Article
Biochemistry & Molecular Biology
Nikolett Bodnar, Katalin Varnagy, Lajos Nagy, Gizella Csire, Csilla Kallay
Summary: The study investigated the impact of ascorbic acid on the metal-catalyzed oxidation of a human prion protein model peptide. The results showed that the presence of ascorbic acid affected the formation of copper(II) complexes and the oxidation of methionine and histidine amino acids in the peptide.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Yifan Meng, Elumalai Gnanamani, Richard N. Zare
Summary: Using room-temperature water microdroplets and applying negative high voltage at the sprayer source, toluene can react with CO2 to form phenylacetic acid in one step without any catalyst. The chemical components of these microdroplets are identified by mass spectrometry, and product structures are confirmed by tandem mass spectrometry. Mechanistic studies reveal that benzyl radicals formed from hydroxyl radicals at the water microdroplet interface drive these carboxylation reactions. This water microdroplet chemistry is general, enabling the activation and subsequent carboxylation of aryl alpha-C-H groups.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Physical
Fengqian Zhao, Jian-Xing Xu, Fu-Peng Wu, Xiao-Feng Wu
Summary: This communication describes a copper-catalyzed selectivity-reversed borocarbonylation. In the presence of CuOAc/BuPAd2 catalytic system, a series of unactivated terminal alkenes were transformed into β-boryl ketones in moderate yields, using sterically hindered alkyl bromides as the electrophiles.
JOURNAL OF CATALYSIS
(2023)
Article
Chemistry, Analytical
Yi Yang, Fan Zhang, Yutian Gan, Hui-Min Zhang, Peilu Liu, Anna Mah, Lynn Gennaro, Christian Schoneich
Summary: Characterization of antibody charge heterogeneity is crucial in antibody drug development. This study presents a new workflow combining untargeted and targeted analyses to identify and characterize the induced acidic variants in a highly oxidized IgG1 antibody. The study identified 28 site-specific oxidation products as the sources of the induced charge heterogeneity and provides new insights into understanding the acidic charge heterogeneity of antibody drugs. The characterization workflow presented in this study can be widely applied as a platform approach in the biotechnology industry for in-depth characterization of antibody charge variants.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi Yang, Fan Zhang, Yutian Gan, Hui-Min Zhang, Peilu Liu, Anna Mah, Lynn Gennaro, Christian Schoeneich
Summary: Characterization of antibody charge heterogeneity is crucial for antibody drug development. A correlation between acidic charge heterogeneity and metal catalyzed oxidation has been observed, but the acidic variants induced by metal-catalyzed oxidation are not fully understood. This study presents a new characterization workflow combining untargeted and targeted analyses to identify and characterize acidic variants in a highly oxidized IgG1 antibody. The results provide new insights into the acidic charge heterogeneity of antibody drugs and offer a platform for in-depth characterization of antibody charge variants.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Fu-Peng Wu, Xiao-Feng Wu
Summary: This study reports a borylative methylation reaction catalyzed by CuH and CuBpin, yielding moderate to good yields of alkyl boranes. In this cooperative system, CuH reacts with alkyl iodide faster than CuBpin.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Polymer Science
Marco Borsari, Niccolo Braidi, Mirko Buffagni, Franco Ghelfi, Francesca Parenti, Nicola Porcelli, Gaia Serafini, Abdirisak Ahmed Isse, Luisa Bonifaci, Gianfranco Cavalca, Aldo Longo, Ida Morandini, Nicolo Pettenuzzo
Summary: ARGET ATRP of styrene was conducted using various carbonates as reducing agents, with or without ascorbic acid (AA), in a solvent mixture of EtOAc and EtOH. The system showed high yields and low dispersities with low amounts of catalyst and moderate reaction times, with the Cl-ATRP system providing excellent results. NMR spectroscopy and kinetic analyses confirmed the livingness of the final polymer chains.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Xiaonan Liu, Tingting Zhang, Xiangyang Li, Shiyun Ai, Shuang Zhou
Summary: Cu-N-C materials combined with gold nanoparticles were used to construct a non-enzymatic electrochemical sensor for nitrite detection. The sensor exhibited excellent performance with a wide linear range, low detection limit, superior selectivity, and reproducibility. This study provides an innovative strategy for designing highly sensitive electrochemical sensors and broadens the application of Cu-N-C materials in the field of food safety.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Physical
Fengqian Zhao, Patrizio Russo, Raffaella Mancuso, Bartolo Gabriele, Xiao-Feng Wu
Summary: A copper-catalyzed carbonylation of alkyl iodides with phenols is reported, yielding corresponding esters in good yields. Aliphatic alcohols are also suitable partners in this reaction.
