Article
Pharmacology & Pharmacy
Wei Liu, Yanghui Ou, Yumeng Yang, Xuemei Zhang, Liqi Huang, Xiaohua Wang, Buling Wu, Mingcheng Huang
Summary: The study demonstrated that punicalagin has a protective effect on HUVECs activation through an endothelial-mediated mechanism, which may be used in the treatment of various disorders such as cancer, rheumatoid arthritis, and cardiovascular disease. This effect is achieved by reducing adhesion of monocyte cells to HUVECs, decreasing expression of ICAM-1 and VCAM-1, and inhibiting permeability, proliferation, migration, and tube formation in VEGF-induced HUVECs. Additionally, punicalagin suppresses IKK-mediated activation of NF-kappa B signaling and inhibits VEGFR2 activation and downstream p-PAK1.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biology
Nur Syakirah Othman, Amilia Aminuddin, Shahidee Zainal Abidin, Saiful Effendi Syafruddin, Mohd Faizal Ahmad, Norfilza Mohd Mokhtar, Jaya Kumar, Adila A. Hamid, Azizah Ugusman
Summary: Hyperglycemia in diabetes mellitus leads to oxidative stress, apoptosis, and dysfunction of vascular endothelial cells. This study aimed to analyze the miRNA profile of human umbilical-vein endothelial cells (HUVECs) exposed to hyperglycemia. RNA sequencing revealed 17 differentially expressed miRNAs between control and hyperglycemia groups, with 4 upregulated and 13 downregulated miRNAs. Validation with qPCR confirmed the differential expression of two miRNAs. These findings suggest a role for miRNAs in diabetic vascular endothelial dysfunction and provide potential targets for therapy.
Article
Oncology
Jichong Xu, Hao Feng, Lin Ma, Huaqiao Tan, Shuo Yan, Chun Fang
Summary: The study found that Bak-IIIa alleviated LPS-induced inflammatory damage by upregulating LINC00294, demonstrating its potential for preventing vascular inflammation.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Food Science & Technology
Ho-Jeong Bang, Mok-Ryeon Ahn
Summary: This study found that pinobanksin exhibits antiangiogenic effect by inducing endothelial cell apoptosis, and it can be developed as an antiangiogenic agent. The mechanism involves inactivation of the Raf/MEK/ERK pathway and activation of apoptotic signaling such as caspase cascades.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Environmental Sciences
Jiajing Cui, Pingwei Wang, Shuqi Yan, Yujun Liang, Dongge Liu, Shuping Ren
Summary: The study aims to explore the role of ferroptosis in endothelial cell dysfunction and its underlying mechanisms.
Article
Biochemistry & Molecular Biology
Kejia Kan, Yifei Mu, Marielle Bouschbacher, Carsten Sticht, Natalia Kuch, Martin Sigl, Nuh Rahbari, Norbert Gretz, Prama Pallavi, Michael Keese
Summary: The study revealed a novel biphasic response of human umbilical vein endothelial cells to blue light illumination. Low fluence blue light promoted cell activities, while high fluence illumination had the opposite effect by upregulating pro-apoptotic signaling pathways. These findings provide insights into photobiomodulation by blue light and potential treatment strategies for angiogenesis-dependent diseases.
Article
Chemistry, Medicinal
Chin-Feng Hsuan, Thung-Lip Lee, Wei-Kung Tseng, Chau-Chung Wu, Chi-Chang Chang, Tsui-Ling Ko, Ya-Ling Chen, Jer-Yiing Houng
Summary: The study demonstrated that extracts from Glossogyne tenuifolia (GT) can significantly increase nitric oxide (NO) production in human umbilical vein endothelial cells (HUVECs), with the root methanol extract showing the most potent effect. The root methanol extract increased NO production by increasing eNOS expression and activating eNOS phosphorylation at serine(1177).
