Article
Parasitology
Luis Carvalho, Maria L. Felix, Valentin Bazzano, Anthony da Costa, Maria T. Armua-Fernandez, Sebastian Munoz-Leal, Jose Manuel Venzal
Summary: Hepatozoon parasites related to H. americanum were detected in road-killed foxes in Uruguay. Phylogenetic analyses showed clustering with H. americanum from the United States and similar species from dogs and foxes in Brazil and Argentina. The findings suggest a potential veterinary concern due to the overlap in habitat between dogs and foxes.
PARASITOLOGY RESEARCH
(2021)
Article
Immunology
Thamiris Cardoso Padilha, Larissa Calo Zitelli, Anelise Webster, Bruno Dall'Agnol, Veronica Bueno da Rosa, Ugo Souza, Felipe Bortolotto Peters, Marcia Jardim, Tatiane Campos Trigo, Rogerio Oliveira Rodrigues, Fernanda Simone Marks, Jose Reck
Summary: The expansion of urbanization is increasing human-wildlife contact, leading to potential spread of diseases in wild carnivores. This study found that wild free-ranging Guiana foxes and hoary foxes in southern Brazil were exposed to multiple pathogens, with higher infection rates observed for Toxoplasma gondii, Trypanosoma cruzi, and Leptospira spp.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2021)
Article
Agriculture, Dairy & Animal Science
Bruna Elenara Szynwelski, Rafael Kretschmer, Cristina Araujo Matzenbacher, Flavia Ferrari, Marcelo Meller Alievi, Thales Renato Ochotorena de Freitas
Summary: A female canid with intermediate characteristics between a domestic dog and a pampas fox was found in Vacaria, Brazil. Genetic analysis confirmed it to be a hybrid between the two species, which is the first documented case of such hybridization in South America. This finding poses a threat to the conservation of wild canids, and further studies are needed to understand its genetic, ecological, and behavioral consequences.
Article
Infectious Diseases
Cintia De Lorenzo, Fabiana Marques Boabaid, Luiz Gustavo Schneider de Oliveira, Matheus Viezzer Bianchi, Maria L. Felix, Maria T. Armua-Fernandez, Joao Fabio Soares, Jose M. Venzal, Luciana Sonne
Summary: Rangelia vitalii is a protozoan parasite causing hemorrhagic and hemolytic disease in dogs, mainly found in southern and southeastern regions of Brazil. This study aimed to detect R. vitalii parasitism in the Uruguayan wild fox population, with positive samples found in crab-eating foxes but not in pampas foxes. Molecular analysis suggests circulation of R. vitalii in the crab-eating fox population in Uruguay, with unknown veterinary relevance for these foxes.
TICKS AND TICK-BORNE DISEASES
(2021)
Article
Virology
Diego Fernando Echeverry-Bonilla, Edwin Fernando Buritica-Gaviria, Delio Orjuela-Acosta, Danny Jair Chinchilla-Cardenas, Julian Ruiz-Saenz
Summary: We confirmed the presence of the South America/North America-4 lineage of canine distemper virus (CDV) in naturally infected Crab-eating Fox for the first time. Our findings are crucial for understanding the interspecies transmission of CDV in the domestic/wild interface and for the prevention and control of this important multi-host pathogen.
Article
Agriculture, Dairy & Animal Science
Noelia Zambra, Lorena Lacuesta, Agustin Orihuela, Rodolfo Ungerfeld
Summary: The study found that ewes exposed to the odour of fox and wild boar faeces exhibited more aggressive behaviors and locomotion behaviors. Although physiological responses were not affected by the odour, ewes responded to the odour of predators with a similar aversive behavioral repertoire.
APPLIED ANIMAL BEHAVIOUR SCIENCE
(2021)
Article
Infectious Diseases
Cintia De Lorenzo, Matheus Viezzer Bianchi, Luiza Presser Ehlers, Andreia Vielmo, Paula Reis Pereira, Bruno Albuquerque de Almeida, Caroline Pinto de Andrade, Aline Girotto-Soares, Saulo Petinatti Pavarini, David Driemeier, Joao Fabio Soares, Luciana Sonne
Summary: This study investigated rangeliosis in canids and found differences in histopathological and molecular features as well as gene quantities between domestic dogs and wild foxes. The parasitic burden of rangeliosis in domestic dogs was significantly higher than in wild foxes, with similar results in certain organs indicating different roles in the development and transmission of rangeliosis between domestic dogs and wild foxes.
TICKS AND TICK-BORNE DISEASES
(2021)
Review
Virology
Tetsuaya Muto, Shinichiro Imaizumi, Koju Kamoi
Summary: Viruses, such as adenovirus, enterovirus, and herpes virus, account for 80% of acute conjunctivitis cases. Quick diagnosis, hand hygiene, and surface sanitization are crucial in controlling the spread of viral conjunctivitis. Adenovirus is the leading cause of viral conjunctivitis, and early detection is important to prevent a potential pandemic. Herpes simplex viral conjunctivitis diagnosis is essential to differentiate it from adenovirus conjunctivitis and determine appropriate treatment options. Although specific treatments may not be available, early diagnosis can help alleviate short-term symptoms and prevent long-term complications.
