Article
Biology
Vincent Grass, Emilie Hardy, Kassian Kobert, Soheil Rastgou Talemi, Elodie Decembre, Coralie Guy, Peter Markov, Alain Kohl, Mathilde Paris, Anja Bockmann, Sara Munoz-Gonzalez, Lee Sherry, Thomas Hofer, Bastien Boussau, Marlene Dreux
Summary: This study investigates the genetic evolution of Zika virus (ZIKV) in a controlled host cell environment. The results show that serially passaged ZIKV acquired increased infectivity and developed resistance to TLR3-induced antiviral response. This adaptation is cell-type specific and is likely due to a reduced time-lag between infection and viral replication. Deep-sequencing analysis identified mutations that temporally coincided with the acquisition of the adapted phenotype. Functional validation of a specific mutation in ZIKV envelope (E) protein confirmed its role in the adapted phenotype. Overall, this work provides insights into how ZIKV adapts to host cells and evades immune responses.
COMMUNICATIONS BIOLOGY
(2022)
Article
Engineering, Environmental
Kevin Zhu, Cailee Hill, Aaron Muirhead, Mausumi Basu, Joe Brown, Margo A. Brinton, Matthew J. Hayat, Cristina Venegas-Vargas, Mitermayer G. Reis, Arnau Casanovas-Massana, J. Scott Meschke, Albert I. Ko, Federico Costa, Christine E. Stauber
Summary: During the Zika virus epidemic, the capacity for widespread diagnostic testing was hindered due to serological cross-reactivity and high costs. Wastewater monitoring approaches, however, may offer a means of community-level surveillance. The persistence and recovery of Zika virus RNA were studied in experiments using different water sources, providing valuable information for future wastewater monitoring efforts.
Article
Cell Biology
Karolin I. Wagner, Laura M. Mateyka, Sebastian Jarosch, Vincent Grass, Simone Weber, Kilian Schober, Monika Hammel, Teresa Burrell, Behnam Kalali, Holger Poppert, Henriette Beyer, Sophia Schambeck, Stefan Holdenrieder, Andrea Strotges-Achatz, Verena Haselmann, Michael Neumaier, Johanna Erber, Alina Priller, Sarah Yazici, Hedwig Roggendorf, Marcus Odendahl, Torsten Tonn, Andrea Dick, Klaus Witter, Hrvoje Mijocevic, Ulrike Protzer, Percy A. Knolle, Andreas Pichlmair, Claudia S. Crowell, Markus Gerhard, Elvira D'Ippolito, Dirk H. Busch
Summary: This study isolated TCR repertoires specific for SARS-CoV-2 epitopes restricted to common HLA class I molecules in convalescent individuals. SARS-CoV-2-specific CD8(+) T cells were found to be detectable up to 12 months after infection. The TCR repertoires were diverse, with heterogeneous functional avidity and cytotoxicity towards virus-infected cells.
Article
Critical Care Medicine
Erika Wong, Fengyun Xu, Jeremie Joffre, Nina Nguyen, Kevin Wilhelmsen, Judith Hellman
Summary: ERK1/2 plays a complex role in LPS-induced inflammatory activation and permeability of human lung microvascular endothelial cells, with inhibition of ERK1/2 enhancing IL-6 and VCAM-1 production while promoting IL-6 production in monocytes. ERK1/2 activation limits IL-6 production by HMVEC but promotes HMVEC permeability, suggesting its important role in regulating endothelial cell inflammation and vascular permeability in sepsis and injury.
Article
Food Science & Technology
Hyeon-Ju Lee, Se-Young Oh, Inho Jo
Summary: This study found that Zearalenone induces endothelial cell apoptosis through activation of an ERK1/2/p53/caspase 3 signaling pathway via release of Ca2+ from the endoplasmic reticulum, independent of reactive oxygen species production and estrogen receptor activation.
Article
Virology
Blake Schouest, Alba Grifoni, John Pham, Jose Mateus, John Sydney, James D. Brien, Aruna D. De Silva, Angel Balmaseda, Eva Harris, Alessandro Sette, Daniela Weiskopf
Summary: This study identified ZIKV T cell epitopes in individuals unexposed to ZIKV but living in regions endemic to DENV, confirming the impact of preexisting DENV immunity on ZIKV infection. It also found that cross-reactive T cell populations can be expanded by stimulation with ZIKV peptides, providing insights for understanding natural immunity patterns and developing diagnostic tests and vaccines.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Xiao-Ning Zhang, Kai-Di Yang, Cong Chen, Zhi-Cheng He, Qiang-Hu Wang, Hua Feng, Sheng-Qing Lv, Yan Wang, Min Mao, Qing Liu, Yao-Yao Tan, Wen-Ying Wang, Tian-Ran Li, Lin-Rong Che, Zhong-Yi Qin, Ling-Xiang Wu, Min Luo, Chun-Hua Luo, Yu-Qi Liu, Wen Yin, Chao Wang, Hai-Tao Guo, Qing-Rui Li, Bin Wang, Wei Chen, Shuang Wang, Yu Shi, Xiu-Wu Bian, Yi-Fang Ping
Summary: The study reveals the role of pericytes in potentiating DDR signaling in GBM cells, and suggests that targeting CCL5-CCR5 signaling could be an effective therapeutic strategy to improve chemotherapeutic efficacy against GBM.
