Article
Multidisciplinary Sciences
Hui Ding, Kai-yun Wang, Si-yang Chen, Kai-Wen Guo, Wen-hong Qiu
Summary: This study explored the role of PTGIS in colorectal cancer through comprehensive bioinformatics analysis and in vitro experiments. The expression of PTGIS gene in colorectal cancer tissue was significantly lower than that in normal colorectal tissue, and high expression of PTGIS gene was associated with poor prognosis in patients. The study also found that PTGIS-related genes were mainly enriched in metabolic pathways, arachidonic acid metabolism, steroid biosynthesis, and cancer pathways. Cell experiments showed that high expression of PTGIS promoted proliferation, invasion, and migration ability of colorectal cancer cells.
SCIENTIFIC REPORTS
(2023)
Article
Nutrition & Dietetics
Ali Mahmoudi, Stephen L. Atkin, Nikita G. Nikiforov, Amirhossein Sahebkar
Summary: This study investigated the effect of curcumin on critical genes and pathways related to diabetes. The results showed that curcumin targeted several important genes involved in diabetes and may have potential as a therapeutic agent for diabetes.
Article
Oncology
Dorota Anusewicz, Magdalena Orzechowska, Andrzej K. Bednarek
Summary: The Notch signaling pathway regulates cell differentiation and is associated with carcinogenesis. Notch pathway members can be either oncogenic or suppressive depending on tissue context, showing promising potential for new treatment strategies.
Article
Oncology
Ruyun Cai, Qian Lu, Da Wang
Summary: The study identified key genes and miRNAs involved in liver metastasis of CRC through analysis of gene expression databases, revealing their roles in inflammatory microenvironment and EMT formation. Low expression of GAS1 and hsa-miR-33b-5p were associated with overall survival in CRC patients, with GAS1 also being linked to immune cell infiltration in tumors.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2021)
Article
Multidisciplinary Sciences
Fengjun Zhang, Cheng Yu, Wenchang Xu, Xiao Li, Junchen Feng, Hongshuo Shi, Jingrong Yang, Qinhua Sun, Xianyi Cao, Lin Zhang, Min Peng
Summary: This study utilized bioinformatics methods to identify critical genes related to COVID-19 Myocarditis, explore biological mechanisms and gene regulatory networks. Functional enrichment analysis revealed common genes were mainly enriched in biological processes such as cell cycle and ubiquitin-protein hydrolysis. Additionally, 6 critical genes and 10 potential target drugs were identified, providing a new perspective for the pathogenesis and treatment of COVID-19 Myocarditis.
Article
Oncology
Angelina S. Bortoletto, Ronald J. Parchem
Summary: Extensive studies have shown that misregulation of individual miRNAs is associated with cancer. Recently, it has been discovered that mutations in the miRNA biogenesis and processing machinery are also involved in several malignancies. These mutations can cause global miRNA misregulation and contribute to cancer development. Additionally, it has been found that mutant KRAS can affect the activity of miRNA regulatory pathway members, further promoting tumorigenesis. Targeting both mutant KRAS and the miRNA core machinery may present a potential strategy for cancer treatment.
Article
Medicine, General & Internal
Xin Ke, Hao Wu, Yi-Xiao Chen, Yan Guo, Shi Yao, Ming-Rui Guo, Yuan-Yuan Duan, Nai-Ning Wang, Wei Shi, Chen Wang, Shan-Shan Dong, Huafeng Kang, Zhijun Dai, Tie-Lin Yang
Summary: This study introduces a novel method called individualized pathway activity measurement (IPAM) for analyzing the dysregulation of biological pathways in cancer. IPAM accurately quantifies pathway activity and shows better performance in cancer classification and prognosis prediction compared to other tools. The study identifies characteristic dysregulated pathways in different cancer types and confirms the dominant role of metabolic pathways in cancer development. The findings provide new insights into the pathological mechanisms of cancer and have the potential to contribute to personalized medicine.
Article
Nutrition & Dietetics
Ali Mahmoudi, Alexandra E. Butler, Muhammed Majeed, Maciej Banach, Amirhossein Sahebkar
Summary: Curcumin may improve or inhibit the progression of NAFLD by modulating genes and proteins associated with the disease, specifically targeting stress and lipid metabolism pathways.
Article
Biochemistry & Molecular Biology
Ming Hu, Huanjing Bi, Deana Moffat, Margaret Blystone, Lillian DeCostanza, Tchilabalo Alayi, Kaiming Ye, Yetrib Hathout, Sha Jin
Summary: This study conducted a proteomic analysis of porcine decellularized pancreatic extracellular matrix (dpECM) from subjects of different ages and genders, revealing significant differences in protein composition. The detergent-free decellularization method was shown to preserve more proteins compared to a detergent-based method. The study provides insights into the potential variations in dpECM protein profiling based on age and gender, which can be valuable for tissue engineering applications.
