Producing Vaccines against Enveloped Viruses in Plants: Making the Impossible, Difficult

Plant-Produced Antigen Displaying Virus-Like Particles Evokes Potent Antibody Responses against West Nile Virus in Mice

(2021) Junyun He et al.   Vaccines

Efficient Production of Chimeric Hepatitis B Virus-Like Particles Bearing an Epitope of Hepatitis E Virus Capsid by Transient Expression in Nicotiana benthamiana

(2021) Gergana Zahmanova et al.   Life-Basel

Plant molecular farming for the production of valuable proteins – Critical evaluation of achievements and future challenges

(2021) Stefan Schillberg et al.   JOURNAL OF PLANT PHYSIOLOGY

Phase 1 randomized trial of a plant-derived virus-like particle vaccine for COVID-19

(2021) Brian J. Ward et al.   NATURE MEDICINE

Molecular farming – The slope of enlightenment

(2020) Rainer Fischer et al.   BIOTECHNOLOGY ADVANCES

Plant-made HIV vaccines and potential candidates

(2020) Jocelyne Tremouillaux-Guiller et al.   CURRENT OPINION IN BIOTECHNOLOGY

Co‐expression of human calreticulin significantly improves the production of HIV gp140 and other viral glycoproteins in plants

(2020) E Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

Hepatitis B Virus (HBV) Subviral Particles as Protective Vaccines and Vaccine Platforms

(2020) Joan Kha-Tu Ho et al.   Viruses-Basel

Plant-Produced Recombinant Influenza A Virus Candidate Vaccine Based on Flagellin Linked to Conservative Fragments of M2 Protein and Hemagglutintin

(2020) Elena A. Blokhina et al.   Plants-Basel

The Immunogenicity of a Novel Chimeric Hemagglutinin-Neuraminidase-Fusion Antigen from Newcastle Disease Virus by Oral Delivery of Transgenic Canola Seeds to Chickens

(2020) Mohammad Javad Motamedi et al.   MOLECULAR BIOTECHNOLOGY

Oral delivery of maize-produced porcine epidemic diarrhea virus spike protein elicits neutralizing antibodies in pigs

(2020) Erin Egelkrout et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Engineering the Plant Secretory Pathway for the Production of Next-Generation Pharmaceuticals

(2020) Emmanuel A. Margolin et al.   TRENDS IN BIOTECHNOLOGY

Potential Applications of Plant Biotechnology against SARS-CoV-2

(2020) Teresa Capell et al.   TRENDS IN PLANT SCIENCE

Codelivery of improved immune complex and virus-like particle vaccines containing Zika virus envelope domain III synergistically enhances immunogenicity

(2020) Andrew G. Diamos et al.   VACCINE

Efficacy, immunogenicity, and safety of a plant-derived, quadrivalent, virus-like particle influenza vaccine in adults (18–64 years) and older adults (≥65 years): two multicentre, randomised phase 3 trials

(2020) Brian J Ward et al.   LANCET

Plant‐made dengue virus‐like particles produced by co‐expression of structural and non‐structural proteins induce a humoral immune response in mice

(2020) Daniel Ponndorf et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant-Derived Trimeric CO-26K-Equivalent Epitope Induced Neutralizing Antibodies Against Porcine Epidemic Diarrhea Virus

(2020) Thuong Thi Ho et al.   Frontiers in Immunology

Immunization with Plant-Derived Multimeric H5 Hemagglutinins Protect Chicken against Highly Pathogenic Avian Influenza Virus H5N1

(2020) Hoang Trong Phan et al.   Vaccines

Early Stage Development of a Newcastle Disease Vaccine Candidate in Corn

(2020) Naila Shahid et al.   Frontiers in Veterinary Science

Significantly Improved Recovery of Recombinant Sonchus Yellow Net Rhabdovirus by Expressing the Negative-Strand Genomic RNA

(2020) Xiaonan Ma et al.   Viruses-Basel

Emerging viruses in aquaculture

(2019) Frederick SB Kibenge   Current Opinion in Virology

Improved expression of porcine epidemic diarrhea antigen by fusion with cholera toxin B subunit and chloroplast transformation in Nicotiana tabacum

(2019) Nguyen-Quang-Duc Tien et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Design of a multiepitopic Zaire ebolavirus protein and its expression in plant cells

