Article
Biochemical Research Methods
Dongwei Wang, Jiasheng Wang, Zhiling Song, Ni Hui
Summary: Ultrasensitive and low-fouling microRNA electrochemical biosensors were constructed by introducing thiol-terminated antifouling molecules onto polyaniline-modified electrodes. Among the investigated antifouling materials, a newly designed and synthesized peptide showed superior antifouling ability and highest specificity for various microRNA targets. The introduction of antifouling molecules did not compromise the sensitivity of the biosensor, indicating promising applications in developing biofouling-resistant sensors and biosensors.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Juan Chen, Mingyue Xie, Mingqing Shi, Kun Yuan, Yanan Wu, Hong-Min Meng, Lingbo Qu, Zhaohui Li
Summary: In this study, an accelerated DNA nanoprobe was developed for fast, in situ monitoring of miRNA in exosomes. The nanoprobe not only detects miRNA, but also distinguishes tumor exosomes from those derived from normal cells, and can be used to study exosome biological functions.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Loredana Mereuta, Alina Asandei, Irina Schiopu, Jonggwan Park, Yoonkyung Park, Tudor Luchian
Summary: Nanopores offer sensitive and cost-effective single-molecule sensing capabilities, and the advent of nanopore-based DNA detection and sequencing technologies has had a significant societal impact. To address the challenge of forming stable, long-lasting single nanopores, we have developed a new approach for detecting small single-stranded DNA using dynamic hybrid constructs.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Yueh-Er Chiou, Kai-Jie Yu, Sow-Neng Pang, Yan-Lin Yang, See-Tong Pang, Wen-Hui Weng
Summary: The study successfully detected exomiR-451 and exomiR-21 in the urine of prostate cancer patients using a SPCE-based biosensor, confirming their diagnostic significance. The measurement of CV and PAS values can be used to identify the presence of disease and further validate the results. Additionally, exomiR-21 is associated with tumor progression, making the biosensor crucial for prostate cancer screening.
BIOENGINEERING-BASEL
(2022)
Article
Chemistry, Analytical
Tingxian Li, Lina Tang, Kun Li, Binzhu Liu, Meng -Meng Xiao, Nian Liu, Wei Ni, Yutao Li, Zhiyong Zhang, Guo-Jun Zhang
Summary: To achieve fast and sensitive detection of proteins, researchers have developed a polymer-sorted high-purity semiconducting carbon nanotubes (CNTs) films-based field-effect transistor (FET) biosensor for ultrasensitive and label-free detection of MUC1, a transmembrane protein highly expressed in breast cancer exosomes. The modified CNT FET was capable of sensitively and selectively detecting exosomal MUC1 as low as 0.34 fg/mL, and it could differentiate breast cancer patients from healthy individuals by comparing the expression level of exosomal MUC1. This novel CNT FET biosensor holds great potential for early diagnosis of cancer.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Ye Zhang, Xiaohe Zhang, Bo Situ, Yuan Wu, Shihua Luo, Lei Zheng, Yurong Qiu
Summary: The study introduced a robust electrochemical biosensor using multifunctional DNA tetrahedrons for exo-miRNA analysis, demonstrating high sensitivity and rapid detection. The platform showed efficient quantification of exo-miRNA and profiling of tumor-associated miRNAs in breast cancer, with high sensitivity for breast tumor diagnosis and early detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Dongwei Wang, Jiasheng Wang
Summary: The study demonstrated the potential of functionalized polypyrrole nanowires as a platform for label-free miRNA detection using EIS, and constructed a sensitive and selective miRNA biosensor based on PPyNWs/PAMAM hybrid. The biosensor showed excellent sensing performances in monitoring DNA/miRNA hybridization, indicating the microstructure of the PPy nanowires array greatly improved its performance.
