Glycan utilization systems in the human gut microbiota: a gold mine for structural discoveries

Crystal structure of β-L-arabinobiosidase belonging to glycoside hydrolase family 121

(2020) Keita Saito et al.   PLoS One

A pair of esterases from a commensal gut bacterium remove acetylations from all positions on complex β-mannans

(2020) Leszek Michalak et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Synergy between Cell Surface Glycosidases and Glycan-Binding Proteins Dictates the Utilization of Specific Beta(1,3)-Glucans by Human Gut Bacteroides

(2020) Guillaume Déjean et al.   mBio

An Epoxide Intermediate in Glycosidase Catalysis

(2020) Lukasz F. Sobala et al.   ACS Central Science

Structural and functional insights into oligopeptide acquisition by the RagAB transporter from Porphyromonas gingivalis

(2020) Mariusz Madej et al.   Nature Microbiology

Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown

(2020) Lucy I. Crouch et al.   Nature Communications

Butyrate producing colonic Clostridiales metabolise human milk oligosaccharides and cross feed on mucin via conserved pathways

(2020) Michael Jakob Pichler et al.   Nature Communications

A Cell-Surface GH9 Endo-Glucanase Coordinates with Surface Glycan Binding Proteins to Mediate Xyloglucan Uptake in the Gut Symbiont Bacteroides ovatus

(2019) Matthew H. Foley et al.   JOURNAL OF MOLECULAR BIOLOGY

The human gut Firmicute Roseburia intestinalis is a primary degrader of dietary β-mannans

(2019) Sabina Leanti La Rosa et al.   Nature Communications

Transforming medicine with the microbiome

(2019) Niv Zmora et al.   Science Translational Medicine

Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space

(2019) William Helbert et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Structural and functional analysis of glycoside hydrolase 138 enzymes targeting chain A galacturonic acid in the complex pectin rhamnogalacturonan II

(2019) Aurore Labourel et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Two binding proteins of the ABC transporter that confers growth of Bifidobacterium animalis subsp. lactis ATCC27673 on β‐mannan possess distinct manno‐oligosaccharide‐binding profiles

(2019) M. Ejby et al.   MOLECULAR MICROBIOLOGY

The Integrative Human Microbiome Project

(2019)   NATURE

Surface glycan-binding proteins are essential for cereal beta-glucan utilization by the human gut symbiont Bacteroides ovatus

(2019) Kazune Tamura et al.   CELLULAR AND MOLECULAR LIFE SCIENCES

Substrate preference of an ABC importer corresponds to selective growth on β-(1,6)-galactosides in Bifidobacterium animalis subsp. lactis

(2019) Mia Christine Theilmann et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Bacteroidetes use thousands of enzyme combinations to break down glycans

(2019) Pascal Lapébie et al.   Nature Communications

Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci

(2019) Justina Briliūtė et al.   Nature Microbiology

Diet–microbiota interactions and personalized nutrition

(2019) Aleksandra A. Kolodziejczyk et al.   NATURE REVIEWS MICROBIOLOGY

Evolutionary adaptation in fucosyllactose uptake systems supports bifidobacteria-infant symbiosis

(2019) Mikiyasu Sakanaka et al.   Science Advances

Molecular insight into a new low‐affinity xylan binding module from the xylanolytic gut symbiont Roseburia intestinalis

(2019) Maria Louise Leth et al.   FEBS Journal

A subfamily roadmap of the evolutionarily diverse glycoside hydrolase family 16 (GH16)

(2019) Alexander Holm Viborg et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Starch Digestion by Gut Bacteria: Crowdsourcing for Carbs

(2019) Filipe M. Cerqueira et al.   TRENDS IN MICROBIOLOGY

The complex of ferric-enterobactin with its transporter from Pseudomonas aeruginosa suggests a two-site model

(2019) Lucile Moynié et al.   Nature Communications

The Starch Utilization System Assembles around Stationary Starch-Binding Proteins

(2018) Hannah H. Tuson et al.   BIOPHYSICAL JOURNAL

TonB-dependent transport by the gut microbiota: novel aspects of an old problem

(2018) David N Bolam et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Glycan Utilization and Cross-Feeding Activities by Bifidobacteria

(2018) Francesca Turroni et al.   TRENDS IN MICROBIOLOGY

Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont

(2018) Benjamin Pluvinage et al.   Nature Communications

Differential bacterial capture and transport preferences facilitate co-growth on dietary xylan in the human gut

(2018) Maria Louise Leth et al.   Nature Microbiology

Functional categorisation of dietary fibre in foods: Beyond ‘soluble’ vs ‘insoluble’

(2018) Michael J. Gidley et al.   TRENDS IN FOOD SCIENCE & TECHNOLOGY

Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes

(2017)   GLYCOBIOLOGY

Polysaccharide Utilization Loci: Fueling Microbial Communities

(2017) Julie M. Grondin et al.   JOURNAL OF BACTERIOLOGY

The first crystal structure of a family 129 glycoside hydrolase from a probiotic bacterium reveals critical residues and metal cofactors

(2017) Mayo Sato et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

An evolutionarily distinct family of polysaccharide lyases removes rhamnose capping of complex arabinogalactan proteins

(2017) José Munoz-Munoz et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Biochemical and Structural Analyses of Two Cryptic Esterases in Bacteroides intestinalis and their Synergistic Activities with Cognate Xylanases

(2017) Daniel Wefers et al.   JOURNAL OF MOLECULAR BIOLOGY

Novel carbohydrate binding modules in the surface anchored α-amylase of Eubacterium rectale provide a molecular rationale for the range of starches used by this organism in the human gut

(2017) Darrell W. Cockburn et al.   MOLECULAR MICROBIOLOGY

Structural basis for nutrient acquisition by dominant members of the human gut microbiota

(2017) Amy J. Glenwright et al.   NATURE

Complex pectin metabolism by gut bacteria reveals novel catalytic functions

(2017) Didier Ndeh et al.   NATURE

How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans

(2017) Alan Cartmell et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Unusual active site location and catalytic apparatus in a glycoside hydrolase family

(2017) Jose Munoz-Munoz et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Recognition of protein-linked glycans as a determinant of peptidase activity

(2017) Ilit Noach et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Reciprocal Prioritization to Dietary Glycans by Gut Bacteria in a Competitive Environment Promotes Stable Coexistence

(2017) Yunus E. Tuncil et al.   mBio

Molecular Mechanism by which Prominent Human Gut Bacteroidetes Utilize Mixed-Linkage Beta-Glucans, Major Health-Promoting Cereal Polysaccharides

(2017) Kazune Tamura et al.   Cell Reports

Dietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides

(2017) Ana S. Luis et al.   Nature Microbiology

A Dietary Fiber-Deprived Gut Microbiota Degrades the Colonic Mucus Barrier and Enhances Pathogen Susceptibility

(2016) Mahesh S. Desai et al.   CELL

Recent structural insights into the enzymology of the ubiquitous plant cell wall glycan xyloglucan

(2016) Mohamed A Attia et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

An updated structural classification of substrate-binding proteins

(2016) Giel H. Scheepers et al.   FEBS LETTERS

An ATP Binding Cassette Transporter Mediates the Uptake of α-(1,6)-Linked Dietary Oligosaccharides inBifidobacteriumand Correlates with Competitive Growth on These Substrates

(2016) Morten Ejby et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular Dissection of Xyloglucan Recognition in a Prominent Human Gut Symbiont

(2016) Alexandra S. Tauzin et al.   mBio

Matching the Diversity of Sulfated Biomolecules: Creation of a Classification Database for Sulfatases Reflecting Their Substrate Specificity

(2016) Tristan Barbeyron et al.   PLoS One

The substrate-binding protein in bacterial ABC transporters: dissecting roles in the evolution of substrate specificity

(2015) A. Maqbool et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Glycan complexity dictates microbial resource allocation in the large intestine

(2015) Artur Rogowski et al.   Nature Communications

Crystal structure of glycoside hydrolase family 127 β-l-arabinofuranosidase from Bifidobacterium longum

(2014) Tasuku Ito et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Crystal structures of apparent saccharide sensors from histidine kinase receptors prevalent in a human gut symbiont

(2014) Zhen Zhang et al.   FEBS Journal

The Devil Lies in the Details: How Variations in Polysaccharide Fine-Structure Impact the Physiology and Evolution of Gut Microbes

(2014) Eric C. Martens et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular details of a starch utilization pathway in the human gut symbiontEubacterium rectale

(2014) Darrell W. Cockburn et al.   MOLECULAR MICROBIOLOGY

A discrete genetic locus confers xyloglucan metabolism in select human gut Bacteroidetes

(2014) Johan Larsbrink et al.   NATURE

Multifunctional Nutrient-Binding Proteins Adapt Human Symbiotic Bacteria for Glycan Competition in the Gut by Separately Promoting Enhanced Sensing and Catalysis

(2014) E. A. Cameron et al.   mBio

Super-Resolution Imaging of Live Anaerobic Bacteria: Assembly and Dynamics of the B. Theta Starch Utilization System

(2013) Krishanthi Karunatilaka et al.   BIOPHYSICAL JOURNAL

Multiple rewards from a treasure trove of novel glycoside hydrolase and polysaccharide lyase structures: new folds, mechanistic details, and evolutionary relationships

(2013) Shinya Fushinobu et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Structural basis for arabinoxylo-oligosaccharide capture by the probioticBifidobacterium animalissubsp.lactis Bl-04

(2013) Morten Ejby et al.   MOLECULAR MICROBIOLOGY

Diet rapidly and reproducibly alters the human gut microbiome

(2013) Lawrence A. David et al.   NATURE

The abundance and variety of carbohydrate-active enzymes in the human gut microbiota

(2013) Abdessamad El Kaoutari et al.   NATURE REVIEWS MICROBIOLOGY

The carbohydrate-active enzymes database (CAZy) in 2013

(2013) Vincent Lombard et al.   NUCLEIC ACIDS RESEARCH

Glycan recognition by the Bacteroidetes Sus-like systems

(2012) David N Bolam et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Multidomain Carbohydrate-binding Proteins Involved inBacteroides thetaiotaomicronStarch Metabolism

(2012) Elizabeth A. Cameron et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

α-N-Acetylgalactosaminidase from Infant-associated Bifidobacteria Belonging to Novel Glycoside Hydrolase Family 129 Is Implicated in Alternative Mucin Degradation Pathway

(2011) Masashi Kiyohara et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Recognition and Degradation of Plant Cell Wall Polysaccharides by Two Human Gut Symbionts

(2011) Eric C. Martens et al.   PLOS BIOLOGY

Molecular Cloning and Characterization of a β-l-Arabinobiosidase inBifidobacterium longumThat Belongs to a Novel Glycoside Hydrolase Family

(2010) Kiyotaka Fujita et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Structure of a SusD Homologue, BT1043, Involved in MucinO-Glycan Utilization in a Prominent Human Gut Symbiont†,‡

(2009) Nicole Koropatkin et al.   BIOCHEMISTRY

Crystallographic and Mutational Analyses of Substrate Recognition of Endo-α-N-acetylgalactosaminidase from Bifidobacterium longum

(2009) Ryuichiro Suzuki et al.   JOURNAL OF BIOCHEMISTRY

Structural and Thermodynamic Analyses of Solute-binding Protein fromBifidobacterium longumSpecific for Core 1 Disaccharide and Lacto-N-biose I

(2008) Ryuichiro Suzuki et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Starch Catabolism by a Prominent Human Gut Symbiont Is Directed by the Recognition of Amylose Helices

(2008) Nicole M. Koropatkin et al.   STRUCTURE