Article
Food Science & Technology
Jin-Young Han, Sang-Hyun Park, Dong-Hyun Kang
Summary: The study evaluated the decontamination effect and inactivation mechanism of plasma bubble-activated water (PBAW) treatment against foodborne pathogens. It found that PBAW effectively reduced the levels of pathogens on tomatoes and in wash water, and achieved this through oxidizing the cell membrane and causing a loss of membrane integrity using reactive species dissolved in PBAW.
Article
Biochemistry & Molecular Biology
Giles Grainge, Kazumi Nakabayashi, Felipe Iza, Gerhard Leubner-Metzger, Tina Steinbrecher
Summary: Limited light availability affects seed germination timing and percentage. Seed industries are exploring interventions to mitigate this risk, and one area of research is water treated with gas plasma. However, there is limited understanding of how this solution influences seed dormancy mechanisms and photoreceptor-driven germination. This study investigated the effects of gas plasma-treated water on seed germination under low light intensities and described the impact on key physiological regulators.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Han Du, Xiaoman Wang, Qingli Yang, Wei Wu
Summary: Quantum dots show great potential in detecting foodborne pathogens, providing rapid and sensitive monitoring of target bacteria. Compared to other methods, quantum dots are more cost-effective, and detection strategies associated with smartphones help achieve new breakthroughs.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Akbar Bahrami, Rana Delshadi, Ilaria Cacciotti, Afshin Faridi Esfanjani, Atefe Rezaei, Ozgur Tarhan, Chi Ching Lee, Elham Assadpour, Merve Tomas, Beyza Vahapoglu, Esra Capanoglu Guven, Leonard Williams, Seid Mahdi Jafari
Summary: The incorporation of antibiotics and bioactive compounds into non-toxic nanoparticles has been widely used to produce effective antimicrobial nanocarriers against foodborne pathogens. However, there are major barriers that can decrease their functions. Surface functionalization of nanocarriers through passive and active modification methods can protect them against clearance from the blood and increase their circulation time, leading to enhanced antimicrobial activities.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Review
Chemistry, Physical
Lea R. Winter, Jingguang G. Chen
Summary: This paper compares the energy requirements, CO2 emissions, and commodity prices of alternative plasma-activated ammonia synthesis with the conventional Haber-Bosch process. Economic positive externalities are evaluated for renewable energy-powered modular ammonia production, with a 6-fold improvement in plasma process efficiency projected to make it competitive. Technological prospects for achieving the thresholds are discussed, including advancements in plasma-catalyst synergy and direct reactions of N-2 with plasma-activated water.
Article
Food Science & Technology
Xiaoying Han, Yuanhui Liu, Juxin Yin, Min Yue, Ying Mu
Summary: The global burden of foodborne diseases is significant, with foodborne pathogens being the major cause of human illnesses. Microfluidic technology enables rapid, cost-effective, sensitive, and portable pathogen detection to meet on-site testing requirements. Multiplexed detection technology offers a new opportunity to ensure food safety by simultaneously detecting multiple pathogens.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Justyna Nasilowska, Aleksandra Kocot, Paulina Natalia Osuchowska, Barbara Sokolowska
Summary: High Hydrostatic Pressure (HHP) technology is considered an alternative method of food preservation, but may not be sufficient to protect food lastingly against some pathogens. HHP-induced sublethal injuries on bacteria result in changes in cell morphology and subcellular structures, with varying degrees of damage observed in different bacterial strains.
Article
Plant Sciences
Jana Jiresova, Vladimir Scholtz, Jaroslav Julak, Bozena Sera
Summary: Recently, the use of low-temperature plasmas and plasma-activated water (PAW) in biological research has gained much attention. This article reviews the composition and properties of PAW prepared by different methods, and finds no significant difference between PAW prepared by plasma treatment and that prepared by mixing the active ingredients. The research also shows that PAW promotes germination and growth of wheat grains, and effectively decontaminates bacteria and yeast.
Review
Food Science & Technology
Aswathi Soni, Jonghyun Choi, Gale Brightwell
Summary: Plasma-activated water (PAW) is generated by treating water with cold atmospheric plasma (CAP) using controllable parameters, and it has been shown to have a significant potential as a non-chemical and non-thermal intervention for bacterial inactivation, especially on food. However, the applicability and usage of PAW depend on environmental and bacterial strain-based conditions as well as cost-effectiveness.
