Article
Cardiac & Cardiovascular Systems
Hidenori Tani, Taketaro Sadahiro, Yu Yamada, Mari Isomi, Hiroyuki Yamakawa, Ryo Fujita, Yuto Abe, Tatsuya Akiyama, Koji Nakano, Yuta Kuze, Masahide Seki, Yutaka Suzuki, Manabu Fujisawa, Mamiko Sakata-Yanagimoto, Shigeru Chiba, Keiichi Fukuda, Masaki Ieda
Summary: By conducting experiments on mice, this study found that cardiac reprogramming can repair chronic myocardial infarction, promote myocardial regeneration, and reduce fibrosis. The results of this study provide new insights for the treatment of chronic myocardial infarction and heart failure.
Article
Cardiac & Cardiovascular Systems
Hidenori Tani, Taketaro Sadahiro, Yu Yamada, Mari Isomi, Hiroyuki Yamakawa, Ryo Fujita, Yuto Abe, Tatsuya Akiyama, Koji Nakano, Yuta Kuze, Masahide Seki, Yutaka Suzuki, Manabu Fujisawa, Mamiko Sakata-Yanagimoto, Shigeru Chiba, Keiichi Fukuda, Masaki Ieda
Summary: Adult cardiomyocytes have limited regenerative ability, and the synthesis of extracellular matrix by cardiac fibroblasts (CFs) results in fibrosis and heart dysfunction after myocardial infarction (MI). The overexpression of cardiac transcription factors, MGTH, can directly reprogram CFs into induced cardiomyocytes (iCMs) and improve cardiac function in acute MI. However, it is unclear whether in vivo cardiac reprogramming can repair chronic MI with established scars.
Article
Multidisciplinary Sciences
Hidefumi Iwashita, Erika Castillo, Marco S. Messina, Raymond A. Swanson, Christopher J. Chang
Summary: A new tandem activity-based sensing and labeling strategy for H2O2 imaging has been reported, allowing capture and permanent recording of localized H2O2 fluxes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Hidefumi Iwashita, Erika Castillo, Marco S. Messina, Raymond A. Swanson, Christopher J. Chang
Summary: This study presents an advanced strategy for imaging H2O2 using a tandem activity-based sensing and labeling approach, allowing capture and recording of localized H2O2 fluxes. It enables visualization of transcellular redox signaling and provides a starting point for designing chemical probes with high spatial fidelity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Thuan Thai, Fei Zhong, Lei Dang, Enoch Chan, Jacqueline Ku, Ernst Malle, Carolyn L. Geczy, John F. Keaney, Shane R. Thomas
Summary: The study demonstrates that transcytosed MPO in endothelial cells activates eNOS by promoting phospholipase C-dependent calcium signaling and altering eNOS phosphorylation status. This activation occurs despite reduced cellular uptake of the eNOS substrate L-arginine. These findings suggest a compensatory signaling response in endothelial cells to maintain eNOS activity and NO production in the presence of MPO-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Chemistry, Multidisciplinary
Wenxin Yao, Yanjun Cao, Mengyao She, Yuanyuan Yan, Jinxin Li, Xin Leng, Ping Liu, Shengyong Zhang, Jianli Li
Summary: Heart failure is considered to be closely related to oxidative stress, and a novel fluorescent probe BCO has been designed to evaluate cellular oxidative stress levels, providing a useful tool for further understanding the pathophysiology process of heart disease.
Review
Biochemistry & Molecular Biology
Helmut Sies
Summary: The spatiotemporal control of redox signaling in cells relies on continuous fine-tuning, with molecules like H2O2 playing a key role. Oxidative eustress operates within a specific physiological range, and is managed through both short-term and long-term homeostatic mechanisms to respond to stress.
Article
Cardiac & Cardiovascular Systems
Njabulo Ngwenyama, Annet Kirabo, Mark Aronovitz, Francisco Velazquez, Francisco Carrillo-Salinas, Ane M. Salvador, Tania Nevers, Venkataraman Amarnath, Albert Tai, Robert M. Blanton, David G. Harrison, Pilar Alcaide
Summary: The study found that TCR antigen recognition increases in the left ventricle as cardiac dysfunction progresses, with a limited repertoire of activated CD4+ T-cell clonotypes identified in the left ventricle. Antigen presentation of endogenous antigens was necessary for the development of cardiac dysfunction, and scavenging IsoLGs reduced TCR activation and prevented cardiac dysfunction. This highlights the important role of reactive oxygen species-induced formation of IsoLG-modified cardiac neoantigens in TCR-dependent CD4+ T-cell activation within the heart.