Review
Immunology
Vincent Dussupt, Kayvon Modjarrad, Shelly J. Krebs
Summary: The unprecedented Zika outbreak in the Americas from 2015-2016 led to global concern and accelerated the deployment of vaccine and therapeutic countermeasures. Several potent neutralizing antibodies against Zika and related flaviviruses have been discovered in recent years. Structure-based approaches have helped identify species-specific and cross-protective epitopes with therapeutic value.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Maria Giovanna Francipane, Bruno Douradinha, Cinzia Maria Chinnici, Giovanna Russelli, Pier Giulio Conaldi, Gioacchin Iannolo
Summary: Glioblastoma (GBM) is an aggressive neurological tumor with no effective long-term treatment options. The discovery of Zika virus (ZIKV) having oncolytic effects against GBM has led to hopes for new therapeutic approaches, but further research on its mechanisms of action is necessary for potential clinical trials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Camilo J. Acosta, Clemente Diaz, Francesco Nordio, Htay-Htay Han, Kelley J. Moss, Kelly Bohning, Pradeep Kumar, Mengya Liu, Hetal Patel, Filippo Pacciarini, Vincent Mwangi, Elke Walter, Tim D. Powell, Hana M. El Sahly, Whitney R. Baldwin, Joseph Santangelo, Evan J. Anderson, Gary Dubin
Summary: This study reports the 2-year persistence of immune response to Takeda's Zika virus vaccine (TAK-426) and compares it with natural infection. The results show that the vaccine has a long-lasting immune response and is comparable to natural infection.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Fei Xiao, Hongyu Li, Zhongxue Feng, Luping Huang, Lingmiao Kong, Min Li, Denian Wang, Fei Liu, Zhijun Zhu, Yong'gang Wei, Wei Zhang
Summary: The study found that IMD maintains an important homeostatic state by activating the ERK1/2-EGR1 signaling cascade, promoting the invasive ability of HCC cells and inducing HCC cell death through an apoptotic pathway. Blocking IMD activity may have therapeutic potential in the treatment of HCC.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Romain Marlin, Delphine Desjardins, Vanessa Contreras, Guillaume Lingas, Caroline Solas, Pierre Roques, Thibaut Naninck, Quentin Pascal, Sylvie Behillil, Pauline Maisonnasse, Julien Lemaitre, Nidhal Kahlaoui, Benoit Delache, Andres Pizzorno, Antoine Nougairede, Camille Ludot, Olivier Terrier, Nathalie Dereuddre-Bosquet, Francis Relouzat, Catherine Chapon, Raphael Ho Tsong Fang, Sylvie van der Werf, Manuel Rosa Calatrava, Denis Malvy, Xavier de Lamballerie, Jeremie Guedj, Roger Le Grand
Summary: Repurposed antiviral drugs are valuable resources for outbreak defense, but rigorous evaluation in large animal models is crucial for successful clinical implementation. This study evaluates the antiviral activity of favipiravir against Zika virus and SARS-CoV-2 in cynomolgus macaques, highlighting its potential for clinical investigations against Zika virus but advising against its use for SARS-CoV-2 infection.
NATURE COMMUNICATIONS
(2022)
Article
Pharmacology & Pharmacy
Durbadal Ojha, Rahul Basu, Karin E. Peterson
Summary: The Zika virus is an arbovirus that causes placental insufficiency, microcephaly, and other neurological complications. Currently, there are no approved therapeutics to treat Zika virus infection. In this study, researchers examined how inhibiting cellular organelles or trafficking processes affected virus replication. They found that inducing endoplasmic reticulum stress could effectively inhibit virus production, suggesting it as a potential cellular target for antiviral development.
