Article
Microbiology
Ngwafu Nancy Ngwasiri, Norbert W. Brattig, Dieudonne Ndjonka, Eva Liebau, Archile Paguem, Dustin Leusder, Manchang Tanyi Kingsley, Albert Eisenbarth, Alfons Renz, Achukwi Mbunkah Daniel
Summary: The study identified an O. ochengi galectin gene, confirmed its immunogenicity, and found a high degree of identity between O. ochengi and O. volvulus galectin, with similar immune reactivity. These results suggest the suitability of the cattle O. ochengi model for O. volvulus infection research.
Article
Parasitology
James Rooney, Timothy L. Williams, Holly M. Northcote, Fiona E. Karet Frankl, Daniel R. G. Price, Alasdair J. Nisbet, Russell M. Morphew, Cinzia Cantacessi
Summary: This study investigates the ability of gastrointestinal helminth parasites to alter the composition of the host gut microbiome. They analyze the excretory-secretory products of Teladorsagia circumcincta and identify molecules with antimicrobial activity. The study suggests that these molecules may have bactericidal and/or bacteriostatic properties.
PARASITES & VECTORS
(2022)
Article
Immunology
Benjamin Hamid, Friederike Ebner, Lalita Bechtold, Arkadi Kundik, Sebastian Rausch, Susanne Hartmann
Summary: The study found that the relative frequencies of DC subsets in porcine blood differ, with A. suum E/S showing differential modulation on different DC subsets, suppressing pDC and cDC1 while upregulating cDC2.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Armin Mirzapour-Kouhdasht, Marzieh Moosavi-Nasab, Young-Min Kim, Jong-Bang Eun
Summary: The study aimed to produce gelatin peptides from fish by-products for potential application in carbonated beverages, showing high antioxidant and antibacterial activities. The fraction < 3 kDa exhibited the highest peptide concentration and activities, but these biological activities were significantly decreased after in vitro gastrointestinal digestion. The F < 3 kDa could be safely used for fortification of carbonated beverages due to its properties and cytotoxicity analysis.
Article
Parasitology
Lingqin Wu, Wenhui Yin, Jutai Wen, Shuying Wang, Huihui Li, Xiaoli Wang, Weixiao Zhang, Shuyao Duan, Qiuyu Zhu, Erhe Gao, Shili Wu, Bin Zhan, Rui Zhou, Xiaodi Yang
Summary: The study showed that treatment with Ts-AES significantly improved cardiac function and ventricular remodeling, reduced pathological damage and mortality in mice with MI. This was associated with decreased pro-inflammatory cytokine levels, increased regulatory cytokine expression, and promotion of macrophage polarization from M1 to M2 type in MI mice.
PARASITES & VECTORS
(2023)
Article
Parasitology
Jing Wang, Bin Tang, Xihuo You, Xuepeng Cai, Wanzhong Jia, Xiaolei Liu, Mingyuan Liu, Xuemin Jin, Jing Ding
Summary: This study investigated the effects of Trichinella spiralis' excretory/secretory products (ESP) on the functions of immune cells. The results revealed the evasion and regulation mechanisms employed by the parasite, providing insights into its strategies to manipulate host immunity.
PARASITES & VECTORS
(2023)
Article
Microbiology
Zhuo Lan, Xiao-Lei Liu, Qing-Bo Lv, Min-Hao Zeng, Jun-Feng Gao, Qiao-Cheng Chang, Yuan-Yuan Chen, Chun-Ren Wang
Summary: This study identified differences in proteins present during different periods of Fasciola hepatica infection, providing valuable data for the diagnosis of fasciolosis and understanding of host-pathogen interactions.
