Article
Multidisciplinary Sciences
Xiaolu Zhang, Gulshan R. Walke, Istvan Horvath, Ranjeet Kumar, Stephanie Blockhuys, Stellan Holgersson, Paul H. Walton, Pernilla Wittung-Stafshede
Summary: Memo1 is a protein associated with cancer that binds copper ions and protects them from reactive oxygen species generation. It interacts with the cytoplasmic copper chaperone Atox1 and exchanges copper ions. This functionality may be a safety mechanism to cope with the increased demand of copper ions in cancer cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Renana Schwartz, Sharon Ruthstein, Dan Thomas Major
Summary: This study analyzes the impact of copper ion coordination states on protein flexibility in the metallochaperone Atox1 and proposes a mechanism hypothesis for copper ion transmission.
Article
Biochemistry & Molecular Biology
Mao Kondo, Hirokazu Hara, Fuka Kamijo, Tetsuro Kamiya, Tetsuo Adachi
Summary: In this study, the neurotoxin 6-OHDA was found to reduce the protein levels of the Cu exporter ATP7A and the Cu chaperone Atox1 by promoting oxidative stress-induced degradation. Cells pretreated with 6-OHDA showed significantly higher intracellular Cu levels after exposure to CuCl2, and 6-OHDA also decreased the protein levels of the cuproenzyme dopamine beta-hydroxylase. This suggests that 6-OHDA disrupts Cu homeostasis through dysregulation of cellular Cu trafficking, leading to neuronal cell dysfunction.
Article
Anatomy & Morphology
Rehman Ullah, Safia Sher, Zahir Muhammad, Saiqa Afriq Jan, Muhammad Nafees
Summary: The study aimed to modulate the physiological response of sunflowers in a stressed environment using Urea capped hydroxyapatite nanoparticles (Urea-HANPs). Results revealed that Urea-HANPs significantly improved the plant's resistivity to osmotic stress and enhanced agronomic and physiobiochemical attributes.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Biochemistry & Molecular Biology
Anna Caccamo, Felix Vega de Luna, Khadija Wahni, Alexander N. Volkov, Jonathan Przybyla-Toscano, Antonello Amelii, Alexandre Kriznik, Nicolas Rouhier, Joris Messens, Claire Remacle
Summary: Recent studies have identified a novel class of ascorbate peroxidases called APX-R, with a focus on APX2 from Chlamydomonas reinhardtii in this study. Experimental results indicate that APX2 exhibits peroxidase activity with guaiacol as an electron donor, along with the ability to bind copper and heme. Evidence suggests a potential interaction between APX2 and plastocyanin, highlighting the role of APX2 in regulating copper transfer to plastocyanin.
Article
Biology
Sandeep Kumar, Mohammad Abass Ahanger, Huda Alshaya, Basit Latief Jan, Vimala Yerramilli
Summary: The study demonstrates the positive effect of salicylic acid treatment on the growth and yield of Capsicum annuum L. plants under salt stress, particularly during the flowering stage. Additionally, salicylic acid promotes proline accumulation, enhancing salt stress tolerance in the plants.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Letter
Plant Sciences
Yosef Fichman, Ron Mittler
Summary: The wounding-induced reactive oxygen species (ROS) wave triggers a systemic redox response throughout the whole plant in Arabidopsis.
Article
Microbiology
Hui Li, Cheng-Wen He, Jing Zhu, Zhiping Xie
Summary: Yeast cells are a valuable model system for studying cellular processes, but their small size makes it challenging to observe and recognize subcellular structures. In this study, researchers developed organelle markers for yeast cells to be used in electron microscopy. They used genetically encoded tags to specifically label organelles and verified their localization.
Review
Biochemistry & Molecular Biology
Ronja Weissinger, Lisa Heinold, Saira Akram, Ralf-Peter Jansen, Orit Hermesh
Summary: The interaction between RNAs and proteins controls multiple cellular functions and specific knowledge is required to understand their interactions. Various techniques have been developed to study RNA-protein interactions including CLIP and RNA affinity purification. In vivo proximity labeling can be used to study RNA-protein interactions and analyze tagged molecules via mass spectrometry or RNA-Seq.
