Article
Multidisciplinary Sciences
Xihong Zhang, Chungen Qian, Li Yang, Huixia Gao, Ping Jiang, Muwei Dai, Yuling Wang, Haiyan Kang, Yi Xu, Qian Hu, Fumin Feng, Bangning Cheng, Erhei Dai
Summary: This study aimed to investigate the value of serum severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid antigen (N-Ag) for COVID-19 diagnosis and analyze N-Ag characteristics in COVID-19 individuals. The results showed that the sensitivity of the N-Ag assay was 64.75% and the specificity was 100%. The study also found a relationship between serum N-Ag and clinical characteristics.
Article
Microbiology
Dalia Raich-Regue, Jordana Munoz-Basagoiti, Daniel Perez-Zsolt, Marc Noguera-Julian, Edwards Pradenas, Eva Riveira-Munoz, Neus Gimenez, Assumpta Carabaza, Francesc Gimenez, Veronica Saludes, Elisa Martro, Neus Robert, Ignacio Blanco, Roger Paredes, Lidia Ruiz, Ester Ballana, Bonaventura Clotet, Julia Blanco, Nuria Izquierdo-Useros
Summary: The SARS-CoV-2 antigen-detecting rapid diagnostic test (Ag-RDTs) plays an important role in identifying new infections, but its sensitivity to different variants varies. Certain Ag-RDTs have shown a more than 10-fold reduction in detection capability for the Delta and Alpha variants, while the Beta and Omicron variants have not been affected. Therefore, continuous monitoring of Ag-RDTs performance is crucial for successful contact tracing.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, General & Internal
Caroline Coulon, Anne-Sophie Bargnoux, Oceane Jourdan, Vincent Foulongne, Anne-Marie Mondain, Anne-Marie Dupuy, Mustapha Sebbane, Jean-Paul Cristol
Summary: In response to the SARS-CoV-2 pandemic, healthcare establishments have implemented effective screening strategies to prevent hospital-acquired infections. Automated antigenic tests have emerged as a reliable alternative to RT-PCR in emergency departments, enhancing patient care. This study shares the deployment experience of LumiraDx antigen tests and compares their results with RT-PCR in a population of emergency department patients.
Article
Microbiology
Jessica Navero-Castillejos, Climent Casals-Pascual, Sofia Narvaez, Genoveva Cuesta, Juan Carlos Hurtado, Mariana Fernandez, Mireia Navarro, Aida Peiro-Mestres, Ma Victoria Lasheras, Patricia Rodriguez, Andrea Pulgarin, Ma Angeles Marcos, Jordi Vila, Miguel Julian Martinez
Summary: In this study, the diagnostic performance of six different commercial rapid antigen tests was evaluated. The sensitivity of the tests ranged from 65% to 79%, while the specificity was 100% for all of them. The Siemens test showed the highest sensitivity for patients with high viral loads, while the Vircell test performed better than the rest for samples with high C-T values.
MICROBIOLOGY SPECTRUM
(2022)
Article
Health Care Sciences & Services
Katherine A. Bond, Ben Smith, Emma Gardiner, K. C. Liew, Eloise Williams, Nicola Walsham, Mark Putland, Deborah A. Williamson
Summary: This study evaluated the application of SARS-CoV-2 rapid antigen testing in an emergency department. The results showed that despite lower sensitivity, the positive predictive value of positive results was high, enabling faster implementation of COVID-19 care pathways and improvement in patient flow within the emergency department.
LANCET REGIONAL HEALTH-WESTERN PACIFIC
(2022)
Article
Infectious Diseases
Bo Diao, Kun Wen, Ji Zhang, Jian Chen, Chao Han, Yongwen Chen, Shufeng Wang, Guohong Deng, Hongwei Zhou, Yuzhang Wu
Summary: The FIC assay for NP antigen testing shows high specificity and relatively high sensitivity in early-phase SARS-CoV-2 diagnosis, according to a study conducted in Wuhan and Chongqing between February 10 and 15, 2020.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Chemistry, Analytical
Jie Liu, Jinpeng Mao, Mengyu Hou, Zhian Hu, Gongwei Sun, Sichun Zhang
Summary: This article presents a rapid and highly specific method for profiling COVID-19 associated antigen. The method utilizes a nanoparticle scaffold and binding-induced DNA assembly mechanism, with fluorescence translation for detection. The study demonstrates that this method can quantify nucleocapsid protein within 3 minutes, with high recognition ratio and low limit of detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Virology
Doyeong Kim, Jihoo Lee, Jyotiranjan Bal, Seul Ki Seo, Chom-Kyu Chong, Jong Ho Lee, Hyun Park
Summary: Antigen tests for SARS-CoV-2 offer a simpler and faster alternative to molecular tests, and clinical validation is crucial before widespread use. The GenBody (TM) COVAG025 immunoassay showed high sensitivity and specificity in both retrospective and prospective clinical studies, indicating its potential to assist in accurate diagnosis, isolation, and treatment of COVID-19 patients for better pandemic control.