Article
Immunology
Jiacheng Wu, Yuqi Zhu, Limian Zhou, Yang Lu, Tingting Feng, Mengyu Dai, Jiaxue Liu, Wen Xu, Wanpeng Cheng, Fenfen Sun, Hua Liu, Wei Pan, Xiaoying Yang
Summary: Parasite-derived excretory-secretory products (ESPs) were found to alleviate cognitive impairment and gut microbiota dysbiosis induced by a high-fat diet, suggesting a potential therapeutic avenue for obesity-associated neurodegenerative diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Parasitology
Fabrizio Bruschi, Alisa Gruden-Movesijan, Barbara Pinto, Natasa Ilic, Ljiljana Sofronic-Milosavljevic
Summary: This study investigated the effects of products extracted from Trichinella spiralis muscle larvae on the experimental autoimmune encephalomyelitis (EAE) rat model, finding that treatment with ES L1 could alleviate EAE symptoms. Furthermore, lower levels of serum MMP-9 and NGAL/MMP-9 complex were observed in rats treated with ES L1, indicating a potential mechanism for the amelioration of EAE.
EXPERIMENTAL PARASITOLOGY
(2021)
Article
Parasitology
Yang Wang, Xue Bai, Bin Tang, Yulu Zhang, Lixiao Zhang, Xuepeng Cai, Jiaojiao Lin, Wanzhong Jia, Pascal Boireau, Mingyuan Liu, Xiaolei Liu
Summary: This study compared the differentially expressed proteins in the excretory-secretory products of different isolates of T. pseudospiralis muscle larvae, revealing their potential role in modulating host immune responses and maintaining a stable growth environment.
VETERINARY PARASITOLOGY
(2021)
Article
Parasitology
Jung-Woong Kim, Junyeong Yi, Jinhong Park, Ji Hoon Jeong, Jinho Kim, Jihee Won, Seok Chung, Tong-Soo Kim, Jhang Ho Pak
Summary: In this study, a three-dimensional in vitro cholangiocyte spheroid culture model was established to investigate the intrinsic pathological mechanisms of Clonorchis sinensis infection. Analysis of gene expression changes in ESP-treated cholangiocyte spheroids showed global alterations, with upregulated genes related to immune system processes, the extracellular region, and the extracellular matrix. The findings provide insights into the pathological pathways of liver fluke-associated hepatobiliary disorders and suggest potential therapeutic strategies for this infectious cancer.
PARASITES & VECTORS
(2021)
Article
Biochemistry & Molecular Biology
Kuang-Yao Chen, Yi-Ju Chen, Chien-Ju Cheng, Kai-Yuan Jhan, Cheng-Hsun Chiu, Lian-Chen Wang
Summary: 3-HBA and 4-HBA have been shown to increase cell viability and induce a protective phenotype in astrocytes in response to A. cantonensis young adults ESPs treatment by activating the Shh signaling pathway and inhibiting apoptosis-related molecule expression. These findings suggest that 3-HBA and 4-HBA may be potential therapeutic drugs for the treatment of human angiostrongyliasis.
BIOMEDICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ge Cheng, Zifang Zhang, Yixuan Wang, Youjiao Zao, Ruoqi Wang, Mengying Gao, Miaomiao Feng, Xi Zhang, Peng Jiang
Summary: Trichinella spiralis infection activates the TGF-beta 1/Smad3 pathway, promoting collagen secretion in muscle cells and playing an important role in the formation of collagen capsules. Further investigation is needed to identify the specific components in the Trichinella spiralis ES products that induce collagen synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Mengwei Zheng, Xuelian Jiang, Xinping Kong, Yanfeng Guo, Weiyu Zhang, Wenda Di
Summary: The study identifies the components of FgESP involved in the interaction between F. gigantica and buffaloes during different periods, and suggests their potential as vaccine and drug targets.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Huihui Li, Dapeng Qiu, Huijuan Yang, Yuan Yuan, Lingqin Wu, Liang Chu, Bin Zhan, Xiaoli Wang, Yan Sun, Wei Xu, Xiaodi Yang
Summary: Ts-AES has a therapeutic effect on sepsis-induced ALI by inhibiting pro-inflammatory cytokines and activating Tregs response. The expression of HMGB1, TLR2, and MyD88 in lung tissue was inhibited after treatment with Ts-AES. Ts-AES may exert immunomodulatory functions through the HMGB1/TLR2/MyD88 signaling pathway.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Aisha Munawar, Fakhar Zaman, Muhammad Waqas Ishaq, Khwaja Ali Hassan, Saima Masood, Zahid Ali, Khalid Abdul Majeed, Ahmed Akrem, Syed Abid Ali, Christian Betzel
Summary: This study compared the in vivo activity of four synthesized snake venom BPPs and found that their bioactivity varied. The sequences of peptide II and peptide IV can be used to improve the design of current ACE inhibitors used for hypertension treatment.
