Aggregation and mobility of membrane proteins interplay with local lipid order in the plasma membrane of T cells

Nanodomains can persist at physiologic temperature in plasma membrane vesicles and be modulated by altering cell lipids

(2020) Guangtao Li et al.   JOURNAL OF LIPID RESEARCH

Lipid Rafts: Controversies Resolved, Mysteries Remain

(2020) Ilya Levental et al.   TRENDS IN CELL BIOLOGY

Clustering of the ζ-Chain Can Initiate T Cell Receptor Signaling

(2020) Yuanqing Ma et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells

(2020) Simao Coelho et al.   Science Advances

Variations in Plasma Membrane Topography Can Explain Heterogenous Diffusion Coefficients Obtained by Fluorescence Correlation Spectroscopy

(2020) Astrid Gesper et al.   Frontiers in Cell and Developmental Biology

Measuring nanoscale diffusion dynamics in cellular membranes with super-resolution STED–FCS

(2019) Erdinc Sezgin et al.   Nature Protocols

Emerging Diversity in Lipid–Protein Interactions

(2019) Valentina Corradi et al.   CHEMICAL REVIEWS

A cell topography-based mechanism for ligand discrimination by the T cell receptor

(2019) Ricardo A. Fernandes et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TCR–pMHC bond conformation controls TCR ligand discrimination

(2019) Dibyendu K. Sasmal et al.   Cellular & Molecular Immunology

Synergistic factors control kinase-phosphatase organization in B cells engaged with supported bilayers

(2019) Marcos Francisco Núñez et al.   MOLECULAR BIOLOGY OF THE CELL

Photophysical Behavior of mNeonGreen, an Evolutionarily Distant Green Fluorescent Protein

(2018) Frederik Steiert et al.   BIOPHYSICAL JOURNAL

Capturing resting T cells: the perils of PLL

(2018) Ana Mafalda Santos et al.   NATURE IMMUNOLOGY

Reconstitution of immune cell interactions in free-standing membranes

(2018) Edward Jenkins et al.   JOURNAL OF CELL SCIENCE

Membrane Ultrastructure and T Cell Activation

(2018) Johannes Pettmann et al.   Frontiers in Immunology

CD45 exclusion– and cross-linking–based receptor signaling together broaden FcεRI reactivity

(2018) James H. Felce et al.   Science Signaling

Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs

(2017) Masanao Kinoshita et al.   JOURNAL OF CELL BIOLOGY

Convergence of lateral dynamic measurements in the plasma membrane of live cells from single particle tracking and STED-FCS

(2017) B Christoffer Lagerholm et al.   JOURNAL OF PHYSICS D-APPLIED PHYSICS

Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS

(2017) Falk Schneider et al.   MOLECULAR BIOLOGY OF THE CELL

The mystery of membrane organization: composition, regulation and roles of lipid rafts

(2017) Erdinc Sezgin et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Structural determinants and functional consequences of protein affinity for membrane rafts

(2017) Joseph H. Lorent et al.   Nature Communications

Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes

(2017) Matthew B Stone et al.   eLife

Localization and Ordering of Lipids Around Aquaporin-0: Protein and Lipid Mobility Effects

(2017) Rodolfo Briones et al.   Frontiers in Physiology

HIV virions sense plasma membrane heterogeneity for cell entry

(2017) Sung-Tae Yang et al.   Science Advances

A Cholesterol-Based Allostery Model of T Cell Receptor Phosphorylation

(2016) Mahima Swamy et al.   IMMUNITY

Lipid rafts in immune signalling: current progress and future perspective

(2016) Pallavi Varshney et al.   IMMUNOLOGY

Super-resolution Microscopy Reveals Compartmentalization of Peroxisomal Membrane Proteins

(2016) Silvia Galiani et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Remodeling of the postsynaptic plasma membrane during neural development