Article
Chemistry, Multidisciplinary
Satish Kasturi, Yunji Eom, Sri Ramulu Torati, CheolGi Kim
Summary: A novel high-resolution electrochemical biosensor based on rGO/Au was developed for the detection of miRNA-122, showing superior analytical performance and stability. The sensor demonstrated linear response to various concentrations of miRNA-122 by anchoring probe DNA and recognizing the target molecule.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qianrui Liu, Huifang Xie, Jinming Kong, Xueji Zhang
Summary: An original electrochemical biosensor using Cu-mediated near-infrared light polymerization was developed for highly sensitive nucleic acid detection. This biosensor showed portable and cost-efficient features and has great potential in biological detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biophysics
Tao Li, Rui Hu, Jianbo Xia, Zhichen Xu, Dongjuan Chen, Jinou Xi, Bi-Feng Liu, Jiang Zhu, Ying Li, Yunhuang Yang, Maili Liu
Summary: By utilizing G-triplex (G3) as a reporter gene, G-CRISPR achieves a 20-fold improvement in analytical sensitivity, enabling detection of unamplified and amplified DNA as low as 50 pmol and 0.1 amol (one copy/reaction) respectively. The results demonstrate high specificity and sensitivity, and suggest that G-CRISPR could serve as a rapid, sensitive, and reliable biosensor in biomedical diagnostics.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Qingyu Wang, Jingliang Liu, Shuaibing Yu, Haobo Sun, Lei Wang, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: A peptide-based biosensor was developed for sensitive and selective analysis of matrix metalloproteinase 2 (MMP-2) using a signal amplification strategy. The biosensor showed good performance in determining MMP-2 in human serum samples and has great potential in clinical analysis applications.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Jie Bai, Eryi Shu, Tao Zhang, Hongliang Fan
Summary: MicroRNAs (miRNAs) are small RNA molecules that can be used as diagnostic/prognostic biomarkers and therapeutic targets for diseases. This study proposes an identification-cleavage-amplification (ICA) strategy for highly sensitive and versatile detection of miRNA, successfully applied to miR-155 and miR-21 assays. The strategy combines aligner-target mediated cleavage with strand displacement amplification (ATMC-SDA) for the ICA process.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Yujuan Qiao, Jing-Jing Hu, Yuxin Hu, Chong Duan, Wenlian Jiang, Qun Ma, Yuning Hong, Wei Hua Huang, Fan Xia, Xiaoding Lou
Summary: We present a platform for detecting unfolded proteins using electrical and optical signals in a nanochannel array with outer-surface probes. The platform can differentiate folded and unfolded protein states based on the exposure of a single cysteine thiol group, allowing the distinction between normal cells and Huntington's disease mutant cells. This study provides an effective approach for analyzing proteins with distinct conformations and holds promise for facilitating the diagnoses of protein conformation-related diseases.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Meng Meng, Ying Jiang, Wang Li, Qian Wen, Ying Xiong, Qi Chen, Jiali Ren
Summary: In this study, a rapid and sensitive detection method for E. coli based on M13K07 phage was reported. The method was able to detect as low as 4 cfu/mL of E. coli in a short period of time, and specific analysis and practical application were successfully confirmed.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Zhong Cao, Peixian Guo, Shulei Xue, Zhongliang Xiao, Shuqi Tan, Xuan Kang, Zemeng Feng, Yulong Yin, Tianji Ma
Summary: To develop a highly selective method for the detection of L-Arg, researchers screened a polypeptide sequence (CFGHIHEGY) specifically binding to L-Arg from 29185 protein sequences in the Uniprot database. This polypeptide was immobilized on the surface of a bullet shaped nanochannel as a recognition element, enabling selective detection of L-Arg by controlling the effective pore size of the nanochannel. Experimental results showed high sensitivity and selectivity, with a linear range of 1-100 nmol/L and a detection limit as low as 1 nmol/L. This research provides a new method for amino acid sensing and offers a novel approach to designing peptide functionalized bio-inspired ionic channels.
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE
(2023)