Article
Biochemistry & Molecular Biology
Andreas Daiber, Ioanna Andreadou, Matthias Oelze, Sean M. Davidson, Derek J. Hausenloy
Summary: Global epidemiological studies have shown a shift towards chronic non-communicable diseases such as atherosclerosis and metabolic disorders, with ischemic heart disease being a leading cause of mortality and morbidity affecting 128 million people worldwide. Reactive oxygen species play a central role in ischemia/reperfusion damage but also have beneficial effects in cellular signaling, making redox therapy complex and challenging.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Elzbieta Lazar-Poloczek, Ewa Romuk, Piotr Rozentryt, Sylwia Duda, Mariusz Gasior, Celina Wojciechowska
Summary: This study found a correlation between ceruloplasmin levels and various biomarkers in patients with HFrEF, suggesting ceruloplasmin's potential as a useful biomarker in this population. Ceruloplasmin was positively correlated with cardiac and hepatic status, inflammatory markers, and oxidative stress indicators. These results provide new insights for classification and treatment strategies for HFrEF patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Markus Waldeck-Weiermair, Shambhu Yadav, Fotios Spyropoulos, Christina Kruger, Arvind K. Pandey, Thomas Michel
Summary: Hydrogen peroxide (H2O2) serves as a versatile cell signaling molecule at low concentrations, but can be toxic at higher concentrations by promoting oxidative stress. The differential distribution of H2O2 in cells and the varied distribution of redox-active enzymes play crucial roles in cellular functions. Chemogenetic approaches utilizing D-amino acid oxidase (DAAO) have been developed to dynamically regulate H2O2 levels in specific subcellular locations, offering insights into the physiological and pathological oxidative pathways.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
David Weissman, Christoph Maack
Summary: Heart failure is a growing health burden globally, characterized by alterations in excitation-contraction coupling, cardiac energy deficit, and oxidative stress. While targeting metabolism may provide prognostic benefits, treatments specifically targeting reactive oxygen species (ROS) are still in preclinical stages. More research is needed in this area to develop effective therapies for heart failure.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Hualing Sun, Richard E. Pratt, Victor J. Dzau, Conrad P. Hodgkinson
Summary: Directly reprogramming fibroblasts into cardiomyocytes can improve cardiac function in the infarcted heart, but the low efficacy of this approach limits its clinical applications. We hypothesized that birth imposes fundamental changes in cardiac fibroblasts, thereby affecting their regenerative capabilities. Our study found that fibroblasts derived from neonatal mice showed higher reprogramming efficacy compared to those derived from adult mice, possibly due to the differential expression of pro-angiogenic genes and the transcription factor Epas1. Targeted knockdown of Epas1 improved reprogramming efficacy in adult cardiac fibroblasts, and the addition of an Epas1 targeting siRNA significantly enhanced reprogramming efficacy in vivo.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Toshihide Kashihara, Risa Mukai, Shin-ichi Oka, Peiyong Zhai, Yasuki Nakada, Zhi Yang, Wataru Mizushima, Tsutomu Nakahara, Junco S. Warren, Maha Abdellatif, Junichi Sadoshima
Summary: The heart utilizes multiple adaptive mechanisms to maintain pump function, with YAP playing a crucial role in inducing compensatory cardiac hypertrophy through promoting glycolysis and upregulating the GLUT1 gene. This leads to the accumulation of glycolytic metabolites and ultimately promotes cardiac hypertrophy.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biochemistry & Molecular Biology
Sergio Rius-Perez
Summary: p53 is a redox-sensitive transcription factor that can regulate multiple cell death programs, including necroptosis, through various signaling pathways. It plays a crucial role in modulating necroptosis execution by regulating mitochondrial reactive oxygen species (mtROS) levels and interacting with key components of the necrosome. p53 controls the generation of mtROS and upregulates the translation of necroptosis-related factors, such as RIPK1 and RIPK3, in response to increased levels of H2O2. Additionally, p53 migration into mitochondria contributes to necroptosis execution via interaction with the mitochondrial permeability transition pore.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)