Article
Genetics & Heredity
Xiaojie Liang, Zhaoxiang Cheng, Xinhao Chen, Jun Li
Summary: Our study established a prognostic signature consisting of 6 genes and a prognostic nomogram that can significantly assess the prognosis of patients with CRC. In addition, the exploration of signaling pathways regulated by the prognostic signature and immune correlation provides valuable information.
FRONTIERS IN GENETICS
(2022)
Article
Cell Biology
Rui-Qi Wang, Wei Zhao, Hai-Kui Yang, Jia-Mei Dong, Wei-Jie Lin, Fa-Zhong He, Min Cui, Zhi-Ling Zhou
Summary: The study comprehensively analyzed the consensus molecular subtypes (CMS) of colorectal cancer (CRC) patients using single-cell RNA sequencing data, revealing that CMS1 is more closely related to immune cells and that monocytes and macrophages play dominant roles in the CRC tumor microenvironment. Gene regulation networks showed that the critical transcription factor ERG is universally activated and upregulated in all CMS, performing diverse roles by regulating the expression of different downstream genes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Cheng Liu, Chun Yu, Guoxin Song, Xingchen Fan, Shuang Peng, Shiyu Zhang, Xin Zhou, Cheng Zhang, Xiangnan Geng, Tongshan Wang, Wenfang Cheng, Wei Zhu
Summary: MicroRNA (miRNA) plays a role as post-transcriptional regulators of mRNAs by base-pairing with complementary sequences within mRNAs, which are involved in the complex interaction between immune system and tumors. In this study, we investigated the expression profiles of miRNAs and target mRNAs and their associations with phenotypic hallmarks of colorectal cancers (CRC). Through integrative analysis of transcriptomic, immunophenotype, methylation, mutation, and survival data, we identified two pivotal regulatory pairs (miR-139-5p/STC1 and miR-20a-5p/FGL2) and validated the diagnostic value of a complex model combining these signatures.
Article
Environmental Sciences
Kai Dai, Chen Wang, Wu Yao, Changfu Hao
Summary: This study explored the distribution characteristics and biological function of miRNAs in subjects exposed to benzene series. The results showed that miR-181a-5p, 221-3p, 223-3p, 342-3p, and 638 exhibited dose-response relationship with benzene series and were closely related to multiple tumor pathways. These miRNAs may be involved in the carcinogenic process of benzene series and can be used to evaluate early biological effects and monitor exposure levels.
Article
Oncology
Jing Shi, Yong-Qun Zhang, Dou-Dou Hao, Su-Hong Fu, Jin-Li Meng
Summary: This study aimed to identify hub genes and pathways in the process of islet culture by using bioinformatic analysis. The results revealed overexpressed and underexpressed genes in cultured tissue, with KEGG pathway enrichment analyses pointing towards TGF-beta, MAPK, and VEGF signaling pathways. Specific genes like FN1, MKI67, IGF1, MAPK14, and COL1A1 may play a role in islet culture and survival. Overall, this work provides insights into the regulation and mediation of islet survival.
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Qi Wang, Xufeng Huang, Shujing Zhou, Yuntao Ding, Huizhi Wang, Weiye Jiang, Min Xu
Summary: We identified 704 differentially expressed genes (DEGs) by intersecting three datasets and further analyzed them using various gene analysis databases and visualization tools. We obtained 15 seed genes and found statistically significant survival differences in several genes in CRC. Through further research and data compilation, we emphasized the potential of IL1RN and PRRX1 as markers of immune infiltrates in CRC.
INTERNATIONAL JOURNAL OF GENOMICS
(2022)
Article
Biochemistry & Molecular Biology
Hongyan Lai, Yuchen Li, Hena Zhang, Jia Hu, Jiatao Liao, Ying Su, Qin Li, Bing Chen, Caiping Li, Zhen Wang, Yan Li, Jialei Wang, Zhiqiang Meng, Zhaohui Huang, Shenglin Huang
Summary: Extracellular vesicles contain diverse RNA species with specialized functions, and exoRBase 2.0 is an updated database that stores EV long RNAs derived from various human body fluids. This database not only integrates and compares exLR expression profiles, but also visualizes functional changes and origins of circulating EVs in different physiological and pathological conditions.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Yuan Yin, Bingxin Liu, Yulin Cao, Surui Yao, Yuhang Liu, Guoying Jin, Yan Qin, Ying Chen, Kaisa Cui, Leyuan Zhou, Zehua Bian, Bojian Fei, Shenglin Huang, Zhaohui Huang
Summary: This study reveals that colorectal cancer cells can induce macrophage polarization and PD-L1 expression through the release of small extracellular vesicles (sEVs), leading to suppressed CD8(+) T cell activity and increased tumor growth. The miR-21-5p and miR-200a derived from sEVs play key regulatory roles in this process. Inhibiting the secretion of specific sEV-miRNAs from CRC and targeting PD-L1 in TAMs may serve as novel methods for CRC treatment.
