Article
Nanoscience & Nanotechnology
Yuyang Wang, Paul E. D. Soto Rodriguez, Laura Woythe, Samuel Sanchez, Josep Samitier, Peter Zijlstra, Lorenzo Albertazzi
Summary: Nanoparticles have great potential for applications in nanomedicine and molecular biosensing. The protein corona formed due to nonspecific interactions between particles and proteins plays a vital role in the fate of nanoparticles in vivo and affects their performance as biosensors. In this study, a super-resolution microscopic approach called stimulated emission depletion (STED) microscopy was used to investigate the protein corona on single silica nanoparticles and their interactions with cells. The results showed that STED microscopy can reveal the structural features of the protein corona, such as its distribution on the particle surface and the extent of protein internalization in porous particles. Moreover, the composition of the protein corona at the single-particle level was determined using multicolor measurements. It was also found that the composition varies between particles and is influenced by the particle geometry. The study demonstrates the potential of STED microscopy in understanding the mechanistic aspects of protein coronas and in the design of nanoparticles for nanomedicine and biosensing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biology
Jigmi Basumatary, Neptune Baro, Prakash Joshi, Partha Pratim Mondal
Summary: Single-molecule localization microscopy (SMLM) is a powerful technique that improves spatial resolution beyond the diffraction limit and allows for the analysis of biological features at the nanoscale. This study introduces a single molecule-based scanning SMLM (scanSMLM) system that enables rapid volume imaging. By rapidly changing the aperture of an electrically-tunable lens, the system achieves selective imaging of the axial object plane, allowing for volume scanning. The technique is employed to visualize the architecture of actin-filaments, the distribution of Meos-Tom20 molecules on the mitochondrial membrane, and the clustering of Hemagglutinin (HA) protein single molecules for studying Influenza-A disease progression. The system enables 3D visualization of HA distribution, providing unprecedented insights into the disease progression at the single-molecule level.
COMMUNICATIONS BIOLOGY
(2023)
Article
Optics
Zhengyi Zhan, Chuankang Li, Xin Liu, Xiaofan Sun, Chenyin He, Cuifang Kuang, Xu Liu
Summary: This study presents a technique for simultaneously estimating the position and orientation of single molecules with ultra-high precision, which is suitable for tracking in living cells. Numerical simulations were conducted to explore the performance and potential applications. The results confirm that the proposed method can provide reasonable estimates even under weak signal conditions.
Article
Biology
Ha H. Park, Bowen Wang, Suhong Moon, Tyler Jepson, Ke Xu
Summary: This study presents a machine-learning-enabled approach called Pix2D, which extracts the diffusion coefficient D directly from single molecule images and enables high-resolution spatial mapping of D.
COMMUNICATIONS BIOLOGY
(2023)
Article
Biochemical Research Methods
John S. H. Danial, Jeff Y. L. Lam, Yunzhao Wu, Matthew Woolley, Eleni Dimou, Matthew R. Cheetham, Derya Emin, David Klenerman
Summary: This article presents an economic and easy-to-assemble single-molecule localization microscopy setup called NanoPro for high-throughput super-resolution imaging. It provides step-by-step instructions and illustrations to aid researchers in constructing and utilizing NanoPro, as well as assessing its performance.
Article
Multidisciplinary Sciences
Filipe Nunes Vicente, Mickael Lelek, Jean-Yves Tinevez, Quang D. Tran, Gerard Pehau-Arnaudet, Christophe Zimmer, Sandrine Etienne-Manneville, Gregory Giannone, Cecile Leduc
Summary: Intermediate filaments play key roles in cellular functions and their mechanical properties. This study used super-resolution microscopy to reveal the molecular organization of vimentin filaments and provided evidence for the partial overlap of subunits during filament assembly. The researchers also found that the extensibility of vimentin is due to the unfolding of its subunits, establishing a direct link between structure and mechanics.
Article
Optics
Mengchao Ma, Yi Zhang, Huaxia Deng, Xicheng Gao, Lei Gu, Qianzhen Sun, Yilong Su, Xiang Zhong
Summary: By introducing the single-pixel superposition compound eye (SPSCE), we have successfully improved the imaging resolution, making it more reliable than traditional compound eyes with super-high imaging capabilities, able to capture the entire object image.
