Article
Cell Biology
Tian Xia, Ming Liu, Quan Zhao, Jian Ouyang, Bing Chen, Peipei Xu
Summary: PRMT5 is upregulated in multiple myeloma, and its inhibition enhances cell pyroptosis, while the expression of CASP1 negatively correlated with PRMT5 can be restored by suppressing PRMT5, promoting cell pyroptosis in MM.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Chengrui Shi, Xikang Yang, Ye Liu, Hongpeng Li, Huiying Chu, Guohui Li, Hang Yin
Summary: Palmitoylation of cGAS at C474 restricts its enzymatic activity in the presence of double-stranded DNA, providing a novel regulatory mechanism for innate immunity.
Article
Multidisciplinary Sciences
Yu-Lin Yang, Li -Bo Cao, Wen-Rui He, Li Zhong, Yi Guo, Qing Yang, Hong -Bing Shu, Ming-Ming Hu
Summary: The current understanding of nucleic acid-mediated innate immunity is that the binding of intracellular sensors to nucleic acids is enough to activate them. However, this study reveals that the endocytosis of viruses or foreign DNA provides a priming signal for the activation of the DNA sensor cGAS in the innate immune response. This finding demonstrates that the activation of cGAS following endocytosis requires tyrosine phosphorylation mediated by the V-ATPase-SYK pathway.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Anyi Liu, Chengxin Yu, Cheng Qiu, Qi Wu, Changsheng Huang, Xun Li, Xiaowei She, Kairui Wan, Lang Liu, Mao Li, Zhihong Wang, Yaqi Chen, Fuqing Hu, Da Song, Kangdi Li, Chongchong Zhao, Haiteng Deng, Xuling Sun, Feng Xu, Senyan Lai, Xuelai Luo, Junbo Hu, Guihua Wang
Summary: Perturbations in TGF-beta signaling can be caused by mutations and posttranslational modifications of SMAD4, including R361 methylation mediated by PRMT5. This study demonstrates that the interaction between PRMT5 and SMAD4, as well as SMAD4 R361 methylation, play critical roles in TGF-beta signaling activation and metastasis. Blocking PRMT5-SMAD4 signaling could be a potential therapeutic strategy for SMAD4 wild-type CRC.
Article
Fisheries
Juan Du, Han Xiao, Yanqiu Hu, Zhi Li
Summary: Grass carp is an economically important fish, but the viral hemorrhagic diseases caused by grass carp reovirus (GCRV) can significantly impact its economic yield. This study focused on the role of Cimarch2 in the antiviral innate immune response in grass carp. The results showed that Cimarch2 has high homology in grass carp and other species, and it can regulate interferon activation and promote viral replication through the downregulation of tbk1 via the proteasome pathway. These findings suggest the potential of Cimarch2 in antiviral breeding of grass carp.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Microbiology
Xiaohui Ju, Yanying Yu, Wenlin Ren, Lin Dong, Xianbin Meng, Haiteng Deng, Yuchen Nan, Qiang Ding
Summary: This study systematically analyzed the host factors in HEV replication complex using ORF1 trans-complementation system and HEV replicon. The PRMT5/WDR77 complex was found to have an inhibitory role in HEV infection among different HEV strains, but not in HCV and SARS-CoV-2 infection. The complex methylates the 458th arginine in HEV ORF1, which is responsible for HEV replication. The findings provide insights into HEV replication and viral-host interaction, and inform antiviral strategies against HEV infection.
Article
Immunology
Hongyun Wang, Jiangpeng Feng, Cong Zeng, Jiejie Liu, Zhiying Fu, Dehe Wang, Yafen Wang, Lu Zhang, Jiali Li, Ao Jiang, Miao He, Yuanyuan Cao, Kun Yan, Hao Tang, Deyin Guo, Ke Xu, Xiang Zhou, Li Zhou, Ke Lan, Yu Zhou, Yu Chen
Summary: NSUN2, a typical m(5)C methyltransferase, negatively regulates type I interferon responses during various viral infections by mediating m(5)C methylation of IRF3 mRNA and accelerating its degradation. Knockout or knockdown of NSUN2 enhances type I interferon and downstream ISGs during viral infection in vitro. Moreover, various viral infections decrease endogenous levels of NSUN2, further increasing type I interferon and downstream ISGs.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Hematology
Shelby L. Sloan, Fiona Brown, Mackenzie Long, Christoph Weigel, Shirsha Koirala, Ji-Hyun Chung, Betsy Pray, Lynda Villagomez, Claire Hinterschied, Anuvrat Sircar, Jobeth Helmig-Mason, Alexander Prouty, Eric Brooks, Youssef Youssef, Walter Hanel, Samir Parekh, Wing Keung Chan, Zhengming Chen, Rosa Lapalombella, Lalit Sehgal, Kris Vaddi, Peggy Scherle, Selina Chen-Kiang, Maurizio Di Liberto, Olivier Elemento, Cem Meydan, Jonathan Foox, Daniel Butler, Christopher E. Mason, Robert A. Baiocchi, Lapo Alinari
Summary: Inhibition of PRMT5 in mantle cell lymphoma demonstrated antitumor activity and restored regulatory activity of the cell cycle, apoptotic cell death, and negative regulators of the B-cell receptor-PI3K/AKT signaling pathway. The selective targeting of PRMT5 shows promise as a potential therapy for patients with relapsed/refractory MCL.