Article
Chemistry, Analytical
Shuo Yao, Bo Pang, Yanli Fu, Xiuling Song, Kun Xu, Juan Li, Juan Wang, Chao Zhao
Summary: In this study, a quantitative assay using ICP-MS, magnetic separation, and metal nanoclusters-mediated signal amplification was developed for the simultaneous detection of multiple foodborne pathogens. The method showed a high sensitivity, being able to detect three target pathogens as low as 10 CFU/mL in a short time. It was successfully applied to the determination of target bacteria in food samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Jianying Zhao, Jing Qian, Ji Luo, Mingming Huang, Wenjing Yan, Jianhao Zhang
Summary: This study evaluated the effects of plasma-activated hydrogen peroxide solution (PAH) on the morphophysiology of Staphylococcus aureus biofilms. The results indicated that PAH significantly reduced the population of S. aureus in the biofilms, decreased the thickness and metabolic capacity of the biofilms, while increasing ROS content and DNA fragments of bacteria. PAH treatments were found to be a valuable anti-biofilm technology to reduce foodborne pathogens.
APPLIED SCIENCES-BASEL
(2021)
Article
Agronomy
Matej Holc, Peter Gselman, Gregor Primc, Alenka Vesel, Miran Mozetic, Nina Recek
Summary: In this study, it was found that treatment of alfalfa seeds with plasma improves their surface hydrophilicity, leading to potential benefits for cultivation and growth. The functionalization and etching of seed surface contribute to the regulation of seed wettability.
Article
Chemistry, Analytical
Hang You, Ming Wang, Shuai Wang, Jie Xu, Shuhao Hu, Tianhua Li, Zhenzhong Yu, Dianping Tang, Ning Gan
Summary: This research developed an ultrasensitive and specific Phage@DNAzyme signal probe for the rapid and on-site detection of virulent foodborne pathogens. The probe efficiently catalyzed a click reaction to generate a visual fluorescent signal, allowing for the quantification of target concentrations using a smartphone. The method demonstrated satisfactory sensing performances for Escherichia coli O157:H7 and Salmonella typhimurium, providing a new approach for trace level detection of pathogenic strains in foods.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Piotr Terebun, Michal Kwiatkowski, Karol Hensel, Marek Kopacki, Joanna Pawlat
Summary: The study found that using low-temperature plasma activated water can improve the germination and growth of beetroot and carrot seeds. Beetroot seeds showed a more positive effect with increasing treatment time, while carrot seeds exhibited a more significant positive effect on germination.
APPLIED SCIENCES-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Xiuli Dong, Ping Wang, Jasmine P. Darby, Yongan Tang, Christopher M. Overton, Sophia Kathariou, Ya-Ping Sun, Liju Yang
Summary: Foodborne pathogens have long been a major challenge for the food industry, and there is a critical need for new tools to control and prevent contaminations. This study demonstrated the effectiveness of carbon dots in photoinactivating Listeria monocytogenes and Salmonella cells, suggesting them as promising agents for foodborne pathogens. The study contributes to the practical application of CDots as new nanomaterial-based photodynamic inactivation agents.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Marion Delacoux des Roseaux, Shengjing Shi, Aoife M. Duff, Fiona P. Brennan, Leo Condron, John A. Finn, Karl G. Richards, Maureen O'Callaghan, Tim J. Clough
Summary: Multispecies grasslands can alter nitrogen cycling processes and affect dry matter yields, plant N uptake, soil N2O emissions, and genes encoding nitrifier and denitrifier N transformations. Urine application significantly increased dry matter yields and plant N uptake, but had minimal effect on soil inorganic-N concentrations.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2022)
Article
Agronomy
R. M. Murphy, K. G. Richards, D. J. Krol, A. W. Gebremichael, L. Lopez-Sangil, J. Rambaud, N. Cowan, G. J. Lanigan, M. Saunders
Summary: This study investigates the spatial and temporal variability of N2O emissions using both static chambers (CH) and eddy covariance (EC) techniques. It finds that hotspots and hot moments of N2O emissions are sensitive to changes in agricultural management and weather, making accurate quantification difficult. The study also suggests that Bayesian statistics are an appropriate method for estimating realistic means and ranges of uncertainty for CH flux datasets.