OPTICS AND LASERS IN ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Wei Lin, Gary C. H. Mo, Sohum Mehta, Jin Zhang
Summary: Super-resolution activity imaging currently does not consider the locations of collaborating regulators/effectors in living cells. The DrFLINC technique, based on the FLINC principle, utilizes nonfluorescent Dronpa to enhance fluorescence fluctuations, leading to the development of a superior red label and a next-generation activity sensor for context-rich super-resolution biosensing.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Michael Holtmannspoetter, Eike Wienbeuker, Timo Dellmann, Isabelle Watrinet, Ana J. Garcia-Saez, Kai Johnsson, Rainer Kurre, Jacob Piehler
Summary: Self-labeling enzymes (SLE) like the HaloTag are valuable tools in high and super-resolution fluorescence microscopy. New fluorogenic substrates for SLE allow imaging with excess dye. Two engineered variants of HaloTag7 with restored dehalogenase activity, reHaloTagS (approx. 0.006 s(-1)) and reHaloTagF (approx. 0.055 s(-1)), showed different turnover kinetics in vitro. Imaging with reHaloTag labeling demonstrated 3-5 times enhanced photostability, and single molecule imaging enabled controlled and stable labeling density over extended periods. Combination with structured illumination allowed simultaneous visualization of single molecule diffusion and organellar dynamics. These applications showcase the potential of reHaloTag labeling in advancing fluorescence microscopy techniques.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Optics
Nisan Siegel, Gary Brooker
Summary: This new super-resolution microscopy technique utilizes a simple and versatile approach to achieve better resolution than other complex methods after a single image capture. It can work with any objective power and numerical aperture, is easy to operate and maintain, and can create super-resolved images using any type or wavelength of light.
Article
Cell Biology
James H. Felce, Lucia Parolini, Erdinc Sezgin, Pablo F. Cespedes, Kseniya Korobchevskaya, Mathew Jones, Yanchun Peng, Tao Dong, Marco Fritzsche, Dirk Aarts, John Frater, Michael L. Dustin
Summary: The study reveals the spatiotemporal organization of GPCRs within the synapse of T cells, focusing on the contribution of CXCR4 in T cell activation. These GPCRs show a similar pattern of organization in the synapse to CXCR4, providing insights into how they contribute to T cell activation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Optics
Haitang Yang, Esther Y. H. Lin, Kiriakos N. Kutulakos, George V. Eleftheriades
Summary: Super-oscillatory imaging is a technique that can go beyond the diffraction limit. This study provides a comprehensive theoretical analysis and experimental validation of the integration of super-oscillatory imaging into existing microscopes and telescopes, as well as the operational limits of the approach.
Article
Chemistry, Multidisciplinary
Anish R. Roy, Wei Zhang, Zeinab Jahed, Ching-Ting Tsai, Bianxiao Cui, W. E. Moerner
Summary: The topography of the plasma membrane has a strong influence on cellular processes. Recent studies have used 3D nanostructures to study the effects of membrane curvature on protein distribution. In this study, a method combining silicone-oil immersion objective and 3D double-helix point spread function microscopy is introduced to investigate the nanoscale distribution of proteins at the nano-bio interface.
Article
Cell Biology
Lianguang Shang, Xiaoxia Li, Huiying He, Qiaoling Yuan, Yanni Song, Zhaoran Wei, Hai Lin, Min Hu, Fengli Zhao, Chao Zhang, Yuhua Li, Hongsheng Gao, Tianyi Wang, Xiangpei Liu, Hong Zhang, Ya Zhang, Shuaimin Cao, Xiaoman Yu, Bintao Zhang, Yong Zhang, Yiqing Tan, Mao Qin, Cheng Ai, Yingxue Yang, Bin Zhang, Zhiqiang Hu, Hongru Wang, Yang Lv, Yuexing Wang, Jie Ma, Quan Wang, Hongwei Lu, Zhe Wu, Shanlin Liu, Zongyi Sun, Hongliang Zhang, Longbiao Guo, Zichao Li, Yongfeng Zhou, Jiayang Li, Zuofeng Zhu, Guosheng Xiong, Jue Ruan, Qian Qian
Summary: By studying the pan-genomes of large natural populations, we can uncover the genetic diversity and complexity of rice. We have created a super pan-genome of rice using long-read assembly and identified extensive structural variations and gene presence/absence variations. Additionally, we have discovered genetic variants associated with various traits in rice and identified a common set of genes that have played a role in adaptation and domestication in Asian and African rice.
Article
Computer Science, Artificial Intelligence
Muhammad Haris, Greg Shakhnarovich, Norimichi Ukita
Summary: Deep Back-Projection Networks (DBPN) utilize iterative up- and down-sampling layers to construct mutually-connected units, providing an error feedback mechanism and addressing the mutual dependencies between low- and high-resolution images. By extending this idea with parameter sharing and transition layers, DBPN achieves superior results across multiple data sets, particularly for large scaling factors like 8x.