Article
Hematology
Malini Rethnam, Darren Qiancheng Tan, Shi Hao Tan, Jia Li, Rui Yokomori, Ying Li, Henry Yang, Takaomi Sanda, Toshio Suda
Summary: The rare and aggressive hematologic malignancy BPDCN is highly sensitive to PRMT5 inhibition, which not only inhibits cell growth but also mitigates tumor progression. The inhibition of PRMT5 affects the function of key RNA methylation gene METTL3, leading to increased interferon signaling and attenuated sensitivity to PRMT5 inhibition.
Article
Chemistry, Multidisciplinary
Xiao-liang Dong, Bao-hui Yuan, Sheng-zhou Yu, He Liu, Xiao-hua Pan, Jia Sun, Li-long Pan
Summary: Long-term treatment with adriamycin (ADR) can lead to cardiac fibrosis, and PRMT5 plays a role in this process by activating cardiac fibroblasts and promoting ADR-induced cardiac fibrosis. PRMT5 may be a potential therapeutic target for ADR-caused cardiotoxicity.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Oncology
Shuangjie Liu, Zhuonan Liu, Chiyuan Piao, Zhe Zhang, Chuize Kong, Lei Yin, Xi Liu
Summary: The study reveals PRMT5 as a therapeutic target for bladder cancer and identifies FKA, extracted from the kava plant, as an inhibitor of PRMT5 for the treatment of bladder cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Kun Ho Kim, Zhihao Jia, Madigan Snyder, Jingjuan Chen, Jiamin Qiu, Stephanie N. Oprescu, Xiyue Chen, Sabriya A. Syed, Feng Yue, Bruno T. Roseguini, Anthony N. Imbalzano, Changdeng Hu, Shihuan Kuang
Summary: Protein Arginine Methyl Transferase 5 (PRMT5) plays a crucial role in skeletal muscle by linking lipid metabolism to contractile function. Deletion of PRMT5 in skeletal muscle leads to reduced muscle mass, oxidative capacity, force production, and exercise performance. This deficiency is associated with defects in lipid biosynthesis, accelerated degradation, and reduced levels of key regulators such as SREBP1a and ATGL. Restoring the balance of these regulators can normalize muscle mass and function.
Article
Biology
Baskar Chakrapani, Mohd Imran K. Khan, Rajashekar Varma Kadumuri, Somlee Gupta, Mamta Verma, Sharad Awasthi, Gayathri Govindaraju, Arun Mahesh, Arumugam Rajavelu, Sreenivas Chavali, Arunkumar Dhayalan
Summary: PRMT5 is an enzyme that symmetrically dimethylates arginine residues in various proteins, and its overexpression is associated with several types of cancers. The study identified FAM47E as an interaction partner of PRMT5, which regulates PRMT5's stability and activity, affecting its functions and levels.
LIFE SCIENCE ALLIANCE
(2021)
Article
Cell Biology
He Liu, Kunpeng Jia, Zhengnan Ren, Jia Sun, Li-Long Pan
Summary: A high plasma level of trimethylamine N-oxide (TMAO) is closely associated with the development of cardiovascular disease. This study demonstrates that protein arginine methyltransferase 5 (PRMT5) expression is positively correlated with TMAO-induced vascular inflammation, leading to increased vascular cell adhesion molecule-1 (VCAM-1) expression. Knockdown of PRMT5 inhibits VCAM-1 expression and macrophage adhesion to TMAO-stimulated vascular smooth muscle cells (VSMC). The study also reveals that TMAO induces nicotinamide adenine dinucleotide phosphate oxidase 4 (Nox4) expression and reactive oxygen species production, which contributes to PRMT5 expression and subsequent VCAM-1 expression.