AGRICULTURAL AND FOREST METEOROLOGY
(2022)
Article
Agriculture, Multidisciplinary
Rachael M. Murphy, Matthew Saunders, Karl G. Richards, Dominika J. Krol, Amanuel W. Gebremichael, James Rambaud, Nicholas Cowan, Gary J. Lanigan
Summary: This study quantified field scale N2O emissions from grazed pastures under rotational grazing management using eddy covariance (EC) and static chamber techniques. The results showed that the EC technique was more accurate in quantifying N2O emissions, while static chamber measurements had higher uncertainty due to small chamber sample size per treatment and highly variable N2O flux measurements over space and time. Nitrous oxide emissions from grazing were mainly derived from animal excreta, highlighting the importance of considering different nitrogen sources when quantifying N2O-N losses.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Agriculture, Multidisciplinary
Fiona C. Anderson, Timothy J. Clough, Leo M. Condron, Karl G. Richards, Camille Rousset
Summary: This study examined the influence of phosphorus (P) fertility on nitrous oxide (N2O) emissions from grazed pasture systems. The researchers found that increasing P fertility enhanced soil microbial biomass P, leading to increased N2O emissions. However, the emissions were primarily driven by nitrate additions, with relatively small contributions from denitrification. Glucose addition further increased N2O emissions, and the addition of P fertiliser increased emission factors, suggesting that N2O emissions will increase with P fertilisation if carbon limitation is overcome.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2023)
Article
Environmental Sciences
Rajat Nag, Lauren Russell, Stephen Nolan, Agathe Auer, Bryan K. Markey, Paul Whyte, Vincent O'Flaherty, Declan Bolton, Owen Fenton, Karl G. Richards, Enda Cummins
Summary: Farmyard manure and slurry, as well as anaerobic digestate, are valuable soil conditioners providing essential nutrients for plant growth, but they may also pose microbial health risks to humans. A quantitative microbial risk assessment model was developed to investigate potential human exposure to pathogens post-application, revealing low risk probabilities in most scenarios.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Green & Sustainable Science & Technology
Corine Orline Nzeteu, Fabiana Coelho, Anna Christine Trego, Florence Abram, Javier Ramiro-Garcia, Lara Paulo, Vincent O'Flaherty
Summary: The efficient production of caproic acid can be achieved by manipulating the inoculum and adjusting the concentration and ratio of lactic acid and butyric acid. A threshold concentration of lactic acid to butyric acid was found, above or below which the selectivity for caproic acid is reduced.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Soil Science
C. Deveautour, P. A. Rojas-Pinzon, M. Veloso, J. Rambaud, A. M. Duff, D. Wall, R. Carolan, L. Philippot, K. G. Richards, V O'Flaherty, F. Brennan
Summary: This study evaluated the impact of abiotic and biotic factors on potential denitrification and identified soil physico-chemical properties and prokaryotic community composition as key factors in predicting potential denitrification. Soil phosphorus content was equally important in predicting potential denitrification.
SOIL BIOLOGY & BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Eoin McAleer, Catherine Coxon, Per-Erik Mellander, Jim Grant, Karl Richards
Summary: This study examines the environmental loss of nitrogen in agricultural landscapes and its consequences, and investigates the effectiveness of mitigation strategies to control nitrate contamination. The research finds that agronomic, meteorological, and hydrogeological factors significantly affect nitrate concentrations in groundwater and streams, emphasizing the need to minimize nitrogen applications in areas with high risk.
Article
Biochemical Research Methods
Kevin McDonnell, Florence Abram, Enda Howley
Summary: This study focuses on using machine learning models to identify amino acids in mass spectrometry spectra and proposes a new encoding module. By evaluating the proposed model in real and artificial data with different noise and missing peptide peaks, it is found that the model performs best among others. The study also discusses the inclusion of additional features and the issue with using AUC as a metric in the encoding modules.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Environmental Sciences
Parvin Hasani Zadeh, Fernando G. Fermoso, Gavin Collins, Antonio Serrano, Simon Mills, Florence Abram
Summary: Harnessing microbial capabilities for metal recovery from secondary waste sources is an eco-friendly and sustainable approach. Soluble microbial products (SMP) and extracellular polymeric substances (EPS) are important for remediation and recovery of metals. Microorganisms can regulate the content and composition of EPS and SMP in response to metal stress to increase their capacity for metal binding. Investigating the metal binding affinity of these compounds under metal stress conditions is crucial for selective metal recovery applications.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Soil Science
Matthias Waibel, Kevin McDonnell, Maria Tuohy, Sally Shirran, Sylvia Synowsky, Barry Thornton, Eric Paterson, Fiona Brennan, Florence Abram
Summary: Soil organic matter (SOM) is complex and important for providing nutrients to plants. Understanding SOM utilisation processes can improve fertiliser management for plant growth and reduce environmental losses. Metaproteomics can characterise protein profiles and provide insights into SOM microbial decomposition mechanisms. This study applied different extraction methods to recover SOM with different characteristics in two soil types and analysed peptide identification using liquid chromatography and tandem mass spectrometry. The results showed contrasting protein and humic substance content in different extracts, and diverse peptide hits associated with Proteobacteria and derived taxa. This work suggests areas for optimisation in chromatography and mass spectrometry to characterise SOM-associated metaproteomes.