(2019) Ricardo Nieto-Gómez et al.   JOURNAL OF BIOTECHNOLOGY

Epitope Presentation of Dengue Viral Envelope Glycoprotein Domain III on Hepatitis B Core Protein Virus-Like Particles Produced in Nicotiana benthamiana

(2019) Ee Leen Pang et al.   Frontiers in Plant Science

Efficacy of a plant‐produced virus like particle vaccine in chickens challenged with Influenza A H6N2 virus

(2019) Tanja Smith et al.   PLANT BIOTECHNOLOGY JOURNAL

Immunogenicity and safety of a quadrivalent plant-derived virus like particle influenza vaccine candidate—Two randomized Phase II clinical trials in 18 to 49 and ≥50 years old adults

(2019) Stéphane Pillet et al.   PLoS One

Improving plant transient expression through the rational design of synthetic 5′ and 3′ untranslated regions

(2019) Hadrien Peyret et al.   Plant Methods

Production and Immunogenicity of Soluble Plant-Produced HIV-1 Subtype C Envelope gp140 Immunogens

(2019) Emmanuel Margolin et al.   Frontiers in Plant Science

Parenteral–Oral Immunization with Plant-Derived HBcAg as a Potential Therapeutic Vaccine against Chronic Hepatitis B

(2019) Marcin Pyrski et al.   Vaccines

ICTV Virus Taxonomy Profile: Rhabdoviridae

(2018) Peter J. Walker et al.   JOURNAL OF GENERAL VIROLOGY

Production of complex viral glycoproteins in plants as vaccine immunogens

(2018) Emmanuel Margolin et al.   PLANT BIOTECHNOLOGY JOURNAL

A plant-produced vaccine protects mice against lethal West Nile virus infection without enhancing Zika or dengue virus infectivity

(2018) Huafang Lai et al.   VACCINE

Humoral and cell-mediated immune responses to H5N1 plant-made virus-like particle vaccine are differentially impacted by alum and GLA-SE adjuvants in a Phase 2 clinical trial

(2018) Stéphane Pillet et al.   npj Vaccines

Chimaeric Rift Valley Fever Virus-Like Particle Vaccine Candidate Production in Nicotiana benthamiana

(2018) Sandiswa Mbewana et al.   Biotechnology Journal

Production of tetravalent dengue virus envelope protein domain III based antigens in lettuce chloroplasts and immunologic analysis for future oral vaccine development

(2018) André van Eerde et al.   PLANT BIOTECHNOLOGY JOURNAL

Lettuce-produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination

(2017) Jihong Liu Clarke et al.   PLANT BIOTECHNOLOGY JOURNAL

Plant-produced Zika virus envelope protein elicits neutralizing immune responses that correlate with protective immunity against Zika virus in mice

(2017) Ming Yang et al.   PLANT BIOTECHNOLOGY JOURNAL

Viral hemorrhagic septicemia virus glycoprotein production in tobacco

(2017) Nguyen-Quang-Duc Tien et al.   PROTEIN EXPRESSION AND PURIFICATION

Major findings and recent advances in virus–like particle (VLP)-based vaccines

(2017) Mona O. Mohsen et al.   SEMINARS IN IMMUNOLOGY

High accumulation in tobacco seeds of hemagglutinin antigen from avian (H5N1) influenza

(2017) Yanaysi Ceballo et al.   TRANSGENIC RESEARCH

Comparison of the protective efficacy between single and combination of recombinant adenoviruses expressing complete and truncated glycoprotein, and nucleoprotein of the pathogenic street rabies virus in mice

(2017) Ha-Hyun Kim et al.   Virology Journal

Plant-made polio type 3 stabilized VLPs—a candidate synthetic polio vaccine

(2017) Johanna Marsian et al.   Nature Communications

Structure and organization of paramyxovirus particles

(2017) Robert M Cox et al.   Current Opinion in Virology

Production of Japanese Encephalitis Virus Antigens in Plants Using Bamboo Mosaic Virus-Based Vector

(2017) Tsung-Hsien Chen et al.   Frontiers in Microbiology

Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein

(2017) Ngoc Bich Pham et al.   JOURNAL OF NANOBIOTECHNOLOGY

Virus-like particles that display Zika virus envelope protein domain III induce potent neutralizing immune responses in mice

(2017) Ming Yang et al.   Scientific Reports

A plant-derived quadrivalent virus like particle influenza vaccine induces cross-reactive antibody and T cell response in healthy adults