IEEE TRANSACTIONS ON PATTERN ANALYSIS AND MACHINE INTELLIGENCE
(2021)
Review
Oncology
Thomas Orre, Olivier Rossier, Gregory Giannone
EXPERIMENTAL CELL RESEARCH
(2019)
Article
Biochemical Research Methods
V. V. G. Krishna Inavalli, Martin O. Lenz, Corey Butler, Julie Angibaud, Benjamin Compans, Florian Levet, Jan Tonnesen, Olivier Rossier, Gregory Giannone, Olivier Thoumine, Eric Hosy, Daniel Choquet, Jean-Baptiste Sibarita, U. Valentin Nagerl
Article
Cell Biology
Sophie Massou, Filipe Nunes Vicente, Franziska Wetzel, Amine Mehidi, Dan Strehle, Cecile Leduc, Raphael Voituriez, Olivier Rossier, Pierre Nassoy, Gregory Giannone
NATURE CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mariem Souissi, Julien Pernier, Olivier Rossier, Gregory Giannone, Christophe Le Clainche, Emmanuele Helfer, Kheya Sengupta
Summary: This study describes a new method for preparing GUVs incorporating the transmembrane protein integrin, with successful incorporation of functional integrin molecules.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Thomas Orre, Adrien Joly, Zeynep Karatas, Birgit Kastberger, Clement Cabriel, Ralph T. Bottcher, Sandrine Leveque-Fort, Jean-Baptiste Sibarita, Reinhard Fassler, Bernhard Wehrle-Haller, Olivier Rossier, Gregory Giannone
Summary: Focal adhesions (FAs) play a key role in initiating chemical and mechanical signals for cell polarity, migration, proliferation, and differentiation. The study integrates single protein tracking, super-resolution microscopy, and functional assays to correlate the molecular behavior and 3D nanoscale localization of kindlin with its function in integrin activation inside FAs. This research provides insights into the molecular mechanisms underlying the interaction of kindlin with integrins in focal adhesions.
NATURE COMMUNICATIONS
(2021)
Editorial Material
Medicine, Research & Experimental
Filipe Nunes Vicente, Olivier Rossier, Gregory Giannone
M S-MEDECINE SCIENCES
(2022)
Article
Cell Biology
Amine Mehidi, Frieda Kage, Zeynep Karatas, Maureen Cercy, Matthias Schaks, Anna Polesskaya, Matthieu Sainlos, Alexis M. Gautreau, Olivier Rossier, Klemens Rottner, Gregory Giannone
Summary: Mehidi et al. demonstrate that piconewton forces exerted by the polymerization of individual actin filaments displace the WAVE regulatory complex from lamellipodial tips, thereby regulating WAVE complex activity during cell migration.
NATURE CELL BIOLOGY
(2021)
Article
Cell Biology
Amaris Guevara-Garcia, Laure Fourel, Ingrid Bourrin-Reynard, Adria Sales, Christiane Oddou, Mylene Pezet, Olivier Rossier, Paul Machillot, Line Chaar, Anne-Pascale Bouin, Gregory Giannone, Olivier Destaing, Catherine Picart, Corinne Albiges-Rizo
Summary: This article investigates the decoupling of functions between ALK3 and BMPRII, and demonstrates that BMP2 stimulation controls the spatial organization of BMPRs by segregating ALK3 from BMPRII and recruiting it to beta 3 integrin-containing focal adhesions. The findings suggest that the spatial control of ALK3 in focal adhesions is crucial for cell adhesion processes and the stability of SMAD. The study sheds light on the interplay between integrins and BMP receptors in guiding adhesive processes.
JOURNAL OF CELL BIOLOGY
(2022)
Review
Cell Biology
Filipe Nunes Vicente, Tianchi Chen, Olivier Rossier, Gregory Giannone
Summary: Detection and conversion of mechanical forces into biochemical signals, known as mechanotransduction, play vital roles in regulating various cellular processes. Recent advancements in super-resolution microscopy and molecular force sensors have revolutionized the understanding of molecular mechanotransduction in live cells, particularly in integrin adhesions, actin structures, and the plasma membrane.
TRENDS IN CELL BIOLOGY
(2023)
Article
Cell Biology
Carla Silva Martins, Cyntia Taveneau, Gerard Castro-Linares, Mikhail Baibakov, Nicolas Buzhinsky, Mar Eroles, Violeta Milanovic, Shizue Omi, Jean-Denis Pedelacq, Francois Iv, Lea Bouillard, Alexander Llewellyn, Maxime Gomes, Mayssa Belhabib, Mira Kuzmic, Pascal Verdier-Pinard, Stacey Lee, Ali Badache, Sanjay Kumar, Cristel Chandre, Sophie Brasselet, Felix Rico, Olivier Rossier, Gijsje H. Koenderink, Jerome Wenger, Stephanie Cabantous, Manos Mavrakis
Summary: Septins are cytoskeletal proteins that form octamer-containing filaments and mediate actin-membrane anchoring, contributing to cell stiffness and mechanics.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Julien Pernier, Marcelina Cardoso Dos Santos, Mariem Souissi, Adrien Joly, Hemalatha Narassimprakash, Olivier Rossier, Gregory Giannone, Emmanuele Helfer, Kheya Sengupta, Christophe Le Clainche
Summary: Focal adhesions are essential for cell adhesion, in which talin and kindlin play important roles. This study demonstrates that talin and kindlin can individually cluster integrins and work together to form larger integrin clusters. The findings provide insights into the mechanism of integrin clustering.
JOURNAL OF CELL SCIENCE
(2023)