigit Tri Wicaksono, Haniffudin Nurdiansah, Yohannes Marudut Tua Silaen, Yatim Lailun Ni'mah, Hosta Ardhyananta, Agung Purniawan
Summary: This study investigated the interrelation between the addition of reduced graphene oxide (r-GO) into PVA/Alginate and their degradation and biocompatibility properties. The morphology of the composites was characterized using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), and the mechanical properties were determined through a compressive test. In vitro cytotoxicity and cell viability were assessed using an MTT assay. The presence of r-GO was found to have no significant effect on the morphology of the hydrogel, and the addition of 0.4% r-GO increased cell viability, indicating low toxicity. The studied composites showed minimal degradation and outstanding biocompatibility, making them attractive materials for tissue engineering applications.
Article
Biochemistry & Molecular Biology
Babitha Sumathy, Shiny Velayudhan
Summary: This study introduces a novel method for incorporating hair follicle derived stem cells in micro-pits of a bilayer skin equivalent using microneedles and a gelatin based scaffold. The microneedles are able to penetrate the upper layer of the scaffold and reach the lower layer, mimicking the natural niche of hair follicle stem cells. The bilayer system consists of electrospun layers that represent the epidermis and dermis, resulting in the formation of a complete skin model.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Multidisciplinary
Sneh Gautam, Shiv Dutt Purohit, Hemant Singh, Amit Kumar Dinda, Pravin D. Potdar, Chhavi Sharma, Chia -Fu Chou, Narayan Chandra Mishra
Summary: Bone tissue engineering has received attention for the treatment of bone injury. A tri-polymer scaffold (PCL-gelatin-chitosan) was fabricated through electrospinning and treated with nanohydroxyapatite. The modified scaffold showed a uniform distribution of nanohydroxyapatite particles on the surface of fibers.
MATERIALS TODAY COMMUNICATIONS
(2023)
Review
Polymer Science
Nur Izzah Md Fadilah, Isma Liza Mohd Isa, Wan Safwani Wan Kamarul Zaman, Yasuhiko Tabata, Mh Busra Fauzi
Summary: The use of nanoparticles in natural-based biomaterials has shown great potential in biomedical sciences, particularly in wound healing, tissue engineering, and regenerative medicine. This review summarizes the effects of nanoparticles on cellular interactions in wound healing and discusses the signaling pathways involved.
Review
Engineering, Biomedical
Isma Liza Mohd Isa, Sabarul Afian Mokhtar, Sunny A. Abbah, Mh Busra Fauzi, Aiden Devitt, Abhay Pandit
Summary: This review examines the understanding of intervertebral disc pathology, the concept of precision medicine, and the rationale of personalized biomaterial-based tissue engineering to enhance disc regeneration and alleviate discogenic pain.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Polymer Science
Nurkhuzaiah Kamaruzaman, Mh Busra Fauzi, Salma Mohamad Yusop
Summary: This study reported the extraction of water-soluble elastin from broiler skin and analyzed its chemical characteristics and toxicity. The extracted elastin showed high quality and can be used as an alternative source of biomaterial for tissue engineering applications.
Article
Chemistry, Physical
Izzat Zulkiflee, Ibrahim N. N. Amirrah, Nur Izzah Md Fadilah, M. F. Mohd Razip Wee, Salma Mohamad Yusop, Manira Maarof, Mh Busra Fauzi
Summary: Untreated skin wounds can delay healing and potentially lead to death from severe infection. Improper healing in these wounds often causes prolonged and excessive skin contraction, which can result in disfigurement and loss of tissue function. This study developed a bilayer biomatrix consisting of a gelatin/polyvinyl alcohol film and a collagen sponge fortified with elastin, which showed promising physicochemical and mechanical properties. The biomatrix could potentially be used as an alternative treatment for diabetic skin wound management.
Article
Biochemistry & Molecular Biology
Benson Koh, Nadiah Sulaiman, Mh Busra Fauzi, Jia Xian Law, Min Hwei Ng, Too Lih Yuan, Abdul Ghani Nur Azurah, Mohd Heikal Mohd Yunus, Ruszymah Bt Hj Idrus, Muhammad Dain Yazid
Summary: This study aimed to explore the potential of xeno-free three-dimensional cultures for mesenchymal stem cell (MSCs) expansion in clinical applications. Human serum and human platelet lysate were found to be suitable alternatives to replace foetal bovine serum in MSCs microcarrier cultures. Low glucose DMEM (LG) + human platelet (HPL) lysate was identified as the best xeno-free culture media, promoting high cell yield and maintaining MSC characteristics. This culture system showed improved immunomodulatory activities, suggesting its potential for future clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Ibrahim N. N. Amirrah, Izzat Zulkiflee, M. F. Mohd Razip Wee, Asad Masood, Kim S. S. Siow, Antonella Motta, Mh Busra Fauzi
Summary: This study aimed to develop an acellular, ready-to-use collagen bioscaffold with an antibacterial coating for the immediate treatment of skin wounds and to prevent infection. The crosslinker genipin demonstrated better physicochemical and mechanical properties, as well as enhanced biodegradability and biocompatibility compared to the non-crosslinked control. Similarly, the plasma-polymerised carvone coating on the crosslinked collagen scaffold showed promising results in terms of antibacterial properties and support for human skin cell proliferation and viability.
