Review
Immunology
Omar-Javier Calixto, Maria-Alejandra Meneses-Toro, Edward-Camilo Vera-Parra, Juan -Manuel Bello-Gualtero, Consuelo Romero-Sanchez, Sandra J. Perdomo
Summary: This systematic review collected all published evidence regarding posttranslational modifications in Psoriatic arthritis (PsA) and evaluated the association between disease outcomes and specific posttranslational modifications. The study identified seven posttranslational modifications that were associated with diagnosis and prognosis.
AUTOIMMUNITY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Tsvetelina Batsalova, Balik Dzhambazov
Summary: Collagen type II (COL2) is significantly affected by autoimmune responses in rheumatoid arthritis (RA), and posttranslational modifications (PTMs) of COL2 have been implicated in RA autoimmunity. The discovery of anti-citrullinated protein response in RA has led to improved diagnostic assays and classification criteria for the disease. Immunological tolerance induced by modified COL2 peptides is considered as a potential strategy for RA therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Elisa X. Y. Lim, Julie A. A. Webster, Penny A. A. Rudd, Lara J. J. Herrero
Summary: This study used RNA sequencing to analyze the gene expression profiles of Ross River virus (RRV)-infected chondrocytes and bystander chondrocytes. The results showed similarities and differences in gene expression between infected and uninfected cells, indicating the importance of selecting therapeutic targets that minimize adverse effects on neighboring uninfected cells.
Article
Oncology
Yeongjoo Kim, Seungjae Shin, Sunyoung Kwon, Kisung Moon, Su -Vin Baek, Ahyoung Jo, Hyung-Sik Kim, Gue-Ho Hwang, Sangsu Bae, Yun Hak Kim, Sung-Yup Cho, Jung-Min Oh
Summary: N-6-methyladenosine (m6A) modification in RNA affects gene expression and RNA metabolism. This modification is regulated by various proteins, including writers, erasers, and readers. In this study, the authors investigated the effects of m6A modification on alternative splicing in cancer cells and found that METTL3 regulates m6A-dependent alternative splicing, particularly in cell cycle-related genes, through the modulation of splicing factors such as SFPQ.
AMERICAN JOURNAL OF CANCER RESEARCH
(2023)
Article
Materials Science, Biomaterials
Changwei Song, Shibo Xu, Linna Chang, Xingjun Zhao, Xifan Mei, Xiuli Ren, Zhenhua Chen
Summary: Combining EGCG with macrophage-derived extracellular vesicles showed promising results in treating rheumatoid arthritis, reducing arthritis-related protein expression and promoting cartilage repair. Animal experiments demonstrated that this treatment significantly reduced joint swelling and repaired cartilage.
REGENERATIVE BIOMATERIALS
(2021)
Article
Hematology
Patrick A. Murphy, Noor Jailkhani, Sarah-Anne Nicholas, Amanda M. Del Rosario, Jeremy L. Balsbaugh, Shahinoor Begum, Amy Kimble, Richard O. Hynes
Summary: Exposure to low and disturbed flow increases the risk of arterial endothelium erosion and rupture. Lack of specific FN splice isoforms in mice leads to impaired matrisome alterations, including reduced recruitment of FBLN1. The study highlights the significant impact of alternative splicing of FN on matrix composition in vascular inflammation and remodeling.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Chemistry, Physical
Joseph Atia Ayariga, Hanxiao Huang, Derrick Dean
Summary: Articular cartilage defects are common and contribute to poor quality of life and osteoarthritis. Tissue engineering, using decellularized scaffolds, shows promise for regenerating damaged cartilage. In this study, avian articular cartilage scaffolds reseeded with human chondrocytes were found to support cell survival, proliferation, and interaction. The decellularized scaffolds had a porous architecture and mechanical properties similar to native cartilage. These findings suggest that decellularized scaffolds are suitable for cartilage regeneration.