Article
Agriculture, Multidisciplinary
Wang Ya-di, Li Fei, Zhang Xin, Liu Ting-li, Liang Wen-xing, Li De-long
Summary: In this study, a small cysteine-rich (SCR) effector protein, PnSCR82, was identified in Phytophthora nicotianae. It was demonstrated that PnSCR82 induced hypersensitive cell death in Nicotiana benthamiana and Solanum lycopersicum, and enhanced plant resistance to Phytophthora capsici. Real-time PCR results showed that PnSCR82 activated multiple signaling pathways. These findings suggest that PnSCR82 elicits defensive responses and may have a significant role in crop protection programs.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Plant Sciences
Chunqing Wang, Qi Zhang, Jiaxin Li, Xinliang Wang, Chuxin Li, Yu Bin, Zhen Song
Summary: This study reveals that the coat protein of Citrus yellow vein clearing virus (CYVCV) interacts directly with the ascorbate peroxidase 1 of lemon (ClAPX1). The findings suggest that the virus can regulate the antiviral immune response of lemon by manipulating the activity of ClAPX1, possibly through the jasmonic acid signaling pathway.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Myeong-Gyun Kang, Hyun-Woo Rhee
Summary: Proximity labeling is an enzymatic chemical reaction that modifies biomolecules in live cells based on their proximity. It has been used to characterize local proteomes and spatiotemporal interactome information in live cells. The method can also be applied for profiling local RNA and DNA and is promising for studying spatial cell-cell interaction networks in live animal models.
ACCOUNTS OF CHEMICAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Arjan de Groot, Laurence Blanchard, Nicolas Rouhier, Pascal Rey
Summary: Deinococcus species have remarkable tolerance to oxidative damage in extreme environmental conditions. Thiol reductases (TRs) play important roles in metabolism regulation and stress responses. The study reveals that Deinococcus possesses multiple enzyme systems that protect proteins and maintain redox status. Recent research further confirms the importance of TRs in Deinococcus' tolerance to oxidative treatments.
Article
Chemistry, Multidisciplinary
Li Lei, Zhifeng Zou, Jin Liu, Zhiai Xu, Ying Fu, Yang Tian, Wen Zhang
Summary: The excessive production and deposition of A beta is a key factor in Alzheimer's disease, and targeting oxidative stress and reducing A beta plaques may be a promising approach for treatment. The design of multifunctional micelles to clear A beta and ROS simultaneously shows potential for novel AD therapy.
Article
Chemistry, Multidisciplinary
Michael Okafor, Paulina Gonzalez, Pascale Ronot, Islah El Masoudi, Anne Boos, Stephane Ory, Sylvette Chasserot-Golaz, Stephane Gasman, Laurent Raibaut, Christelle Hureau, Nicolas Vitale, Peter Faller
Summary: Copper homeostasis is crucial for mammal's health and its dysregulation is implicated in diseases like Alzheimer's. In this study, a novel peptide-based copper shuttle is designed, which can selectively transport copper ions into cells and remove copper from amyloid plaques, inhibiting oxidative stress and cell toxicity. This mechanism shows potential applications in treating copper-related diseases.
Article
Chemistry, Medicinal
Ke Huang, Lulu Jiang, Ronghui Liang, Huiti Li, Xiaoxue Ruan, Changliang Shan, Deyong Ye, Lu Zhou
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Article
Pharmacology & Pharmacy
Yanping Li, Ronghui Liang, Xiaoya Zhang, Jiyan Wang, Changliang Shan, Shuangping Liu, Leilei Li, Shuai Zhang
FRONTIERS IN PHARMACOLOGY
(2019)
Article
Pathology
Xuanyu Wang, Yongjun Shang, Lina Yang, Xiaoyu Tan, Huamin Zhang, Changliang Shan, Shuangping Liu
PATHOLOGY RESEARCH AND PRACTICE
(2019)
Article
Chemistry, Medicinal
Zhongyuan Luo, Daohai Du, Yanjun Liu, Tian Lu, Liping Liu, Hualiang Jiang, Kaixian Chen, Changliang Shan, Cheng Luo
Summary: A new high-throughput screening method was developed to discover G6PD inhibitors, with Wedelolactone identified as a strong inhibitor in a non-competitive, reversible manner, as confirmed by surface Plasmon Resonance assay. The study also showed the inhibitory effect of Wedelolactone on ovarian cancer cell proliferation through targeting G6PD.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Medicine, General & Internal
Yu Yuan, Chenxin Yang, Yingzhi Wang, Mingming Sun, Chenghao Bi, Sitong Sun, Guijiang Sun, Jingpeng Hao, Lingling Li, Changliang Shan, Shuai Zhang, Yubo Li
Summary: This study aimed to compare the metabolic differences between CRC patients and healthy controls and identify potential biomarkers for early diagnosis and targeted therapy. The findings revealed that glycodeoxycholic acid (GDCA) in serum was positively correlated with CRC and could serve as a molecular biomarker. In vitro experiments showed that GDCA promoted the proliferation and migration of CRC cells, and it was identified that GDCA targeted Poly(ADP-ribose) polymerase-1 (PARP-1).