CELL DEATH & DISEASE
(2022)
Article
Microbiology
Salik Miskat Borbora, Raju S. Rajmani, Kithiganahalli Narayanaswamy Balaji
Summary: Mycobacterium tuberculosis infection can lead to the formation of lipid-rich foamy macrophages (FMs) that provide a favorable environment for pathogen persistence. During infection, the expression of the E3 ubiquitin ligase ITCH is significantly reduced, allowing key lipid accumulation molecules (ADRP and CD36) to be spared from proteasomal degradation. Furthermore, this repression of ITCH depends on the coordinated action of the bifunctional transcription factor YY1 and the arginine methyl transferase PRMT5. The NOTCH signaling pathway is identified as a master regulator of YY1 expression. In vitro and in vivo analyses demonstrate the importance of PRMT5 in regulating FM formation and mycobacterial burden.
Article
Rheumatology
Zhaowen Yang, Jin Cao, Chengcheng Yu, Qingrui Yang, Yuanchao Zhang, Lihui Han
Article
Multidisciplinary Sciences
Wanwan Huai, Hui Song, Zhongxia Yu, Wenwen Wang, Lihui Han, Takeharu Sakamoto, Motoharu Seiki, Lining Zhang, Qunye Zhang, Wei Zhao
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Medicine, General & Internal
Ying Zhang, Tao Li, Yumin Qiu, Tao Zhang, Pengbo Guo, Xiaomin Ma, Qing Wei, Lihui Han
Article
Biochemistry & Molecular Biology
P. Guo, X. Ma, W. Zhao, W. Huai, T. Li, Y. Qiu, Y. Zhang, L. Han
Article
Oncology
Pengbo Guo, Yumin Qiu, Xiaomin Ma, Tao Li, Xiaoxiao Ma, Lihui Zhu, Yueke Lin, Lihui Han
EXPERIMENTAL CELL RESEARCH
(2018)
Article
Cell Biology
Xiaoxiao Ma, Xiaomin Ma, Yumin Qiu, Lihui Zhu, Yueke Lin, Yajing You, Dapeng Ma, Zhenzhi Qin, Caiyu Sun, Yunxue Zhao, Yanlin Sun, Lihui Han
CELL DEATH & DISEASE
(2018)
Article
Biochemistry & Molecular Biology
Yumin Qiu, Peishu Liu, Xiaomin Ma, Xiaoxiao Ma, Lihui Zhu, Yueke Lin, Yajing You, Wenhao Yu, Dapeng Ma, Caiyu Sun, Zhenzhi Qin, Yunxue Zhao, Juanjuan Shi, Lihui Han
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Lihui Zhu, Chengyong Qin, Tao Li, Xiaomin Ma, Yumin Qiu, Yueke Lin, Dapeng Ma, Zhenzhi Qin, Caiyu Sun, Xuecheng Shen, Yunxue Zhao, Lihui Han
CELL DEATH AND DIFFERENTIATION
(2020)
Article
Genetics & Heredity
Xiaomin Ma, Yumin Qiu, Lihui Zhu, Yunxue Zhao, Yueke Lin, Dapeng Ma, Zhenzhi Qin, Caiyu Sun, Xuecheng Shen, Tao Li, Lihui Han
JOURNAL OF MOLECULAR MEDICINE-JMM
(2020)
Article
Cell Biology
Xiaomin Ma, Yumin Qiu, Yanlin Sun, Lihui Zhu, Yunxue Zhao, Tao Li, Yueke Lin, Dapeng Ma, Zhenzhi Qin, Caiyu Sun, Lihui Han
CELL DEATH & DISEASE
(2020)
Article
Oncology
Xiaomin Ma, Xiaoxiao Ma, Lihui Zhu, Yunxue Zhao, Mengmeng Chen, Tao Li, Yueke Lin, Dapeng Ma, Caiyu Sun, Lihui Han
Summary: In this study, we found that MG53 acts as an inhibitor of RAC1 and a tumor suppressor in hepatocellular carcinoma (HCC) by catalyzing RAC1 ubiquitination and inhibiting RAC1 activity. Furthermore, MG53 suppressed the malignant behaviors of HCC cells and enhanced chemosensitivity to sorafenib treatment.