Article
Physiology
Adan Dagnino-Acosta, Agustin Guerrero-Hernandez
Summary: PKC inhibitors stimulate Ca2+ release from internal stores by increasing SR Ca2+ leak mediated by translocon activation. This increased leak does not deplete the Ca2+ store, but rather compensates by increasing SERCA pump activity, leading to a new steady-state level. This change also results in increased activity of high conductance, Ca2+-sensitive potassium channels.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Physiology
Jan B. Parys, Fabien Van Coppenolle
Summary: In this review, the structure, pharmacology, and regulation of the Sec61 complex as a Ca2+-leak channel are discussed. The pathologies linked to Sec61 mutations and the importance of the Sec61 complex as a Ca2+-leak channel in various pathophysiological and pathological settings are also explored.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Neurosciences
Pragnya Chakraborty, Gaiti Hasan
Summary: Key components of endoplasmic reticulum (ER) Ca2+ release and store-operated Ca2+ entry (SOCE) are expressed in all metazoan cells. This review presents evidence on how these two mechanisms impact neuronal physiology and their interaction with ion channels in neurons. The careful regulation of neural SOCE is essential due to the detrimental effects of Ca2+ imbalance on neurons. The study also discusses different mechanisms of SOCE regulation in Drosophila and mammalian neurons, and their significance in human neurodegenerative diseases.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Cell Biology
Fernanda O. Lemos, Ian de Ridder, Martin D. Bootman, Geert Bultynck, Jan B. Parys
Summary: PKM2 regulates calcium handling in the cytosol and mitochondria separately, while TAT-D5SD induces apoptosis by disrupting the PKM2:IP3R interaction.
Review
Physiology
Pura Bolanos, Juan C. Calderon
Summary: The excitation-contraction coupling in skeletal muscle is a crucial link between membrane excitation and mechanical contraction mediated by Ca2+, involving multiple mechanisms of Ca2+ release and recycling.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zhanat Koshenov, Furkan E. Oflaz, Martin Hirtl, Johannes Pilic, Olaf A. Bachkoenig, Benjamin Gottschalk, Corina T. Madreiter-Sokolowski, Rene Rost, Roland Malli, Wolfgang F. Graier
Summary: The endoplasmic reticulum (ER) is a vital organelle responsible for processing and trafficking nearly 30% of human proteins. ER stress can trigger a response called unfolded protein response (UPR) to restore ER functions. The Sigma-1 receptor (S1R) plays a crucial role in enhancing mitochondrial bioenergetics during early ER stress by directing enhanced ER Ca2+ leak towards mitochondria.
Article
Cell Biology
Crystal Seng, Luke Pearce, Aldo Meizoso-Huesca, Daniel P. Singh, Robyn M. Murphy, Cedric R. Lamboley, Bradley S. Launikonis
Summary: Ca2+ plays an important role in the functional and developmental regulation of mitochondria, regulating ATP resynthesis rate, the expression of PGC1a, and the generation of reactive oxygen species in skeletal muscle. Understanding the triggers for Ca2+ redistribution between intracellular compartments is crucial for determining mitochondrial Ca2+ load.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Cell Biology
Ian de Ridder, Martijn Kerkhofs, Fernanda O. Lemos, Jens Loncke, Geert Bultynck, Jan B. Parys
Summary: Endoplasmic reticulum (ER)-mitochondria contact sites are crucial for Ca2+ flux and involve multiple proteins. Inositol 1,4,5-trisphosphate receptors (IP3Rs) play a central role in tethering ER and mitochondria and transporting Ca2+. The IP3R-GRP75-VDAC1 complex acts as a hub for stabilizing and regulating Ca2+ transfer into mitochondria.
Review
Physiology
Annemarie Schulte, Robert Blum
Summary: Cells maintain resting Ca2+ levels through dynamic Ca2+ fluxes, where ER Ca2+ leak plays a crucial role.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Claude Collet, Mercedes Charreton, Laszlo Szabo, Marianna Takacs, Laszlo Csernoch, Peter Szentesi
Summary: Calcium sparks in honey bee skeletal muscle cells show higher frequency and larger spatial spread compared to vertebrates, with both sparks and embers present. The differences in spark amplitudes between honey bees and vertebrates may be related to genomic and excitation-contraction coupling specificities. The characterization of calcium release events in an arthropod like honey bees, with distinct genomic, ultrastructural, and physiological features, may contribute to a better understanding of calcium homeostasis in invertebrate cells.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Jeffrey T. Lock, Ian Parker
Summary: The study found that individual puffs contribute about 40% of the total Ca2+ liberation during global Ca2+ elevations, with the major factor terminating punctate Ca2+ release being a decline in puff frequency. While the amplitudes of large puffs decrease during the flurry, the amplitudes of smaller puffs remain steady, resulting in a modest overall decline in puff amplitudes (around 30%).
