Journal
SMALL
Volume 17, Issue 36, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.202101924
Keywords
imaging; matrix metalloproteinase-2; near-infrared; ratiometric fluorescence; semiconducting polymer nanoparticles
Categories
Funding
- National Key R&D Program of China [2017YFA0701301]
- National Natural Science Foundation of China [21922406, 21775071, 21632008]
- Natural Science Foundation of Jiangsu Province [BK20190055, BK20202004]
- Fundamental Research Funds for the Central Universities [020514380251]
- Excellent Research Program of Nanjing University [ZYJH004]
Ask authors/readers for more resources
The study developed a ratiometric fluorescent probe (SPN-MMP-RGD) by integrating Cy5.5 and PCPDTBT, enabling sensitive detection of MMP-2 activity and noninvasive imaging of tumor cells.
Enzyme-activatable ratiometric near-infrared (NIR) fluorescent probes enabling noninvasive imaging of enzyme activity in vivo are promising for biomedical research; however, such probes with ratiometric fluorescence emissions both in NIR window under a single NIR light excitation are largely unexplored. Here, a quenched NIR fluorophore of Cy5.5 is integrated with NIR fluorescent poly[2,6-(4,4-bis-(2-ethylhexyl)-4H-cyclopenta[2,1-b;3,4-b ']dithiophene)-alt-4,7(2,1,3-benzothiadiazole)] (PCPDTBT)-based semiconducting polymer nanoparticles (SPNs), and an alpha(v)beta(3) integrin-targeting and matrix metalloproteinase-2 (MMP-2)-activatable ratiometric fluorescent probe (SPN-MMP-RGD) is developed. Under excitation at 660 nm, SPN-MMP-RGD shows always-on fluorescence of PCPDTBT (830 nm) and activatable fluorescence of Cy5.5 (690 nm) toward MMP-2, affording a remarkable approximate to 176-fold enhancement in fluorescence intensity ratio between 690 and 830 nm (I-690/I-830) for sensitive detection of MMP-2 activity in vitro and in tumor cells. By virtue of ratiometric fluorescence imaging independently of probe's concentration, SPN-MMP-RGD can not only accurately report on MMP-2 levels regarding different tumor sizes, but also noninvasively delineate MMP-2-positive tiny gastric tumors metastasis in vivo. The authors' study reveals the potential of SPN-MMP-RGD for ratiometric fluorescence imaging of MMP-2 activity via combining two independent NIR fluorophores, which can be amenable for the design of other enzyme-activatable ratiometric NIR fluorescent probes for reliable in vivo imaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available