Review
Biotechnology & Applied Microbiology
Wendy J. Lyzenga, Curtis J. Pozniak, Sateesh Kagale
Summary: Human population growth has increased the demand for food crops and biomaterials, while climate change is impacting environmental conditions. Therefore, there is a need to develop crop varieties that can tolerate adverse growth conditions while requiring fewer inputs. CRISPR/Cas technology has great potential in plant breeding.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Genetics & Heredity
Gauri Nerkar, Suman Devarumath, Madhavi Purankar, Atul Kumar, R. Valarmathi, Rachayya Devarumath, C. Appunu
Summary: The challenges posed by global climate change and various unfavorable factors in agriculture have led to the emergence of genome editing technology, which provides a new approach to improving crop productivity. The technology enables precise modification of crop genomes, accelerating the breeding process and enhancing efficiency.
FRONTIERS IN GENETICS
(2022)
Review
Agronomy
Xuehan Xia, Xinhua Cheng, Rui Li, Juanni Yao, Zhengguo Li, Yulin Cheng
Summary: Genome editing, represented by the CRISPR/Cas9 system, has been widely used in plants for improving fruit yield and quality, increasing stress resistance, accelerating the domestication of wild tomato, and customizing tomato cultivars for urban agriculture. Tomato, as an important applied plant, serves as an excellent model plant for genome editing research.
THEORETICAL AND APPLIED GENETICS
(2021)
Review
Biotechnology & Applied Microbiology
Tien Van Vu, Duong Thi Hai Doan, Jihae Kim, Yeon Woo Sung, Mil Thi Tran, Young Jong Song, Swati Das, Jae-Yean Kim
Summary: The ultimate goals of genetic engineering are to achieve gene editing and allele introgression with absolute precision and control. Various approaches have been developed in plants for precision genome editing, with significant advancements in HR-mediated editing frequencies through CRISPR/Cas technology. Despite this progress, further research is needed for commercially viable applications of precise genome editing, potentially requiring alternative innovative methods.
PLANT BIOTECHNOLOGY JOURNAL
(2021)
Review
Biology
Robert W. A. Potts, Alejandro P. Gutierrez, Carolina S. Penaloza, Tim Regan, Tim P. Bean, Ross D. Houston
Summary: Molluscan aquaculture is important for global seafood production, but faces challenges from infectious disease outbreaks. Selective breeding, genomic technologies, and genome editing show promise in improving genetic resistance to disease in molluscs, with tailored approaches needed due to their unique characteristics.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2021)
Review
Plant Sciences
Satoru Sukegawa, Hiroaki Saika, Seiichi Toki
Summary: The development of CRISPR/Cas9 system has revolutionized biology by enabling targeted mutagenesis, gene targeting, and chromosome engineering. Genome-editing technologies have advanced rapidly, allowing precise gene modifications and rearrangement of genomes and chromosomes in plants.
Review
Plant Sciences
Silvia Farinati, Samela Draga, Angelo Betto, Fabio Palumbo, Alessandro Vannozzi, Margherita Lucchin, Gianni Barcaccia
Summary: Plant male sterility refers to the inability of plants to produce functional anthers, pollen, or male gametes. Developing male sterility lines is crucial in plant breeding programs, especially for F1 hybrid production. Various techniques, ranging from conventional breeding methods to advanced genome editing technologies such as CRISPR/Cas-based tools, have been used to achieve male sterility. CRISPR/Cas-mediated gene knockout has emerged as a promising and efficient method for altering gene function and inducing male sterility. This article provides an overview of plant male sterility and focuses on the recent advancements and applications of CRISPR-based breeding techniques for inducing male sterility and introducing desirable alleles.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Tian Min, Delight Hwarari, Dong'ao Li, Ali Movahedi, Liming Yang
Summary: CRISPR/Cas-based genome editing technology has significant potential in woody plants for gene function research, germplasm innovation, and genetic improvement. However, the complexity of woody plant genomes may pose challenges for the application and expansion of this technology. Therefore, the development of molecular breeding research based on woody plants is of great importance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Raed Ibraheim, Phillip W. L. Tai, Aamir Mir, Nida Javeed, Jiaming Wang, Tomas C. Rodriguez, Suk Namkung, Samantha Nelson, Eraj Shafiq Khokhar, Esther Mintzer, Stacy Maitland, Zexiang Chen, Yueying Cao, Emmanouela Tsagkaraki, Scot A. Wolfe, Dan Wang, Athma A. Pai, Wen Xue, Guangping Gao, Erik J. Sontheimer
Summary: This study introduces a single-vector AAV platform for effective genome editing therapy, along with a nanopore-based sequencing platform for quality control. The results demonstrate successful treatment of two genetic disease models in mice using these platforms.