Article
Microbiology
Lisa J. Krueger, Andreas K. Lindner, Mary Gaeddert, Frank Tobian, Julian Klein, Salome Steinke, Federica Lainati, Paul Schnitzler, Olga Nikolai, Frank P. Mockenhaupt, Joachim Seybold, Victor M. Corman, Terry C. Jones, Nira R. Pollock, Britta Knorr, Andreas Welker, Stephan Weber, Nandini Sethurarnan, Jayanthi Swaminathan, Hilda Solomon, Ajay Padmanaban, Ma Thirunarayan, L. Prabakaran, Margaretha de Vos, Stefano Ongarello, Jilian A. Sacks, Camille Escadafal, Claudia M. Denkinger
Summary: Our manufacturer-independent prospective study assessed the clinical performance of participants with presumed SARS-CoV-2 infection at three study sites in two countries. We evaluated the accuracy overall and in predefined subgroups based on C-T values and symptom duration. The SureStatus diagnostic test showed high sensitivity, especially in the first few days after symptom onset and when C-T values were low. The system usability and ease-of-use assessment provided valuable insights for the widespread use of SureStatus in point-of-care settings. The high sensitivity demonstrated by the evaluated Ag-RDT during the early phase of infection supports its role in public health screening.
MICROBIOLOGY SPECTRUM
(2022)
Article
Medicine, General & Internal
Jidapa Szekely, Jenureeyah Mongkolprasert, Nitikorn Jeayodae, Chadarat Senorit, Panuttha Chaimuti, Piyawut Swangphon, Natthaphon Nanakorn, Teerapat Nualnoi, Paweena Wongwitwichot, Theerakamol Pengsakul
Summary: The study developed and evaluated an antigen rapid diagnostic test (the Kestrel(TM) COVID-19 Ag Rapid Test) for professional use. The test showed high sensitivity, low limit of detection, and high accuracy in detecting and managing COVID-19, making it a potential tool for screening, surveillance, and infection control in global SARS-CoV-2 variant outbreaks.
Article
Multidisciplinary Sciences
Erica Frew, Douglas Roberts, Shelly Barry, Matthew Holden, Amanda Restell Mand, Emily Mitsock, Enqing Tan, Wei Yu, Johan Skog
Summary: To address the challenges posed by the novel coronavirus, a cost-effective and rapid SARS-CoV-2 antigen test has been developed, capable of identifying the presence of the virus in patient anterior nares swab samples with high sensitivity and no requirement for equipment or laboratory infrastructure.