Article
Polymer Science
Nur Izzah Md Fadilah, Nazeha Ahmat, Looi Qi Hao, Manira Maarof, Nor Fadilah Rajab, Ruszymah Binti Hj Idrus, Mh Busra Fauzi
Summary: This study aimed to validate the biological safety of ovine tendon collagen type-I (OTC-I) under ISO and GLP settings. OTC-I was successfully extracted using acetic acid, and tests showed no adverse effects or abnormalities. Therefore, OTC-I is a potential medical device candidate for wound care management.
Review
Polymer Science
Nur Amalia Ra'oh, Rohaina Che Man, Mh Busra Fauzi, Norzana Abd Ghafar, Muhamad Ramdzan Buyong, Ng Min Hwei, Wan Haslina Wan Abdul Halim
Summary: In recent decades, researchers have made numerous modifications and advancements in designing the ideal corneal biomatrix for corneal epithelial cell (CECs) or limbal epithelial stem cell (LESC) carriers. However, the optimal strategies for corneal biomatrix design and its effects on cultured CECs or LESCs have yet to be discovered. This review summarizes recent optimization strategies for developing an ideal collagen biomatrix and its interactions with CECs and LESCs.
Review
Chemistry, Multidisciplinary
Fairuz Izan Muhammad Firdaus, Ab. Karim Nashihah, Mh. Busra Mohd Fauzi, Maarof Manira, Saim Aminuddin, Yogeswaran Lokanathan
Summary: In recent years, exploring respiratory therapy guided by cell-secreted substances in vitro has become popular. This review evaluated the efficacy of using conditioned medium (CM) collected in an in vitro respiratory epithelial model. The results showed that differentiated cells from respiratory and non-respiratory systems, such as mesenchymal stem cells (MSC), could improve proliferation, migration, attachment, and protect the respiratory epithelium against cytotoxicity. CM might be a potential regenerative therapy in respiratory illnesses, but further research is needed to justify its use in clinical practice.
APPLIED SCIENCES-BASEL
(2023)
Review
Pharmacology & Pharmacy
Rabiatul Adawiyah Razali, Ubashini Vijakumaran, Mh Busra Fauzi, Yogeswaran Lokanathan
Summary: This article systematically reviewed the available evidence to assess the efficacy of functional biomaterials after endoscopic sinus surgery (ESS). The analysis showed that chitosan, gelatin, hyaluronic acid, and starch-derived materials exhibit better endoscopic scores and significant potential for use in nasal packing.
Review
Biochemistry & Molecular Biology
Nur Izzah Md Fadilah, Shou Jin Phang, Nurkhuzaiah Kamaruzaman, Atiqah Salleh, Mazlan Zawani, Arka Sanyal, Manira Maarof, Mh Busra Fauzi
Summary: Natural antioxidant-incorporated biomaterials have shown promise in promoting skin wound healing and tissue regeneration. However, the low bioavailability of these biomaterials limits their therapeutic activity at the injury site. This review summarizes recent literature on the role of antioxidant-incorporated biomaterials and their mechanisms in promoting wound healing, supported by in vitro, in vivo, and clinical studies.
Review
Chemistry, Medicinal
Loai A. Elfawy, Chiew Yong Ng, Ibrahim N. Amirrah, Zawani Mazlan, Adzim Poh Yuen Wen, Nur Izzah Md Fadilah, Manira Maarof, Yogeswaran Lokanathan, Mh Busra Fauzi
Summary: Burns are a global public health issue that affects many people worldwide. Traditional burn wound treatment methods using synthetic materials may have negative environmental impacts and may not be biocompatible with the human body. Tissue engineering offers a promising alternative, with sustainable biomaterials such as collagen, cellulose, and chitosan showing potential in promoting wound healing and reducing infection risk. This review focuses on the use of multifunctional green biomaterials that can revolutionize burn treatment by promoting faster and more efficient healing while minimizing scarring and tissue damage.
Review
Biochemistry & Molecular Biology
Ab Karim Nashihah, Fairuz Izan Muhammad Firdaus, Mh. Busra Fauzi, Nadhratun Naiim Mobarak, Yogeswaran Lokanathan
Summary: Respiratory diseases have a significant impact on global health, particularly on the airway epithelium which is often targeted by inhaled allergens, irritants, or micro-organisms. In order to better understand these diseases, there is an urgent need for in vitro airway epithelial models that closely resemble the human respiratory system. Biomaterial scaffolds play a crucial role in tissue engineering as cell-support devices for transplantation. Recent studies have shown that these scaffolds have the potential to repair the airway epithelium and offer viable options for treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