Article
Cardiac & Cardiovascular Systems
Yuxuan Guo, Yangpo Cao, Blake D. Jardin, Xiaoran Zhang, Pingzhu Zhou, Silvia Guatimosim, Junsen Lin, Zhan Chen, Yueyang Zhang, Neil Mazumdar, Fujian Lu, Qing Ma, Yao-Wei Lu, Mingming Zhao, Da-Zhi Wang, Erdan Dong, William T. Pu
Summary: The aim of this study is to investigate the role of RYR2 in cardiomyocyte maturation. The researchers used genetic editing techniques to knockout RYR2 and found that its depletion led to structural and transcriptional defects in cardiomyocytes, along with activation of ER stress pathways. The defects in RYR2-depleted cardiomyocytes were partially rescued by an ER stress alleviator.
CARDIOVASCULAR RESEARCH
(2023)
Article
Developmental Biology
Rishav Mitra, Shlesha Richhariya, Siddharth Jayakumar, Dimple Notani, Gaiti Hasan
Summary: In fruit fly larvae, the IP3/Ca2+ signals in specific interneurons drive the expression of Set2 gene, essential for pupariation in the absence of dietary protein. This signal-controlled expression of Set2 upregulates key Ca2+-signaling genes through a positive-feedback loop, regulating gene expression for the transition from larval to pupal stages.
Article
Cardiac & Cardiovascular Systems
Jinhong Wei, Wenting Guo, Ruiwu Wang, John Paul Estillore, Darrell Belke, Yong-Xiang Chen, Alexander Vallmitjana, Raul Benitez, Leif Hove-Madsen, S. R. Wayne Chen
Summary: The physiological significance of PKA phosphorylation of RyR2 in the heart is still poorly understood. Recent structural studies have shown that the PKA phosphorylation site S2030 in RyR2 is located within a pathway that is important for the termination of Ca2+ release. We investigated the impact of S2030 mutations on Ca2+ release termination in cells and generated a mouse model to study the role of S2030 in a physiological setting.
CIRCULATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Fan Yang, Hongwei Ma, Michael R. Butler, Xi-Qin Ding
Summary: This study investigated how preserving ER Ca2+ stores leads to cone protection through cone-specific deletion of IP(3)R1, demonstrating that the absence of IP(3)R1 improves protein localization and reduces ER stress and cone death. The findings highlight the importance of ER Ca2+ stores in ER proteostasis and protein trafficking in photoreceptors, supporting the potential of IP(3)R1 as a critical mediator in cone protection.
Review
Cell Biology
Peter Vandenabeele, Geert Bultynck, Savvas N. Savvides
Summary: Regulated cell death (RCD) relies on the activation and recruitment of pore-forming proteins (PFPs) to execute specific cell death pathways. This review discusses the structural rearrangements incurred by RCD-related PFPs and describes the mechanisms behind the conversion from an autoinhibited state to a membrane-embedded state. It also highlights the formation and maturation of membrane pores and the implications of different cell death modalities on physiological and pathophysiological processes.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Geert Bultynck, Shazia Khan, M. Lienhard Schmitz
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2023)
Article
Cell Biology
Ian de Ridder, Martijn Kerkhofs, Fernanda O. Lemos, Jens Loncke, Geert Bultynck, Jan B. Parys
Summary: Endoplasmic reticulum (ER)-mitochondria contact sites are crucial for Ca2+ flux and involve multiple proteins. Inositol 1,4,5-trisphosphate receptors (IP3Rs) play a central role in tethering ER and mitochondria and transporting Ca2+. The IP3R-GRP75-VDAC1 complex acts as a hub for stabilizing and regulating Ca2+ transfer into mitochondria.
Article
Neurosciences
D. Chernyuk, M. Callens, M. Polozova, A. Gordeev, M. Chigriai, A. Rakovskaya, A. Ilina, E. Pchitskaya, C. Van den Haute, T. Vervliet, G. Bultynck, I. Bezprozvanny
Summary: Alzheimer's disease is the most common cause of dementia, and dysregulation of intracellular Ca2+ signaling is an early feature in the pathology. This study examined the hypothesis that expression of Bcl-2 proteins can normalize dysregulated Ca2+ signaling in a mouse model of AD and potentially prevent or slow the progression of the disease. The results indicate that Bcl-2 protein expression leads to synaptoprotective and amyloid-protective effects in the mouse model.
IBRO NEUROSCIENCE REPORTS
(2023)
Editorial Material
Cell Biology
Jan B. Parys, Geert Bultynck
Article
Cell Biology
Julius Ronkko, Yago Rodriguez, Tiina Rasila, Ruben Torregrosa-Munumer, Jana Pennonen, Jouni Kvist, Emilia Kuuluvainen, Ludo Van Den Bosch, Ville Hietakangas, Geert Bultynck, Henna Tyynismaa, Emil Ylikallio
Summary: Through the development of single and triple Inositol 1,4,5-trisphosphate receptors (IP3Rs) knockouts in human induced pluripotent stem cells (hiPSC), it was found that IP3Rs are not essential for hiPSC identity and pluripotency, but regulate mitochondrial metabolism. This set of knockout hiPSC is a valuable resource for investigating IP3Rs in human cell types of interest.
