Journal
WATER RESEARCH
Volume 197, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.watres.2021.117043
Keywords
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Funding
- Houston Health Department
- National Science Foundation [CBET 2029025, CBET 1932000]
- Rice University
- Environmental Research & Education Foundation scholarship
- National Academies of Science, Engineering, and Medicine Gulf Research Early Career Research Fellowship
- Johnson & Johnson WiSTEM2D award
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As the COVID-19 pandemic continues to impact communities worldwide, wastewater monitoring of the SARS-CoV-2 virus has emerged as a vital tool for predicting outbreaks. This study evaluated five different concentration methods for quantifying SARS-CoV-2 virus RNA in wastewater samples, with HA filtration and bead beating showing the best performance in terms of sensitivity and cost, providing guidance for labs conducting wastewater monitoring of SARS-CoV-2.
As the COVID-19 pandemic continues to affect communities across the globe, the need to contain the spread of the outbreaks is of paramount importance. Wastewater monitoring of the SARS-CoV-2 virus, the causative agent responsible for COVID-19, has emerged as a promising tool for health officials to an-ticipate outbreaks. As interest in wastewater monitoring continues to grow and municipalities begin to implement this approach, there is a need to further identify and evaluate methods used to concentrate SARS-CoV-2 virus RNA from wastewater samples. Here we evaluate the recovery, cost, and throughput of five different concentration methods for quantifying SARS-CoV-2 virus RNA in wastewater samples. We tested the five methods on six different wastewater samples. We also evaluated the use of a bovine coronavirus vaccine as a process control and pepper mild mottle virus as a normalization factor. Of the five methods we tested head-to-head, we found that HA filtration with bead beating performed the best in terms of sensitivity and cost. This evaluation can serve as a guide for laboratories establishing a protocol to perform wastewater monitoring of SARS-CoV-2. (C) 2021 Elsevier Ltd. All rights reserved.
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