Article
Biophysics
Jayeon Song, Hyo Yong Kim, Soohyun Kim, Yujin Jung, Hyun Gyu Park
Summary: A novel technology, SP-HAMP, is described for target nucleic acid detection under isothermal conditions. By designing a unique hairpin probe, it is possible to detect target DNA with outstanding discrimination capability using only a single primer, without the need for complex multiple primers.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh
Summary: This review outlines several techniques for reducing false-positive LAMP results before and after amplification. Organic additives or pullulan can be used to decrease nonspecific amplification before amplification. Uracil-DNA-glycosylase and the hot-start effect with gold nanoparticles can eliminate carry-over contamination and reduce nonspecific amplification after amplification. The use of guide RNA and colorimetric change in DNAzyme can accurately confirm amplification results.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Nan Wang, Jie Gao, Enjing Tian, Wenjie Yu, Hui Li, Juan Zhang, Ruibin Xie, Ailiang Chen
Summary: In this study, LAMP assays were developed to detect R. senecis, a common poisonous mushroom in China, with high sensitivity and applicability, providing a valuable method for clinical sample detection and forensic identification.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yajuan Dong, Xiuming Wu, Shenwei Li, Renfei Lu, Yingxue Li, Zhenzhou Wan, Jianru Qin, Guoying Yu, Xia Jin, Chiyu Zhang
Summary: This study compared the performance of 19 sets of primers in COVID-19 detection and identified 6 sets of primers with faster amplification. Among them, the Set-4 primer set had the highest positive detection rate of SARS-CoV-2.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Hao Yuan, Jingxuan Tian, Youchuang Chao, Yuh-Shiuan Chien, Ren-Hao Luo, Jun-Yu Guo, Shanshan Li, Yi-Ju Chou, Ho Cheung Shum, Chien-Fu Chen
Summary: ddLAMP is a crucial pathogen detection technique, and the simplified version developed significantly reduces operational difficulty and equipment requirements, making it accessible even to untrained personnel.
Article
Biochemistry & Molecular Biology
Hye-Soon Song, Hyeon-Su Kim, Ji-Ye Kim, Yong-Kuk Kwon, Hye-Ryoung Kim
Summary: Newcastle disease (ND) is a highly pathogenic viral infection of poultry with significant economic impacts worldwide. Novel sets of visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays have been developed for the rapid detection and differentiation of NDV, showing potential for field or resource-limited environments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Daniel Urrutia-Cabrera, Roxanne Hsiang-Chi Liou, Jiang-Hui Wang, Jianxiong Chan, Sandy Shen-Chi Hung, Alex W. Hewitt, Keith R. Martin, Thomas L. Edwards, Patrick Kwan, Raymond Ching-Bong Wong
Summary: This study compares the performance of three LAMP-based assays targeting different regions of SARS-CoV-2, showing that all three assays can accurately detect 1000 RNA copies in 30 minutes, with the Gene N assay exhibiting no false positives. This highlights the significance of LAMP as a rapid, highly sensitive detection method for SARS-CoV-2 diagnostics.
SCIENTIFIC REPORTS
(2021)
Review
Plant Sciences
Aleksandr Ivanov, Irina Safenkova, Anatoly Zherdev, Boris B. Dzantiev
Summary: Rapid, sensitive, and timely diagnostics are crucial for protecting plants from pathogens, with isothermal amplification methods providing a feasible solution for in-field molecular diagnostics. Combining isothermal amplification techniques with extraction and detection methods compatible with outdoor phytodiagnostics is essential for preventing and controlling the spread of disease caused by viral, bacterial, and fungal phytopathogens. The development of rapid, sensitive, and equipment-free nucleic acid detection methods is seen as the future of phytodiagnostics, showcasing visible benefits.
Article
Chemistry, Analytical
Rui Mao, Tianzuo Wang, Yue Zhao, Xinyao Wu, Shun Zhang, Ting Cai
Summary: The article presents a novel isothermal amplification method called closed dumbbell mediated isothermal amplification (CDA) for nucleic acids. The method offers the advantages of simple primer design, high amplification efficiency, and successful detection of virus genes and bacterial pathogens. Real-time and colorimetric CDA detection methods have also been developed and proven effective.
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemical Research Methods
MinJu Jang, Sanghyo Kim
Summary: Loop-mediated isothermal amplification (LAMP) is a molecular and point-of-care diagnostic technique used for pathogen detection, but non-specific amplification often occurs. In this study, we discovered that tetramethylammonium chloride (TMAC) can effectively suppress non-specific amplification in LAMP by using additives.
Article
Agriculture, Multidisciplinary
David Marek, Daniel Eberl, Khaled Otba, Ruth Walter, Andreas Kortekamp, Wilfried Schwab, Ludwig Niessen
Summary: This study developed a LAMP-based molecular detection method for Esca pathogens and demonstrated its application to field samples in a rapid sample preparation step. The study also showed the usefulness of the genome-based approach for identifying target sequences in species with limited sequence information. Additionally, the developed rapid DNA preparation method may have potential applications in the DNA-based detection of other fungal species.