(2016) Karolina Tulodziecka et al.   MOLECULAR BIOLOGY OF THE CELL

Initiation of T cell signaling by CD45 segregation at 'close contacts'

(2016) Veronica T Chang et al.   NATURE IMMUNOLOGY

Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains

(2015) Katharina Beck-García et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH

FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting

(2015) Dominic Waithe et al.   BIOINFORMATICS

Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins

(2015) Riya Raghupathy et al.   CELL

The T cell receptor resides in ordered plasma membrane nanodomains that aggregate upon patching of the receptor

(2015) Jelena Dinic et al.   Scientific Reports

Effect of hydrophobic mismatch on domain formation and peptide sorting in the multicomponent lipid bilayers in the presence of immobilized peptides

(2014) Qing Liang et al.   JOURNAL OF CHEMICAL PHYSICS

Resolving Internal Motional Correlations to Complete the Conformational Entropy Meter

(2014) Iztok Urbančič et al.   Journal of Physical Chemistry Letters

Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells

(2014) Alf Honigmann et al.   Nature Communications

A lipid bound actin meshwork organizes liquid phase separation in model membranes

(2014) Alf Honigmann et al.   eLife

Direct Detection of S-Palmitoylation by Mass Spectrometry

(2013) Yuhuan Ji et al.   ANALYTICAL CHEMISTRY

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

(2013) Gregory J. Hardy et al.   CURRENT OPINION IN COLLOID & INTERFACE SCIENCE

Bile Acids Modulate Signaling by Functional Perturbation of Plasma Membrane Domains

(2013) Yong Zhou et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bleaching-corrected fluorescence microspectroscopy with nanometer peak position resolution

(2013) Iztok Urbančič et al.   OPTICS EXPRESS

Reduced Lateral Mobility of Lipids and Proteins in Crowded Membranes

(2013) Joseph E. Goose et al.   PLoS Computational Biology

Actin filaments attachment at the plasma membrane in live cells cause the formation of ordered lipid domains

(2012) Jelena Dinic et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

Cholesterol and Sphingomyelin Drive Ligand-independent T-cell Antigen Receptor Nanoclustering

(2012) Eszter Molnár et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Conformational states of the kinase Lck regulate clustering in early T cell signaling

(2012) Jérémie Rossy et al.   NATURE IMMUNOLOGY

Elucidating membrane structure and protein behavior using giant plasma membrane vesicles

(2012) Erdinc Sezgin et al.   Nature Protocols

Switchable Nile Red-Based Probe for Cholesterol and Lipid Order at the Outer Leaflet of Biomembranes

(2010) Oleksandr A. Kucherak et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

T-Cell Receptor Microclusters Critical for T-Cell Activation Are Formed Independently of Lipid Raft Clustering

(2010) A. Hashimoto-Tane et al.   MOLECULAR AND CELLULAR BIOLOGY

High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells

(2010) Dylan M. Owen et al.   MOLECULAR MEMBRANE BIOLOGY

Revitalizing membrane rafts: new tools and insights

(2010) Kai Simons et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Palmitoylation regulates raft affinity for the majority of integral raft proteins

(2010) I. Levental et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

TCR and Lat are expressed on separate protein islands on T cell membranes and concatenate during activation

(2009) Björn F Lillemeier et al.   NATURE IMMUNOLOGY

Order of lipid phases in model and plasma membranes

(2009) H.-J. Kaiser et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Regulation of T Cell Receptor Activation by Dynamic Membrane Binding of the CD3ɛ Cytoplasmic Tyrosine-Based Motif

(2008) Chenqi Xu et al.   CELL

Functional Implications of Plasma Membrane Condensation for T Cell Activation

(2008) Carles Rentero et al.   PLoS One

The molecular face of lipid rafts in model membranes

(2008) H. J. Risselada et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein Shape and Crowding Drive Domain Formation and Curvature in Biological Membranes

(2007) Raoul N. Frese et al.   BIOPHYSICAL JOURNAL