(2016) Stéphane Pillet et al.   CLINICAL IMMUNOLOGY

Molecular pharming — VLPs made in plants

(2016) Johanna Marsian et al.   CURRENT OPINION IN BIOTECHNOLOGY

Immunogenicity of an S1D epitope from porcine epidemic diarrhea virus and cholera toxin B subunit fusion protein transiently expressed in infiltrated Nicotiana benthamiana leaves

(2016) Nguyen-Xuan Huy et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Production of dengue virus envelope protein domain III-based antigens in tobacco chloroplasts using inducible and constitutive expression systems

(2016) Johanna Gottschamel et al.   PLANT MOLECULAR BIOLOGY

Arabidopsis thaliana plants expressing Rift Valley fever virus antigens: Mice exhibit systemic immune responses as the result of oral administration of the transgenic plants

(2016) Irina Kalbina et al.   PROTEIN EXPRESSION AND PURIFICATION

Antigen Production in Plant to Tackle Infectious Diseases Flare Up: The Case of SARS

(2016) Olivia C. Demurtas et al.   Frontiers in Plant Science

Immunological Characterization of Plant-Based HIV-1 Gag/Dgp41 Virus-Like Particles

(2016) Sarah A. Kessans et al.   PLoS One

Extraction and downstream processing of plant-derived recombinant proteins

(2015) J.F. Buyel et al.   BIOTECHNOLOGY ADVANCES

Plant-made vaccines against West Nile virus are potent, safe, and economically feasible

(2015) Qiang Chen   Biotechnology Journal

Rapid high-yield expression of a candidate influenza vaccine based on the ectodomain of M2 protein linked to flagellin in plants using viral vectors

(2015) Eugenia S. Mardanova et al.   BMC BIOTECHNOLOGY

Co-expression of hepatitis C virus polytope–HBsAg and p19-silencing suppressor protein in tobacco leaves

(2015) Sara Mohammadzadeh et al.   PHARMACEUTICAL BIOLOGY

When plant virology metAgrobacterium: the rise of the deconstructed clones

(2015) Hadrien Peyret et al.   PLANT BIOTECHNOLOGY JOURNAL

Expression of H3N2 nucleoprotein in maize seeds and immunogenicity in mice

(2015) Hartinio N. Nahampun et al.   PLANT CELL REPORTS

Chikungunya virus vaccines: Current strategies and prospects for developing plant-made vaccines

(2015) Jorge A. Salazar-González et al.   VACCINE

Plant-derived H7 VLP vaccine elicits protective immune response against H7N9 influenza virus in mice and ferrets

(2015) S. Pillet et al.   VACCINE

Novel vaccination approach for dengue infection based on recombinant immune complex universal platform

(2015) Mi-Young Kim et al.   VACCINE

Rescue of a Plant Negative-Strand RNA Virus from Cloned cDNA: Insights into Enveloped Plant Virus Movement and Morphogenesis

(2015) Qiang Wang et al.   PLoS Pathogens

Plant-made vaccines against West Nile virus are potent, safe, and economically feasible

(2015) Qiang Chen   Biotechnology Journal

High immunogenicity of plant-produced candidate influenza vaccine based on the M2e peptide fused to flagellin

(2015) Eugenia S. Mardanova et al.   Bioengineered

Tandem Fusion of Hepatitis B Core Antigen Allows Assembly of Virus-Like Particles in Bacteria and Plants with Enhanced Capacity to Accommodate Foreign Proteins

(2015) Hadrien Peyret et al.   PLoS One

Expression of Rabies Glycoprotein and Ricin Toxin B Chain (RGP–RTB) Fusion Protein in Tomato Hairy Roots: A Step Towards Oral Vaccination for Rabies

(2014) Ankit Singh et al.   MOLECULAR BIOTECHNOLOGY

Influence of Elastin-Like Polypeptide and Hydrophobin on Recombinant Hemagglutinin Accumulations in Transgenic Tobacco Plants

(2014) Hoang Trong Phan et al.   PLoS One

A new adjuvanted nanoparticle-based H1N1 influenza vaccine induced antigen-specific local mucosal and systemic immune responses after administration into the lung

(2014) Vanessa Neuhaus et al.   VACCINE

Plant-based vaccines against viruses

(2014) Edward P Rybicki   Virology Journal

A Plant-Produced Antigen Elicits Potent Immune Responses against West Nile Virus in Mice

(2014) Junyun He et al.   Biomed Research International

Is An Oral Plant-based Vaccine against Hepatitis B Virus Possible?

