In vitro antimicrobial, antiradical, anticancer evaluation, and phytochemical contents of endemic Scorzonera semicana DC

In the present study, antimicrobial, antiradical, and anticancer properties with phytochemical compounds of endemic Scorzonera semicana DC., which grown in Elazig (from Turkey) around and consumed as a vegetable, were investigated. The antiradical properties of extracts were determined via DPPH, ABTS, OH radical scavenging activities, and their antimicrobial properties were determined against several microorganisms. Anticancer properties of this plant were determined by MTT assay on the HCT-116, LNCaP, and MCF-7 under in vitro conditions. It was determined that S. semicana water (94.81%) and methanol (98.87%) extracts for scavenging of ABTS (BHT, 93.24%); water, ethanol, and methanol extracts (84.97%, 84.29%, and 84.62%, respectively) for scavenging of OH radicals showed higher activity than BHT (75.77%). It was observed that flavonoid (5,555.34 mu g CE/g), proanthocyanidin (1,860.78 mu g CE/g), and phenolic (134.89 mg GAE/g) contents were found to be highest in S. semicana methanol extract. All extracts had higher antimicrobial activity than standard antibiotics against Listeria monocytogenes. This plant showed very effective anticancer activity increasing dose-dependently on LNCaP, MCF-7, and HCT-116 cancer cell lines. Practical applications In Turkey, Scorzonera semicana DC. is cultivated and consumed as vegetables, and the roots, fresh shoots of this species are consumed by the name of yemlik as feed or fresh in Anatolia. This study investigated its antiradical, antimicrobial, anticancer, phytochemical properties and is first report about in vitro biological properties of S. semicana. According to our results, S. semicana most effective for ABTS and OH radical scavenging activity and show very effective anticancer activity on LNCaP, MCF-7, HCT-116 cells.

Automated summary

The present study investigated the antimicrobial, antiradical, and anticancer properties of endemic Scorzonera semicana DC. from Turkey, showing promising results in scavenging free radicals and inhibiting cancer cell lines. The plant exhibited higher activity in all tested aspects compared to standard antibiotics and antioxidants, making it a potential source for pharmaceutical and nutritional applications.