SCIENTIFIC REPORTS
(2021)
Article
Nanoscience & Nanotechnology
Fatemeh Haghayegh, Razieh Salahandish, Mohsen Hassani, Amir Sanati-Nezhad
Summary: This study presents a stable buffer-based zinc oxide/reduced graphene oxide nanocomposite coated on carbon screen-printed electrodes for the electrochemical immuno-biosensing of SARS-CoV-2 protein antigens. The biosensor shows a low limit of detection, stable sensor nanocoating, high sensitivity, and the ability to rapidly discriminate positive and negative clinical samples.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Virology
Anna Heydecke, Karolina Gullsby
Summary: During the COVID-19 pandemic, the Roche SARS-CoV-2 Rapid Antigen Test (Ag-RDT) was evaluated for its performance in an emergency care setting. The Ag-RDT's analytical performance was compared to rRT-PCR, and its clinical evaluation included 1911 patients. The Ag-RDT showed a sensitivity of 71.3% and a specificity of 99.7%, with an increased sensitivity of 86.5% when excluding samples with Ct values above 30. The test also shortened the detection time by 11 hours on average.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Medicine, General & Internal
Bilgin Osmanodja, Klemens Budde, Daniel Zickler, Marcel G. Naik, Joerg Hofmann, Maximilian Gertler, Claudia Huelso, Heike Roessig, Philipp Horn, Joachim Seybold, Stephanie Lunow, Melanie Bothmann, Astrid Barrera-Pesek, Manuel Mayrdorfer
Summary: A novel Ag-RDT for anterior nasal self-collection has been validated with high sensitivity and specificity, providing a valuable alternative for sample collection.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Health Care Sciences & Services
Heekyung Lee, Hyunggoo Kang, Yongil Cho, Jaehoon Oh, Tae-Ho Lim, Byuk-Sung Ko, Juncheol Lee
Summary: The diagnostic value of the SARS-CoV-2 rapid antigen test (RAT) as a screening tool in the emergency department (ED) was investigated in this study. The RAT showed high sensitivity as a screening tool in the ED, especially in patients with a high viral load.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Article
Infectious Diseases
Matthaios Papadimitriou-Olivgeris, Damien Jacot, Laurence Senn, Benoit Guery
Summary: This retrospective study compared patients with Staphylococcus aureus bacteraemia (SABA) with or without concomitant bacteriuria (SABU) at Lausanne University Hospital from 2015 to 2021. Out of 448 included bacteraemic patients, 62 (13.8%) had concurrent S. aureus isolated from urine. The study found significant differences in community-onset bacteraemia, malignancy, > 1 pair of positive blood cultures, and persistent bacteraemia for at least 48 h in patients with concurrent SABU. However, there was no difference in mortality. SABU was associated with higher rates of SABA recurrence after antibiotic cessation.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Matthaios Papadimitriou-Olivgeris, Giorgia Caruana, Laurence Senn, Benoit Guery
Summary: This study aimed to identify predictors of mortality among patients with S. aureus bacteremia and evaluate the role of early source control. The results showed that high mortality was associated with factors such as Charlson comorbidity index > 5, nosocomial bacteremia, time to blood culture positivity <= 13 h, persistent bacteremia for >= 48 h, sepsis, bacteremia of unknown origin, and lower respiratory tract infection. Infectious diseases consultation within 48 h was associated with better survival. Early source control was also found to be associated with better survival.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Editorial Material
Immunology
Eleftheria Kampouri, Oriol Manuel
TRANSPLANT INFECTIOUS DISEASE
(2023)
Article
Immunology
Marc-Olivier Boldi, Justin Denis-Lessard, Rina Neziri, Rene Brouillet, Christophe von-Garnier, Valerie Chavez, Jesica Mazza-Stalder, Katia Jaton, Gilbert Greub, Onya Opota
Summary: Polymerase chain reactions (PCRs) have a significant impact on the diagnosis of tuberculosis (TB), with higher sensitivity and specificity than microscopy. Bronchial aspirate has a higher diagnostic yield than bronchoalveolar lavage (BAL) and sputum in respiratory specimens. Therefore, PCR should be performed on bronchial aspirates when available.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Jose Knuesli, Loic Lhopitallier, Andreas Kronenberg, Jean-Yves Meuwly, Onya Opota, Marc-Antoine Perrenoud, Marie-Anne Page, Kevin C. Kain, Aline Mamin, Valerie D'Acremont, Nicolas Senn, Yolanda Mueller, Isabella Locatelli, Noemie Boillat-Blanco
Summary: In a clinical trial in Switzerland, one-third of patients with low procalcitonin level received antibiotics, which was associated with the socio-demographic characteristics of the physicians. This highlights the importance of continuous medical education and communication training for successful implementation of procalcitonin point-of-care tests for antibiotic stewardship.