(2013) Tomasz Pniewski   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

The Use of Transient Expression Systems for the Rapid Production of Virus-like Particles in Plants

(2013) Eva Thuenemann et al.   CURRENT PHARMACEUTICAL DESIGN

The Twenty-Year Story of a Plant-Based Vaccine Against Hepatitis B: Stagnation or Promising Prospects?

(2013) Tomasz Pniewski   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

ELPylated haemagglutinins produced in tobacco plants induce potentially neutralizing antibodies against H5N1 viruses in mice

(2013) Hoang T. Phan et al.   PLANT BIOTECHNOLOGY JOURNAL

A method for rapid production of heteromultimeric protein complexes in plants: assembly of protective bluetongue virus-like particles

(2013) Eva C. Thuenemann et al.   PLANT BIOTECHNOLOGY JOURNAL

Biological and biochemical characterization of HIV-1 Gag/dgp41 virus-like particles expressed inNicotiana benthamiana

(2013) Sarah A. Kessans et al.   PLANT BIOTECHNOLOGY JOURNAL

How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture?

(2013) Jihong Liu Clarke et al.   PLANT MOLECULAR BIOLOGY

Rapid High-Level Production of Functional HIV Broadly Neutralizing Monoclonal Antibodies in Transient Plant Expression Systems

(2013) Yvonne Rosenberg et al.   PLoS One

Safety and immunogenicity of a plant-produced recombinant monomer hemagglutinin-based influenza vaccine derived from influenza A (H1N1)pdm09 virus: A Phase 1 dose-escalation study in healthy adults

(2013) James F. Cummings et al.   VACCINE

A plant-produced H1N1 trimeric hemagglutinin protects mice from a lethal influenza virus challenge

(2013) Yoko Shoji et al.   Human Vaccines & Immunotherapeutics

Single-dose monomeric HA subunit vaccine generates full protection from influenza challenge

(2013) Jyothi K Mallajosyula et al.   Human Vaccines & Immunotherapeutics

Plant-produced recombinant influenza vaccine based on virus-like HBc particles carrying an extracellular domain of M2 protein

(2012) N. V. Ravin et al.   BIOCHEMISTRY-MOSCOW

Setting up a platform for plant-based influenza virus vaccine production in South Africa

(2012) Elizabeth Mortimer et al.   BMC BIOTECHNOLOGY

Immunogenicity of a neutralizing epitope from porcine epidemic diarrhea virus: M cell targeting ligand fusion protein expressed in transgenic rice calli

(2012) Nguyen-Xuan Huy et al.   PLANT CELL REPORTS

Functional ectodomain of the hemagglutinin-neuraminidase protein is expressed in transgenic tobacco cells as a candidate vaccine against Newcastle disease virus

(2012) Kok Song Lai et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Induction of a protective immune response to rabies virus in sheep after oral immunization with transgenic maize, expressing the rabies virus glycoprotein

(2012) Elizabeth Loza-Rubio et al.   VACCINE

Safety and Immunogenicity of a Plant-Produced Recombinant Hemagglutinin-Based Influenza Vaccine (HAI-05) Derived from A/Indonesia/05/2005 (H5N1) Influenza Virus: A Phase 1 Randomized, Double-Blind, Placebo-Controlled, Dose-Escalation Study in Healthy Adults

(2012) Jessica Chichester et al.   Viruses-Basel

Mice Orally Immunized with a Transgenic Plant Expressing the Glycoprotein of Crimean-Congo Hemorrhagic Fever Virus

(2011) S. M. Ghiasi et al.   Clinical and Vaccine Immunology

Plant-based rapid production of recombinant subunit hemagglutinin vaccines targeting H1N1 and H5N1 influenza

(2011) Yoko Shoji et al.   Human vaccines & immunotherapeutics

Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana

(2011) Waranyoo Phoolcharoen et al.   PLANT BIOTECHNOLOGY JOURNAL

Expression of dengue-3 premembrane and envelope polyprotein in lettuce chloroplasts

(2011) Anderson Paul Kanagaraj et al.   PLANT MOLECULAR BIOLOGY

A nonreplicating subunit vaccine protects mice against lethal Ebola virus challenge