Article
Oncology
Jiaqiong Lin, Xiaoyong Li, Yan Lin, Zena Huang, Wen Wu
Summary: Hydrogen sulfide has demonstrated potential in treating various diseases by protecting blood vessels. This study showed that exogenous H2S can inhibit high glucose-induced injuries and inflammation, suggesting it may be an effective drug for treating diabetic vascular complications.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Carina A. Valenzuela, Ella J. Baker, Elizabeth A. Miles, Philip C. Calder
Summary: Conjugated linoleic acid (CLA) isomers, specifically cis-9, trans-11 (CLA9,11) and trans-10, cis-12 (CLA10,12), have shown modest anti-inflammatory effects on endothelial cells (ECs) at low concentrations. These CLAs decrease the concentrations of inflammatory factors such as MCP-1, IL-6, IL-8, and RANTES in the culture medium, but also upregulate some pro-inflammatory genes while downregulating others. The findings suggest that CLAs may have a role in preventing atherosclerosis by modulating inflammation in ECs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Shouzhu Xu, Yu Yan, Zhijiao Yan, Jie Xu, Baoning Qi, Juan Li, Zhigang Zhang, Yuanping Han, Jing Zhao
Summary: The study revealed that treatment with septic serum led to significant decrease in HUVEC viability, alterations in cell structure, and increased secretion of inflammatory cytokines. Furthermore, septic serum induced increased generation of ROS, activation of intracellular signaling kinases, and translocation of NF-kappa B.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lukasz Gwozdzinski, Joanna Bernasinska-Slomczewska, Pawel Hikisz, Anna Wiktorowska-Owczarek, Edward Kowalczyk, Anna Pieniazek
Summary: In this study, the properties of human varicose vein endothelial cells (HVVEC) and human umbilical vein endothelial cells (HUVEC) were compared. Three bioactive compounds, diosmin, escin, and bromelain, were tested on the cells. The drugs showed different effects on reactive oxygen species and nitric oxide generation in the cells. The study revealed a potential mechanism of action for VV therapy drugs on HVVEC cells.
Article
Genetics & Heredity
Regina L. Binz, Rupak Pathak
Summary: This study investigated the cytogenetic integrity of primary human umbilical vein endothelial cells (HUVECs) and found that approximately 50% of the cells have a deletion of the Xq terminal region. Additionally, a similar Xq deletion was identified in the daughter cell line generated by fusing HUVECs with A549 cells.
Article
Environmental Sciences
Zixuan Liu, Xiaomin Xia, Xuying Lv, Erqun Song, Yang Song
Summary: Iron-bearing nanoparticles (IBNPs) are abundant in particulate matter (PM) and considered hazardous to human health with unclear toxic mode-of-action. Studies show that IBNPs promote ferroptosis, affecting intracellular iron levels, disrupting cell signaling, causing mitochondrial dysregulation, and activating related genes. This highlights the importance of recognizing the ferroptotic activity of IBNPs for assessing the health risks associated with PM.
ENVIRONMENTAL POLLUTION
(2021)
Article
Nutrition & Dietetics
Ruihao Wu, Jiayin Zhong, Lei Song, Min Zhang, Lulu Chen, Li Zhang, Zhaohui Qiu
Summary: In this study, untargeted metabolomics was used to examine differentially expressed metabolites of vascular endothelial cells, aiming to understand the metabolic regulation of ischemic injury. Various methods including CCK8 detection, flow cytometry, ROS detection, JC-1 detection, and western blotting were employed to assess the effects of differentially expressed metabolites on apoptosis and oxidative stress in cells. The impacted metabolic pathways were further confirmed by western blotting and RT-PCR.
NUTRITION & METABOLISM
(2023)
Article
Cardiac & Cardiovascular Systems
Mengqi Gu, Fengyuan Zhang, Xiaotong Jiang, Pengzheng Chen, Shuting Wan, Qingfeng Lv, Yuan Lu, Qian Zhou, Yanyun Wang, Lei Li
Summary: This study aimed to evaluate the effects of placental exosomes on endothelial cells. The results showed that H/R-exo and PE-exo inhibited the biological functions of endothelial cells and impaired vessel development, potentially leading to vascular disorders in offspring.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Nadia Di Pietrantonio, Carola Palmerini, Caterina Pipino, Maria Pompea Antonia Baldassarre, Giuseppina Bologna, Angelika Mohn, Cosimo Giannini, Paola Lanuti, Francesco Chiarelli, Assunta Pandolfi, Natalia Di Pietro
Summary: Childhood obesity is associated with impaired vascular insulin sensitivity, altered oxidant-antioxidant state, and chronic inflammation leading to endothelial dysfunction. In vitro study shows that inhibiting the mTORC1-S6K1 pathway can restore insulin receptor signaling and provide insights into the mechanisms underlying endothelial dysfunction in childhood obesity.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Carola Palmerini, Luca Piscitani, Giuseppina Bologna, Chiara Riganti, Paola Lanuti, Domitilla Mandatori, Lorenzo Di Liberato, Giorgia Di Fulvio, Vittorio Sirolli, Giulia Renda, Caterina Pipino, Marco Marchisio, Mario Bonomini, Assunta Pandolfi, Natalia Di Pietro
Summary: In chronic kidney disease patients, hemodialysis (HD) and conservative therapy (CT) result in accumulation of cGMP in red blood cells, while peritoneal dialysis (PD) may better preserve the synthetic NO pathway. Changes in plasma cGMP levels by altering the activity of cGMP membrane transporter were observed in HD and CT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Nutrition & Dietetics
Domitilla Mandatori, Letizia Pelusi, Valeria Schiavone, Caterina Pipino, Natalia Di Pietro, Assunta Pandolfi
Summary: Osteoporosis and vascular calcification are common health issues in the elderly population, with recent studies suggesting a direct correlation between the two; Vitamin K2 plays a crucial role in maintaining calcium balance, and its deficiency may lead to the calcium paradox phenomenon; The VitK2 family has been proposed as natural bioactive molecules that could potentially prevent bone loss and vascular calcification.