EUROPEAN JOURNAL OF SOIL SCIENCE
(2023)
Article
Environmental Sciences
Aishwarya Ray, Patrick Forrestal, Chikere Nkwonta, Niharika Rahman, Pilar Byrne, Martin Danaher, Karl Richards, Sean Hogan, Enda Cummins
Summary: NBPT is an efficient urease inhibitor that can reduce ammonia loss from urea fertiliser. Its use not only helps address environmental issues such as soil acidification, eutrophication, and global warming, but also benefits farmers by reducing reliance on expensive nitrate fertiliser. The present study concludes that NBPT presents negligible risk to human health under the studied conditions.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Kevin McDonnell, Enda Howley, Florence Abram
Summary: Proteins are crucial for living cells and proteomics, the study of their expression, has diverse applications. Peptide identification is typically done by matching mass spectra to a protein database, but de novo methods can also be used. This study critically analyzes the use of artificial data for training and evaluating de novo peptide identification algorithms.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Kevin McDonnell, Enda Howley, Florence Abram
Summary: Proteomics is a technique used to study system-wide protein expression, which has wide ranging applications and impacts every area of biology. De novo peptide sequencing, a popular method, is improving with the integration of machine learning. This research evaluates two algorithms for de novo peptide sequencing and explores the characteristics of tandem mass spectra. The study highlights the challenges of missing cleavage sites and noise, and provides recommendations for algorithm improvements.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Food Science & Technology
Lauren Russell, Paul Whyte, Annetta Zintl, Stephen V. Gordon, Bryan Markey, Theo de Waal, Stephen Nolan, Vincent O'Flaherty, Florence Abram, Karl Richards, Owen Fenton, Declan Bolton
Summary: This study examined the survival of various bacteria in sandy and clay loam soils when applied in bovine slurry or digestate, finding that soil texture and application material did not significantly affect survival rates for most bacteria.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
Article
Biotechnology & Applied Microbiology
Lihan Huang, Nurul Hawa Ahmad, Vijay Juneja, Erika Stapp-Kamotani, Jose Gabiola, Udit Minocha, Robert Phillips, Marie Hooker, Isabel Walls, Kim Cook, James Lindsay
Summary: This study investigated the growth kinetics of foodborne pathogen B. cereus during the commercial production of liquid egg yolk using PLA(2) treatment. The results showed that B. cytotoxicus NVH391-98 can grow prolifically at 50 degrees C, with estimated minimum, optimum, and maximum temperatures of 16.7-18.5, 47.8-48.1, and 52.1-52.4 degrees C, respectively. The dynamic model was validated with isothermal curves and it was found that holding the temperature below 15 degrees C or above 55 degrees C can effectively prevent the growth of B. cytotoxicus in egg yolk.
Article
Biotechnology & Applied Microbiology
Giacomo Mantegazza, Robin Duncan, Nicolo Telesca, Giorgio Gargari, Susanna Perotti, Patrizia Riso, Simone Guglielmetti
Summary: This study investigates whether salad-associated LAB can survive the gastrointestinal transit (GIT) and contribute to the gut microbiota. The results suggest that salad-associated LAB can survive in the gastrointestinal tract, but their quantity does not significantly alter the composition of the gut microbiota.
Article
Biotechnology & Applied Microbiology
Itsaso Baztarrika, Adrian Salazar-Sanchez, Lorena Laorden, Ilargi Martinez-Ballesteros, Rodrigo Alonso, Irati Martinez-Malaxetxebarria
Summary: The infection mechanisms of Arcobacter are still uncertain. This study found that Arcobacter isolates from food and water sources were able to adhere and invade Caco-2 cells, and carried at least two virulence markers. A. butzleri showed higher virulent activity compared to A. cryaerophilus, while A. lanthieri, A. skirrowii, and A. vitoriensis exhibited even higher adhesion and invasion values. Sewage isolates were more adherent and invasive, and had a higher gene content. The association between cadF and hecA with high adhesion capability was identified for the first time.
Article
Biotechnology & Applied Microbiology
Balaji Sundararaman, Karen Shapiro, Andrea Packham, Lauren E. Camp, Rachel S. Meyer, Beth Shapiro, Richard E. Green
Summary: Pathogenic bacteria, viruses, fungi, and protozoa can cause food and waterborne diseases, and surveillance methods are needed. In this study, CNER method was used to prepare enrichment baits for difficult-to-grow pathogens. This method allows direct sequencing of pathogens from samples.
