Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Review
Chemistry, Multidisciplinary
Zhuolun Hao, Wenli Qi, Jiaming Sun, Muran Zhou, Nengqiang Guo
Summary: Although there are methods for treating wounds clinically, chronic wounds still pose challenges due to excessive inflammation, difficulties in epithelialization, vascularization, and other factors. Recent research on adipose-derived stem cells (ADSCs) has shown that they can promote the healing of chronic wounds by regulating macrophage function, cellular immunity, and promoting angiogenesis and epithelialization. This study reviews the difficulties in treating chronic wounds and the advantages and mechanisms of ADSCs in promoting their healing to provide a reference for stem cell therapy.
FRONTIERS IN CHEMISTRY
(2023)
Review
Cell & Tissue Engineering
Nicolo C. Brembilla, Hubert Vuagnat, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: Chronic wounds have a significant impact on quality of life and healthcare costs. Adipose-derived stromal cells (ASC) have emerged as a promising therapy for chronic wounds. Studies have shown the clinical efficacy of ASC-based therapies in treating chronic wounds.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Jiateng Hu, Yihong Jiang, Xiaoyu Wu, Zhaoyu Wu, Jinbao Qin, Zhen Zhao, Bo Li, Zhijue Xu, Xinwu Lu, Xin Wang, Xiaobing Liu
Summary: ADSC-exos miR-17-5p regulates AAA progression and inflammation via the TXNIP-NLRP3 signaling pathway, providing a novel insight in AAA treatment.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Bin Wang, Li Li, Ruizhu Yu
Summary: Chronic endometritis (CE) is strongly associated with reproductive failure. Exosome-based therapy shows promise in inflammation-related disorders, but its use in CE therapy is limited. In this study, an in vitro CE model was established using lipopolysaccharide (LPS) in human endometrial stromal cells (HESCs). Exosomes from adipose tissue-derived stem cells (ADSCs) were found to enhance cell proliferation and suppress apoptosis in LPS-treated HESCs. Exosomes also decreased the levels of inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta. Furthermore, Exos exposure reduced inflammation in a mouse model of CE. Mechanistically, the anti-inflammatory effects of Exos were mediated through the miR-21/TLR4/NF-kB signaling pathway. These findings suggest that ADSC-Exo-based therapy could be a promising strategy for treating CE.
CELL TRANSPLANTATION
(2023)
Article
Biochemistry & Molecular Biology
Nicolo Costantino Brembilla, Ali Modarressi, Dominik Andre-Levigne, Estelle Brioudes, Florian Lanza, Hubert Vuagnat, Stephane Durual, Laurine Marger, Wolf-Henning Boehncke, Karl-Heinz Krause, Olivier Preynat-Seauve
Summary: This study evaluates the influence of a gelatin sponge on adipose-derived stromal cells (ASC). The results show that the gelatin sponge strongly influences the transcriptome of ASC and acted as a concentrator and reservoir of the regenerative ASC secretome. The gelatin sponge also increased angiogenesis in vivo and promoted wound healing.
Article
Biotechnology & Applied Microbiology
Gen Li, Yin Zhang, Jiezhou Wu, Renhao Yang, Qi Sun, Yidong Xu, Bo Wang, Ming Cai, Yang Xu, Chengyu Zhuang, Lei Wang
Summary: This study explores the effects and mechanism of adipose stem cells-derived exosomes (ADSCs-Exos) and ADSCs-Exos modified tissue engineering scaffold on bone defect repair. The results demonstrate that ADSCs-Exos promote the proliferation, migration, and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and ADSCs-Exos modified scaffold enhances the repair of BMSCs and bone defects both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biophysics
Liyang Wang, Wei Cheng, Jingjing Zhu, Wenyao Li, Danyang Li, Xi Yang, Weixin Zhao, Mingjun Ren, Jieji Ren, Xiumei Mo, Qiang Fu, Kaile Zhang
Summary: Regeneration of urethral defects has been a challenge in clinical practice. A nanoyarn scaffold delivering adipose-derived stem cells' exosomes was fabricated to address this issue. The scaffold showed good biocompatibility and promoted wound healing in experiments.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Pharmacology & Pharmacy
Hsiang-Hao Hsu, Aline Yen Ling Wang, Charles Yuen Yung Loh, Ashwin Alke Pai, Huang-Kai Kao
Summary: This study found that diabetic ASC-Exo enhance cutaneous wound healing by stimulating dermal cell proliferation, keratinocyte proliferation, and angiogenesis. The diabetic ASC-Exo also stimulate resident monocytes/macrophages to secrete more TGF-beta 1 and activate the TGF-beta/Smad3 signaling pathway.