JOURNAL OF CATALYSIS
(2022)
Article
Chemistry, Multidisciplinary
Fu-Peng Wu, Yang Yuan, Jiawang Liu, Xiao-Feng Wu
Summary: An unprecedented and challenging defluorinative carbonylation reaction was achieved with the first example of defluorinative carbonylative coupling established using a Pd/Cu cooperative catalyst system. This methodology, using gem-difluoroalkenes and aryl iodides as substrates, provides flexible and facile access to privileged alpha-fluorochalcones under mild reaction conditions in moderate-to-excellent yields. Mechanistic studies demonstrated that transmetalation between palladium and copper intermediates is a crucial step in the catalytic cycle.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Fu-Peng Wu, Hui-Qing Geng, Xiao-Feng Wu
Summary: A copper-catalyzed boroaminomethylation of olefins with CO as the C1 source via the mechanism of a carbene insertion into the N-H bond is reported in this study. This method affords valuable gamma-boryl amines from four inexpensive readily available chemicals.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Fu-Peng Wu, Xiao-Feng Wu
Summary: Regioselective transformation is achieved in copper-catalyzed regiodivergent borocarbonylation of imines with alkyl iodides, with different ligands controlling the regioselectivity to produce either alpha-amino ketones or alpha-boryl amides selectively.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Fu-Peng Wu, Yang Yuan, Xiao-Feng Wu
Summary: A novel copper-catalyzed carbonylative trifluoromethylation of unactivated alkenes has been developed, allowing the preparation of a broad range of beta-trifluoromethylated carboxylic acid derivatives in moderate to excellent yields with excellent regioselectivity. It is worth noting that even the simplest olefin, ethylene gas, can be directly applied to obtain beta-trifluoromethylated amides and esters. This transformation represents the first example of carbonylative trifluoromethylation of alkenes.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Youcan Zhang, Zhi-Peng Bao, Chang-Sheng Kuai, Xiao-Feng Wu
Summary: Here, we have developed a direct and effective synthesis strategy for sulfur-containing carboxylic acid derivatives through copper/visible light catalyzed ring-opening carbonylation of sulfonium salts. The protocol utilizes photoredox to selectively cleave the C(sp3)-S bond of sulfonium salts, while sequentially functionalizing to form vicinal C-C and C-X (X = O or N) bonds in the presence of carbon monoxide and nucleophiles. Various substrates were successfully transformed into desired carbonylated products with moderate to good yields, displaying good functional group tolerance and excellent chemoselectivity. Importantly, this approach can be easily extended to late-stage modification of bioactive molecules.
JOURNAL OF CATALYSIS
(2023)
Review
Chemistry, Physical
Riccardo Giovanelli, Giulio Bertuzzi, Marco Bandini
Summary: The utilization of CO2 as an efficient and environmentally friendly chemical analogue of CO is becoming a solid reality in the chemical scenario. CO2-based carbonylations have started paralleling the more consolidated carboxylation procedures, opening new horizons and perspectives in the utilization of carbon dioxide as an organic C1-containing building block. The advent of efficient and site-selective metal-catalyzed protocols for the fixation of CO2 into organic scaffolds, under controlled reductive conditions, contributed substantially to the development of robust, efficient, and convenient protocols. In the present Review article, a collection of the most recent examples of metal-catalyzed CO2-based carbonylations is documented with a particular emphasis on mechanistic aspects.
Article
Chemistry, Multidisciplinary
Geoffrey A. Heinzl, Lo Lai, V. Ashutosh Rao
PHARMACEUTICAL RESEARCH
(2019)
Article
Chemistry, Analytical
Kui Yang, Asha Hewarathna, Ilan Geerlof-Vidaysky, V. Ashutosh Rao, Connie Gryniewicz-Ruzicka, David Keire
ANALYTICAL CHEMISTRY
(2019)
Article
Medicine, Research & Experimental
Shen Luo, Keisha Melodi McSweeney, Tao Wang, Silvia M. Bacot, Gerald M. Feldman, Baolin Zhang
Editorial Material
Oncology
V. Ashutosh Rao
Article
Multidisciplinary Sciences
Elliot Rosen, Dmitry Kryndushkin, Baikuntha Aryal, Yanira Gonzalez, Leena Chehab, Jennifer Dickey, V. Ashutosh Rao
Article
Cell Biology
Thomas G. Biel, Baikuntha Aryal, Michael H. Gerber, Jose G. Trevino, Naoko Mizuno, V. Ashutosh Rao
CELL DEATH & DISEASE
(2020)
Review
Pharmacology & Pharmacy
Julianne D. Twomey, Shen Luo, Alexis Q. Dean, William P. Bozza, Ancy Nalli, Baolin Zhang
DRUG RESISTANCE UPDATES
(2020)
Review
Pharmacology & Pharmacy
Alexis Q. Dean, William P. Bozza, Julianne D. Twomey, Shen Luo, Ancy Nalli, Baolin Zhang
Summary: ACE2 plays a dual role in COVID-19, depending on the stage of infection and coexisting diseases in patients.