CURRENT MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Hannah Voss, Manuela Moritz, Penelope Pelczar, Nicola Gagliani, Samuel Huber, Vivien Nippert, Hartmut Schlueter, Jan Hahn
Summary: This study investigates the applicability of infrared laser tissue sampling for proteome analysis of different cell layers of murine intestine. By sampling directly from intact fresh-frozen tissue, the study successfully obtained the different proteomes of colon tissue's different cell layers. This new sampling method provides higher protein yield and identification compared to conventional mechanical homogenization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Yang Zhang, Benjamin Dreyer, Natalia Govorukhina, Alexander M. Heberle, Sasa Koncarevic, Christoph Krisp, Christiane A. . Opitz, Pauline Pfander, Rainer Bischoff, Hartmut Schlueter, Marcel Kwiatkowski, Kathrin Thedieck, Peter L. Horvatovich
Summary: The study compares three different quantitation techniques for tumor tissue phosphoproteomics and highlights the strengths and limitations of each method. The choice of quantitative methodology is critical for study design and comparison of published cancer phosphoproteomes. The results provide a resource for the design and analysis of quantitative phosphoproteomic studies in cancer research and diagnostics.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Hannah Voss, Simon Schlumbohm, Philip Barwikowski, Marcus Wurlitzer, Matthias Dottermusch, Philipp Neumann, Hartmut Schlueter, Julia E. Neumann, Christoph Krisp
Summary: HarmonizR is an efficient tool for missing data tolerant experimental variance reduction, which does not require data imputation and can be easily adjusted for individual dataset properties and user preferences. It demonstrated successful data harmonization for different tissue preservation techniques, LC-MS/MS instrumentation setups, and quantification approaches, and outperformed data imputation methods in detecting significant proteins.
NATURE COMMUNICATIONS
(2022)
Correction
Biochemical Research Methods
Yudong Guan, Min Zhang, Manasi Gaikwad, Hannah Voss, Ramin Fazel, Samira Ansari, Huali Shen, Jigang Wang, Hartmut Schluter
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Raphael J. Eberle, Marc Sevenich, Ian Gering, Lara Scharbert, Birgit Strodel, Nils A. . Lakomek, Karoline Santur, Jeannine Mohrlueder, Monika A. . Coronado, Dieter Willbold
Summary: A novel and efficient next-generation sequencing (NGS) supported phage display selection strategy was used to identify a set of peptide ligands that inhibit the SARS-CoV-2 3CL protease. These ligands have the potential to be lead compounds for therapeutic development and address limitations associated with current L-peptide inhibitors.
ACS CHEMICAL BIOLOGY
(2023)
Article
Oncology
Sama Rezasoltani, Hamid Asadzadeh Aghdaei, Seyedesomaye Jasemi, Maria Gazouli, Nikolas Dovrolis, Amir Sadeghi, Hartmut Schlueter, Mohammad Reza Zali, Leonardo Antonio Sechi, Mohammad Mehdi Feizabadi
Summary: This study conducted a comprehensive analysis of the microbiome in saliva and stool samples of colorectal cancer (CRC) patients in Iran, comparing them with healthy controls. The results showed distinct clustering of microbial genera in CRC patients and healthy controls, indicating unique microbial signatures in both conditions. However, further investigation is needed to understand the roles of microbiota in CRC and healthy individuals.
