Article
Immunology
Siyang Yu, Peiyan Zhang, Mingfeng Liao, Juanjuan Zhang, Suisui Luo, Jinglei Zhai, Yaxi Zhang, Jingyan Lin, Jing Yuan, Zheng Zhang, Fuxiang Wang, Lanlan Wei
Summary: This study found that saliva IgA could serve as a useful index for early diagnosis of COVID-19. Through testing saliva and serum specimens from 44 COVID-19 patients, the results showed higher COI values for saliva IgA and lower levels of IgG in saliva samples compared to serum samples.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
A. M. M. Murillo, J. Tome-Amat, Y. Ramirez, M. Garrido-Arandia, L. G. Valle, G. Hernandez-Ramirez, L. Tramarin, P. Herreros, B. Santamaria, A. Diaz-Perales, M. Holgado
Summary: The paper presents a rapid, accurate, and cost-effective method for detecting antibodies against SARS-CoV-2, which can be used periodically for early detection, surveillance, immunity detection, and control of COVID-19 spread. The results obtained using this method were compared with those obtained using the traditional ELISA method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Immunology
Ryan M. Pace, Janet E. Williams, Kirsi M. Jarvinen, Courtney L. Meehan, Melanie A. Martin, Sylvia H. Ley, Celestina Barbosa-Leiker, Aline Andres, Laxmi Yeruva, Mandy B. Belfort, Beatrice Caffe, Alexandra D. Navarrete, Kimberly A. Lackey, Christina D. W. Pace, Alexandra C. Gogel, Bethaney D. Fehrenkamp, Miranda Klein, Bridget E. Young, Casey Rosen-Carole, Nichole Diaz, Stephanie L. Gaw, Valerie Flaherman, Mark A. McGuire, Michelle K. McGuire, Antti E. Seppo
Summary: The study found that milk produced by women with COVID-19 does not contain SARS-CoV-2 and contains anti-RBD IgA, providing lasting passive immunity. Therefore, it is recommended for lactating women to continue breastfeeding during and after COVID-19 infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Pandikannan Krishnamoorthy, Athira S. Raj, Himanshu Kumar
Summary: The COVID-19 pandemic caused by SARS-CoV-2 continues to be a global health threat. Early diagnosis is challenging due to overlapping symptoms with other respiratory infections. This study implemented bioinformatics and machine learning to identify a 7-gene biomarker that can distinguish SARS-CoV-2-associated respiratory illness and severe COVID-19 patients. The biomarker's dysregulation during severe disease is restored during recovery, indicating its prognostic potential and suggesting it as a potential diagnostic candidate to reduce COVID-19 mortality.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Genetics & Heredity
Marta Selma-Royo, Christine Bauerl, Desiree Mena-Tudela, Laia Aguilar-Camprubi, Francisco J. Perez-Cano, Anna Parra-Llorca, Carles Lerin, Cecilia Martinez-Costa, Maria Carmen Collado
Summary: This study aimed to evaluate the time course of SARS-CoV-2-specific IgA and IgG induction in breast milk after vaccination. The results showed that the presence and persistence of specific SARS-CoV-2 antibodies in breast milk were dependent on the vaccine type and previous virus exposure. The levels of antibodies in breast milk from vaccinated women were lower than in milk from COVID-19-infected women but higher than in milk from uninfected women.
Article
Pediatrics
Dolores Sabina Romero Ramirez, Maria Magdalena Lara Perez, Mercedes Carretero Perez, Maria Isis Suarez Hernandez, Saul Martin Pulido, Lorena Pera Villacampa, Ana Maria Fernandez Vilar, Monica Rivero Falero, Paloma Gonzalez Carretero, Beatriz Reyes Millan, Sabine Roper, Miguel Angel Garcia Bello
Summary: This study found a clear association between COVID-19 vaccination and specific immunoglobulin concentrations in human milk. The effect was more pronounced when lactation periods exceeded 23 months and was specific and independent of other variables.
Article
Immunology
Maurizio Guida, Daniela Terracciano, Michele Cennamo, Federica Aiello, Evelina La Civita, Gennaro Esposito, Valentina Gargiulo, Giuseppe M. Maruotti, Giuseppe Portella, Laura Sarno
Summary: The study demonstrated the presence of anti-SARS-CoV-2 S antibodies in the breast milk of women vaccinated with mRNABNT162b2, suggesting that vaccinating breastfeeding women may be a strategy to protect their infants from COVID-19 infection.