DRUG DISCOVERY TODAY
(2021)
Article
Chemistry, Multidisciplinary
Shen Luo, Baolin Zhang
Summary: This study aims to establish a benchmark glycan profile for commercially approved therapeutic monoclonal antibodies. By comparing the glycosylation data from over 150 antibodies produced by various expression systems, the study identified nine prevalent glycan epitopes and established a benchmark glycan profile. The findings have significant implications for therapeutic antibody development, quality control, and regulatory compliance.
PHARMACEUTICAL RESEARCH
(2023)
Review
Medicine, Research & Experimental
Alexis Q. Dean, Shen Luo, Julianne D. Twomey, Baolin Zhang
Summary: ADCs are a rapidly expanding class of biotherapeutics that utilize antibodies to selectively deliver cytotoxic drugs to the tumor site. While there are several ADCs approved by the FDA and many more in clinical trials, challenges remain in expanding their therapeutic index. Advances in manufacturing technology and new bioconjugation platforms are shaping the future development of ADCs.
Meeting Abstract
Oncology
Yong Zhang, Svetlana Petrovskaya, Emma C. Scott, Luis Santana-Quintero, Tigran Ghazanchyan, Amy Rosenburg, V. Ashutosh Rao, Gideon M. Blumenthal, Marc Theoret, Richard Pazdur, Julia A. Beaver, Dickran Kazandjian
Meeting Abstract
Oncology
Elliot T. Rosen, Dmitry Kryndushkin, Baikuntha Aryal, Yanira Gonzalez, Leena Chehab, Jennifer Dickey, Steven Mog, V. Ashutosh Rao
Meeting Abstract
Oncology
Emma C. Scott, Dickran Kazandjian, Luis Santana-Quintero, Tigran Ghazanchyan, Svetlana Petrovskaya, Yong Zhang, Amy Rosenberg, V. Ashutosh Rao, Jennifer L. Marte, Gideon M. Blumenthal, Marc R. Theoret, Richard Pazdur, James L. Gulley, Julia A. Beaver
Meeting Abstract
Oncology
Baikuntha P. Aryal, Jean-Pierre Gillet, V. Ashutosh Rao
Article
Clinical Neurology
Annemieke Aartsma-Rus, Jennifer Morgan, Pallavi Lonkar, Hendrik Neubert, Jane Owens, Michael Binks, Marisol Montolio, Rahul Phadke, Nicole Datson, Judith Van Deutekom, Glenn E. Morris, V. Ashutosh Rao, Eric P. Hoffman, Francesco Muntoni, Virginia Arechavala-Gomeza
JOURNAL OF NEUROMUSCULAR DISEASES
(2019)
Correction
Biochemistry & Molecular Biology
Siew Chin Chan, Chih-Wei Tung, Chia-Wei Lin, Yun-Shiuan Tung, Po-Min Wu, Pei-Hsun Cheng, Chuan-Mu Chen, Shang-Hsun Yang
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Suyuan Liu, Meiling Tan, Jiangxue Cai, Chenxuan Li, Miaoxin Yang, Xiaoxiao Sun, Bin He
Summary: This study reveals that the antibiotic doxycycline effectively inhibits NLRP3 inflammasome activation by targeting mitochondrial translation and mtDNA synthesis, offering potential for the treatment of NLRP3-related diseases.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
Article
Biochemistry & Molecular Biology
Hao Liu, Nana Li, Ge Kuang, Xia Gong, Ting Wang, Jun Hu, Hui Du, Minxuan Zhong, Jiashi Guo, Yao Xie, Yang Xiang, Shengwang Wu, Yiling Yuan, Xinru Yin, Jingyuan Wan, Ke Li
Summary: Protectin D1 (PTD1) improves hepatic steatosis, inflammation and fibrosis in a NASH mouse model by inhibiting the activation of TLR4 downstream signaling pathway, possibly through upregulation of IRAK-M expression, suggesting a potential new treatment for NASH.
FREE RADICAL BIOLOGY AND MEDICINE
(2024)