Article
Cell Biology
Zhongfei Zhang, Xiaoxiong Zou, Run Zhang, Yu Xie, Zhiming Feng, Feng Li, Jianbang Han, Haitao Sun, Qian Ouyang, Shiting Hua, Bingke Lv, Tian Hua, Zhizheng Liu, Yingqian Cai, Yuxi Zou, Yanping Tang, Xiaodan Jiang
Summary: The study showed that hUMSC-Exos can attenuate microglia-mediated neuroinflammation and improve neural deficits following ischemic stroke. MiR-146a-5p plays a crucial role in this process.
Article
Biotechnology & Applied Microbiology
Ziwan Ji, Zhongming Cai, Shuming Gu, Yucang He, Zikai Zhang, Tian Li, Qing Wei, Jingping Wang, Chen Ke, Liqun Li
Summary: The study found that adipose-derived exosomes (ADEs) can inhibit adipogenic differentiation of adipose-derived stem cells by activating the Hedgehog (Hh) signaling pathway, thereby reducing the adverse effects of obesity on wound healing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Cell Biology
Mehrdad Hajinejad, Sajad Sahab-Negah
Summary: Neuroinflammation plays a crucial role in neurological disorders, and mesenchymal stem cells can modulate inflammation and microglia/macrophage polarization through exosome secretion. Studies have shown that miRNAs contained in exosomes have the potential to impact recipient cell behavior, providing a novel therapeutic approach for addressing inflammatory pathways in neurological diseases.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Xing Zhang, Yihong Jiang, Qun Huang, Zhaoyu Wu, Hongji Pu, Zhijue Xu, Bo Li, Xinwu Lu, Xinrui Yang, Jinbao Qin, Zhiyou Peng
Summary: Exosomes secreted by ADSCs overexpressing GLO-1 promoted endothelial cell protection and angiogenesis in diabetic mice with limb ischemia, showing promising potential for clinical treatment.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Yang An, Shuyan Lin, Xiaojie Tan, Shiou Zhu, Fangfei Nie, Yonghuan Zhen, Luosha Gu, Chunlei Zhang, Baicheng Wang, Wei Wei, Dong Li, Junhao Wu
Summary: ADSCs-EXOs modulate immune responses and inflammation, promote angiogenesis, accelerate proliferation and re-epithelization of skin cells, and regulate collagen remodelling to inhibit scar hyperplasia in wounds. Compared with ADSCs therapeutics, ADSCs-EXOs have high stability, easy storage, immune system non-rejection, homing effect, and easily controlled dosage. In patients with diabetes mellitus, ADSCs-EXOs can improve fat grafting and promote wound healing.
CELL PROLIFERATION
(2021)
Article
Cell & Tissue Engineering
Yang Zhou, Xing-Liao Zhang, Shou-Tao Lu, Ning-Yan Zhang, Hai-Jun Zhang, Jing Zhang, Jun Zhang
Summary: This study explores the potential clinical application of hADSCs-Exos encapsulated in PF-127 hydrogel for wound healing. The results showed that PF-127/hADSCs-Exos complexes enhanced skin wound healing, promoted re-epithelialization, increased collagen synthesis and expression of skin barrier proteins, and reduced inflammation. Using PF-127/hADSCs-Exos complexes allows for lower doses and frequency of exosome administration while maintaining therapeutic effects.
STEM CELL RESEARCH & THERAPY
(2022)