Article
Immunology
Jamil A. Al-Mughales, Tareq J. Al-Mughales, Omar I. Saadah
Summary: The study aimed to monitor specific anti-SARS-CoV-2 IgG and IgM antibody production in severe COVID-19 patients using different commercially available tests. Results showed that IgG seroconversion rates increased with time in all tests, while IgM detection was inconsistent and showed slight differences between the two quantitative assays. There was no significant association between mortality and IgG or IgM seroconversion rates or concentrations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Juanjie Tang, Gabrielle Grubbs, Youri Lee, Hua Wu, Thomas C. Luke, Kristi A. Egland, Christoph L. Bausch, Eddie J. Sullivan, Surender Khurana
Summary: Polyclonal human antibodies derived from hyperimmunized transchromosomic bovines with SARS-CoV-2 spike exhibit broad cross-neutralization activity against emerging variants, indicating their potential for COVID-19 treatment.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Immunology
Luca Ferrari, Chiara Favero, Giulia Solazzo, Jacopo Mariani, Anna Luganini, Monica Ferraroni, Emanuele Montomoli, Gregorio Paolo Milani, Valentina Bollati, UNICORN Consortium
Summary: The composition of the nasopharyngeal microbiota may be associated with the maintenance of antibodies against SARS-CoV-2 infection. Further research is needed to understand how the nasopharyngeal microbiota influences the immune response to viral infections.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Marina Rieder, Luisa Wirth, Luisa Pollmeier, Maren Jeserich, Isabella Goller, Niklas Baldus, Bonaventura Schmid, Hans-Joerg Busch, Maike Hofmann, Robert Thimme, Siegbert Rieg, Winfried Kern, Christoph Bode, Daniel Duerschmied, Achim Lother
Summary: Level of interleukin 6 (IL-6) is associated with disease severity in COVID-19, however, there is also upregulation of immunomodulatory protein progranulin (GRN) which is specifically observed in SARS-CoV-2-positive patients. High GRN abundance is linked to adverse outcomes and increased IL-6 expression in COVID-19.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Maria Denaro, Elisa Ferro, Giuseppe Barrano, Salvatore Meli, Mariangela Busacca, Damiano Corallo, Alessia Capici, Alessandra Zisa, Luana Cucuzza, Sandra Gradante, Marialuisa Occhipinti, Paola Santalucia, Raffaele Elia, Angelo Aliquo, Daniele Tibullo, Carmelo Fidone, Vincenzo Bramanti
Summary: The study found that the trend of SARS-CoV-2 variants in Ragusa area is comparable to the Sicily region based on genomic sequencing and immune monitoring of 600 samples. Although no correlation was found between variants and clinical symptoms, antibody levels were positively correlated with an increase in the number of symptoms. Furthermore, SARS-CoV-2 infection induced a significantly higher enhancement in antibody titers compared to vaccine administration. Therefore, the evaluation of anti-N IgG levels could be used as an early marker to identify asymptomatic individuals in the post-pandemic period.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
S. Momsen Reincke, Meng Yuan, Hans-Christian Kornau, Victor M. Corman, Scott van Hoof, Elisa Sanchez-Sendin, Melanie Ramberger, Wenli Yu, Yuanzi Hua, Henry Tien, Marie Luisa Schmidt, Tatjana Schwarz, Lara Maria Jeworowski, Sarah E. Brandl, Helle Foverskov Rasmussen, Marie A. Homeyer, Laura Stoffler, Martin Barner, Desiree Kunkel, Shufan Huo, Johannes Horler, Niels von Wardenburg, Inge Kroidl, Tabea M. Eser, Andreas Wieser, Christof Geldmacher, Michael Hoelscher, Hannes Ganzer, Gunter Weiss, Dietmar Schmitz, Christian Drosten, Harald Pruss, Ian A. Wilson, Jakob Kreye
Summary: The Beta variant of concern (VOC) of SARS-CoV-2 shows resistance to neutralization by antibodies from COVID-19 patients and vaccinated individuals. Researchers have identified specific antibodies in serum from Beta-infected patients that are able to cross-react with the wild type virus. This finding provides insights into the antibody response to antigenic drift and has implications for the development of future vaccines and therapeutics.
Article
Chemistry, Analytical
Sebastian Cajigas, Daniel Alzate, Maritza Fernandez, Carlos Muskus, Jahir Orozco
Summary: This study reports on an electrochemical genosensor specifically designed for the detection and discrimination of SARS-CoV-2. The genosensor demonstrated high sensitivity and specificity, allowing for the detection of SARS-CoV-2 and differentiation from homologous viruses, providing a promising approach for the diagnosis and surveillance of COVID-19.