Article
Cell Biology
Fengyan Han, Beibei Yang, Mingyue Zhou, Qiong Huang, Minglang Mai, Zhaohui Huang, Maode Lai, Enping Xu, Honghe Zhang
Summary: This study reveals the co-regulatory mechanism of alternative splicing and transcription elongation in tumors. The researchers found that GLTSCR1 decreases the transcription elongation rate of the ZO1 gene, promoting the binding of a specific splicing factor and the splicing of an exon, which suppresses the migration and invasion of colorectal cancer cells.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2022)
Article
Oncology
Shujuan Ni, Xin Wang, Jinjia Chang, Hui Sun, Weiwei Weng, Xu Wang, Cong Tan, Meng Zhang, Lei Wang, Zhaohui Huang, Dan Huang, Midie Xu, Weiqi Sheng
Summary: This study evaluated the HER2 status in a large cohort of Chinese colorectal cancer patients and found a positive correlation between HER2 expression and female gender. However, there was no significant correlation between HER2 status and patient survival according to any evaluation criterion used. Furthermore, HER2 protein expression was significantly negatively correlated with RAS/BRAF mutations according to the HERACLES criteria.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Haofei Hong, Han Lin, Dan Li, Liang Gong, Kun Zhou, Yanchun Li, Hangyan Yu, Kai Zhao, Jie Shi, Zhifang Zhou, Zhaohui Huang, Zhimeng Wu
Summary: In this study, we developed a dual-targeting non-IgG format of bispecific antibodies (BsAbs) that can simultaneously target EGFR and HER2 on tumor cells. The BsAbs retained dual-targeting activity and exerted potent anticancer effects through the engagement of endogenous antibodies. The optimized BsAbs also showed improved pharmacokinetics and efficient inhibitory effects against tumor growth. This research provides a general and cost-effective platform for generating new BsAbs for cancer immunotherapy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Oncology
Kaisa Cui, Surui Yao, Bingxin Liu, Shengbai Sun, Liang Gong, Qilin Li, Bojian Fei, Zhaohui Huang
Summary: In this study, a new TME-based molecular subtype was developed using only two genes (CTSL and ZBTB7B) to identify a high-risk subgroup with unique tumor microenvironment features. This finding has potential implications for selecting treatment and management strategies for gastric cancer.
BRITISH JOURNAL OF CANCER
(2022)
Review
Oncology
Fan Yang, Zehua Bian, Peiwen Xu, Shengbai Sun, Zhaohui Huang
Summary: MiR-204-5p is a non-coding RNA molecule that regulates gene expression and is frequently downregulated in various cancer types. It has been found to suppress tumor proliferation, metastasis, autophagy, and chemoresistance, making it a potential prognostic biomarker and therapeutic target for cancer.
Article
Biochemistry & Molecular Biology
Tengyu Wang, Qinghao Zhen, Tong Wu, Lan Jin, Surui Yao, Yuyang Feng, Jinghua Chen, Chen Chen, Zhaohui Huang
Summary: GABRP is identified as a potential drug target for mediating oxaliplatin or 5-FU resistance in colorectal cancer.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Pathology
Fan Yang, Peiwen Xu, Surui Yao, Min Li, Zehua Bian, Zhaohui Huang
Summary: This study found that the signaling receptor and transporter STRA6 is highly up-regulated in colorectal cancer (CRC) and promotes CRC cell proliferation. It was also discovered that STRA6 suppresses apoptosis by controlling BCL2 expression, leading to resistance to LOHP treatment in CRC. These findings demonstrate that STRA6 is a potential prognostic factor and oncogene in CRC by promoting tumor growth and chemoresistance.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Respiratory System
Liuying Huang, Yuan Yin, Danqi Qian, Yulin Cao, Duo Wang, Xiaohan Wu, Liang Ming, Zhaohui Huang, Leyuan Zhou
Summary: Lung cancer, especially non-small cell lung cancer (NSCLC), has high morbidity and mortality rates worldwide. Severe pulmonary hemorrhage (PH) is a potential adverse event in the treatment of lung cancer with bevacizumab. This study aimed to investigate the underlying causes for the observed clinical differences between patients with lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) after bevacizumab treatment.
