Article
Chemistry, Analytical
Tinglan Zhang, Jun Zhang, Fangfang Chen, An Liu, Jinzhu Jiang, Zhiyong Yan, Xianju Liu
Summary: In this study, we analyzed the triterpenoid compounds in different fresh tissues of Pulsatilla chinensis using UHPLC-Q-TOF-MS and UHPLC-QQQ-MS techniques. A total of 132 triterpenoids were identified, including new aglycones and new ways of rhamnose linking. We established an analytical method for triterpenoid content and found significant distribution differences in types and contents of triterpenoids among different tissues. We also identified 15 differential components between aboveground and underground tissues. This study provides an efficient analysis strategy for triterpenoids in P. chinensis and important insights into the biosynthetic pathway of triterpenoid saponins.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Yi Nan, Haizhen Liang, Xu Pang, Wei Zheng, Yuhao Shi, Xiaojuan Chen, Jie Zhang, Juan Song, Baiping Ma
Summary: In this study, an integrated method of ultra-high-performance liquid chromatography-quadruple time-of-flight mass spectrometry (UHPLC-Q-TOF/MS) and ultra-high-performance liquid chromatography-charged aerosol detection (UHPLC-CAD) was used for the qualitative and quantitative analysis of Polygonatum odoratum (PO). A total of 62 compounds were identified by UHPLC-Q-TOF/MS, and 13 potential chemical markers were screened out to distinguish two commercial specifications. The absolute determination method for three compounds, polygodoraside G, polygonatumoside F, and timosaponin H1, was established and validated by UHPLC-CAD.
FRONTIERS IN CHEMISTRY
(2023)
Article
Chemistry, Applied
Jinglei Wang, Qingzhen Wei, Wuhong Wang, Haijiao Hu, Yaqin Yan, Yikui Wang, Yan Li, Yaqin Jiang, Guanzheng Wu, Tianhua Hu, Chonglai Bao
Summary: This study identified metabolites in radishes of ten varieties using UHPLC-Q-TOF-MS, and found that the metabolite composition differed among different edible types, with greater accumulation in fruit radishes, particularly flavonoids.
Article
Chemistry, Applied
Hui Wang, Lili Hu, Xiaotu Chang, Yuge Hu, Yan Zhang, Peng Zhou, Xiaojiao Cui
Summary: A method using UHPLC-MS/MS was developed for the determination of toxoflavin and fervenulin in 6 types of food, and the degradation products were identified.
Article
Chemistry, Applied
Tiancheng Ma, Junpeng Lin, Anna Gan, Yanxiu Sun, Yu Sun, Mengshi Wang, Meiqi Wan, Tingxu Yan, Ying Jia
Summary: A high-resolution mass spectrometer was used to identify the components of Hemerocallis citrina Baroni (HF) flowers, and an UHPLC-Q-TOF-MS method with DPPH pre-column derivatization was employed to screen antioxidant active compounds. A total of 132 components were identified, including flavonoids, phenylpropanoids, lipids, alkaloids, and other compounds. Five components were recommended for the quality control of HF based on their content, correlation analysis, principal component analysis, and DPPH free radical scavenging activity.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Food Science & Technology
Yan Luo, Zhenjun Zhao, Hujiang Chen, Xueli Pan, Risheng Li, Dewen Wu, Xianchun Hu, Lingling Zhang, Huawei Wu, Xinghui Li
Summary: UHPLC-Q-TOF-MS/MS was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples. The results showed that tea polysaccharide samples extracted from different pile-fermentation stages could be grouped into three distinct groups based on their composition and abundance. The pile-fermentation process significantly affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
Article
Food Science & Technology
Rajender Kumar, Dinesh Kumar
Summary: Allium is widely consumed as a spice around the world. This study compared the metabolome and antioxidant activity of different tissues of Allium cepa, A. sativum, and A. semenovii. The results showed that A. cepa and A. semenovii had higher antioxidant activity and polyphenol content compared to A. sativum. Additionally, various metabolites including polyphenols and sulfur containing compounds were identified. This study highlights the potential of A. semenovii for utilization in food and nutraceuticals.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Wenhao Liu, Huan He, Zhiqiang Li, Qiang Zhou, Bin Zhou, Zhi Feng Li, Tongfei Zhu, Yulin Feng
Summary: In this study, the chemical composition of Sabia parvi-flora was analyzed using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. A rapid and accurate method, based on the characteristic ion filtration, was utilized to identify the chemical components, resulting in the identification of 104 components, including 12 potentially novel compounds. This method provides an important basis for basic chemical research in Sabia parvi-flora.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Applied
Ming-Yang Wang, Zhi-Li Ma, Cheng-Li He, Xiao-Yan Yuan
Summary: This study aimed to evaluate the antioxidative properties of flavonoids in Jerusalem artichoke leaves and determine their contents quantitatively. The results showed that two flavonoids extracted from Jerusalem artichoke leaves exhibited stronger antioxidant effects than the positive control, BHT. Furthermore, the total contents of these flavonoids varied significantly depending on the region and harvesting time, with the highest levels found in the flowering stage leaves from Dalian, Liaoning Province, China. Overall, this research highlights the excellent antioxidant properties of Jerusalem artichoke leaves and their potential therapeutic application in protecting against diseases caused by oxidative stress.