Article
Surgery
Philip Deslarzes, Jonas Jurt, Martin Hubner, Dieter Hahnloser, Daniel Clerc, Laurence Senn, Nicolas Demartines, Fabian Grass
Article
Immunology
Matteo Mombelli, Dionysios Neofytos, Uyen Huynh-Do, Javier Sanchez-Cespedes, Susanne Stampf, Dela Golshayan, Suzan Dahdal, Guido Stirnimann, Aurelia Schnyder, Christian Garzoni, Reto M. Venzin, Lorenzo Magenta, Melanie Schonenberger, Laura Walti, Cedric Hirzel, Aline Munting, Michael Dickenmann, Michael Koller, John-David Aubert, Jurg Steiger, Manuel Pascual, Thomas F. Mueller, Mace Schuurmans, Christoph Berger, Isabelle Binet, Jean Villard, Nicolas J. Mueller, Adrian Egli, Elisa Cordero, Christian van Delden, Oriol Manuel
Summary: The use of MF59-adjuvanted or high-dose influenza vaccine in solid-organ transplant recipients was safe and resulted in a higher vaccine response rate.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Eleftheria Kampouri, Danniel Zamora, Erika S. Kiem, Winnie Liu, Sarah Ibrahimi, Rachel L. Blazevic, Erika A. Lovas, Louise E. Kimball, Meei-Li Huang, Keith R. Jerome, Masumi Ueda Oshima, Marco Mielcarek, Danielle M. Zerr, Michael J. Boeckh, Elizabeth M. Krantz, Joshua A. Hill
Summary: The use of letermovir for cytomegalovirus (CMV) prophylaxis has led to a decrease in broad-spectrum anti-CMV therapy. However, the impact of letermovir on human herpesvirus-6 (HHV-6) epidemiology remains unknown. This retrospective cohort study aimed to assess changes in HHV-6 detection and disease after the routine use of prophylactic letermovir in CMV-seropositive allogeneic hematopoietic cell transplant recipients.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Public, Environmental & Occupational Health
M. Van Singer, L. Senn, D. S. Blanc, I. Koenig, C. Simon, B. Grandbastien
JOURNAL OF HOSPITAL INFECTION
(2023)
Review
Immunology
Eleftheria Kampouri, Joshua A. Hill, Veronica Dioverti
Summary: More than 3 years have passed since the COVID-19 pandemic began, but immunocompromised individuals, particularly those receiving hematopoietic cell transplantation and chimeric antigen receptor-T-cell therapies, continue to be at high risk for severe COVID-19 and mortality. Strategies such as antiviral therapies, convalescent plasma, and novel therapies targeting SARS-CoV-2-specific T-cells and natural killer cells show promise in preventing and treating COVID-19 in this vulnerable population.
TRANSPLANT INFECTIOUS DISEASE
(2023)
Review
Immunology
Eleftheria Kampouri, Jessica S. Little, Kai Rejeski, Oriol Manuel, Sarah P. Hammond, Joshua A. Hill
Summary: CAR-T-cell therapies have greatly improved the treatment of blood cancers, but they come with unique toxicities, especially infections. Infection incidence is highest in the first month after treatment and gradually decreases afterwards. Different CAR-T-cell therapies also result in different types of infections.
TRANSPLANT INFECTIOUS DISEASE
(2023)
Article
Public, Environmental & Occupational Health
Elisavet Stavropoulou, Andrew Atkinson, Marie-Christine Eisenring, Christoph A. Fux, Jonas Marschall, Laurence Senn, Nicolas Troillet
Summary: This study compared the effectiveness of A/CA and C + M in preventing SSI in colorectal surgery and found that A/CA was less effective than C + M. The study suggests that A/CA should not be the first choice for antibiotic prophylaxis in colorectal surgery.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2023)
Article
Ophthalmology
Nan-Ji Lu, Hendrik Koliwer-Brandl, Mark Hillen, Adrian Egli, Farhad Hafezi
Summary: This study aimed to compare the efficacy of riboflavin/UV-A light and rose bengal/green light in treating Staphylococcus aureus and Pseudomonas aeruginosa infections. The results demonstrated that rose bengal/green light had significantly higher bacterial killing ratios compared to riboflavin/UV-A light.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2023)
Article
Microbiology
Vincent Vanat, Sebastien Aeby, Gilbert Greub
Summary: Ticks are important vectors for medical pathogens. This research found that zoological gardens in Switzerland have fewer ticks than surrounding areas, but the ticks in zoos are more likely to carry Chlamydia-like organisms compared to ticks in control areas.
Article
Medicine, General & Internal
Alix T. Coste, Adrian Egli, Jacques Schrenzel, Beatrice Nickel, Andrea Zbinden, Reto Lienhard, Alexis Dumoulin, Martin Risch, Gilbert Greub
Summary: The IVDR regulation poses a major challenge for diagnostic microbiology laboratories due to its complexity and risks. The emergence of new pathogens further complicates the regulation, negatively impacting the development of new diagnostic tests. Additionally, the regulation affects the availability and costs of diagnostic tests, posing a risk to public health.