Article
Urology & Nephrology
Eiichiro Satake, Pierre-Jean Saulnier, Hiroki Kobayashi, Manoj K. Gupta, Helen C. Looker, Jonathan M. Wilson, Zaipul I. Md Dom, Katsuhito Ihara, Kristina O'Neil, Bozena Krolewski, Caterina Pipino, Meda E. Pavkov, Viji Nair, Markus Bitzer, Monika A. Niewczas, Matthias Kretzler, Michael Mauer, Alessandro Doria, Behzad Najafian, Rohit N. Kulkarni, Kevin L. Duffin, Marcus G. Pezzolesi, C. Ronald Kahn, Robert G. Nelson, Andrzej S. Krolewski
Summary: This study investigated the mechanisms involved in the progression of diabetic kidney disease to ESKD through global miRNA analysis and proteomics analysis. Circulating miRNAs and proteins in the axon guidance pathway were found to be strongly associated with the risk of ESKD development. These findings suggest that systemic factors play an important role in the development of diabetic kidney disease and may serve as potential targets for new therapies.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Article
Chemistry, Physical
Francesco De Angelis, Domitilla Mandatori, Valeria Schiavone, Francesco Paolo Melito, Silvia Valentinuzzi, Mirco Vadini, Pamela Di Tomo, Lorenzo Vanini, Letizia Pelusi, Caterina Pipino, Piero Del Boccio, Camillo D'Arcangelo, Assunta Pandolfi
Summary: The study aimed to evaluate the cytotoxic and genotoxic potential of five commercially available dental composite resins, finding that two of them significantly affected cellular metabolic activity and genotoxicity. While not all resin extracts induced detectable oxidative stress, only two resins showed significant genotoxicity.
Article
Nutrition & Dietetics
Domitilla Mandatori, Letizia Penolazzi, Letizia Pelusi, Elisabetta Lambertini, Francesca Michelucci, Annamaria Porreca, Pietro Cerritelli, Caterina Pipino, Angelo Di Iorio, Danilo Bruni, Marta Di Nicola, Roberto Buda, Roberta Piva, Assunta Pandolfi
Summary: Researchers have developed a three-dimensional cell culture system closer to the bone microenvironment by co-culturing osteoblasts and osteoclast precursors, aiming to better understand the effects of vitamin K2 on bone metabolism. They found that this method was more informative in analyzing responsiveness to vitamin K2 and could potentially be used to predict individual responsiveness to molecules designed to positively impact bone health.
Article
Nutrition & Dietetics
Letizia Pelusi, Domitilla Mandatori, Nadia Di Pietrantonio, Francesco Del Pizzo, Pamela Di Tomo, Natalia Di Pietro, Roberto Buda, Salvatore Genovese, Francesco Epifano, Assunta Pandolfi, Serena Fiorito, Caterina Pipino
Summary: Bone physiology is regulated by osteoblast and osteoclast activities. This study demonstrates that the coumarin derivative umbelliferon (UF) enhances the activity of osteoblasts from osteoporotic patients through estrogen receptor 1 (ESR1) and the activation of the beta-catenin pathway. UF also reduces the multinucleated cells involved in bone resorption. These findings suggest that UF may be a potential natural compound for the prevention and treatment of bone loss diseases.