Review
Cell Biology
Kid Tornquist, Muhammad Yasir Asghar, Vignesh Srinivasan, Laura Korhonen, Dan Lindholm
Summary: SARS-CoV-2 virus enters cells through binding with ACE2 protein and specific protease priming, while sphingolipids affect the uptake of virus particles. Inhibition of ASM function and S1P levels may be related to disease severity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Eugenia Voytik, Isabell Bludau, Sander Willems, Fynn M. Hansen, Andreas-David Brunner, Maximilian T. Strauss, Matthias Mann
Summary: AlphaMap is a Python package for visual exploration of peptide-level proteomics data, mapping identified peptides and post-translational modifications to protein sequences with prior knowledge from UniProt. It provides an intuitive graphical user interface and flexibility as a Python package for integration into common analysis workflows for data visualization.
Article
Biotechnology & Applied Microbiology
Alberto Santos, Ana R. Colaco, Annelaura B. Nielsen, Lili Niu, Maximilian Strauss, Philipp E. Geyer, Fabian Coscia, Nicolai J. Wewer Albrechtsen, Filip Mundt, Lars Juhl Jensen, Matthias Mann
Summary: A knowledge graph platform integrates proteomics with other omics data and biomedical databases, providing support for precision medicine. The Clinical Knowledge Graph (CKG) is a flexible data model that can accommodate a large number of nodes and relationships, accelerating the analysis and interpretation of proteomics data. By incorporating CKG, clinical decision-making can be informed and proteomics data can be enriched.
NATURE BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Eric Linster, Francy L. Forero Ruiz, Pavlina Miklankova, Thomas Ruppert, Johannes Mueller, Laura Armbruster, Xiaodi Gong, Giovanna Serino, Matthias Mann, Ruediger Hell, Markus Wirtz
Summary: N-terminal protein acetylation is a vital protein modification for the viability of animals and plants. New research reveals that depletion of NatA activity increases global protein turnover and triggers feedback mechanisms to maintain protein abundance. NatA substrates contribute to the majority of this enhanced turnover, and the absence of NTA leads to protein destabilization through a previously undescribed nonAc/N-degron.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Lili Niu, Maja Thiele, Philipp E. Geyer, Ditlev Nytoft Rasmussen, Henry Emanuel Webel, Alberto Santos, Rajat Gupta, Florian Meier, Maximilian Strauss, Maria Kjaergaard, Katrine Lindvig, Suganya Jacobsen, Simon Rasmussen, Torben Hansen, Aleksander Krag, Matthias Mann
Summary: This study used mass spectrometry analysis to investigate the liver and plasma proteomics of patients with alcohol-related liver disease. Noninvasive biomarkers associated with early stages of disease progression were identified. Machine learning models revealed that these biomarkers could accurately detect fibrosis and inflammation, and predict future liver-related events and all-cause mortality.
Article
Biochemistry & Molecular Biology
Isabell Bludau, Sander Willems, Wen-Feng Zeng, Maximilian T. Strauss, Fynn M. Hansen, Maria C. Tanzer, Ozge Karayel, Brenda A. Schulman, Matthias Mann
Summary: The recent revolution in computational protein structure prediction has provided new insights into the study of the entire proteome. In this study, the researchers analyze posttranslational modifications (PTMs) of proteins to determine their structural context and investigate their potential regulatory sites. The analysis reveals global patterns of PTM occurrence and spatial coregulation of different types of PTMs.
Article
Multidisciplinary Sciences
Dari Shalon, Rebecca Neal Culver, Jessica A. Grembi, Jacob Folz, Peter V. Treit, Handuo Shi, Florian A. Rosenberger, Les Dethlefsen, Xiandong Meng, Eitan Yaffe, Andres Aranda-Diaz, Philipp E. Geyer, Johannes B. Mueller-Reif, Sean Spencer, Andrew D. Patterson, George Triadafilopoulos, Susan P. Holmes, Matthias Mann, Oliver Fiehn, David A. Relman, Kerwyn Casey Huang
Summary: This study developed an ingestible device that can collect samples from multiple regions of the human intestinal tract during normal digestion. Multi-omics analysis of the collected samples revealed significant differences in bacteria, phages, host proteins, and metabolites between the intestines and stool. The study provides insights into the roles of the gut microbiome and metabolome in human physiology and disease through non-invasive longitudinal profiling of microorganisms, proteins, and bile acids along the intestinal tract.
