Article
Immunology
Jing Jiang, Zhihong Cao, Li Xiao, Jinwen Su, Jinhe Wang, Jianqin Liang, Bingfen Yang, Yanhua Liu, Fei Zhai, Ruo Wang, Xiaoxing Cheng
Summary: Single-cell transcriptome and T cell receptor sequencing were used to identify T cell subsets associated with control of tuberculosis. Fourteen distinct subsets of T cells were identified, including a depleted GZMK-expressing CD8+ cytotoxic T cell cluster and a depleted SOX4-expressing CD4+ central memory T cell cluster. The ratio of different Granzyme-expressing T cell subsets showed correlations with the extent of tuberculosis lesions, suggesting their potential roles in protection against tuberculosis dissemination.
CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Pierre Boudinot, Samuel Novas, Luc Jouneau, Stanislas Mondot, Marie-Paule Lefranc, Unni Grimholt, Susana Magadan
Summary: T-cell mediated immunity depends on a variety of T cell receptors (TR) that are specific to antigens. Understanding the structure of TR genes is crucial for studying the diversity and composition of T cell responses in vertebrate species. However, the lack of good-quality genome assemblies and difficulties in mapping highly similar TR sequences have limited the study of these genes in non-model organisms. In this study, we characterized the TRB genes located on specific chromosomes in rainbow trout and compared them with other salmonid species. Our findings shed light on the evolution of TRB genes in salmonids and provide a basis for monitoring T cell responses in salmonids.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Lu Yang, Yanbing Zhu, Dan Tian, Song Wang, Jincheng Guo, Guangyong Sun, Hua Jin, Chunpan Zhang, Wen Shi, M. Eric Gershwin, Zhongtao Zhang, Yi Zhao, Dong Zhang
Summary: The study used single-cell RNA sequencing to analyze DNT cells, revealing transcriptional characteristics and functional roles of different subsets, which contributes to a better understanding of their roles in the immune system.
JOURNAL OF AUTOIMMUNITY
(2021)
Article
Immunology
Eva-Stina Edholm, Christopher Graham Fenton, Stanislas Mondot, Ruth H. Paulssen, Marie-Paule Lefranc, Pierre Boudinot, Susana Magadan
Summary: This study focuses on the characterization of T cell populations in salmonids, specifically rainbow trout and Atlantic salmon, which are important models for fish immunology and vaccine development. By analyzing the immune responses at a genetic level, the study aims to establish a common salmonid TRA/TRD nomenclature for accurate high-throughput sequencing results.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chong-Bin Hu, Jie Wang, Yun Hong, Hao Li, Dong-Dong Fan, Ai-Fu Lin, Li-Xin Xiang, Jian-Zhong Shao
Summary: This study generated a comprehensive atlas of immune cell types in zebrafish spleen based on single-cell transcriptome profiling. It identified 11 major categories and 54 potential subsets of immune cells that have diverse roles in antiviral immunity. The study also showed that trained immunity can be induced in specific subsets by vaccinating zebrafish with inactivated virus.
Article
Oncology
Xiaonan Wang, Carlotta Peticone, Ekaterini Kotsopoulou, Berthold Gottgens, Fernando J. Calero-Nieto
Summary: This study used single-cell transcriptomic analysis to characterize the molecular features of CAR T cell activation, revealing several subpopulations of cells in CAR products with reproducible cellular composition across donors. Targeted data interrogation also showed that a small proportion of antigen-responding CAR-expressing cells exhibited exhaustion signatures with known markers and previously unassociated genes.
Article
Biochemistry & Molecular Biology
Dingyi Yuan, Yuxin Zhang, Wanmei Liu, Xiaoyu He, Wenting Chen, Liu Liu, Lu Yang, Yixin Wang, Yinhao Wu, Jun Liu
Summary: In this study, the potential mechanisms by which VISTA affects T-cell activation and the potential interacting proteins were explored. The results showed that VISTA inhibits T-cell activation by suppressing pathways related to T-cell differentiation, T-cell receptor signaling, and T-cell migration; by influencing cholesterol biosynthesis; and by regulating inflammatory response. The study also identified HHLA2 and CTH as direct interacting partners of VISTA, and an indirect interaction between VISTA and PSGL-1.