Article
Biotechnology & Applied Microbiology
Guanghui Zhou, Pengcheng Dong, Xin Luo, Lixian Zhu, Yanwei Mao, Yunge Liu, Yimin Zhang
Summary: Interactions between bacterial species in the formation of dual-species biofilms in the food industry are affected by environmental stresses, such as temperature and pH. This study found that the combination of cold and acid stresses significantly increased biofilm formation, and different temperatures resulted in variations in the presence of specific bacterial species and the secretion of extracellular substances. The findings provide valuable insights for addressing mixed biofilms in food processing under diverse stressful conditions.
Article
Biotechnology & Applied Microbiology
Carmen Sanchez, Sonia Garde, Jose Maria Landete, Javier Calzada, Dave J. Baker, Rhiannon Evans, Arjan Narbad, Melinda J. Mayer, Marta Avila
Summary: This study sequenced and compared the genomes of bacteriophages active against C. tyrobutyricum, identifying novel species and phage proteins. A phage showed high lytic activity against C. tyrobutyricum cells and remained stable under different environmental conditions. Additionally, engineered strains successfully delivered endolysin and fluorescent protein to dairy products without affecting their technological properties.
Article
Biotechnology & Applied Microbiology
Danai Etter, Michael Biggel, Mariella Greutmann, Nicole Cernela, Sophia Johler
Summary: Bacillus cytotoxicus is a thermotolerant bacterium that is linked to rare but sometimes fatal cases of diarrheal disease. It is mostly found in dehydrated foods containing potato starch or insects and may be missed in routine screening.
Article
Biotechnology & Applied Microbiology
Tong Jin, Xiangjun Zhan, Liuxin Pang, Bo Peng, Xinpeng Zhang, Wenxiu Zhu, Baowei Yang, Xiaodong Xia
Summary: The study found that CpxAR TCS is an important regulatory mechanism for the virulence of C. sakazakii. Mutants showed weakened adhesion, invasion, and survival ability, and exhibited attenuated virulence in animal models. In addition, CpxAR TCS is involved in regulating the expression of genes associated with bacterial virulence and cellular invasion.
Article
Biotechnology & Applied Microbiology
Soo-Hwan Kim, Sang-Hyun Park, Jun-Bae Ahn, Dong-Hyun Kang
Summary: In this study, a semi continuous SHS system was developed to inactivate foodborne pathogens on radish seed surfaces and enhance the seeds' salinity tolerance. The results showed that higher temperature and more treatments led to a significant reduction in microbial count on the seed surface.
Article
Biotechnology & Applied Microbiology
Yujun Zhai, Weina Tian, Kewei Chen, Linshu Lan, Jianquan Kan, Hui Shi
Summary: This research investigates the role of flagella-related genes in the adhesion of E. coli O157:H7 and the effects of certain cations on bacterial adhesion and motility. The results demonstrate that flagellar-mediated motility is critical in the early stage of adhesion, and the presence of Fe2+, Ca2+, and Mn2+ affects the adhesion and motility of wildtype strains but not flagellar function-impaired strains. These findings provide new insights into the mechanisms of bacterial adhesion and have implications for the development of anti-adhesion agents.
Article
Biotechnology & Applied Microbiology
Mariana Marggraf, Andrea Barac, Jens Andre Hammerl, Stefan Hertwig
Summary: Isolating Yersinia enterocolitica from food is challenging due to overgrowth by other microflora and the lack of a selective medium. The authors improved the standard isolation procedure, resulting in a more sensitive method, reduced background bacteria, and time savings.
Article
Biotechnology & Applied Microbiology
Rafael D. Chaves, Samuel H. Kumazawa, Amin Mousavi Khaneghah, Veronica O. Alvarenga, Humberto M. Hungaro, Anderson S. Sant'Ana
Summary: This study investigated the susceptibility to sanitizers and biofilm-forming ability of 43 Salmonella enterica and Listeria monocytogenes strains on stainless steel surfaces. The results showed variations in susceptibility to sanitizers and biofilm resistance among different strains of the pathogens, with chlorine compounds being more effective in inactivating planktonic cells and biofilms.
Article
Biotechnology & Applied Microbiology
So Yeong Mun, Wooje Lee, Soo-Young Lee, Ji Yoon Chang, Hae Choon Chang
Summary: This study reveals that lactobacilli with a strong defense system dominate the final stage of kimchi fermentation and proposes a model for the succession mechanism of kimchi lactobacilli.