Article
Biochemistry & Molecular Biology
Eva B. Znalesniak, Aikaterini Laskou, Franz Salm, Katharina Haupenthal, Soenke Harder, Hartmut Schlueter, Werner Hoffmann
Summary: TFF2 is a protein that is co-secreted with MUC6 in the gastric mucous neck cells, antral gland cells, and duodenal Brunner glands. It forms a high-molecular-mass complex with MUC6, stabilizing the mucus barrier. In the pancreas, TFF2 exists as a low-molecular-mass monomer. Its absence in the pancreas is due to a lack of MUC6. Tff2 plays a protective role in the pancreatic ductal epithelium.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Rupert Stadlhofer, Manuela Moritz, Marceline M. Fuh, Jorg Heeren, Henrike Zech, Till S. Clauditz, Hartmut Schlueter, Christian S. Betz, Dennis Eggert, Arne Boettcher, Jan Hahn
Summary: Nanosecond infrared laser tissue sampling combined with mass spectrometry lipidomic analysis is a feasible and promising approach for differentiating oropharyngeal squamous cell carcinoma from non-tumorous oropharyngeal tissue.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Monika A. Coronado, Ian Gering, Marc Sevenich, Danilo S. Olivier, Mohammadamin Mastalipour, Marcos S. Amaral, Dieter Willbold, Raphael J. Eberle
Summary: Epigallocatechin molecules, found in green tea, have shown great potential in inhibiting flaviviruses such as Dengue, yellow fever, West Nile, and Zika. This discovery could lead to the development of more effective antiviral drugs.
Article
Microbiology
Archile Paguem, Kingsley Manchang, Pierre Kamtsap, Alfons Renz, Sabine Schaper, Gerhard Dobler, Deon K. Bakkes, Lidia Chitimia-Dobler
Summary: This study investigates the diversity of ticks and Rickettsia species parasitizing wild animals sold in bush meat markets in Cameroon. It identifies 18 tick species belonging to five genera and detects two Rickettsia spp. The findings highlight the high diversity of ticks among wildlife in Cameroon.
Article
Biochemistry & Molecular Biology
Franz Salm, Eva B. Znalesniak, Aikaterini Laskou, Sonke Harder, Hartmut Schlueter, Werner Hoffmann
Summary: Tff1 is a gastric peptide secreted with mucin, Muc5ac, and Tff1KO mice serve as a recognized model for antral tumorigenesis. The comparison of gene expression between Tff1KO mice and their wild-type littermates reveals the presence of different mucosal protection systems in the proximal colon and/or duodenum. On the protein level, various forms of Tff1 and a high-molecular-mass Tff2/Muc6 complex were identified in the duodenum and proximal colon respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Siti Nurul Hidayah, Ali Biabani, Manasi Gaikwad, Paula Nissen, Hannah Voss, Maria Riedner, Hartmut Schlueter, Bente Siebels
Summary: Fractionation of proteoforms is a challenging topic in proteoform analysis. Considering the existence of proteoforms in experimental approaches is crucial in Life Science research and the manufacturing of therapeutic proteins. This study demonstrates that sample displacement batch chromatography is an easy-to-handle, economical, and efficient method for fractionating proteoforms.
Article
Cell Biology
Devon Siemes, Pieter Vancamp, Boyka Markova, Philippa Spangenberg, Olga Shevchuk, Bente Siebels, Hartmut Schlueter, Steffen Mayerl, Heike Heuer, Daniel Robert Engel
Summary: In this study, the researchers investigated the mechanisms underlying locomotor disabilities caused by thyroid hormone transporter MCT8 deficiency using an animal model. They performed proteome analysis and found significant differences in protein expression between wild-type and double knockout mice. They also identified a potential candidate gene, Pde10a, which may be involved in the pathogenic pathway of human MCT8 deficiency.
Article
Chemistry, Analytical
Jelena Navolicc, Manuela Moritz, Hannah Voss, Simon Schlumbohm, Yannis Schumann, Hartmut Schluter, Julia E. Neumann, Jan Hahn
Summary: Common workflows in bottom-up proteomics often fail to represent rare cell types due to the homogenization of tissue samples. In this study, a new 3D tissue sampling and homogenization method utilizing nanosecond infrared laser ablation was proposed. The method allows for better representation of sample composition and spatial proteome signatures, and was successfully used to analyze embryonic mouse heads.