ANTIVIRAL RESEARCH
(2023)
Article
Immunology
Julia Eder, Esther Zijlstra-Willems, Gerrit Koen, Neeltje A. A. Kootstra, Katja C. C. Wolthers, Teunis B. B. Geijtenbeek
Summary: Zika virus, transmitted mainly through mosquito bites and sexual contact, targets dendritic cells (DCs) and Langerhans cells (LCs) in both the skin and the genital tract. While DCs are efficiently infected by the virus, LCs do not become infected but are able to transmit the virus to other target cells. This highlights the role of DCs and LCs in the early stages of Zika virus infection and the importance of investigating transmission routes beyond mosquito control.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Hematology
Lingfeng Qin, Haifeng Zhang, Busu Li, Quan Jiang, Francesc Lopez, Wang Min, Jenny Huanjiao Zhou
Summary: The study reveals that CCM3 deletion increases VEGFR3-ERK1/2 signaling in lymphatic endothelial cells, leading to lymphatic hyperplasia and malformation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Virology
Jonas Nascimento Conde, Emiliana Mandarano da Silva, Diego Allonso, Diego Rodrigues Coelho, Iamara da Silva Andrade, Luciano Neves de Medeiros, Joice Lima Menezes, Angela Silva Barbosa, Ronaldo Mohana-Borges
JOURNAL OF VIROLOGY
(2016)
Article
Biochemistry & Molecular Biology
N. Dinuka Abeydeera, Martin Egli, Nehemiah Cox, Karen Mercier, Jonas Nascimento Conde, Pradeep S. Pallan, Daniella M. Mizurini, Malgorzata Sierant, Fatima-Ezzahra Hibti, Tom Hassell, Tianzhi Wang, Feng-Wu Liu, Hong-Min Liu, Carlos Martinez, Anil K. Sood, Terry P. Lybrand, Chiraz Frydman, Robson Q. Monteiro, Richard H. Gomer, Barbara Nawrot, Xianbin Yang
NUCLEIC ACIDS RESEARCH
(2016)
Review
Microbiology
Jonas N. Conde, Emiliana M. Silva, Angela S. Barbosa, Ronaldo Mohana-Borges
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Emiliana M. Silva, Jonas N. Conde, Diego Allonso, Gustavo T. Ventura, Diego R. Coelho, Pedro Henrique Carneiro, Manuela L. Silva, Marciano Paes, Kissila Rabelo, Gilberto Weissmuller, Paulo Mascarello Bisch, Ronaldo Mohana-Borges
SCIENTIFIC REPORTS
(2019)
Article
Virology
Jonas N. Conde, William R. Schutt, Megan Mladinich, Sook-Young Sohn, Patrick Hearing, Erich R. Mackow
JOURNAL OF VIROLOGY
(2020)
Article
Microbiology
Jonas Nascimento Conde, William R. Schutt, Elena E. Gorbunova, Erich R. Mackow
Article
Immunology
Cecilia B. Cavazzoni, Vicente B. T. Bozza, Tostes C. Lucas, Luciana Conde, Bruno Maia, Luka Mesin, Arien Schiepers, Jonatan Ersching, Romulo L. S. Neris, Jonas N. Conde, Diego R. Coelho, Tulio M. Lima, Renata G. F. Alvim, Leda R. Castilho, Heitor A. de Paula Neto, Ronaldo Mohana-Borges, Iranaia Assuncao-Miranda, Alberto Nobrega, Gabriel D. Victora, Andre M. Vale
Summary: The study found that Zika virus NS1 is an immunodominant viral antigen associated with the presence of autoreactive antibodies in humoral immune response. This suggests a potential role for NS1 in ZIKV-associated autoimmune manifestations.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Virology
Elena E. Gorbunova, Erich R. Mackow
Summary: The Andes virus (ANDV) activates RhoA in pulmonary microvascular endothelial cells, leading to pulmonary edema. ANDV N protein binds to RhoGDI and releases RhoA, increasing endothelial cell permeability. These findings provide a novel mechanism for targeting and preventing HPS.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Sook-Young Sohn, Janet Hearing, JoAnn Mugavero, Varvara Kirillov, Elena Gorbunova, Luke Helminiak, Smruti Mishra, Erich Mackow, Patrick Hearing, Nancy C. Reich, Hwan Keun Kim
Summary: IFN-lambda shows potential for inhibiting SARS-CoV-2 infection and reducing pathology in a mouse model. Male mice are more susceptible to severe symptoms and mortality from SARS-CoV-2 infection.
Article
Virology
Jonas N. Conde, Santiago Sanchez-Vicente, Nicholas Saladino, Elena E. Gorbunova, William R. Schutt, Megan C. Mladinich, Grace E. Himmler, Jorge Benach, Hwan Keun Kim, Erich R. Mackow
Summary: Powassan viruses (POWVs) are neurovirulent tick-borne flaviviruses emerging in the northeastern United States, with a high prevalence in Long Island (LI) deer ticks. The viruses can quickly enter the central nervous system (CNS) to cause encephalitis, with potentially fatal consequences. Recent studies have shown that POWVs can infect brain microvascular endothelial cells (hBMECs) and pericytes, potentially providing a mechanism for the virus to spread to neuronal compartments.
JOURNAL OF VIROLOGY
(2022)