Article
Agronomy
Yonglei Zhang, Runnan Yu, Jing Tang, Liheng Du, Yihan Wang, Jiatao Wang, Linsu Liu, Shanshan Gao, Bin Li
Summary: Three insect cytochrome P450 monooxygenase genes (TcCYP4Q3, TcCYP4Q5, and TcCYP4Q7) in Tribolium castaneum are regulated by the CncC signaling pathway and play a role in the susceptibility of the beetles to eugenol, a phytochemical. These findings have implications for the development of novel therapeutics to control pests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Mei Bai, Zi-Ling Liu, Yu-Yu Zhou, Qiu-Xuan Xu, Tong-Xian Liu, Hong-Gang Tian
Summary: Our study evaluated the effects of different dsRNA storage conditions on RNAi efficiency. The results showed that the dsRNA molecule was highly stable under typical cryopreservation temperatures and multiple freeze-thaw cycles, and there were no significant differences in RNAi efficiency. Surprisingly, dsRNA remained stable and induced an efficient RNAi response even under refrigerated storage conditions.
PEST MANAGEMENT SCIENCE
(2023)
Article
Entomology
Yonglei Zhang, Jiahao Zhang, Dongyu Li, Haidi Sun, Ruixue Lu, Se Yin, Xinlong Guo, Shanshan Gao
Summary: In this study, three TcAOX genes (TcAOX1, TcAOX2, TcAOX3) from Tribolium castaneum genome were identified and characterized. The results showed that TcAOX1 is involved in the degradation of chemical signals, while TcAOX2 and TcAOX3 are associated with plant toxicant detoxification. Knockdown of TcAOX1 led to a reduction in female egg-laying, suggesting its role in fecundity.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Genetics & Heredity
Juanjuan Liu, Shanshan Gao, Luting Wei, Wenfeng Xiong, Yaoyao Lu, Xiaowen Song, Yonglei Zhang, Han Gao, Bin Li
Summary: This study investigates the functions of choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT) in insect development, reproduction, and insecticide susceptibility. The researchers found that RNA interference of Chat and vacht in Tribolium castaneum resulted in decreased acetylcholine content and disrupted larval development and pupal eclosion. Knockdown of these enzymes also reduced fecundity and egg hatchability. Additionally, the researchers observed that the transcript levels of chat and vacht increased after treatment with carbofuran or dichlorvos, and reduction of chat or vacht decreased the resistance to these insecticides.
Article
Biochemistry & Molecular Biology
Shanshan Gao, Ruixue Lu, Yonglei Zhang, Haidi Sun, Siying Li, Kunpeng Zhang, Ruimin Li
Summary: The study found that the OBP gene OBPC12 of Tribolium castaneum may be involved in the insect's defense response to exogenous toxic substances, leading to a decrease in sensitivity to eugenol; The expression pattern of TcOBPC12 in different tissues and developmental stages varies, indicating its potential important role in larvae absorbing exogenous toxic substances.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Shanshan Gao, Shumei Liu, Tian Gao, Xinyi Zhang, Zhuangkun Huo, Kunpeng Zhang
Summary: The study reveals that Vesicular acetylcholine transporter (vacht) plays a crucial role in the metamorphosis, reproduction, and insecticide susceptibility of Tribolium castaneum. Transcriptome profiling after Tcvacht downregulation through RNAi in T. castaneum larvae identified 479 differentially expressed genes (DEGs), including those involved in detoxification processes, immune pathways, and metabolic genes. Knockdown of Tcvacht expression resulted in decreased expression of enzymes involved in detoxification and increased susceptibility to insecticides. These findings provide valuable insights into the biological processes influenced by Tcvacht and potential strategies for biological control of T. castaneum.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Runnan Yu, Wenjing Zhang, Yanxiao Li, Jing Tang, KumChol Kim, Bin Li
Summary: ALKBH4 plays a crucial role in epigenetic regulation in mammals by repairing alkyl lesions, but its function in insects is not well understood. In this study, the TcALKBH4 gene was cloned and characterized in Tribolium castaneum. The expression pattern of TcALKBH4 showed high expression in early embryos and early pupae, and in the adult testis and ovary. RNA interference targeting TcALKBH4 at different developmental stages resulted in distinct phenotypes, including developmental failure in larvae, reduced food intake, and fertility deficiency in adults. However, TcALKBH4 RNAi did not affect 6mA levels in vivo. qRT-PCR analysis suggested that TcALKBH4 may mediate larval development through the 20E signaling pathway, and the fertility of female and male adults may be regulated by vitellogenesis and JH signaling pathways, respectively. These findings provide new insights into the potential function of ALKBH4 in insects.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kun Qian, Yi Yang, Changyin Zhou, Haoyu Zhang, Nan Zhang, Xiangkun Meng, Jianjun Wang
Summary: In this study, two putative sodium channel genes, TcNav1 and TcNav2, were cloned and characterized in Tribolium castaneum, encoding proteins with amino acid similarities to Drosophila para. Knockdown of TcNav1 and TcNav2 adversely affected adult emergence and decreased sensitivity to insecticides. These findings suggest that duplication of sodium channel genes in T. castaneum has led to diversified developmental and toxicological functions.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Shanshan Gao, Xinlong Guo, Shumei Liu, Siying Li, Jiahao Zhang, Shuang Xue, Qingbo Tang, Kunpeng Zhang, Ruimin Li
