Detection of genome edits in plants—from editing to seed

Development and application of sensitive, specific and rapid CRISPR‐Cas13‐based diagnosis

(2021) David Gao et al.   JOURNAL OF MEDICAL VIROLOGY

Advanced domestication: harnessing the precision of gene editing in crop breeding

(2021) Wendy J. Lyzenga et al.   PLANT BIOTECHNOLOGY JOURNAL

In planta Genome Editing in Commercial Wheat Varieties

(2021) Yuelin Liu et al.   Frontiers in Plant Science

5Gs for crop genetic improvement

(2020) Rajeev K Varshney et al.   CURRENT OPINION IN PLANT BIOLOGY

Plant genome editing and the relevance of off-target changes

(2020) Nathaniel Graham et al.   PLANT PHYSIOLOGY

A GRF–GIF chimeric protein improves the regeneration efficiency of transgenic plants

(2020) Juan M. Debernardi et al.   NATURE BIOTECHNOLOGY

A Sensitive, Highly Specific Novel Isothermal Amplification Method Based on Single-Nucleotide Polymorphism for the Rapid Detection of Salmonella Pullorum

(2020) Haiyan Shen et al.   Frontiers in Microbiology

Overexpression of the Transcription Factor GROWTH-REGULATING FACTOR5 Improves Transformation of Dicot and Monocot Species

(2020) Jixiang Kong et al.   Frontiers in Plant Science

Genome editing for plant research and crop improvement

(2020) Xiangqiang Zhan et al.   Journal of Integrative Plant Biology

Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR

(2020) Cheng Peng et al.   Frontiers in Plant Science

Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants

(2020) Dominik Modrzejewski et al.   Frontiers in Plant Science

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Detection and Identification of Genome Editing in Plants: Challenges and Opportunities

(2019) Lutz Grohmann et al.   Frontiers in Plant Science

Evaluation of Methods to Assess in vivo Activity of Engineered Genome-Editing Nucleases in Protoplasts

(2019) Satya Swathi Nadakuduti et al.   Frontiers in Plant Science

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

(2019) Kunling Chen et al.   Annual Review of Plant Biology

An RNA-Guided Cas9 Nickase-Based Method for Universal Isothermal DNA Amplification

(2019) Ting Wang et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

CRISPR-Cas9 Editing in Maize: Systematic Evaluation of Off-target Activity and Its Relevance in Crop Improvement

(2019) Joshua Young et al.   Scientific Reports

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

Methods of Evaluating the Efficiency of CRISPR/Cas Genome Editing

(2019) N. A. Lomov et al.   MOLECULAR BIOLOGY

Genetic strategies for improving crop yields

(2019) Julia Bailey-Serres et al.   NATURE

Optimisation of droplet digital PCR for determining copy number variation of α-gliadin genes in mutant and gene-edited polyploid bread wheat

(2019) Aurélie Jouanin et al.   JOURNAL OF CEREAL SCIENCE

Plant gene editing through de novo induction of meristems

(2019) Michael F. Maher et al.   NATURE BIOTECHNOLOGY

Handheld isothermal amplification and electrochemical detection of DNA in resource-limited settings

(2018) Maria-Nefeli Tsaloglou et al.   ANALYTICAL BIOCHEMISTRY

Smartphone-Based Mobile Detection Platform for Molecular Diagnostics and Spatiotemporal Disease Mapping

(2018) Jinzhao Song et al.   ANALYTICAL CHEMISTRY

The Effect of Single Mismatches on Primer Extension

(2018) Nick A. Rejali et al.   CLINICAL CHEMISTRY

Scarless genome editing: progress towards understanding genotype–phenotype relationships

(2018) Gregory L. Elison et al.   CURRENT GENETICS

Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations

(2018) Jesse J. Salk et al.   NATURE REVIEWS GENETICS

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

(2018) Choun-Sea Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Biolistic-delivery-based transient CRISPR/Cas9 expression enables in planta genome editing in wheat

(2018) Haruyasu Hamada et al.   Scientific Reports

Effect of Hybridization on Somatic Mutations and Genomic Rearrangements in Plants

(2018) Tufail Bashir et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

APPLICATION OF DNA AND PROTEIN-BASED DETECTION METHODS IN AGRICULTURAL BIOTECHNOLOGY

(2018) Clara M Alarcon et al.   JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection

(2018) Wenhua Zhou et al.   Nature Communications

A multi-purpose toolkit to enable advanced genome engineering in plants

(2017) Tomas Cermak et al.   PLANT CELL

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

Single-Step qPCR and dPCR Detection of Diverse CRISPR-Cas9 Gene Editing Events in Vivo

(2017) Micol Falabella et al.   G3-Genes Genomes Genetics

A technical application of quantitative next generation sequencing for chimerism evaluation

(2016) Michelangelo Aloisio et al.   Molecular Medicine Reports

Nuclease Target Site Selection for Maximizing On-target Activity and Minimizing Off-target Effects in Genome Editing

(2016) Ciaran M Lee et al.   MOLECULAR THERAPY

Digital PCR to assess gene-editing frequencies (GEF-dPCR) mediated by designer nucleases

(2016) Ulrike Mock et al.   Nature Protocols

Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation

(2016) Keith Lowe et al.   PLANT CELL

A Digital PCR-Based Method for Efficient and Highly Specific Screening of Genome Edited Cells

(2016) Scott D. Findlay et al.   PLoS One

Systematic quantification of HDR and NHEJ reveals effects of locus, nuclease, and cell type on genome-editing

(2016) Yuichiro Miyaoka et al.   Scientific Reports

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis

(2015) Zhengjing Zhang et al.   PLANT CELL REPORTS

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Off-target Effects in CRISPR/Cas9-mediated Genome Engineering

(2015) Xiao-Hui Zhang et al.   Molecular Therapy-Nucleic Acids

Anchored multiplex PCR for targeted next-generation sequencing

(2014) Zongli Zheng et al.   NATURE MEDICINE

Detecting ultralow-frequency mutations by Duplex Sequencing

(2014) Scott R Kennedy et al.   Nature Protocols

Using next-generation sequencing to detect mutations endowing resistance to pesticides: application to acetolactate-synthase (ALS)-based resistance in barnyard grass, a polyploid grass weed

(2014) Christophe Délye et al.   PEST MANAGEMENT SCIENCE

RNA-seq differential expression studies: more sequence or more replication?

(2013) Yuwen Liu et al.   BIOINFORMATICS

Promise and issues of genetically modified crops

(2013) Hao Chen et al.   CURRENT OPINION IN PLANT BIOLOGY

Fast, reliable and low cost user-developed protocol for detection, quantification and genotyping of hepatitis C virus

(2013) Katarina Davalieva et al.   JOURNAL OF VIROLOGICAL METHODS

Detection of ultra-rare mutations by next-generation sequencing

(2012) M. W. Schmitt et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accurate sampling and deep sequencing of the HIV-1 protease gene using a Primer ID

(2011) C. B. Jabara et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Detection and quantification of rare mutations with massively parallel sequencing

(2011) I. Kinde et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TAL Effectors: Customizable Proteins for DNA Targeting

(2011) A. J. Bogdanove et al.   SCIENCE