(2011) W. Phoolcharoen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structure of the Newcastle disease virus hemagglutinin-neuraminidase (HN) ectodomain reveals a four-helix bundle stalk

(2011) P. Yuan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Expression of dengue virus E glycoprotein domain III in non-nicotine transgenic tobacco plants

(2010) Mi-Young Kim et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Production of a heat-labile enterotoxin B subunit-porcine epidemic diarrhea virus-neutralizing epitope fusion protein in transgenic lettuce (Lactuca sativa)

(2010) Nguyen-Xuan Huy et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Exploring different strategies to express Dengue virus envelope protein in a plant system

(2010) Carolina Andrea Martínez et al.   BIOTECHNOLOGY LETTERS

Low-dose oral immunization with lyophilized tissue of herbicide-resistant lettuce expressing hepatitis B surface antigen for prototype plant-derived vaccine tablet formulation

(2010) Tomasz Pniewski et al.   JOURNAL OF APPLIED GENETICS

In vitro stability of Cucumber mosaic virus nanoparticles carrying a Hepatitis C virus-derived epitope under simulated gastrointestinal conditions and in vivo efficacy of an edible vaccine

(2010) M. Nuzzaci et al.   JOURNAL OF VIROLOGICAL METHODS

Expression of a Cholera Toxin B Subunit-Neutralizing Epitope of the Porcine Epidemic Diarrhea Virus Fusion Gene in Transgenic Lettuce (Lactuca sativa L.)

(2010) Nguyen-Xuan Huy et al.   MOLECULAR BIOTECHNOLOGY

A virus-like particle vaccine for epidemic Chikungunya virus protects nonhuman primates against infection

(2010) Wataru Akahata et al.   NATURE MEDICINE

Preclinical and Clinical Development of Plant-Made Virus-Like Particle Vaccine against Avian H5N1 Influenza

(2010) Nathalie Landry et al.   PLoS One

Generation and immunogenicity of Japanese encephalitis virus envelope protein expressed in transgenic rice

(2009) Yuanyuan Wang et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Expression of Hemagglutinin-Neuraminidase glycoprotein of Newcastle Disease Virus in agroinfiltrated Nicotiana benthamiana plants

(2009) Evangelina Gómez et al.   JOURNAL OF BIOTECHNOLOGY

Cowpea mosaic virus chimeric particles bearing the ectodomain of matrix protein 2 (M2E) of the influenza a virus: Production and characterization

(2009) Yu. A. Meshcheryakova et al.   MOLECULAR BIOLOGY

High-level expression of the HIV-1 Pr55gag polyprotein in transgenic tobacco chloroplasts

(2009) Nunzia Scotti et al.   PLANTA

Boosted expression of the SARS-CoV nucleocapsid protein in tobacco and its immunogenicity in mice

(2009) Nuoyan Zheng et al.   VACCINE

Expression of HIV-1 antigens in plants as potential subunit vaccines

(2008) Ann Meyers et al.   BMC BIOTECHNOLOGY

A plant-produced influenza subunit vaccine protects ferrets against virus challenge

(2008) Vadim Mett et al.   Influenza and Other Respiratory Viruses

High-yield rapid production of hepatitis B surface antigen in plant leaf by a viral expression system

(2008) Zhong Huang et al.   PLANT BIOTECHNOLOGY JOURNAL

Influenza virus-like particles produced by transient expression inNicotiana benthamianainduce a protective immune response against a lethal viral challenge in mice

(2008) Marc-André D’Aoust et al.   PLANT BIOTECHNOLOGY JOURNAL

Expression of the rabies virus nucleoprotein in plants at high-levels and evaluation of immune responses in mice

(2008) Irene Perea Arango et al.   PLANT CELL REPORTS

Improvement of PVX/CMV CP expression tool for display of short foreign antigens

(2008) Angela Natilla et al.   PROTEIN EXPRESSION AND PURIFICATION

Plant-expressed HA as a seasonal influenza vaccine candidate

(2008) Yoko Shoji et al.   VACCINE

Immunogenicity and tolerance following HIV-1/HBV plant-based oral vaccine administration

(2008) Denise Guetard et al.   VACCINE

Plant-derived hemagglutinin protects ferrets against challenge infection with the A/Indonesia/05/05 strain of avian influenza

(2008) Yoko Shoji et al.   VACCINE