Engineering of a robust Escherichia coli chassis and exploitation for large-scale production processes

Escherichia coli metabolism under short-term repetitive substrate dynamics: adaptation and trade-offs

(2020) Eleni Vasilakou et al.   Microbial Cell Factories

Pseudomonas putida KT2440 is naturally endowed to withstand industrial-scale stress conditions

(2020) Andreas Ankenbauer et al.   Microbial Biotechnology

Simulated oxygen and glucose gradients as a prerequisite for predicting industrial scale performance a priori

(2020) Maike Kuschel et al.   BIOTECHNOLOGY AND BIOENGINEERING

A novel knock out strategy to enhance recombinant protein expression in Escherichia coli

(2020) Ashish K. Sharma et al.   Microbial Cell Factories

Engineering Robust Production Microbes for Large-Scale Cultivation

(2019) Maren Wehrs et al.   TRENDS IN MICROBIOLOGY

Broadening the Scope of Enforced ATP Wasting as a Tool for Metabolic Engineering in Escherichia coli

(2019) Simon Boecker et al.   Biotechnology Journal

Scale‐down studies for the scale‐up of a recombinant Corynebacterium glutamicum fed‐batch fermentation: loss of homogeneity leads to lower levels of cadaverine production

(2019) Williams Olughu et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Lack of cations in flow cytometry buffers affect fluorescence signals by reducing membrane stability and viability of Escherichia coli strains

(2018) Kathrin Tomasek et al.   JOURNAL OF BIOTECHNOLOGY

Insight into the large-scale upstream fermentation environment using scaled-down models

(2018) Williams Olughu et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Escherichia coli HGT: Engineered for high glucose throughput even under slowly growing or resting conditions

(2017) Annette Michalowski et al.   METABOLIC ENGINEERING

Homogenizing bacterial cell factories: Analysis and engineering of phenotypic heterogeneity

(2017) Dennis Binder et al.   METABOLIC ENGINEERING

Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads

(2017) Ryan R. Wick et al.   PLoS Computational Biology

Repetitive Short-Term Stimuli Imposed in Poor Mixing Zones Induce Long-Term Adaptation of E. coli Cultures in Large-Scale Bioreactors: Experimental Evidence and Mathematical Model

(2017) Alexander Nieß et al.   Frontiers in Microbiology

Bioprocess scale-up/down as integrative enabling technology: from fluid mechanics to systems biology and beyond

(2017) Frank Delvigne et al.   Microbial Biotechnology

Transcriptional response of Escherichia coli to ammonia and glucose fluctuations

(2017) Joana Danica Simen et al.   Microbial Biotechnology

Correlation between genome reduction and bacterial growth

(2016) Masaomi Kurokawa et al.   DNA RESEARCH

Strategies of isoprenoids production in engineered bacteria

(2016) Y. Li et al.   JOURNAL OF APPLIED MICROBIOLOGY

Engineering E. coli for large-scale production – Strategies considering ATP expenses and transcriptional responses

(2016) Michael Löffler et al.   METABOLIC ENGINEERING

Indispensability of Horizontally Transferred Genes and Its Impact on Bacterial Genome Streamlining

(2016) Ildikó Karcagi et al.   MOLECULAR BIOLOGY AND EVOLUTION

A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite Production

(2016) Tat-Ming Lo et al.   Cell Systems

Physiological effects of pH gradients onEscherichia coliduring plasmid DNA production

(2015) José T. Cortés et al.   BIOTECHNOLOGY AND BIOENGINEERING

Production of Biopharmaceuticals in E. coli: Current Scenario and Future Perspectives

(2015) Mohammed N. Baeshen et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Aerobic expression ofVitreoscillahemoglobin efficiently reduces overflow metabolism inEscherichia coli

(2014) Tania E. Pablos et al.   Biotechnology Journal

Cell factories for insulin production

(2014) Nabih A Baeshen et al.   Microbial Cell Factories

The metabolic cost of flagellar motion inPseudomonas putida KT2440

(2013) Esteban Martínez-García et al.   ENVIRONMENTAL MICROBIOLOGY

Positive and negative selection using the tetA-sacB cassette: recombineering and P1 transduction in Escherichia coli

(2013) Xin-tian Li et al.   NUCLEIC ACIDS RESEARCH

A two-compartment bioreactor system made of commercial parts for bioprocess scale-down studies: Impact of oscillations on Bacillus subtilis fed-batch cultivations

(2011) Stefan Junne et al.   Biotechnology Journal

An industrial perspective on bioreactor scale-down: What we can learn from combined large-scale bioprocess and model fluid studies

(2011) Henk Noorman   Biotechnology Journal

Scale-down of penicillin production in Penicillium chrysogenum

(2011) Lodewijk P. de Jonge et al.   Biotechnology Journal

ATP Requirement for Acidic Resistance in Escherichia coli

(2011) Y. Sun et al.   JOURNAL OF BACTERIOLOGY

Quantification of metabolic limitations during recombinant protein production in Escherichia coli

(2011) Jan Heyland et al.   JOURNAL OF BIOTECHNOLOGY

Real-time monitoring of metabolic shift and transcriptional induction of yciG::luxCDABE E. coli reporter strain to a glucose pulse of different concentrations

(2011) Sirichai Sunya et al.   JOURNAL OF BIOTECHNOLOGY

Scale-up of microbial processes: Impacts, tools and open questions

(2011) R. Takors   JOURNAL OF BIOTECHNOLOGY

Genome-derived minimal metabolic models for Escherichia coli MG1655 with estimated in vivo respiratory ATP stoichiometry

(2010) Hilal Taymaz-Nikerel et al.   BIOTECHNOLOGY AND BIOENGINEERING

Scale-down simulators for metabolic analysis of large-scale bioprocesses

(2010) Peter Neubauer et al.   CURRENT OPINION IN BIOTECHNOLOGY

Fast dynamic response of the fermentative metabolism ofEscherichia colito aerobic and anaerobic glucose pulses

(2009) Alvaro R. Lara et al.   BIOTECHNOLOGY AND BIOENGINEERING

Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coli

(2009) Frank Delvigne et al.   Microbial Cell Factories

Optimization of an E. coli L-rhamnose-inducible expression vector: test of various genetic module combinations

(2008) Angelika Wegerer et al.   BMC BIOTECHNOLOGY