Article
Cell Biology
Flore Sneyers, Martijn Kerkhofs, Femke Speelman-Rooms, Kirsten Welkenhuyzen, Rita La Rovere, Ahmed Shemy, Arnout Voet, Guy Eelen, Mieke Dewerchin, Stephen W. G. Tait, Bart Ghesquiere, Martin D. Bootman, Geert Bultynck
Summary: Intracellular Ca2+ signals play a crucial role in various cellular processes. Research has shown that BAPTAi can induce apoptosis in cancer cells by inhibiting mTORC1 activity and impairing glycolysis, independent of Ca2+ signaling. Additionally, direct inhibition of PFKFB3 emerges as a potential therapeutic target in MCL-1-dependent cancers.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Elzbieta Kania, Jaclyn S. Long, David G. McEwan, Kirsten Welkenhuyzen, Rita La Rovere, Tomas Luyten, John Halpin, Evy Lobbestael, Veerle Baekelandt, Geert Bultynck, Kevin M. Ryan, Jan B. Parys
Summary: Mutations in the LRRK2 gene are the most common genetic cause of Parkinson's disease, and they also have importance in Crohn's disease and cancer. A study found that the phosphorylation status of LRRK2 affects its function, and the S910/S935/S955/S973 phosphorylation sites play a crucial role in regulating LRRK2-mediated autophagy. Cells with quadruple LRRK2 phosphomutations showed impaired autophagy during starvation, while treatment with LRRK2 kinase inhibitors did not affect autophagy. Increased LRRK2 kinase activity was found to drive autophagy impairment through phosphorylation of downstream targets Rab8a and Rab10.
CELL DEATH & DISEASE
(2023)
Article
Cell Biology
Fernanda O. Lemos, Ian de Ridder, Martin D. Bootman, Geert Bultynck, Jan B. Parys
Summary: PKM2 regulates calcium handling in the cytosol and mitochondria separately, while TAT-D5SD induces apoptosis by disrupting the PKM2:IP3R interaction.
Article
Medicine, Research & Experimental
Luc Leybaert, Maarten A. J. De Smet, Alessio Lissoni, Rosalie Allewaert, H. Llewelyn Roderick, Geert Bultynck, Mario Delmar, Karin R. Sipido, Katja Witschas
Summary: This paragraph mainly discusses the role of connexins in cardiac function. Connexins form hemichannels and gap junctions, and gap junctions are responsible for transmitting electrical and chemical signals between myocardial cells and specialized conduction system cells to synchronize the cardiac cycle and control cardiac pump function. Under pathological conditions, gap junctions close and hemichannels open, leading to disruption of cardiac function and homeostasis. Current evidence shows that hemichannels play an emerging role in myocardial ischemia and arrhythmia, and there are now tools available to selectively inhibit hemichannels without inhibiting gap junctions, as well as to stimulate the incorporation of hemichannels into gap junctions. We review experimental evidence for the contribution of hemichannels to pro-arrhythmic events in ventricular and atrial cardiomyocytes, and link these findings to the molecular control of connexin-43-based hemichannel opening. We conclude that a double-edged approach of both preventing hemichannel opening and preserving gap junctional function will be crucial for further research and development of new connexin-based experimental approaches for treating heart disease.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Immunology
Julika Neumann, Erika Van Nieuwenhove, Lara E. Terry, Frederik Staels, Taylor R. Knebel, Kirsten Welkenhuyzen, Kourosh Ahmadzadeh, Mariah R. Baker, Margaux Gerbaux, Mathijs Willemsen, John S. Barber, Irina I. Serysheva, Liesbeth De Waele, Francois Vermeulen, Susan Schlenner, Isabelle Meyts, David Yule, Geert Bultynck, Rik Schrijvers, Stephanie Humblet-Baron, Adrian Liston
Summary: Calcium signaling is crucial for lymphocyte activation, and disruptions of store-operated calcium entry can lead to severe immunodeficiency. In this study, genetic variations of the ITPR3 gene were identified in two Caucasian patients with immunodeficiency, indicating a potential link between these variations and impaired immune responses. The findings demonstrate the functional connection between defective endoplasmic reticulum calcium channels and immunodeficiency, highlighting the importance of IP(3)Rs as diagnostic targets for patients with specific inborn errors of immunity.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Correction
Immunology
Julika Neumann, Erika Van Nieuwenhove, Lara E. Terry, Frederik Staels, Taylor R. Knebel, Kirsten Welkenhuyzen, Kourosh Ahmadzadeh, Mariah R. Baker, Margaux Gerbaux, Mathijs Willemsen, John S. Barber, Irina I. Serysheva, Liesbeth De Waele, Francois Vermeulen, Susan Schlenner, Isabelle Meyts, David I. Yule, Geert Bultynck, Rik Schrijvers, Stephanie Humblet-Baron, Adrian Liston
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)