Review
Cell Biology
Ning Wang, Yue Hu, Zefeng Wang
Summary: Eukaryotic gene expression is tightly regulated at multiple levels, including alternative splicing, which generates multiple mRNA isoforms from a single gene. Epigenetic modifications play crucial roles in the regulation of alternative splicing by affecting transcription, splicing factor recruitment, and splicing factor expression/activity. Dysregulation of epigenetics and splicing is common in cancer and can have functional consequences. Understanding the complex regulation of gene expression layers can provide new insights into modulating disease-related splicing dysregulation.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Multidisciplinary Sciences
Zhong Li, Anil Mathew Tharappel, Jimin Xu, Yuekun Lang, Cathleen M. Green, Jing Zhang, Qishan Lin, Sudha Chaturvedi, Jia Zhou, Marlene Belfort, Hongmin Li
Summary: A small-molecule library was screened to find potent inhibitors of the Cne Prp8 intein, leading to the identification of compound 6G-318S with potential antifungal properties. These inhibitors showed specific action against Cryptococcus species and demonstrated synergistic effects with certain antimycotics. Their binding to the target Prp8 intein was covalent and mutation of the active-site residue reduced binding, highlighting their potential as a new class of antifungal drugs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Zeinab Rekad, Valerio Izzi, Rijuta Lamba, Delphine Ciais, Ellen Van Obberghen-Schilling
Summary: The extracellular matrix (ECM) is a fundamental component of tissue in multicellular organisms that regulates cellular processes and impacts cell fate decisions. The composition of the ECM varies across organs, developmental stages, and diseases, and most ECM genes undergo alternative splicing events to enhance complexity. Recent studies have linked alternative splicing of ECM isoforms to cancer, suggesting its role in tumor progression and stromal activation.
Review
Oncology
Francisco Gimeno-Valiente, Gerardo Lopez-Rodas, Josefa Castillo, Luis Franco
Summary: This review focuses on the interconnections between epigenetics and alternative splicing in the development of cancer. It discusses the mechanisms involved in these interconnections and the potential diagnostic and therapeutic tools that can be derived from them. The reversible nature of epigenetic alterations and the possibility of correcting aberrant alternative splicing offer promising therapeutic possibilities for cancer treatment.
Review
Immunology
Pingping Ren, Luying Lu, Shasha Cai, Jianghua Chen, Weiqiang Lin, Fei Han
Summary: Alternative splicing is a complex regulatory mechanism that affects a large percentage of genes and organs, aberrant splicing can lead to various diseases, though its association with autoimmune diseases requires further examination.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Plant Sciences
Wei Lan, Yuhao Qiu, Yun Xu, Yalin Liu, Ying Miao
Summary: Alternative splicing plays a critical role in plant development and response to environmental stress. Post-translational modification, such as protein ubiquitination, has been found to regulate the activity and stability of spliceosomal proteins, providing another layer of regulation for alternative splicing. This review summarizes recent studies on ubiquitin and ubiquitin-like modification of spliceosome components and discusses the relationship between spliceosome and the ubiquitin/26S proteasome pathway in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Zerina Balic, Saurav Misra, Belinda Willard, Dieter P. Reinhardt, Suneel S. Apte, Dirk Hubmacher
Summary: ADAMTS proteases play important roles in the biosynthesis and breakdown of ECM molecules, with alternative splicing playing a significant role in regulating their proteolytic activity and cellular localization. This study characterizes the impact of alternative splicing on ADAMTS17, revealing two novel splice variants that affect protease activity through structural changes.