Article
Biochemistry & Molecular Biology
Jiyan Wang, Yaya Qiao, Huanran Sun, Hongkai Chang, Huifang Zhao, Shuai Zhang, Changliang Shan
Summary: Tyrosine is an essential amino acid involved in the metabolism of proteins, lipids, and carbohydrates. Down-regulation of tyrosine-metabolizing enzymes in hepatocellular carcinoma (HCC) is associated with a poor prognosis. Reduced tyrosine metabolism activates the cell cycle and promotes cell proliferation.
Review
Biology
Jiaqi Song, Huanran Sun, Shuai Zhang, Changliang Shan
Summary: The pentose phosphate pathway (PPP) is an important metabolic pathway that provides crucial substances such as NADPH and nucleotides for cellular activities. G6PD, the rate-limiting enzyme of PPP, is upregulated in various cancers and its dysfunction influences cancer cell growth, invasion, and chemotherapeutic resistance. Targeting G6PD has shown promise as a strategy in treating cancer and reversing chemoresistance.
Article
Pharmacology & Pharmacy
Jianshuang Guo, Kaihui Liu, Jiyan Wang, Hao Jiang, Mengyi Zhang, Yang Liu, Changliang Shan, Fangzhong Hu, Wenzheng Fu, Chunze Zhang, Jing Li, Yue Chen
Summary: This study reveals the mechanism of MCL-induced oxidative stress in leukemia and glioblastoma cells, involving the mediation of reduced glutathione (GSH) and impairment of mitochondrial function. Furthermore, the inhibition of GSH biosynthesis by BSO enhances the therapeutic effect of MCL by suppressing GSH compensatory mechanism and promoting cancer cell oxidative stress.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jiyan Wang, Hongkai Chang, Meng Su, Yaya Qiao, Huanran Sun, Yongshan Zhao, Shuai Zhang, Changliang Shan
Summary: Kidney renal clear cell carcinoma (KIRC) is the most common subtype of renal cell carcinoma, with a poor prognosis. In this study, we identified the biomarkers HGD and GSTZ1 related to the prognosis of KIRC through bioinformatics analysis. We found that they regulate the metabolism and energy balance of tumor cells, promoting tumor progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Mingming Sun, Leilei Li, Yujia Niu, Yingzhi Wang, Qi Yan, Fei Xie, Yaya Qiao, Jiaqi Song, Huanran Sun, Zhen Li, Sizhen Lai, Hongkai Chang, Han Zhang, Jiyan Wang, Chenxin Yang, Huifang Zhao, Junzhen Tan, Yanping Li, Shuangping Liu, Bin Lu, Min Liu, Guangyao Kong, Yujun Zhao, Chunze Zhang, Shu-Hai Lin, Cheng Luo, Shuai Zhang, Changliang Shan
Summary: PRMT6 is highly expressed in lung cancer and regulates glucose metabolism. It methylates 6PGD and ENO1 to enhance their activities, affecting the oxidative PPP flux and glycolysis pathway. Targeting PRMT6 inhibits tumor growth, blocks glucose metabolism, and enhances the anti-tumor effects of cisplatin.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Oncology
Jiyan Wang, Yaya Qiao, Mingming Sun, Huanran Sun, Fei Xie, Hongkai Chang, Yingzhi Wang, Jiaqi Song, Sizhen Lai, Chenxin Yang, Xichuan Li, Shuangping Liu, Xuanzhu Zhao, Kemin Ni, Kewei Meng, Shuai Zhang, Changliang Shan, Chunze Zhang
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Oncology
Taoyuan Wang, Tiansheng Tang, Youguo Jiang, Tao He, Luyu Qi, Hongkai Chang, Yaya Qiao, Mingming Sun, Changliang Shan, Xinyuan Zhu, Jianshi Liu, Jiyan Wang
Summary: p53 mutations are common in human cancer, and in this study, it was found that elevated expression of PRIM2 in p53-mutated lung cancer is associated with tumor progression. The expression of PRIM2 is regulated by p53 and serves as a biomarker for lung cancer malignancy and survival prognosis.
Article
Biochemistry & Molecular Biology
Mingming Sun, Qi Yan, Yaya Qiao, Huifang Zhao, Yingzhi Wang, Changliang Shan, Shuai Zhang
Summary: PIKE-A promotes cancer cell proliferation by regulating mitochondrial membrane potential through the STAT3/FTO/SDHA axis, providing a novel target for anti-cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Editorial Material
Biochemistry & Molecular Biology
Jiyan Wang, Xintong Dai, Hongkai Chang, Qingle Gao, Jianshuang Guo, Juze Yang, Shuai Zhang, Changliang Shan