JOURNAL OF THORACIC DISEASE
(2023)
Editorial Material
Biochemistry & Molecular Biology
Xue Wang, Han Cheng, Die Hu, Ying Chen, Waseem Hassan, Jing Zhao, Jiuming Li, Zhaohui Huang
Article
Clinical Neurology
Yan-Yan Chen, Zhi-Cheng Gong, Mei-Mei Zhang, Zhao-Hui Huang
Summary: In this study, emodin was encapsulated in a PEG/cRGD-modified liposome to achieve brain-targeted delivery and maximize therapeutic efficacy. The study revealed that encapsulated emodin significantly enhanced its accumulation in the infarct region and improved its therapeutic efficacy. Furthermore, the critical target of emodin responsible for alleviating ischemic stroke was identified, providing a potential drug delivery strategy for ischemic stroke and other brain injuries.
TRANSLATIONAL STROKE RESEARCH
(2023)
Article
Oncology
Lu Tian, Liang Gong, Chu Hao, Yuyang Feng, Surui Yao, Bojian Fei, Xue Wang, Zhaohui Huang
Summary: In this study, we found that ELOA is upregulated in gastric cancer and is associated with malignant characteristics and poor prognosis. Functional experiments showed that ELOA promotes gastric cancer proliferation and metastasis. Mechanistic studies revealed that ELOA exerts its oncogenic properties by activating the expression of RBP1.
Article
Biochemistry & Molecular Biology
Peng Teng, Kaisa Cui, Surui Yao, Bojian Fei, Feng Ling, Chaoqun Li, Zhaohui Huang
Summary: In this study, researchers found that ME2 is highly expressed in human colorectal cancer (CRC) tissues and its knockdown inhibits CRC cell proliferation. They also discovered that SIRT5 can desuccinylate ME2, activating its enzymatic activity. Activated ME2 enhances mitochondrial respiration, supporting cell proliferation and tumor development. Additionally, the levels of succinylated ME2 and SIRT5 are associated with patient prognosis.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Cell Biology
Jia Zhang, Kaisa Cui, Liuying Huang, Fan Yang, Shengbai Sun, Zehua Bian, Xue Wang, Chaoqun Li, Yuan Yin, Shengling Huang, Leyuan Zhou, Bojian Fei, Zhaohui Huang
Summary: SLCO4A1-AS1 is upregulated in colorectal cancer (CRC) and associated with poor prognosis. SLCO4A1-AS1 acts as a molecular scaffold to regulate the Hsp90/Cdk2/c-Myc axis and promote the growth of CRC.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Genetics & Heredity
Xiangge Meng, Chengping Li, Yu Hei, Xiang Zhou, Guoli Zhou
Summary: The study used IVT-SAPAS sequencing to analyze the dynamic changes of APA sites during adipogenesis in bovine subcutaneous preadipocytes and intramuscular preadipocytes. The results showed that APA plays an important role in adipocyte differentiation, and UTR-APA switching genes have different trends, with intramuscular preadipocytes tending to use shorter 3'UTR for differentiation. TRIB3, WWTR1, and INSIG1 play important roles in intramuscular preadipocyte differentiation.
Article
Genetics & Heredity
S. Alizadeh, S. Khamse, N. Tajeddin, H. R. Khorram Khorshid, A. Delbari, M. Ohadi
Summary: This study identifies a specific genotype at a CG-rich trinucleotide short tandem repeat (STR) locus that is associated with late-onset neurocognitive disorders such as Alzheimer's disease (AD).
Article
Genetics & Heredity
Shougang Liu, Zhe Zhuang, Fanghua Liu, Xiuqing Yuan, Zeqiao Zhang, Xiaoqian Liang, Xinhui Li, Yongfeng Chen
Summary: This study investigated the effect and mechanism of RPL9 and TIFA in scalp psoriasis, and identified RPL9 as a potential therapeutic target for scalp psoriasis.
Article
Genetics & Heredity
Shiting Wang, Jiaqi Chen, Zhichao Jin, Ying Xing, Ruiping Wang
Summary: This study suggests a causal association between hair color and skin cancers, with light hair colors (red, blonde, and light brown) being associated with an increased risk and dark brown hair being associated with a decreased risk.
