Article
Immunology
Mateusz Maciejczyk, Milosz Nesterowicz, Julita Szulimowska, Anna Zalewska
Summary: This study demonstrates that modifications of salivary proteins increase with age, along with oxidative modifications of lipids and nucleic acids. Salivary redox biomarkers poorly correlate with plasma levels, but can reflect blood concentrations of AGE and 8-OHdG. Salivary AGE and 8-OHdG are valuable diagnostic tools for assessing the aging process.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Eduardo Fuentes-Lemus, Michael J. Davies
Summary: Biological milieus are complex and crucial for metabolic regulation. Understanding the effects of crowded and heterogeneous systems on biochemical processes is important. This review discusses the impact of crowding, nanodomain formation, and altered viscosities on proteins, as well as the modulation of reactions and pathways involving proteins and oxidants. Knowledge of protein modification processes under conditions mimicking biological milieus can provide insights into cellular responses to stressors and their role in aging, signaling, health, and disease.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Yekatarina Gadasheva, Alexander Nolze, Claudia Grossmann
Summary: Cardiovascular aging leads to increased arterial stiffness and left ventricular hypertrophy, with the renin-angiotensin-aldosterone system playing a key role in regulating electrolyte balance and blood pressure. The mineralocorticoid receptor can undergo enzymatic and non-enzymatic modifications, impacting its activity independently of its ligand aldosterone. These modifications may have implications for cardiovascular aging and diseases.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Nutrition & Dietetics
Arleta Dolowacka-Jozwiak, Adam Matkowski, Izabela Nawrot-Hadzik
Summary: Compounds extracted from the rhizomes of Japanese, Giant, and Bohemian knotweeds show promising antiglycoxidative potential for the prevention and complementary treatment of diabetes mellitus, with some fractions exhibiting comparable or stronger activity than the reference drug aminoguanidine.
Article
Biochemistry & Molecular Biology
David Seynnaeve, Daniel P. Mulvihill, Joris Winderickx, Vanessa Franssens
Summary: Our study demonstrates that the lack of Glo2 and Gre3 activity leads to increased formation of large Synphilin-1 inclusions in S. cerevisiae, correlating with enhanced oxidative stress levels and inhibitory effects on growth. Similarly, polar-localised inclusions were observed in S. pombe, but no growth defects were observed upon expression of SNCAIP. Overall, yeasts, especially S. cerevisiae, serve as valuable models for understanding the mechanisms underlying Synphilin-1 pathology in neurodegenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Zongshuai Zhu, Anthony Pius Bassey, Iftikhar Ali Khan, Ming Huang, Xibin Zhang
Summary: The study demonstrates that catechins can inhibit the generation of AGEs in muscle protein through various mechanisms, including influencing lysine and free amino group, alleviating the Maillard reaction, detaching the particle size, affecting the protein-catechins interaction, relieving aggregates, and scavenging free radicals.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Suze Ma, Heng Chen, He Li, Xinjian Ji, Zixin Deng, Wei Ding, Qi Zhang
Summary: The study reveals a enzyme named three-residue cyclophane forming enzyme (3-CyFE) exhibiting remarkable catalytic promiscuity, producing cyclophane and aminomalonate. Bioinformatic analysis indicates that 3-CyFEs have evolved from a common ancestor with anaerobic sulfatase maturases (anSMEs) and share a similar set of catalytic residues.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Microbiology
Adrianna Lupkowska, Soroosh Monem, Janusz Debski, Karolina Stojowska-Swedrzynka, Dorota Kuczynska-Wisnik, Ewa Laskowska
Summary: Research has revealed that protein aggregates are formed during desiccation and rehydration in Escherichia coli, and these aggregates mainly consist of proteins prone to liquid-liquid phase separation. Carnosine, glycine betaine, and trehalose can reduce protein aggregation and glycation, enhancing the survival of E. coli. Interestingly, while high concentrations of glycine betaine and trehalose promote protein aggregation, glycation is still inhibited, and E. coli cells have better survival rates during desiccation-rehydration stress.
MICROBIOLOGICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Ivana Sirangelo, Clara Iannuzzi
Summary: The study provides an overview of the molecular effects induced by glycation on the amyloid aggregation process of protein models associated with misfolding diseases. Glycation plays a key role in protein folding, kinetics of amyloid formation, and amyloid cytotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Gemma Serra-Bardenys, Sandra Peiro
Summary: Oxidoreductases catalyze oxidation-reduction reactions and constitute a large and diverse group of enzymes. Carbonylation, particularly in lysine residues, is a significant oxidative modification in proteins that can impact protein structure and function.
Article
Medicine, Research & Experimental
Krzysztof Drygalski, Eliza Fereniec, Anna Zalewska, Adam Kretowski, Malgorzata Zendzian-Piotrowska, Mateusz Maciejczyk
Summary: The study found that phloroglucinol has strong and broad anti-glycation and anti-oxidative effects, making it a potential adjunct therapy for diabetes.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Chemistry, Medicinal
Marko Bunc, San Hadzi, Christian Graf, Matjaz Boncina, Jurij Lah
Summary: Scientists have developed a platform based on temperature-dependent aggregation data analysis, which can shorten the assessment of antibody stability and accelerate the optimization of antibody formulations. The strategy has shown accurate prediction of aggregate fractions for different therapeutic antibodies and has proved to be effective in the development and production of biological therapeutics.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Md Yousof Ali, Susoma Jannat, M. Mizanur Rahman
Summary: The study found that ginsenosides, particularly Rh2, Rh1, and compound K, demonstrate strong potential in inhibiting the formation of advanced glycation end-products (AGEs), suggesting they may be promising agents for preventing diabetic complications.
BIOORGANIC CHEMISTRY
(2021)
Article
Neurosciences
Engie Prifti, Eleni N. Tsakiri, Ergina Vourkou, George Stamatakis, Martina Samiotaki, Efthimios M. C. Skoulakis, Katerina Papanikolopoulou
Summary: Tau accumulation is associated with Alzheimer's disease and other Tauopathies. This study identifies Mical as a novel protein that interacts with human Tau and alters its interactions with microtubules and the Actin cytoskeleton. Mical's redox activity, specifically the oxidation of Cys322, is shown to play a crucial role in Tau aggregation propensity and toxicity.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Shiva Izadi, Ulrike Vavra, Stanislav Melnik, Clemens Gruenwald-Gruber, Esther Foederl-Hoebenreich, Markus Sack, Kurt Zatloukal, Josef Gloessl, Eva Stoeger, Lukas Mach, Alexandra Castilho, Richard Strasser
Summary: Researchers engineered ACE2-Fc by modifying its glycan structure and obtained an ACE2-Fc variant with enhanced virus-neutralization activity, which shows promise as a potential drug candidate against coronavirus infections.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Xingjun Fan, Sheng Zhou, Benlian Wang, Grant Hom, Minfei Guo, Binbin Li, Jing Yang, Dennis Vaysburg, Vincent M. Monnier
MOLECULAR & CELLULAR PROTEOMICS
(2015)
Article
Biochemistry & Molecular Biology
Zongbo Wei, Caili Hao, Jingru Huangfu, Ramkumar Srinivasagan, Xiang Zhang, Xingjun Fan
Summary: This study reveals that lens epithelial cells are highly susceptible to ferroptosis, and aged and cataractous human lenses may possess more pro-ferroptotic criteria than any other organ in the human body.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Cell Biology
Jingru Huangfu, Caili Hao, Zongbo Wei, I. Michael Wormstone, Hong Yan, Xingjun Fan
Summary: The study reveals that cFLIP is a critical gene regulating lens epithelial cell survival, with an important impact on the response to TNF alpha stimulation.
CELL DEATH & DISEASE
(2021)
Article
Ophthalmology
Zongbo Wei, Caili Hao, Jian-Kang Chen, Lin Gan, Xingjun Fan
Summary: In this study, a lens-specific inducible Cre mouse model was established for gene targeting research in a specific spatiotemporal manner. The results showed that Cre recombinase leaked mainly in the lens cortex and nucleus, while no leakage was observed in the lens epithelium. Furthermore, administration of tamoxifen effectively suppressed target gene expression in both lens epithelium and cortex. The findings of this study are of great significance for the research of lens aging, posterior capsule opacification, and other areas requiring precision gene targeting.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Pathology
Zongbo Wei, Jane Caty, Jeremy Whitson, Amy D. Zhang, Ramkumar Srinivasagan, Terrance J. Kavanagh, Hong Yan, Xingjun Fan
AMERICAN JOURNAL OF PATHOLOGY
(2017)
Article
Ophthalmology
He-Yan Li, Li Dong, Xu-Han Shi, Rui-Heng Zhang, Wen-Da Zhou, Hao-Tian Wu, Chu-Yao Yu, Yi-Tong Li, Yi-Fan Li, Jost B. Jonas, Wen-Bin Wei, Yue-Ming Liu
Summary: This study aimed to examine the intraocular tolerability of cetuximab when applied intravitreally, and its effect on axial elongation. The results showed that intravitreal injections of cetuximab could reduce axial elongation in a dose-dependent and number of treatment-dependent manner, without causing intraocular toxic effects.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
James D. Cole, John A. McDaniel, Joelle Nilak, Ashley Ban, Carlos Rodriguez, Zuhaad Hameed, Marta Grannonico, Peter A. Netland, Hu Yang, Ignacio Provencio, Xiaorong Liu
Summary: Aniridia is a panocular condition characterized by the loss of iris, caused mainly by mutations in the PAX6 gene. This study assessed the effects of Pax6-haploinsufficiency on retinal morphology and vision in Pax6Sey mice. The results showed elevated intraocular pressure and declining visual acuity in Pax6Sey mice, along with local retinal damage and a possible neuroinflammatory response.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Subao Jiang, Ning Xia, Francesco Buonfiglio, Elsa W. Boehm, Qi Tang, Norbert Pfeiffer, Dominik Olinger, Huige Li, Adrian Gericke
Summary: This study found that a high-fat diet induces endothelial dysfunction in the ophthalmic artery, leading to impaired vascular function in the eye. The high-fat diet triggers oxidative stress and involves RAGE and NOX2.
EXPERIMENTAL EYE RESEARCH
(2024)
Review
Ophthalmology
Mala Upadhyay, Vera L. Bonilha
Summary: The sodium iodate (NaIO3) model is a valuable tool in studying dry AMD and evaluating the retinal structure. Through extensive research, it has been found that the death of RPE cells caused by NaIO3 injection is closely related to vision loss in patients with dry AMD. Therefore, studying NaIO3 can provide insights into the mechanisms of RPE and photoreceptor cell death and contribute to understanding RPE degeneration in AMD.
EXPERIMENTAL EYE RESEARCH
(2024)
Article
Ophthalmology
Vicki Chrysostomou, Katharina C. Bell, Sze Woei Ng, Samyuktha Suresh, Gayathri Karthik, Marion Millet, Yingying Chung, Jonathan G. Crowston
Summary: We present a new experimental model for inducing retinal ganglion cell (RGC) dysfunction and degeneration in mice. This model allows for the reliable induction of RGC dysfunction and loss, with the resilience of the optic nerve being dependent on the age of the animal. The time-defined nature of the functional and structural changes observed in this model will facilitate the study of glial responses and the evaluation of neuroprotective treatments after intraocular pressure (IOP) injury.
EXPERIMENTAL EYE RESEARCH
(2024)