Article
Multidisciplinary Sciences
Susanne C. M. Reinhardt, Luciano A. Masullo, Isabelle Baudrexel, Philipp R. Steen, Rafal Kowalewski, Alexandra S. Eklund, Sebastian Strauss, Eduard M. Unterauer, Thomas Schlichthaerle, Maximilian T. Strauss, Christian Klein, Ralf Jungmann
Summary: Fluorescence microscopy is a major characterization method in the life sciences, but current super-resolution techniques do not achieve Angstrom resolution. This study introduces a DNA-barcoding method that greatly improves the resolution of fluorescence microscopy, allowing for single-protein resolution in intact cells. The method also demonstrates Angstrom resolution for DNA origami.
Article
Multidisciplinary Sciences
Manuela Thienel, Johannes B. Mueller-Reif, Zhe Zhang, Vincent Ehreiser, Judith Huth, Khrystyna Shchurovska, Badr Kilani, Lisa Schweizer, Philipp E. Geyer, Maximilian Zwiebel, Julia Novotny, Enzo Luesebrink, Gemma Little, Martin Orban, Leo Nicolai, Shaza El Nemr, Anna Titova, Michael Spannagl, Jonas Kindberg, Alina L. Evans, Orpheus Mach, Matthias Vogel, Steffen Tiedt, Steffen Ormanns, Barbara Kessler, Anne Dueck, Andrea Friebe, Peter Godsk Jorgensen, Monir Majzoub-Altweck, Andreas Blutke, Amin Polzin, Konstantin Stark, Stefan Kaab, Doris Maier, Jonathan M. Gibbins, Ulrich Limper, Ole Frobert, Matthias Mann, Steffen Massberg, Tobias Petzold
Summary: This study identified a mechanism of thromboprotection in immobilized brown bears and spinal cord injury patients, involving the down-regulation of heat shock protein 47 (HSP47) in platelets. This resulted in attenuated immune cell activation and neutrophil extracellular trap formation, providing potential therapeutic targets for antithrombotic treatments.
Article
Biochemistry & Molecular Biology
Sander Wuyts, Renato Alves, Maria Zimmermann-Kogadeeva, Suguru Nishijima, Sonja Blasche, Marja Driessen, Philipp E. Geyer, Rajna Hercog, Ece Kartal, Lisa Maier, Johannes B. Mueller, Sarela Garcia Santamarina, Thomas Sebastian B. Schmidt, Daniel C. Sevin, Anja Telzerow, Peter Treit, Tobias Wenzel, Athanasios Typas, Kiran R. Patil, Matthias Mann, Michael Kuhn, Peer Bork
Summary: Multi-omics analyses were used to study the molecular changes in microbial communities under different conditions. Using five omics layers, the study mapped the molecular response of a synthetic community of gut bacteria to three non-antibiotic drugs. The results showed that the omics methods were consistent in estimating relative species abundances and complemented each other in capturing functional changes.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Biochemical Research Methods
Florian A. Rosenberger, Marvin Thielert, Maximilian T. Strauss, Lisa Schweizer, Constantin Ammar, Sophia C. Maedler, Andreas Metousis, Patricia Skowronek, Maria Wahle, Katherine Madden, Janine Gote-Schniering, Anna Semenova, Herbert B. Schiller, Edwin Rodriguez, Thierry M. Nordmann, Andreas Mund, Matthias Mann
Summary: Single-cell proteomics by mass spectrometry is a powerful method for studying biological heterogeneity, and single-cell Deep Visual Proteomics (scDVP) is a technology that integrates high-content imaging, laser microdissection, and multiplexed mass spectrometry to analyze the spatial proteome of tissues. It can identify thousands of proteins and reveal differential regulation in spatially distinct regions.
Article
Biochemical Research Methods
Elena Krismer, Isabell Bludau, Maximilian T. Strauss, Matthias Mann
Summary: AlphaPeptStats is a comprehensive Python package for normalization, imputation, visualization, and statistical analysis of label-free proteomics data. It provides a wide range of statistical algorithms and data visualization capabilities, allowing researchers to manually or automatically explore complex datasets and identify interesting patterns and outliers.
Meeting Abstract
Biochemical Research Methods
Andre C. Michaelis, Andreas-David Brunner, Maximilian Zwiebel, Florian Meier, Maximilian T. Strauss, Isabell Bludau, Matthias Mann
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Meeting Abstract
Gastroenterology & Hepatology
Lili Niu, Maja Thiele, Philipp Geyer, Ditlev Nytoft Rasmussen, Henry Emanuel Webel, Alberto Delgado, Rajat Gupta, Florian Meier, Maximilian Strauss, Maria Kjaergaard, Katrine Prier Lindvig, Mads Israelsen, Simon Rasmussen, Torben Hansen, Matthias Mann, Aleksander Krag
JOURNAL OF HEPATOLOGY
(2022)