NATURAL PRODUCT RESEARCH
(2022)
Article
Immunology
Nan Wang, Linjiao Yang, Lili Shang, Zhaojun Liang, Yanlin Wang, Min Feng, Shuting Yu, Xiaoying Li, Chong Gao, Zhenyu Li, Jing Luo
Summary: This study identified potential diagnostic biomarkers for Psoriatic Arthritis (PsA) by analyzing fecal metabolites in comparison with Rheumatoid Arthritis (RA) and healthy controls. A total of 14 common differential metabolites were defined as candidate biomarkers, with 5 fecal metabolites determined as specific biomarkers for PsA through a support vector machines (SVM) model. Abnormality in amino acid metabolism, bile acid metabolism and lipid metabolism were found to contribute to the occurrence and development of PsA.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Applied
Limin Wang, Kai Hong, Agbaka Johnpaul, Guangsen Zhu, Chenyan Lv, Changwei Ma
Summary: Through metabolomics and sensory evaluation analysis, this study identified the key bitter components of Krausen in beer fermentation, providing guidance for further research on taste quality of beer during fermentation. The study revealed that hop bitter acid derivatives were the main contributors to the bitter profile of Krausen.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Patricia A. Da Cunha, Diana Nitusca, Luisa Matos Do Canto, Rency S. Varghese, Habtom W. Ressom, Shawna Willey, Catalin Marian, Bassem R. Haddad
Summary: Breast cancer is a leading cause of cancer-related deaths in women worldwide, and there is a need for new biomarkers for early detection. This study used metabolomic profiling to identify specific metabolic markers for breast cancer and developed a highly effective diagnostic model.
Article
Cell Biology
Yan Gao, Jun-Tong Li, Xiang Li, Xun Li, Song-Wei Yang, Nai-Hong Chen, Lin Li, Lan Zhang
Summary: The study demonstrates that TSG alleviates APAP-induced hepatotoxicity in mice by eliminating hepatic glutathione depletion and cysteine adducts formation, reducing interleukin-10 expression, and lowering reactive oxygen species production. Additionally, TSG can regulate lipid metabolism, promote antioxidant enzyme production, and decrease inflammation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Chemistry, Applied
Bei Zhou, Wen Huang, Xi Feng, Qian Liu, Salam A. Ibrahim, Ying Liu
Summary: This study investigated glucosinolate variations in Chinese cabbage cultivars at different growth periods. Thirteen glucosinolates were identified and quantified, and significant differences in glucosinolate concentrations were found between Xiayangbai and Zaoshu-5. The highest concentration of glucosinolates was observed during the seed period, with aliphatic glucosinolates being predominant in seeds, seedling, and leaves of the rosette as well as during germination. The roots, however, contained a dominant aromatic glucosinolate (gluconasturtiin), and glucoerucin was only found in the roots of rosettes. Positive significant correlations were observed among gluconapin, glucobrassicanapin, glucoraphanin, glucoalyssin, and 4-hydroxyglucobrassicin. These findings provide scientific evidence for the metabolism pathways of glucosinolates in Chinese cabbage and can contribute to the development of functional foods with higher glucosinolate content.
Article
Integrative & Complementary Medicine
Yan He, Zhenkun Zhou, Weijie Li, Yanqiong Zhang, Ruoyao Shi, Tao Li, Linlin Jin, Hongliang Yao, Na Lin, Hao Wu
Summary: This study characterized the chemical constituents and metabolism pathways of Baihu-Guizhi decoction in vivo using liquid chromatography and mass spectrometry techniques. The study identified the absorbed components and metabolites of the decoction and established a quantitative method to monitor their pharmacokinetic behaviors. The findings provide valuable insights into the pharmacological effects and mechanism of Baihu-Guizhi decoction.
