VHUT-cryo-FIB, a method to fabricate frozen hydrated lamellae from tissue specimens for in situ cryo-electron tomography

Automated cryo-lamella preparation for high-throughput in-situ structural biology

(2020) Genevieve Buckley et al.   JOURNAL OF STRUCTURAL BIOLOGY

Fully automated, sequential focused ion beam milling for cryo-electron tomography

(2020) Tobias Zachs et al.   eLife

Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision

(2020) Wojciech Wietrzynski et al.   eLife

Cryo‐FIB preparation of whole cells and tissue for cryo‐TEM: use of high‐pressure frozen specimens in tubes and planchets

(2020) D.A.M. DE WINTER et al.   JOURNAL OF MICROSCOPY

Cryo‐FIB‐lift‐out: practically impossible to practical reality

(2020) CHRISTOPHER DAVID PARMENTER et al.   JOURNAL OF MICROSCOPY

Using focus ion beam to prepare crystal lamella for electron diffraction

(2019) Heng Zhou et al.   JOURNAL OF STRUCTURAL BIOLOGY

A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue

(2019) Miroslava Schaffer et al.   NATURE METHODS

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

(2019) Sergey Gorelick et al.   eLife

Mind the gap: Micro-expansion joints drastically decrease the bending of FIB-milled cryo-lamellae

(2019) Georg Wolff et al.   JOURNAL OF STRUCTURAL BIOLOGY

Real-time cryo-electron microscopy data preprocessing with Warp

(2019) Dimitry Tegunov et al.   NATURE METHODS

Mechanism of ribosome stalling during translation of a poly(A) tail

(2019) Viswanathan Chandrasekaran et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Machining protein microcrystals for structure determination by electron diffraction

(2018) Helen M. E. Duyvesteyn et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

emClarity: software for high-resolution cryo-electron tomography and subtomogram averaging

(2018) Benjamin A. Himes et al.   NATURE METHODS

MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy

(2017) Shawn Q Zheng et al.   NATURE METHODS

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms

(2017) Muyuan Chen et al.   NATURE METHODS

The structure of the COPI coat determined within the cell

(2017) Yury S Bykov et al.   eLife

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

(2017) Lara Eid et al.   Jove-Journal of Visualized Experiments

ICON: 3D reconstruction with ‘missing-information’ restoration in biological electron tomography

(2016) Yuchen Deng et al.   JOURNAL OF STRUCTURAL BIOLOGY

An improved cryo-FIB method for fabrication of frozen hydrated lamella

(2016) Jianguo Zhang et al.   JOURNAL OF STRUCTURAL BIOLOGY

Making the practically impossible “Merely difficult”-Cryogenic FIB lift-out for “Damage free” soft matter imaging

(2016) Christopher D.J. Parmenter et al.   MICROSCOPY RESEARCH AND TECHNIQUE

Visualizing the molecular sociology at the HeLa cell nuclear periphery

(2016) J. Mahamid et al.   SCIENCE

A focused ion beam milling and lift-out approach for site-specific preparation of frozen-hydrated lamellas from multicellular organisms

(2015) Julia Mahamid et al.   JOURNAL OF STRUCTURAL BIOLOGY

CTFFIND4: Fast and accurate defocus estimation from electron micrographs

(2015) Alexis Rohou et al.   JOURNAL OF STRUCTURAL BIOLOGY

Structural analysis of multicellular organisms with cryo-electron tomography

(2015) Jan Harapin et al.   NATURE METHODS

In situ structural analysis of Golgi intracisternal protein arrays

(2015) Benjamin D. Engel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Practical workflow for cryo focused-ion-beam milling of tissues and cells for cryo-TEM tomography

(2013) Chyongere Hsieh et al.   JOURNAL OF STRUCTURAL BIOLOGY

A site-specific focused-ion-beam lift-out method for cryo Transmission Electron Microscopy

(2012) Stefano Rubino et al.   JOURNAL OF STRUCTURAL BIOLOGY

RELION: Implementation of a Bayesian approach to cryo-EM structure determination

(2012) Sjors H.W. Scheres   JOURNAL OF STRUCTURAL BIOLOGY

Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography

(2012) A. Rigort et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Micromachining tools and correlative approaches for cellular cryo-electron tomography

(2010) Alexander Rigort et al.   JOURNAL OF STRUCTURAL BIOLOGY

The making of frozen-hydrated, vitreous lamellas from cells for cryo-electron microscopy

(2010) Michael F. Hayles et al.   JOURNAL OF STRUCTURAL BIOLOGY

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-A resolution

(2010) J.-P. Armache et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA