Article
Pharmacology & Pharmacy
He-Chin Hsieh, Chung-Chu Chen, Pin-Han Chou, Wen-Chun Liu, Suh-Chin Wu
Summary: This preclinical study suggests that the RBD-LTA fusion protein could be a promising candidate as a mucosal booster COVID-19 vaccine, eliciting neutralizing antibodies against multiple SARS-CoV-2 variants and IgA antibodies in bronchoalveolar lavage fluids.
ANTIVIRAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Andrea Hlousek-Kasun, Petra Mikolcevic, Johannes Gregor Matthias Rack, Callum Tromans-Coia, Marion Schuller, Gytis Jankevicius, Marija Matkovic, Branimir Bertosa, Ivan Ahel, Andreja Mikoc
Summary: ADP-ribosylation is an important covalent modification that plays critical roles in various biological processes. The toxin/antitoxin pair DarT/DarG has been discovered, where DarT modifies DNA and DarG reverses this modification. The homologue SCO6735 is highly conserved and associated with antibiotic production in bacteria, and it can also reverse the ADP-ribosylation catalyzed by DarT.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Biology
Kelsie M. Rodriguez, Sara C. Buch-Larsen, Ilsa T. Kirby, Ivan Rodriguez Siordia, David Hutin, Marit Rasmussen, Denis M. Grant, Larry L. David, Jason Matthews, Michael L. Nielsen, Michael S. Cohen
Summary: PARP-7 has been identified as an important regulator of the innate immune response, with a key target being the inactive PARP family member PARP-13. Specifically, PARP-7 preferentially MARylates cysteine residues in the RNA binding zinc finger domain of PARP-13, as revealed by proteome-wide ADP-ribosylation analysis.
Review
Immunology
Huifang Zhu, Yan-Dong Tang, Guoqing Zhan, Chenhe Su, Chunfu Zheng
Summary: PARPs, a family of proteins responsible for transferring ADP-ribose groups, play important roles in regulating various cellular functions and have widespread involvement in mammalian innate immunity. Understanding the roles of PARPs and ADP-ribosylation in regulating innate immune responses can help in discovering and designing new molecular targets for cancers, pathogenic infections, and inflammations.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Maria Manuela Rosado, Claudio Pioli
Summary: ADP-ribosylation is a post-translational modification involving the addition of one or more ADP-ribose moieties to acceptor proteins. This process is catalyzed by two major families of enzymes, ARTCs and ARTDs, which play roles in fundamental biological processes such as DNA repair, inflammation, cell death, and immune cell functions. ADP-ribosylation is a dynamic process that regulates the expression of immune mediators and provides plasticity for fast adaptation in immune system functions.
Article
Immunology
Tida Lee, Ramiro L. Gutierrez, Milton Maciel, Steven Poole, Kayla J. Testa, Stefanie Trop, Christopher Duplessis, Alison Lane, Mark S. Riddle, Melinda Hamer, Ashley Alcala, Michael Prouty, Nicole Maier, Rahsan Erdem, A. Louis Bourgeois, Chad K. Porter
Summary: The study evaluated the safety and immunogenicity of the CS6-targeted candidate vaccine CssBA co-administered intramuscularly with the double-mutant heat-labile enterotoxin dmLT. The vaccine was found to be safe and well-tolerated across all dose cohorts, inducing robust antibody responses in a dose-dependent manner with a clear dmLT adjuvant effect. This paves the way for future studies to evaluate the efficacy of this vaccine target and development of a multivalent, subunit ETEC vaccine.
Article
Veterinary Sciences
Qiangde Duan, Shengmei Pang, Lili Feng, Jiaqi Liu, Linfen Lv, Baoliang Li, Yuxuan Liang, Guoqiang Zhu
Summary: Heat-labile enterotoxin enhances bacterial adherence by upregulating adhesin and enterotoxin expression in enterotoxigenic Escherichia coli. These findings provide insights into the pathogenic mechanism of heat-labile enterotoxin and enterotoxigenic Escherichia coli.