Review
Physiology
Dan Liu, Xingxing Li, Lin Zhang, Bin Hu, Sang Hu, Xiao Zhang, Jing Hu
Summary: Osteoarthritis is a common degenerative joint disease that mainly damages articular cartilage, causing pain, swelling, and stiffness. The treatment options are limited and the common drugs have frequent and serious adverse reactions. The development of safer and more effective anti-osteoarthritis drugs is essential and urgent. This review summarizes recent advances in pharmacological treatment, focusing on inhibitors targeting cartilage remodeling and optimizing the dosage form of traditional drugs to reduce adverse effects.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Upendar G. Reddy, Joerg Axthelm, Patrick Hoffmann, Nandraj Taye, Steve Glaeser, Helmar Goerls, Samantha L. Hopkins, Winfried Plass, Ute Neugebauer, Sylvestre Bonnet, Alexander Schiller
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Biochemistry & Molecular Biology
Dirk Hubmacher, Nandaraj Taye, Zerina Balic, Stetson Thacker, Sheila M. Adams, David E. Birk, Ronen Schweitzer, Suneel S. Apte
Article
Orthopedics
Nandaraj Taye, Stylianos Z. Karoulias, Dirk Hubmacher
JOURNAL OF ORTHOPAEDIC RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Stylianos Z. Karoulias, Aude Beyens, Zerina Balic, Sofie Symoens, Anthony Vandersteen, Andrea L. Rideout, John Dickinson, Bert Callewaert, Dirk Hubmacher
Article
Oncology
Aftab Alam, Nandaraj Taye, Sonal Patel, Milind Thube, Jayati Mullick, Vibhuti Kumar Shah, Richa Pant, Tanaya Roychowdhurey, Nilanjan Banerjee, Subhranosu Chatterjee, Rittwika Bhattachatya, Rini Roy, Ashis Mukhopadhyay, Devraj Mogare, Samit Chattopadhyay
Article
Multidisciplinary Sciences
Nandaraj Taye, Sarah Stanley, Dirk Hubmacher
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2020)
Review
Biochemistry & Molecular Biology
Stylianos Z. Karoulias, Nandaraj Taye, Sarah Stanley, Dirk Hubmacher
Article
Biochemistry & Molecular Biology
Apoorva Parulekar, Arpankumar Choksi, Nandaraj Taye, Kumar V. S. Totakura, Priyanka Firmal, Gopal C. Kundu, Samit Chattopadhyay
Summary: The study reveals that down-regulation of SMAR1 leads to the activation of hTERT, resulting in an increase in the cancer stem cell phenotype in colorectal cancer cells. SMAR1 acts as a transcriptional repressor of hTERT, and its depletion promotes the total CD133(+)CD44(+) population and enhanced sphere-forming ability of colorectal cancer cells.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nandaraj Taye, Mukti Singh, Clair Baldock, Dirk Hubmacher
Summary: Myogenesis is regulated by MYOD1 and involves ADAMTSL2, which acts as a signal hub to integrate WNT, TGF6, and potentially other signaling pathways in the dynamic microenvironment of differentiating myoblasts during skeletal muscle development and regeneration. ADAMTSL2 depletion leads to impaired myoblast differentiation and abnormal skeletal muscle architecture. Mechanistically, ADAMTSL2 enhances WNT signaling by binding to WNT ligands and receptors, and a WNT-binding ADAMTSL2 peptide is sufficient to promote myogenesis in vitro.
Article
Cell & Tissue Engineering
B. Satz-Jacobowitz, N. Taye, S. Z. Karoulias, D. Hubmacher
Summary: Biochemical and biophysical factors are important to consider when modeling in vivo cellular behavior in vitro. One underappreciated factor is the high concentration of macromolecules present in vivo, which is typically not simulated in standard cell culture conditions. This study found that adding inert macromolecules to cell culture medium can better mimic the high concentration environments found in vivo, leading to enhanced deposition of fibrillin-1.
EUROPEAN CELLS & MATERIALS
(2022)
Article
Endocrinology & Metabolism
Stylianos-Zafeirios Karoulias, Maria Pitou, Rigini Papi, Paraskevas Lamprou, Theodora Choli-Papadopoulou
Summary: The study identified functional domains of growth factor BMP-2 that are crucial for osteogenesis, particularly the C-terminal region and the 86-AISMLYLDEN-95 sequence. Amino acids Ser88 and Leu90 were found to be important for receptor binding and osteogenic efficacy. These domains mainly signal through the Smad pathway and not the ERK1/2 pathway. The findings have significant implications for clinical applications in biomaterial generation and orthopedic fracture healing.
Article
Chemistry, Inorganic & Nuclear
Vadde Ramu, Sunil Aute, Nandaraj Taye, Rweetuparna Guha, Michael G. Walker, Devaraj Mogare, Apoorva Parulekar, Jim A. Thomas, Samit Chattopadhyay, Amitava Das
DALTON TRANSACTIONS
(2017)