MOLECULAR IMMUNOLOGY
(2023)
Review
Cardiac & Cardiovascular Systems
Akinori Higaki, Ahmad U. M. Mahmoud, Pierre Paradis, Ernesto L. Schiffrin
Summary: Hypertension is partially mediated by immune mechanisms, with IL-23 and IL-17 playing important roles. The IL-23/IL-17 axis has not been a major therapeutic target for hypertension treatment, but shows potential for targeting therapy.
CARDIOVASCULAR RESEARCH
(2021)
Review
Immunology
Anna Pasetto, Yong-Chen Lu
Summary: T cells are essential in immune responses and cancer immunotherapy. Single-cell sequencing techniques have enabled scientists to study T cells at a deeper level, including T-cell receptor and transcriptome analysis. These techniques also aid in the identification of T-cell neoantigens, advancing T-cell mediated cancer therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
M. Monteiro, P. Perdiguero, A. Couto, C. R. Serra, P. Pereiro, B. Novoa, A. Figueras, L. Ribeiro, P. Pousao-Ferreira, C. Tafalla, A. Oliva-Teles, P. Enes, C. J. Secombes, P. Diaz-Rosales
Summary: Meagre, a potential candidate for Mediterranean aquaculture diversification, has understudied physiological consequences of immune system activation. This study evaluated the in vivo effects of lipo-polysaccharide on the spleen transcriptome of meagre using RNA-seq analysis.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Immunology
Hui Xu, Jianping Jia
Summary: This study explored the characteristics of peripheral immune cells in Alzheimer's disease by profiling single-cell transcriptome and immune repertoire sequencing. Five immune cell subsets were identified in AD patients, with alterations in cell subset proportion and gene expression patterns. High-frequency amplification clonotypes were found in T and B cells, suggesting a potential role of adaptive immune response in the pathogenesis of AD.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Oncology
Xiuting Liu, Graham D. Hogg, David G. DeNardo
Summary: The success of checkpoint inhibitors in cancer treatment has highlighted the importance of the immune system, particularly in eliciting antitumor responses. While initially thought to primarily target T cells, recent research suggests that these inhibitors may also impact innate immunity, thus playing a significant role in clinical efficacy. Further investigation into the mechanisms of action of checkpoint therapies on innate antitumor responses is crucial for developing effective combination treatments to combat checkpoint resistance in refractory malignancies.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Multidisciplinary Sciences
Nasna Nassir, Richa Tambi, Asma Bankapur, Saba Al Heialy, Noushad Karuvantevida, Hamda Hassan Khansaheb, Binte Zehra, Ghausia Begum, Reem Abdel Hameid, Awab Ahmed, Zulfa Deesi, Abdulmajeed Alkhajeh, K. M. Furkan Uddin, Hosneara Akter, Seyed Ali Safizadeh Shabestari, Omar Almidani, Amirul Islam, Mellissa Gaudet, Richard Kumaran Kandasamy, Tom Loney, Ahmad Abou Tayoun, Norbert Nowotny, Marc Woodbury-Smith, Proton Rahman, Wolfgang M. Kuebler, Mahmood Yaseen Hachim, Jean-Laurent Casanova, Bakhrom K. Berdiev, Alawi Alsheikh-Ali, Mohammed Uddin
Summary: Through single-cell transcriptomics, a specific subtype of MoAMs associated with severe COVID-19 and upregulated genes related to severe comorbidities were identified. This subtype may serve as a novel biomarker for screening and prognosis, as well as a potential therapeutic target for severe cases.