ANNALS OF APPLIED BIOLOGY
(2023)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Biotechnology & Applied Microbiology
Rui Mao, Xinyao Wu, Qing Miao, Ting Cai
Summary: Nucleic acid-based assays, such as Loop-mediated isothermal amplification (LAMP), have been widely used in clinical diagnostics, food safety, and environmental monitoring. A novel method called asymmetric stem-loop-mediated isothermal amplification (ASLAMP) was developed to overcome the challenges of specific primer design and non-specific amplification associated with LAMP. ASLAMP showed promising results in detecting the H1N1 gene fragment with simple primer design, short target sequence, and high amplification efficiency, offering potential for point-of-care DNA isothermal amplification.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Meeting Abstract
Oncology
Laimonas Kelbauskas, Jia Zeng, Aida Rezaie, Kristen Lee, Benjamin Ueberroth, Weimin Gao, Dmitry Derkach, Colleen Ziegler, Thai Tran, Kimberly J. Bussey, Dean Smith, Roger H. Johnson, Deirdre R. Meldrum
Article
Biochemical Research Methods
Xu Shi, Chun-Hong Chen, Weimin Gao, Shih-hui Chao, Deirdre R. Meldrum
Article
Microbiology
Christopher L. Hemme, Qichao Tu, Zhou Shi, Yujia Qin, Weimin Gao, Ye Deng, Joy D. Van Nostrand, Liyou Wu, Zhili He, Patrick S. G. Chain, Susannah G. Tringe, Matthew W. Fields, Edward M. Rubin, James M. Tiedje, Terry C. Hazen, Adam P. Arkin, Jizhong Zhou
FRONTIERS IN MICROBIOLOGY
(2015)
Meeting Abstract
Oncology
Laimonas Kelbauskas, Shashanka Ashili, Jia Zeng, Aida Mohammadreza, Kristen Lee, Dmitry Derkach, Benjamin Ueberroth, Weimin Gao, Thomas Paulson, Hong Wang, Yanqing Tian, Dean Smith, Brian Reid, Deirdre Meldrum
Article
Chemistry, Multidisciplinary
Liqiang Zhang, Fengyu Su, Xiangxing Kong, Fred Lee, Kevin Day, Weimin Gao, Mary E. Vecera, Jeremy M. Sohr, Sean Buizer, Yanqing Tian, Deirdre R. Meldrum
Article
Multidisciplinary Sciences
Jia Zeng, Laimonas Kelbauskas, Aida Rezaie, Kristen Lee, Benjamin Ueberroth, Weimin Gao, Dmitry Derkach, Thai Tran, Dean Smith, Kimberly J. Bussey, Deirdre R. Meldrum
SCIENTIFIC REPORTS
(2016)
Article
Multidisciplinary Sciences
L. Kelbauskas, S. Ashili, J. Zeng, A. Rezaie, K. Lee, D. Derkach, B. Ueberroth, W. Gao, T. Paulson, H. Wang, Y. Tian, D. Smith, B. Reid, Deirdre R. Meldrum
SCIENTIFIC REPORTS
(2017)
Review
Biotechnology & Applied Microbiology
Xu Shi, Weimin Gao, Jiangxin Wang, Shih-Hui Chao, Weiwen Zhang, Deirdre R. Meldrum
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2015)
Article
Oncology
Lusheng Song, Minkyo Song, M. Constanza Camargo, Jennifer Van Duine, Stacy Williams, Yunro Chung, Kyoung-Mee Kim, Jolanta Lissowska, Armands Sivins, Weimin Gao, Kailash Karthikeyan, Jin Park, Marcis Leja, Jeffrey I. Cohen, Joshua LaBaer, Ji Qiu, Charles S. Rabkin
Summary: The study found differences in the antibody responses to specific antigens between EBV-positive and EBV-negative GC patients, with certain antibodies potentially serving as biomarkers to distinguish tumor EBV status. These specific anti-EBV antibodies could be useful for clinical diagnosis, epidemiological studies, and immune-based precision treatment of EBV-positive GC.
Article
Oncology
Mahasish Shome, Weimin Gao, Anna Engelbrektson, Lusheng Song, Stacy Williams, Vel Murugan, Jin G. Park, Yunro Chung, Joshua LaBaer, Ji Qiu
Summary: This study used protein microarrays to detect antibodies in the serum of lung adenocarcinoma (ADC), smoker controls (SMC), and benign nodule controls (BNC) patients, and found antimicrobial antibodies associated with ADC and BNC. These antibodies help distinguish malignant and benign nodules detected by CT scan and have the potential to reduce false positive rates.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2023)
Article
Biochemical Research Methods
Lusheng Song, Minkyo Song, Charles S. Rabkin, Stacy Williams, Yunro Chung, Jennifer Van Duine, Linda M. Liao, Kailash Karthikeyan, Weimin Gao, Jin G. Park, Yanyang Tang, Jolanta Lissowska, Ji Qiu, Joshua LaBaer, M. Constanza Camargo
Summary: This study evaluated humoral responses to a nearly complete H. pylori immunoproteome among GC cases and controls, finding higher prevalence of certain antibodies in controls and lower seroprevalence in GC cases, suggesting immune protection and potential changes in specific proteins.
JOURNAL OF PROTEOME RESEARCH
(2021)
Meeting Abstract
Oncology
Lusheng Song, Minkyo Song, M. Constanza Camargo, Jennifer Van Duine, Stacy Williams, Yunro Chung, Kyoung-Mee Kim, Jolanta Lissowska, Sergejs Isajevs, Sung Kim, Weimin Gao, Kailash Karthikeyan, Jin Park, Marcis Leja, Jeffrey L. Cohen, Joshua LaBaer, Ji Qiu, Charles S. Rabkin
Meeting Abstract
Gastroenterology & Hepatology
Lusheng Song, Minkyo Song, Charles S. Rabkin, Jennifer Van Duine, Linda Liao, Kailash Karthikeyan, Weimin Gao, Jolanta Lissowska, Ji Qiu, Joshua LaBaer, M. Constanza Camargo