Summary: This study identified a P450 gene (CYP6BQ8) from Tribolium castaneum and investigated its expression profile and potential role in the detoxification of terpinen-4-ol. The results showed that TcCYP6BQ8 expression was higher in early- and late-larval stages and mainly expressed in the head and integument of larvae and adults. RNA interference against TcCYP6BQ8 increased terpinen-4-ol-induced larval mortality, suggesting its involvement in T. castaneum's metabolism of terpinen-4-ol.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yuan-chen Zhang, Shan-shan Gao, Shuang Xue, Shi-heng An, Kun-peng Zhang
Summary: The study demonstrates that the CYP6BQ7 gene in Tribolium castaneum plays a key role in detoxifying plant toxicants, with its expression induced by Artemisia vulgaris essential oil. Higher expression in larval and adult stages, particularly in the brain and hemolymph, suggests pivotal involvement in detoxification. RNA interference silencing of TcCYP6BQ7 increased larvae mortality in response to essential oil, indicating its importance in detoxification.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biology
Marleen Klann, Magdalena Ines Schacht, Matthew Alan Benton, Angelika Stollewerk
Summary: Insects and other arthropods use external sensory structures for mechanosensory, olfactory, and gustatory reception, with sense organs having characteristic shapes related to their functions. While Drosophila melanogaster regulates sense organ identity through specific combinations of transcription factors, other arthropods do not link sense organ subtypes to the same gene expression code. Sense organ diversity may have evolved differently across insect species, with recruitment of sensory genes to distinct sensory organ specification mechanisms playing a role.
Article
Agronomy
In-Young Kim, Byungyoon Choi, Woo-Ram Park, Yu-Ji Kim, Bo-Eun Kim, Seulgi Mun, Hueng-Sik Choi, Don-Kyu Kim
Summary: The study revealed the crucial role of TcHR96 in transcriptional control of P450 enzyme genes for imidacloprid detoxification in T. castaneum. Targeting TcHR96 could potentially help regulate insecticide tolerance in pest beetle populations.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shanshan Gao, Kui Liu, Hui Liu, Se Yin, Xinlong Guo, Yonglei Zhang, Kunpeng Zhang, Ruimin Li
Summary: In this study, a P450 gene (CYP9Z6) was identified from Tribolium castaneum and its role in detoxifying terpinen-4-ol was investigated. The results showed that TcCYP9Z6 plays a significant role in the detoxification of terpinen-4-ol, which suggests its potential as a target for RNAi-mediated prevention and control of T. castaneum.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Entomology
Fazalullah M. Bughio, Richard M. Wilkins
Summary: The study revealed that the resistant strain of the red flour beetle had smaller gut dimensions, consumed less food, and had lower larval protein content, but higher adult protein content. This may be associated with malathion resistance.
JOURNAL OF STORED PRODUCTS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Nan Zhang, Jiaping Wei, Heng Jiang, Huichen Ge, Yang Zheng, Xiangkun Meng, Kun Qian, Jianjun Wang
Summary: This study found that arginine kinase (AK) plays an important role in insect response to insecticides by regulating cellular energy balance and participating in detoxification.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xinru Li, Tao Wang, Shaowu Yin, Guosong Zhang, Quanquan Cao, Xin Wen, Hongye Zhang, Dan Wang, Wenxu Zhu
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Dan Wang, Quanquan Cao, Wenxu Zhu, Yadong Hu, Xinyu Zhang, Shaowu Yin, Tao Wang
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2019)
Article
Genetics & Heredity
Quanquan Cao, Peng Chu, Jie Gu, Hongyan Zhang, Runhua Feng, Xin Wen, Dan Wang, Wenfeng Xiong, Tao Wang, Shaowu Yin
Article
Environmental Sciences
Quanquan Cao, Shaowu Yin
Summary: This study focused on the morphological changes of gill tissues in eels when faced with environmental calcium challenges, showing differences in filament and lamella lengths, lamellar frequency, and lamella width in different calcium environments. Ultrastructural changes in gills were examined using transmission electron microscopy, indicating expansion in calcium-deficient water and shrinkage in high-calcium water compared to the control group. Branchial surface areas are regulated in different calcium waters through calcium transporters such as CACNB2.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Quanquan Cao, Ivone Giffard-Mena, Eva Blondeau-Bidet, Sophie Hermet, Yau-Chung Hu, Tsung-Han Lee, Catherine Lorin-Nebel
Summary: The study on the kidney function of European sea bass in high salinity conditions revealed slight differences in response to high salinity between the two genetic lineages, with Mediterranean sea bass showing high blood osmolality in hypersaline water but not showing improved overall response compared to the Atlantic lineage. At the kidney level, the traits analyzed differ slightly between genetic lineages, potentially as a response to high blood osmolalities in Mediterranean sea bass.