NATURE COMMUNICATIONS
(2021)
Review
Immunology
Alexander Biederstaedt, Gohar Shahwar Manzar, May Daher
Summary: The advent of cellular immunotherapy and precision genome editing has revolutionized cancer treatment, offering new possibilities but also facing certain limitations. Various engineering strategies are being increasingly utilized to overcome present constraints in building more potent cellular therapeutics.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Daniela Marone, Anna Maria Mastrangelo, Grazia Maria Borrelli
Summary: Biotechnological approaches such as transgenesis and genome editing have become increasingly important for genetic improvement of crops. These approaches have led to the development of traits such as resistance to herbicides and insects, as well as traits related to human population growth and climate change. However, the use of these approaches is limited by legislative restrictions and ongoing public debate. This review provides an updated and in-depth discussion on these issues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Fazal Rehman, Haiguang Gong, Yufei Bao, Shaohua Zeng, Hongwen Huang, Ying Wang
Summary: This article provides an overview of the application and impact of CRISPR technology in Solanaceae crops. By discussing domestication traits and describing molecular mechanisms, it reveals the great potential of CRISPR gene editing in breeding Solanaceae crops. Additionally, it raises questions about the future prospects and regulatory risks of CRISPR technology in breeding minor Solanaceae crops with significant industrial value.
PLANT MOLECULAR BIOLOGY
(2022)
Review
Plant Sciences
Junming Zhou, Xinchao Luan, Yixuan Liu, Lixue Wang, Jiaxin Wang, Songnan Yang, Shuying Liu, Jun Zhang, Huijing Liu, Dan Yao
Summary: CRISPR-Cas9 gene editing technology is widely used for crop improvement due to its simplicity, high efficiency, specificity, and low production costs. Improving the gene-editing efficiency is crucial for molecular breeding, and this review discusses strategies such as Cas9 variant enzyme engineering, the use of multiple promoter-driven Cas9, and efficient optimization and expression of gRNA. It also briefly introduces optimization strategies for the CRISPR/Cas12a system and the application of precision editing.
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Plant Sciences
Olga Kershanskaya, Gulvira L. Yessenbaeva, Darya S. Nelidova, Aizhan N. Karabekova, Zarina N. Sadullaeva
Summary: The CRISPR/Cas9 technology has the potential to improve crop breeding efficiency and antiviral defense, and its application in barley breeding has shown promising results.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Reproductive Biology
R. Nunez-Olivera, F. Cuadro, D. Bosolasco, V de Brun, J. de la Mata, C. Brochado, A. Meikle, G. A. Bo, A. Menchaca
Article
Agriculture, Dairy & Animal Science
Maria Noel Meikle, Ana Paula Arevalo, Geraldine Schlapp, Gabriel Fernandez-Grana, Alejo Menchaca, Martina Crispo
Article
Agriculture, Dairy & Animal Science
G. M. Braganca, R. I. T. P. Batista, J. M. G. Souza-Fabjan, V. A. P. Alfradique, E. K. N. Arashiro, P. H. N. Pinto, J. D. R. Santos, L. S. A. Camargo, A. Menchaca, J. F. da Fonseca, F. Z. Brandao
Summary: This study demonstrated that progesterone and medroxyprogesterone acetate had different effects on the transcriptome profiling of immature cumulus-oocyte complexes in sheep, with exogenous progesterone positively impacting the expression of genes related to oocyte quality.
DOMESTIC ANIMAL ENDOCRINOLOGY
(2021)
Article
Reproductive Biology
M. Crispo, M. N. Meikle, G. Schlapp, A. Menchaca
Summary: This study demonstrates comparable superovulatory response and embryo development between recombinant and natural eCG treatment in mice. The results show that reCG treatment yields similar development outcomes to natural eCG, with better results than females not receiving eCG treatment.
Article
Reproductive Biology
Andres V. Cedeno, Rodrigo Cuervo, Andes Tribulo, Ricardo Tribulo, Salvador Andrada, Reuben Mapletoft, Alejo Menchaca, Gabriel A. Bo
Summary: The three experiments showed that expression of estrus and GnRH treatment significantly affected pregnancy rates in beef cattle. Cows that exhibited estrus had earlier ovulations and higher pregnancy rates, while GnRH treatment in animals that did not show estrus accelerated ovulation and increased pregnancy rates.
