A modular two yeast species secretion system for the production and preparative application of unspecific peroxygenases

Two New Unspecific Peroxygenases from Heterologous Expression of Fungal Genes in Escherichia coli

(2020) Dolores Linde et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Identification of novel unspecific peroxygenase chimeras and unusual YfeX axial heme ligand by a versatile high‐throughput GC‐MS approach

(2020) Anja Knorrscheidt et al.   ChemCatChem

Signal Peptides Generated by Attention-Based Neural Networks

(2020) Zachary Wu et al.   ACS Synthetic Biology

Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

(2020) Markus Hobisch et al.   BIOTECHNOLOGY ADVANCES

Current state and future perspectives of engineered and artificial peroxygenases for the oxyfunctionalization of organic molecules

(2020) Marie-Cathérine Sigmund et al.   Nature Catalysis

Benchmarking of laboratory evolved unspecific peroxygenases for the synthesis of human drug metabolites

(2019) Patricia Gomez de Santos et al.   TETRAHEDRON

Construction of a series of episomal plasmids and their application in the development of an efficient CRISPR/Cas9 system in Pichia pastoris

(2019) Yang Gu et al.   WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Peroxygenase‐catalysed epoxidation of styrene derivatives in neat reaction media

(2019) Marine C. R. Rauch et al.   ChemCatChem

Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design

(2019) Pascal Püllmann et al.   Scientific Reports

Functional expression of aryl-alcohol oxidase in Saccharomyces cerevisiae and Pichia pastoris by directed evolution

(2018) Javier Viña-Gonzalez et al.   BIOTECHNOLOGY AND BIOENGINEERING

Side chain removal from corticosteroids by unspecific peroxygenase

(2018) René Ullrich et al.   JOURNAL OF INORGANIC BIOCHEMISTRY

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

(2018) Andreas I. Andreou et al.   PLoS One

Selective Oxidation of Thioanisole with Hydrogen Peroxide using Copper Complexes Encapsulated in Zeolite: Formation of a Thermally Stable and Reactive Copper Hydroperoxo Species

(2018) Syuhei Yamaguchi et al.   ACS Catalysis

Selective Synthesis of the Human Drug Metabolite 5′-Hydroxypropranolol by an Evolved Self-Sufficient Peroxygenase

(2018) Patricia Gomez de Santos et al.   ACS Catalysis

MIDAS: A Modular DNA Assembly System for Synthetic Biology

(2018) Craig J. van Dolleweerd et al.   ACS Synthetic Biology

Shuffling the Neutral Drift of Unspecific Peroxygenase in Saccharomyces cerevisiae

(2018) Javier Martin-Diaz et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization

(2018) Thomas Vogl et al.   Nature Communications

Structural Insights into the Substrate Promiscuity of a Laboratory-Evolved Peroxygenase

(2018) Mercedes Ramirez-Escudero et al.   ACS Chemical Biology

Loop assembly: a simple and open system for recursive fabrication of DNA circuits

(2018) Bernardo Pollak et al.   NEW PHYTOLOGIST

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly

(2018) Vladimir Potapov et al.   ACS Synthetic Biology

A Modular Toolkit for Generating Pichia pastoris Secretion Libraries

(2017) Ulrike Obst et al.   ACS Synthetic Biology

Evolved α-factor prepro-leaders for directed laccase evolution in Saccharomyces cerevisiae

(2017) Ivan Mateljak et al.   Microbial Biotechnology

Synthesis of 1-Naphthol by a Natural Peroxygenase Engineered by Directed Evolution

(2016) Patricia Molina-Espeja et al.   CHEMBIOCHEM

Characterization of a panARS-based episomal vector in the methylotrophic yeast Pichia pastoris for recombinant protein production and synthetic biology applications

(2016) Andrea Camattari et al.   Microbial Cell Factories

Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast

(2016) Ulschan Scheler et al.   Nature Communications

Peroxygenase-Catalyzed Oxyfunctionalization Reactions Promoted by the Complete Oxidation of Methanol

(2015) Yan Ni et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Focused Directed Evolution of Aryl-Alcohol Oxidase in Saccharomyces cerevisiae by Using Chimeric Signal Peptides

(2015) Javier Viña-Gonzalez et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

In-Depth High-Throughput Screening of Protein Engineering Libraries by Split-GFP Direct Crude Cell Extract Data Normalization

(2015) Javier Santos-Aberturas et al.   CHEMISTRY & BIOLOGY

Tandem-yeast expression system for engineering and producing unspecific peroxygenase

(2015) Patricia Molina-Espeja et al.   ENZYME AND MICROBIAL TECHNOLOGY

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

(2015) Thomas Vogl et al.   ACS Synthetic Biology

Directed Evolution of Unspecific Peroxygenase from Agrocybe aegerita

(2014) Patricia Molina-Espeja et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

(2014) Mudassar Ahmad et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Targeted Proteomics Analysis of Protein Degradation in Plant Signaling on an LTQ-Orbitrap Mass Spectrometer

(2014) Petra Majovsky et al.   JOURNAL OF PROTEOME RESEARCH

Mutagenic Organized Recombination Process by Homologous In Vivo Grouping (MORPHING) for Directed Enzyme Evolution

(2014) David Gonzalez-Perez et al.   PLoS One

Hygromycin-resistance vectors for gene expression inPichia pastoris

(2014) Junjie Yang et al.   YEAST

Oxyfunctionalization of aliphatic compounds by a recombinant peroxygenase fromCoprinopsis cinerea

(2013) Esteban D. Babot et al.   BIOTECHNOLOGY AND BIOENGINEERING

An autonomously replicating sequence for use in a wide range of budding yeasts

(2013) Ivan Liachko et al.   FEMS YEAST RESEARCH

Structural Basis of Substrate Conversion in a New Aromatic Peroxygenase

(2013) Klaus Piontek et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants

(2013) Thomas G.M. Schmidt et al.   PROTEIN EXPRESSION AND PURIFICATION

A spectrophotometric assay for the detection of fungal peroxygenases

(2011) Marzena Poraj-Kobielska et al.   ANALYTICAL BIOCHEMISTRY

Specific Photobiocatalytic Oxyfunctionalization Reactions

(2011) Ekaterina Churakova et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Stereoselective benzylic hydroxylation of alkylbenzenes and epoxidation of styrene derivatives catalyzed by the peroxygenase of Agrocybe aegerita

(2011) Martin Kluge et al.   GREEN CHEMISTRY

Comparative genomic analysis of the thermophilic biomass-degrading fungi Myceliophthora thermophila and Thielavia terrestris

(2011) Randy M Berka et al.   NATURE BIOTECHNOLOGY

A Modular Cloning System for Standardized Assembly of Multigene Constructs

(2011) Ernst Weber et al.   PLoS One

GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules

(2011) Alejandro Sarrion-Perdigones et al.   PLoS One

High-yield production of aromatic peroxygenase by the agaric fungus Marasmius rotula

(2011) Glenn Gröbe et al.   AMB Express

Directed evolution of a secretory leader for the improved expression of heterologous proteins and full-length antibodies inSaccharomyces cerevisiae

(2009) J. Andy Rakestraw et al.   BIOTECHNOLOGY AND BIOENGINEERING

Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase

(2009) F Peter Guengerich et al.   Nature Protocols

Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes

(2009) Carola Engler et al.   PLoS One

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One