ANALYTICAL CHEMISTRY
(2023)
Article
Parasitology
Gabriela Martinez Moura Tavares, Hannar Angelica de Melo Alverga, Estela Moraes Felix, Giovana de Oliveira Dichman, Gabriel Monteiro Pinheiro, Yara Juliano, Priscila Paruci, Dennis Minoru Fujita, Lucas Melo Neves, Marina Tiemi Shio, Luiz Henrique da Silva Nali
Summary: This study investigated the frequency of N. gonorrhoeae infection in women in a poor area of São Paulo and found that most of the strains detected showed resistance to one or more antimicrobials.
Article
Parasitology
Qian Yao, Ying-Ying Fan, Shuang Huang, Gui-Rong Hu, Jun-Ke Song, Xin Yang, Guang-Hui Zhao
Summary: This study found that the expression of miR-4521 was upregulated during C. parvum infection, and this upregulation was related to the TLR/NF-kappa B signal pathway. Additionally, miR-4521 promoted the propagation of C. parvum in HCT-8 cells by regulating BCL2-mediated cell apoptosis through targeting foxm1.
Article
Parasitology
Cho Naing, Maxine A. Whittaker, Wong Siew Tung, Htar Aung, Joon Wah Mak
Summary: This study aimed to estimate the prevalence of zoonotic brugian filariasis in animals and compare it with the prevalence in humans in the same area. The findings suggest that cats, dogs, and leaf-eating monkeys may serve as potential reservoirs for human brugian filariasis.
Review
Parasitology
Raissa Nogueira de Brito, Susan Tanner, Julie Velasquez Runk, Juliana Hoyos
Summary: This study conducted a scoping review to identify major research themes and knowledge gaps in the social science literature on leishmaniases or Chagas disease. The findings showed that research in this field mainly focuses on the sociocultural, economic, historical, and political dimensions of the diseases. Future research should pay more attention to how social institutions and economic factors shape disease education, control measures, healthcare access, and quality of life of affected individuals.
Article
Parasitology
Minghao Zheng, Mengyao Zhang, Hao Li, Shipo Wu, Yuee Zhao, Jinlong Zhang, Yunyue Zhou, Mohamed Boie Jalloh, Kun Zhang, Lina Chen, Zhiqiang Mi, Yong Cui, Lihua Hou
Summary: Rapid and convenient detection of Plasmodium falciparum, the deadliest malaria parasite in Africa, is crucial for malaria eradication efforts in medical resource-limited settings. A CRISPR-based diagnostic method that can detect P. falciparum without nucleic acid extraction was developed and showed high sensitivity and consistency with qPCR test. It can also detect artemisinin-resistant strains and other laboratory-cultured strains. This method has great potential for clinical testing and large-scale community screening in Africa and other regions.
Article
Parasitology
Fabian Correa-Morales, Cassandra Gonzalez-Acosta, David Ibarra-Ojeda, Miguel Moreno-Garcia
Summary: Since 2002, West Nile Virus (WNV) has been reported in 18 states in Mexico. This paper discusses the proposed hypotheses for the absence of WNV outbreaks in Latin America and provides a context-specific explanation for Mexico.
Article
Parasitology
Umberto Molini, Lauren M. Coetzee, Vernon Christians, Maria Y. Hemberger, Bernard Chiwome, Maria Amukwaya, Siegfried Khaiseb, Giovanni Cattoli, William G. Dundon, Giovanni Franzo
Summary: Porcine circovirus 3 (PCV-3) has been reported globally since its first identification in 2015. Limited data on PCV-3 in Africa led to a study in Namibia, which found a high prevalence of PCV-3 in backyard piggeries, but not in commercial farms with higher biosecurity measures. The source of viral introduction and factors such as semen importation, feeding backyard pigs with human food waste, pig exchanges, and wildlife movements may be involved in the dispersal of PCV-3 within Namibia. Further studies are needed to understand the epidemiology and impact of PCV-3 on pig productivity and wildlife health.