VETERINARY RESEARCH
(2022)
Article
Biochemical Research Methods
Stephanie C. Luthi, Anna Howald, Kathrin Nowak, Robert Graage, Giody Bartolomei, Christine Neupert, Xaver Sidler, Deena Leslie Pedrioli, Michael O. Hottiger
Summary: Plasma phosphodiesterases and/or ADP-ribosylhydrolases were found to rapidly remove post-translational modifications in vitro ADP-ribosylated proteins. Mass spectrometry identified a total of 60 ADP-ribosylated proteins, with 17 of them being ADP-ribosylated plasma proteins. This new protocol marks an important advancement in the field of ADP-ribosylation and provides insights into ADP-ribosylomes in blood and plasma under different conditions.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Cell Biology
Yujie Gan, Huanhuan Sha, Renrui Zou, Miao Xu, Yuan Zhang, Jifeng Feng, Jianzhong Wu
Summary: This review provides an update on methodological advances in the study of ADP-ribosylation and discusses the function and therapeutic potential of mono-ADP-ribosylation in cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Marie Eggers, Felix Ruehl, Friedrich Haag, Friedrich Koch-Nolte
Summary: Nanobodies, the single variable immunoglobulin domains of heavy chain antibodies naturally occurring in camelids, have a hydrophilic surface area which makes them highly soluble, robust in harsh environments, and exceptionally easy to format into bispecific reagents. The unique properties of nanobodies have been utilized in modulating purinergic signaling, with examples targeting P2X7, CD38, and ARTC2.2.
BIOCHEMICAL PHARMACOLOGY
(2021)
Article
Microbiology
Miki Okuno, Nami Tsuru, Shuji Yoshino, Yasuhiro Gotoh, Takeshi Yamamoto, Tetsuya Hayashi, Yoshitoshi Ogura
Summary: This study reports the first isolation of an Escherichia fergusonii strain carrying the elt1 gene, which encodes heat-labile enterotoxin 1, from a patient in Japan. Comparative genomics and phylogenetic analyses revealed the presence of elt1-positive E. fergusonii strains isolated from poultry in the United Kingdom. Interestingly, the elt1 gene in the human isolate was integrated into the chromosome, while in the poultry isolate it was present on a large plasmid.
MICROBIOLOGY SPECTRUM
(2023)
Article
Multidisciplinary Sciences
Tomoko Kubori, Junyup Lee, Hyunmin Kim, Kohei Yamazaki, Masanari Nishikawa, Tomoe Kitao, Byung-Ha Oh, Hiroki Nagai
Summary: The intracellular bacterial pathogen Legionella pneumophila utilizes a pair of effector proteins, Lpg0080 and Lpg0081, to coordinately manipulate mitochondrial activity in host cells by enzymatically modifying ADP/ATP translocases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Qiangde Duan, Shengmei Pang, Lili Feng, Baoliang Li, Linfen Lv, Yuxuan Liang, Guoqiang Zhu
Summary: Increasing evidence suggests that both subunits of heat-labile enterotoxin (LT), A (LTA) and B (LTB), play important roles in enhancing bacterial adherence. LTA increases bacterial adhesion molecules expression, while LTB mediates the initial interaction with GM1 receptors of host cells. Our study also showed that cyclic AMP (cAMP) and cholesterol play significant roles in LT-enhanced bacterial adherence.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Maria Laura Kevorkian, Salome C. Vilchez Larrea, Silvia H. Fernandez Villamil
Summary: This study identifies the nuclear localization of TcPARP and its enrichment in the nucleolus of Trypanosoma cruzi. The study also reveals the presence of TcPARP in a thread-like structure connecting two dividing nuclei, and the detection of ADP-ribose polymers in this structure during mitosis. These findings shed light on new potential functions of TcPARP inside the nucleus and contribute to our understanding of its role in trypanosomatids beyond the well-described DNA damage response.
Article
Biochemistry & Molecular Biology
Johannes Rudolph, Karolin Luger
Summary: The auxiliary factor HPF1 redirects the enzymatic activity of PARP1, altering the PAR modification of proteins and affecting DNA repair in cells.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)