Review
Immunology
Wenshuai Li, Xuanxuan Pan, Lirong Chen, Haoshu Cui, Shaocong Mo, Yida Pan, Yuru Shen, Menglin Shi, Jianlin Wu, Feifei Luo, Jie Liu, Na Li
Summary: Adoptive cell therapy (ACT) using chimeric antigen receptor (CAR)-modified T cells has shown remarkable efficacy against hematological malignancies, but its success in solid tumors is limited by factors such as easy recurrence and poor efficacy. The effector function and persistence of CAR-T cells are critical to the success of therapy and are modulated by metabolic and nutrient-sensing mechanisms. Moreover, the immunosuppressive tumor microenvironment (TME) can lead to T cell exhaustion and compromise the efficacy of CAR-T cells. This review discusses the metabolic characteristics of T cells and potential metabolic approaches to improve the efficacy and persistence of CAR-T cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Carlo C. Lazado, Marianne Iversen, Arvind Y. M. Sundaram
Summary: This study provides the first insight into the basal transcriptome of the olfactory rosette in Atlantic salmon. Comparative transcriptomic analysis with gills, another organ possessing MALT, reveals shared cytokine-cytokine interaction and neuroactive ligand-receptor interaction pathways. The immunological characteristics of both organs are further characterized by the overrepresentation of immune-related pathways, particularly important for pathogen recognition. The differential regulation of markers for interleukins, extracellular matrix, antimicrobial peptides, and complement highlights the immunological differences between the two organs. The basal transcriptome of the Atlantic salmon olfactory rosette is a valuable molecular toolbox for understanding nasal immunity in teleost fish.
Article
Biochemistry & Molecular Biology
Xinru Li, Tao Wang, Shaowu Yin, Guosong Zhang, Quanquan Cao, Xin Wen, Hongye Zhang, Dan Wang, Wenxu Zhu
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Dan Wang, Quanquan Cao, Wenxu Zhu, Yadong Hu, Xinyu Zhang, Shaowu Yin, Tao Wang
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Wenfeng Xiong, Shanshan Gao, Yaoyao Lu, Luting Wei, Jinjuan Mao, Jia Xie, Quanquan Cao, Juanjuan Liu, Jingxiu Bi, Xiaowen Song, Bin Li
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2019)
Article
Genetics & Heredity
Quanquan Cao, Peng Chu, Jie Gu, Hongyan Zhang, Runhua Feng, Xin Wen, Dan Wang, Wenfeng Xiong, Tao Wang, Shaowu Yin
Article
Environmental Sciences
Quanquan Cao, Shaowu Yin
Summary: This study focused on the morphological changes of gill tissues in eels when faced with environmental calcium challenges, showing differences in filament and lamella lengths, lamellar frequency, and lamella width in different calcium environments. Ultrastructural changes in gills were examined using transmission electron microscopy, indicating expansion in calcium-deficient water and shrinkage in high-calcium water compared to the control group. Branchial surface areas are regulated in different calcium waters through calcium transporters such as CACNB2.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Quanquan Cao, Ivone Giffard-Mena, Eva Blondeau-Bidet, Sophie Hermet, Yau-Chung Hu, Tsung-Han Lee, Catherine Lorin-Nebel
Summary: The study on the kidney function of European sea bass in high salinity conditions revealed slight differences in response to high salinity between the two genetic lineages, with Mediterranean sea bass showing high blood osmolality in hypersaline water but not showing improved overall response compared to the Atlantic lineage. At the kidney level, the traits analyzed differ slightly between genetic lineages, potentially as a response to high blood osmolalities in Mediterranean sea bass.
Review
Oncology
Sijing Li, Xiaohui Wei, Jinyong He, Quanquan Cao, Danyu Du, Xiaoman Zhan, Yuqi Zeng, Shengtao Yuan, Li Sun
Summary: miR-34 plays crucial roles in cancer, with its downstream targets involved in multiple signaling pathways and crucial biological processes. Among them, miR-34a is especially important and can effectively suppress tumor progression by combining with chemotherapy or radiotherapy.
CANCER AND METASTASIS REVIEWS
(2021)
Article
Fisheries
Quanquan Cao, Jie Li, Yiru Sun, Daoqiang Geng, Peng Chu, Hongyu Wang, Shaowu Yin
Summary: The study demonstrated that there are significant changes in osmoregulatory mechanisms in marbled eels when transferred from fresh water to brackish water and saline water, with levels of plasma ions, osmolality, and cortisol consistently higher in saline water than brackish water, indicating efficient acclimation to higher salinities and increased active ion transport and cortisol synthesis in the gill of the eels.