Article
Reproductive Biology
D. Bosolasco, R. Nunez-Olivera, V. de Brun, A. Meikle, A. Menchaca
Summary: Administering estradiol cypionate at progesterone insert removal can increase serum estradiol concentrations, enhance ovulation, and improve uterine function in postpartum anestrous beef cows. The dose of estradiol cypionate affects the timing of ovulation and the size of the ovulatory follicle.
Article
Agriculture, Dairy & Animal Science
A. Menchaca, D. Bosolasco, R. Nunez-Olivera, F. Cuadro, C. J. M. Speciale, S. Perez-Wallace
Summary: This study evaluated the interaction between estradiol cypionate dose and ovarian status in beef cows under different weaning/suckling regimens before fixed-time artificial insemination. The results showed that the dose of ECP had differential effects on pregnancy per artificial insemination in early-weaned and suckled cows. Overall, the administration of ECP affected fertility in a dose-dependent manner, indicating an interaction between suckling and estrous cycling effects.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Veterinary Sciences
Camila Garcia-Pintos, Franklin Riet-Correa, Alejo Menchaca
Summary: This study found that foot-and-mouth disease (FMD) vaccine administered before 45 days of gestation increases pregnancy failure in Bos taurus beef cows, which is associated with hyperthermia and local adverse reactions. However, no effect on pregnancy failure was observed when the vaccine was administered after 45 days of gestation.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Reproductive Biology
R. Nunez-Olivera, G. A. Bo, A. Menchaca
Summary: This study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate. The results showed that extending proestrus length could increase the diameter of the preovulatory follicle, proportion of heifers expressing estrus, and pregnancy rate in beef heifers.
Article
Reproductive Biology
Juan Carlos Tschopp, Alejandro J. Macagno, Reuben J. Mapletoft, Alejo Menchaca, Gabriel A. Bo
Summary: Two experiments were conducted to determine the effect of adding GnRH at the beginning of a synchronization protocol and/or a second dose of PGF2 alpha the day before P4 device removal on the pregnancy rate in lactating dairy cows. The results showed that the addition of GnRH on Day 0 and a second dose of PGF2 alpha improved the pregnancy rate in cows synchronized with an estradiol/P4-based protocol.
Article
Biochemistry & Molecular Biology
Rossina Novas, Tatiana Basika, Megan E. Williamson, Pablo Fresia, Alejo Menchaca, Maxwell J. Scott
Summary: The New World screwworm, a major pest of livestock, was successfully eradicated from North and Central America using the sterile insect technique. However, more cost-effective genetic methods may be needed to control this parasite in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide an efficient means to suppress screwworm populations.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Reproductive Biology
P. C. dos Santos-Neto, F. Cuadro, M. Souza-Neves, M. Crispo, A. Menchaca
Summary: In this study, the developmental competence, pregnancy outcomes, and embryo survival and birth rate of CRISPR/Cas microinjected zygotes in large scale programs in sheep were evaluated. The results showed that the developmental rate of microinjected zygotes was lower than non-injected zygotes, but similar outcomes were achieved when the embryos were transferred into the uterine horn instead of the oviduct. Furthermore, vitrification of CRISPR/Cas microinjected embryos did not significantly affect pregnancy rate, embryo survival rate, and birth rate.
Review
Agriculture, Dairy & Animal Science
Isabel Oliveira Cosentino, Mario Felipe Alvarez Balaro, Alejo Menchaca, Raquel Perez-Clariget, Rodolfo Ungerfeld, Felipe Zandonadi Brandao
Summary: Hormonal methodologies are widely used and evolved to control small ruminants' estrous cycle, aiming to optimize reproductive performance. The induced and/or synchronized estrous cycle can be utilized for fixed-time artificial insemination or based on estrus behavior signs for natural or guided mating. Resynchronization protocols have been developed to increase reproductive outcomes in females that failed to conceive, aiming to resynchronize ovulation as early as non-pregnancy is detected. This review provides a summary of recent advances and main findings regarding resynchronization protocols in small ruminants, as well as future perspectives and new research directions. Although the field of resynchronization treatment in small ruminant reproduction is still developing, enhancements in reproductive outcomes indicate that these protocols can be successfully utilized in sheep and goat production.
ANIMAL REPRODUCTION
(2023)