Review
Oncology
Sijing Li, Xiaohui Wei, Jinyong He, Quanquan Cao, Danyu Du, Xiaoman Zhan, Yuqi Zeng, Shengtao Yuan, Li Sun
Summary: miR-34 plays crucial roles in cancer, with its downstream targets involved in multiple signaling pathways and crucial biological processes. Among them, miR-34a is especially important and can effectively suppress tumor progression by combining with chemotherapy or radiotherapy.
CANCER AND METASTASIS REVIEWS
(2021)
Article
Fisheries
Quanquan Cao, Jie Li, Yiru Sun, Daoqiang Geng, Peng Chu, Hongyu Wang, Shaowu Yin
Summary: The study demonstrated that there are significant changes in osmoregulatory mechanisms in marbled eels when transferred from fresh water to brackish water and saline water, with levels of plasma ions, osmolality, and cortisol consistently higher in saline water than brackish water, indicating efficient acclimation to higher salinities and increased active ion transport and cortisol synthesis in the gill of the eels.
Article
Biochemistry & Molecular Biology
Quanquan Cao, Hongyu Wang, Chengxu Fan, Yiru Sun, Jie Li, Jinghao Cheng, Peng Chu, Shaowu Yin
Summary: The study found that environmental salinity changes affect the immune system of fish, particularly genes related to the TCR pathway. Analysis of transcripts from gills revealed significant differences in gene expression in different salinity environments, implicating these genes in the regulation of immune signaling pathways.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
(2021)
Article
Environmental Sciences
Quanquan Cao, Eva Blondeau-Bidet, Catherine Lorin-Nebel
Summary: European sea bass migrate to habitats with high salinity levels and show different physiological responses to hypersaline conditions among different lineages, suggesting slight physiological differences between populations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Quanquan Cao, Eva Blondeau-Bidet, Catherine Lorin-Nebel
Summary: This study compared the expression of neuropeptide receptors and isotocin receptors in the intestines of different genetic lineages of European sea bass under different salinity environments. The results showed significant differences in gene expression between genetic lineages, which may contribute to different responses to osmotic stress in D. labrax lineages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ju Zhao, Ye Zhao, Haifeng Liu, Quanquan Cao, Lin Feng, Zhihao Zhang, Weidan Jiang, Pei Wu, Yang Liu, Wei Luo, Xiaoli Huang, Jun Jiang
Summary: This study aimed to evaluate the possible impact and mechanism of leucine on fish intestinal barrier function. The results showed that the intestinal activities of certain enzymes and the levels of immune proteins had positive responses to dietary leucine levels. These findings suggest that leucine can improve fish intestinal barrier function by enhancing humoral immunity, antioxidative capacities, and tight junction protein levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biology
Quanquan Cao, Hailong Zhang, Tong Li, Lingjie He, Jiali Zong, Hongying Shan, Lishi Huang, Yupeng Zhang, Haifeng Liu, Jun Jiang
Summary: miRNA is a class of endogenous and evolutionarily conserved noncoding short RNA molecules that post-transcriptionally regulate gene expression through sequence-specific interactions with mRNAs and are capable of controlling gene expression by binding to miRNA targets and interfering with the final protein output. Recent studies have focused on the characteristics and functions of miRNAs in fish, while the understanding of these small molecules has expanded significantly in environmental stress mechanisms in the past few years. Environmental factors may influence the transcriptional and posttranscriptional regulators of miRNAs, contributing to nearly all biological processes.
Article
Biochemistry & Molecular Biology
Haifeng Liu, Jie Wen, Xue Tian, Tong Li, Ju Zhao, Jingjing Cheng, Lishi Huang, Ye Zhao, Quanquan Cao, Jun Jiang
Summary: This study found that FSTL1 is highly expressed in the early stage of adipocyte differentiation and plays a possible role in adipogenesis. The study also revealed that miR-125a-3p negatively regulates FSTL1 and affects adipocyte differentiation and inflammation. These findings contribute to a better understanding and treatment of obesity-related inflammatory diseases.
Article
Fisheries
Quanquan Cao, Ju Zhao, Mingyao Yan, Zhong Luo, Fu Luo, Lin Feng, Weidan Jiang, Pei Wu, Yan Wang, Debin Li, Haifeng Liu, Jun Jiang
Summary: This study investigates the effects of Vitamin D3 (VD3) on the immune response and xenophagy of largemouth bass after Nocardia seriolae challenge. The results demonstrate that VD3 can enhance the innate immune response and xenophagy through VDR-mediated immunomodulation, contributing to the resistance of largemouth bass to N. seriolae infection.