Article
Environmental Sciences
Quanquan Cao, Eva Blondeau-Bidet, Catherine Lorin-Nebel
Summary: European sea bass migrate to habitats with high salinity levels and show different physiological responses to hypersaline conditions among different lineages, suggesting slight physiological differences between populations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Quanquan Cao, Eva Blondeau-Bidet, Catherine Lorin-Nebel
Summary: This study compared the expression of neuropeptide receptors and isotocin receptors in the intestines of different genetic lineages of European sea bass under different salinity environments. The results showed significant differences in gene expression between genetic lineages, which may contribute to different responses to osmotic stress in D. labrax lineages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ju Zhao, Ye Zhao, Haifeng Liu, Quanquan Cao, Lin Feng, Zhihao Zhang, Weidan Jiang, Pei Wu, Yang Liu, Wei Luo, Xiaoli Huang, Jun Jiang
Summary: This study aimed to evaluate the possible impact and mechanism of leucine on fish intestinal barrier function. The results showed that the intestinal activities of certain enzymes and the levels of immune proteins had positive responses to dietary leucine levels. These findings suggest that leucine can improve fish intestinal barrier function by enhancing humoral immunity, antioxidative capacities, and tight junction protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biology
Quanquan Cao, Hailong Zhang, Tong Li, Lingjie He, Jiali Zong, Hongying Shan, Lishi Huang, Yupeng Zhang, Haifeng Liu, Jun Jiang
Summary: miRNA is a class of endogenous and evolutionarily conserved noncoding short RNA molecules that post-transcriptionally regulate gene expression through sequence-specific interactions with mRNAs and are capable of controlling gene expression by binding to miRNA targets and interfering with the final protein output. Recent studies have focused on the characteristics and functions of miRNAs in fish, while the understanding of these small molecules has expanded significantly in environmental stress mechanisms in the past few years. Environmental factors may influence the transcriptional and posttranscriptional regulators of miRNAs, contributing to nearly all biological processes.
Article
Biochemistry & Molecular Biology
Haifeng Liu, Jie Wen, Xue Tian, Tong Li, Ju Zhao, Jingjing Cheng, Lishi Huang, Ye Zhao, Quanquan Cao, Jun Jiang
Summary: This study found that FSTL1 is highly expressed in the early stage of adipocyte differentiation and plays a possible role in adipogenesis. The study also revealed that miR-125a-3p negatively regulates FSTL1 and affects adipocyte differentiation and inflammation. These findings contribute to a better understanding and treatment of obesity-related inflammatory diseases.
Article
Fisheries
Quanquan Cao, Ju Zhao, Mingyao Yan, Zhong Luo, Fu Luo, Lin Feng, Weidan Jiang, Pei Wu, Yan Wang, Debin Li, Haifeng Liu, Jun Jiang
Summary: This study investigates the effects of Vitamin D3 (VD3) on the immune response and xenophagy of largemouth bass after Nocardia seriolae challenge. The results demonstrate that VD3 can enhance the innate immune response and xenophagy through VDR-mediated immunomodulation, contributing to the resistance of largemouth bass to N. seriolae infection.
Article
Biochemistry & Molecular Biology
Diana Medina-Felix, Francisco Vargas-Albores, Estefania Garibay-Valdez, Luis Rafael Martinez-Cordova, Marcel Martinez-Porchas
Summary: In this research, the effects of Nocardia infection on fish gastrointestinal microbiota were analyzed. It was found that the infection led to decreased survival rate, severe damage to the stomach microbiota, and a significant increase in Proteobacteria. A negative correlation network between Proteobacteria and other important phyla was observed. Therefore, Nocardia sp. is an emerging pathogen capable of inducing dysbiosis and causing significant mortalities.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
Article
Biochemistry & Molecular Biology
Lele Wu, Wen Sun, Jiale Zhou, Yaolin Li, Jun Li, Zongcheng Song, Changbin Song, Shihong Xu, Xinlu Yue, Xian Li
Summary: The study finds that red light induces dichromatic skin pigmentation in turbot juveniles, with some individuals displaying black coloration and others displaying lighter skin. The upregulated gene expressions related to melanin synthesis and the involvement of the nervous system in spectral environment-driven color regulation are both crucial factors.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2024)