Article
Parasitology
Chutchai Piewbang, Panida Poonsin, Pattiya Lohavicharn, Panitnan Punyathi, Sawang Kesdangsakonwut, Tanit Kasantikul, Somporn Techangamsuwan
Summary: This study provides insights into the pathology of natural SARS-CoV-2 infection in dogs, revealing severe damage to the lungs and involvement of multiple organs. The findings suggest the potential utility of dogs as a model for studying SARS-CoV-2 infection in humans, highlighting the need for further investigation.
Article
Parasitology
Julian Avila-Jimenez, Juan David Gutierrez, Mariano Altamiranda-Saavedra
Summary: Leishmaniasis is a parasitic disease transmitted to humans by sandfly vectors and vertebrate hosts. This study used ecological niche modeling to investigate the potential distribution of host and vector species associated with cutaneous leishmaniasis transmission, revealing different distribution patterns under varying climatic conditions.
Article
Parasitology
Luis A. Gomez-Puerta, Juan Olazabal, Santiago Paredes, Juan Lopez, Miguel Anampa, Angelica Mondragon, Ana Vargas-Calla, Hugo Castillo
Summary: This study reports the first detection of Candidatus M. haemolamae infection in vicunas from the Pampa Galeras National Reserve in Peru, using molecular diagnosis. Tick-infested vicunas had a higher likelihood of being infected with Candidatus M. haemolamae compared to tick-free vicunas. Gender and age were not associated with infection.
Article
Parasitology
Berlin Londono-Renteria, Zakaria Seidu, Helena Lamptey, Michael F. Ofori, Lars Hviid, Mary Lopez-Perez
Summary: Heterozygous carriers of HbAS and HbAC have a reduced risk of severe malaria, but not immunity against Plasmodium falciparum infection. Antibody levels against gSG6-P1 peptide in Anopheles mosquito saliva can serve as a serological biomarker for exposure to Plasmodium infection. This study highlights the importance of studying the role of hemoglobinopathies in malaria transmission for improving control interventions.
Article
Parasitology
Imane Belbacha, Meryem El Azzouzi, Rajae Bensghir, Kamal Filali Marhoum, Khadija Hajjout, El Mir Elharti, Khalid Sadki, Hicham Oumzil
Summary: This study investigates the association of three SNPs in the APOBEC3G gene with disease outcomes in Moroccan HIV-1 infected patients. The rs2294367 CG genotype is strongly associated with protection against HIV-1 infection, particularly in older individuals.
Article
Parasitology
Tan Luong, Do Kien Tran, Anh Hung Pham, Thi Thu-Ha Hoang, Van Khang Pham, Quang Thai Pham, Thi Mai Hung Tran, Minh Hoa Luong, Thanh Long Pham, Jason K. Blackburn
Summary: This study examined the epidemiology of anthrax in Lai Chau province, Vietnam, using historical data. Spatial clusters of human and livestock anthrax were identified, and the characteristics of the disease were described. The study found that there were distinct differences between the high-risk areas for human and livestock anthrax, and most human cases occurred during specific seasons. Additionally, the decrease in livestock anthrax vaccine coverage was associated with an increase in disease incidence.
Article
Parasitology
Jin-tao Chen, Jian-bo Zhan, Ming-chao Zhu, Kai-ji Li, Man-qing Liu, Bin Hu, Kun Cai, Hai-rong Xiong, Shu-liang Chen, Wei-long Tan, Liang-jun Chen, Wei Hou
Summary: This study investigated the prevalence and variation of orthohantavirus in rodents and humans in Hubei province, China. It identified the diversity of orthohantaviruses circulating in the region, with the HV004-like subtype being the main genotype in rodents and patients. This highlights the importance of continued attention and research on orthohantaviruses, especially newly identified strains.