Article
Genetics & Heredity
Joseph Hawadak, Loick Pradel Kojom Foko, Rodrigue Roman Dongang Nana, Karmveer Yadav, Veena Pande, Aparup Das, Vineeta Singh
Summary: This study investigates the genetic diversity and natural selection of the Pfama-1 gene in Plasmodium falciparum isolates from Cameroon. The study finds a considerable nucleotide and haplotype diversity, as well as specific mutations in Cameroonian isolates. Positive diversifying selection and the identification of selected codon sites suggest the potential implication of these genetic variations in host immune pressure and parasite-binding complex modulation. The findings provide valuable baseline data for malaria vaccine design.
Article
Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
Article
Genetics & Heredity
Yunxiao Wei, Yuhan Song, Muhammad Aamir Khan, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Rui Zhang
Summary: This study analyzed the GhTPP protein family in upland cotton for the first time and identified the important role of GhTPPA_2 in regulating sugar metabolism, improving soluble sugar accumulation, and drought stress tolerance.
Article
Genetics & Heredity
Yannan Geng, Rui Shao, Tiantong Xu, Lilong Zhang
Summary: A novel risk model based on SCUBE3, TNNC1, SPON1, SEPT12 and ULBP1 genes was developed for predicting PMOP risk, with higher risk score indicating higher risk of suffering from PMOP. Significant differences in signaling pathway activities were observed between the high-risk score group and the low-risk score group.
Article
Genetics & Heredity
Randy P. Williams, Corina Lesseur, Haoxiang Cheng, Qian Li, Maya Deyssenroth, Christopher D. Molteno, Ernesta M. Meintjes, Sandra W. Jacobson, Joseph L. Jacobson, Helen Wainwright, Ke Hao, Jia Chen, R. Colin Carter
Summary: The study suggests that heavy alcohol exposure during pregnancy may impact the proportion of fetal placental villi macrophages and increase the expression of inflammatory genes. Further research is needed to explore these effects and evaluate the potential functional roles of placental inflammation in FASD.
Article
Genetics & Heredity
Lvjing Luo, Lishuang Sun, Shu Li, Huiting Liu, Zhengyu Chen, Shi Huang, Yinyin Mo, Genliang Li
Summary: This study analyzed the expression of Ptpn1 and miR-124-3p in testicular tissues of mice and investigated their regulatory relationship. The results showed that Ptpn1 expression was up-regulated in adult mouse testis compared to juvenile mouse testis, while miR-124-3p expression showed an opposite pattern. Further analysis suggested that the down-regulation of miR-124-3p may contribute to the high expression of Ptpn1 in adult mouse testis.
Article
Genetics & Heredity
Gairui Li, Dan Zhao, Xiaolin Peng, Yashuang Zhao
Summary: MiRNA-22 shows potential as a candidate for early diagnosis of colorectal cancer (CRC). The study found higher miR-22 expression levels in the CRC and CRA groups, suggesting its potential as a biomarker for CRC early screening.
Article
Genetics & Heredity
Zhongqiu Zhu, Qianting Yang, Xiaoying Tian, Da Man, Jian Wang, Junfang Zhang, Bingshe Han
Summary: This study constructed a ceRNA network mediated by lncRNAs in cold-acclimated zebrafish ZF4 cells and revealed that upregulation of MSTRG3207 promotes apoptosis by sponging dre-miR-736 during cold acclimation.
Article
Genetics & Heredity
Weitao Chen, Denggao Xiang, Shang Gao, Shuli Zhu, Zhi Wu, Yuefei Li, Jie Li
Summary: Dam construction has negatively impacted the genetic diversity and structure of fish populations. This case study on the endangered Hemibagrus guttatus found low genetic diversity, high levels of inbreeding, and decreasing population size in fragmented populations. Genetic structure and differentiation were also observed, indicating the influence of dams on these fish populations.
Article
Genetics & Heredity
Pooja Singh, Debleena Guin, Bijay Pattnaik, Ritushree Kukreti
Summary: Through systematic literature review and meta-analysis, it was found that idiopathic pulmonary fibrosis is significantly associated with 222 polymorphisms in 118 genes. Four polymorphisms - rs35705950/MUC5B, rs2736100/TERT, rs2076295/DSP, and rs111521887/TOLLIP, exhibited substantial epidemiological evidence supporting their association with IPF risk.
Article
Genetics & Heredity
Jianping Zhang, Zhijun Cai, Fanzhe Feng, Yufeng Peng, Yi Cui, Yongiqing Xu
Summary: This study found that exosomes secreted by young BMSCs can promote the healing of tendon-bone interface after rotator cuff tears. These exosomes can improve extracellular matrix remodeling, osteogenic differentiation, angiogenesis, and stemness of tendon-derived stem cells (TDSCs). The exosomes from young BMSCs have better effects compared to those from aged BMSCs.