Article
Biotechnology & Applied Microbiology
Caterina Pipino, Angel Bernabe-Garcia, Ilaria Cappellacci, Javier Stelling-Ferez, Pamela Di Tomo, Manuela Santalucia, Carlos Navalon, Assunta Pandolfi, Francisco Jose Nicolas
Summary: This study investigated the potential role of amniotic membrane (AM) on endothelial cells affected by gestational diabetes. The results showed that AM can reduce inflammation, improve vessel formation, and promote cell migration, which suggests its potential therapeutic use in diabetic foot ulcers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Ana I. Flores, Caterina Pipino, Urska Dragin Jerman, Sergio Liarte, Florelle Gindraux, Mateja Erdani Kreft, Francisco J. Nicolas, Assunta Pandolfi, Larisa Tratnjek, Bernd Giebel, Michela Pozzobon, Antonietta R. Silini, Ornella Parolini, Guenther Eissner, Ingrid Lang-Olip
Summary: Perinatal derivatives (PnD) have shown therapeutic potential in the treatment of various diseases, particularly in inflammation, angiogenesis, and wound healing. This review provides an overview of the modes of action of non-hematopoietic PnD in these processes, describing their sources, types, and effects. In addition, the in vitro assays currently used to evaluate the functional and therapeutic properties of PnD are evaluated.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Health Care Sciences & Services
Mario Luca Morieri, Caterina Pipino, Alessandro Doria
Summary: Pharmacogenetics, a branch of precision medicine, has the potential to be a new tool in reducing the burden of cardiovascular disease in diabetes. Improvements in cardiovascular risk stratification have been achieved by incorporating genetic characteristics into clinical data, from modest results with genetic risk scores to better performances with polygenic risk scores based on millions of variants. The use of polygenic risk scores has also shown promising results in identifying different responses to cardio-preventive treatment.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Nutrition & Dietetics
Konstantinos Efthymakis, Giuseppina Bologna, Pasquale Simeone, Laura Pierdomenico, Giulia Catitti, Simone Vespa, Angelo Milano, Domenico De Bellis, Francesco Laterza, Assunta Pandolfi, Caterina Pipino, Michele Sallese, Marco Marchisio, Sebastiano Miscia, Matteo Neri, Paola Lanuti
Summary: Extracellular vesicles (EVs) play important roles in intercellular communication, homeostasis maintenance, and diseases. This study aimed to assess and characterize circulating EVs in celiac disease patients and patients on a gluten-free diet (GFD) using a novel flow cytometry technique. The results showed significantly increased total EV counts in both newly diagnosed celiac disease patients and patients on GFD, while endothelial-derived and epithelial-derived EV counts were significantly decreased in subjects on gluten exclusion. These findings indicate that EVs could serve as potential disease-specific biomarkers in celiac disease.
Article
Biochemistry & Molecular Biology
Valeria Schiavone, Tea Romasco, Nadia Di Pietrantonio, Stefania Garzoli, Carola Palmerini, Pamela Di Tomo, Caterina Pipino, Domitilla Mandatori, Rossella Fioravanti, Elena Butturini, Manuela Sabatino, Maria Pompea Antonia Baldassarre, Rino Ragno, Assunta Pandolfi, Natalia Di Pietro
Summary: Essential oils derived from aromatic plants have been found to improve the lipid and glycemic profile of patients with diabetes mellitus. This study investigated the potential anti-inflammatory effect of anise and laurel essential oils on endothelial cells isolated from females with gestational diabetes mellitus. The results showed that both essential oils significantly reduced monocyte adhesion, VCAM-1 expression, and NF-κB p65 nuclear translocation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Materials Science, Biomaterials
Nadia Di Pietrantonio, Ilaria Cappellacci, Domitilla Mandatori, Maria Pompea Antonia Baldassarre, Assunta Pandolfi, Caterina Pipino
Summary: Type 2 diabetes is a global health problem, and cardiovascular disease is a major cause of morbidity and mortality in patients with type 2 diabetes. It is crucial to prevent the onset of cardiovascular disease, and identifying the early phase of endothelial dysfunction through novel approaches may significantly reduce its occurrence. Epigenetics and metabolomics have emerged as promising tools in understanding the regulatory networks impacting early physiological processes in the endothelium and developing disease biomarkers and targeted therapies.
Meeting Abstract
Endocrinology & Metabolism
C. Pipino, I. Cappellacci, P. Di Tomo, A. Bernabe-Garcia, J. Stelling-Ferez, N. Di Pietrantonio, L. Pelusi, M. P. A. Baldassarre, F. J. Nicolas, A. Pandolfi
Meeting Abstract
Cell & Tissue Engineering
Francisco J. Nicolas, Caterina Pipino, Angel Bernabe-Garcia, Pamela Di Tomo, Javier Stelling-Ferez, Ilaria Cappellacci, Silvia Pappada, Domitilla Mandatori, Letizia Pelusi, Assunta Pandolfi
TISSUE ENGINEERING PART A
(2022)