Article
Anatomy & Morphology
S. Viana-Pecanha, E. C. Souza, D. M. L. Guerra, F. C. S. Bernardes, R. B. J. Carvalho, P. de Souza Junior, M. Abidu-Figueiredo
Summary: The study described the origin, position, length, and main branches of the coeliac artery in Cerdocyon thous. The anatomical characteristics of the coeliac artery in this species are similar to those of other species in the Canidae family, possibly due to their phylogenetic proximity.
FOLIA MORPHOLOGICA
(2021)
Article
Parasitology
Nathalia Paula Scioscia, Yanina Paola Hecker, David Arranz-Solis, Julieta Pedrana, Facundo Nahuel Urtizbiria, Lucia Maria Campero, Leandro Olmos, Maria V. Scioli, Matias A. Dorsch, Franco Fiorani, Felipe Cheuquepan, Guillermo Maria Denegri, Gaston More, Dadin Prando Moore
Summary: This study evaluated Neospora caninum infection in the tissues of Argentine Pampas foxes and found a high prevalence of the parasite's DNA in brain tissue, confirming the fox's role as a wide distributed intermediate host.
PARASITOLOGY INTERNATIONAL
(2022)
Article
Multidisciplinary Sciences
Edward M. Kennedy, Agnieszka Denslow, Jacqueline Hewett, Lingxin Kong, Ana De Almeida, Jeffrey D. Bryant, Jennifer S. Lee, Judy Jacques, Sonia Feau, Melissa Hayes, Elizabeth L. McMichael, Daniel Wambua, Terry Farkaly, Amal A. Rahmeh, Lauren Herschelman, Danielle Douglas, Jacob Spinale, Sanmit Adhikari, Jessica Deterling, Matt Scott, Brian B. Haines, Mitchell H. Finer, Ted T. Ashburn, Christophe Queva, Lorena Lerner
Summary: The synthetic RNA virus platform provides a new approach for repeated intravenous administration of viral immunotherapy, by formulating synthetic RNA viruses within lipid nanoparticles, showing anti-tumor efficacy even in the presence of neutralizing antibodies against the virus.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Bruno de Souza Scramignon-Costa, Fernando Almeida-Silva, Bodo Wanke, Marcelo Weksler, Ricardo Moratelli, Antonio Carlos Francesconi do Valle, Rosely Maria Zancope-Oliveira, Rodrigo Almeida-Paes, Cecilia Bueno, Priscila Marques de Macedo
Summary: Wild animals infected with Paracoccidioides brasiliensis serve as important indicators of the presence of this fungal agent in the environment. Molecular detection of P. brasiliensis in road-killed animals has revealed new hosts for the fungus, enhancing our understanding of the ecology of this pathogen.
Article
Veterinary Sciences
Victor R. F. Ribeiro, Ariana F. Ramos, Angelica Alfonso, Alicia G. Hippolito, Heloisa C. Lima, Viviane M. Codognoto, Diogo C. S. Silva, Miriam H. Tsunemi, Moacir L. Neto, Alessandra Melchert, Priscylla T. C. G. Okamoto, Luiz H. A. Machado, Maria L. G. Lourenco
Summary: The aim of this study was to evaluate the cardiac condition of wild foxes through complimentary examinations. The results showed that compared to domestic canids, the cardiac size and blood flow velocity indices of the anesthetized foxes decreased, but the serum cardiac troponin levels remained normal. M-mode echocardiography proved to be a safe and effective method for assessing the cardiac function of wild canids.
VETERINARY RESEARCH COMMUNICATIONS
(2022)
Article
Environmental Sciences
Annalaura Carducci, Ileana Federigi, Elena Balestri, Claudio Lardicci, Alberto Castelli, Ferruccio Maltagliati, Hongrui Zhao, Virginia Menicagli, Rossella Valente, Davide De Battisti, Marco Verani
Summary: This study investigated the presence of viruses in seawater, sand, and beach-stranded material, and found that 13.6% of the environmental samples contained viral genome, but they were not infectious. Norovirus and SARS-CoV-2 were not detected. The study suggests that HAdV can be used as an indicator pathogen for beach monitoring and risk assessment.
MARINE POLLUTION BULLETIN
(2022)
Article
Microbiology
Caroline Giuseppa Spera, Fernanda Louise Pereira Lavorente, Elis Lorenzetti, Gustavo de Calasans Marques, Luana de Almeida Freitas, Maira Bonamin Martins, Carlos Roberto Teixeira, Amauri Alcindo Alfieri, Alice Fernandes Alfieri
Summary: The study found that a wild crab-eating fox pup in Brazil died from infection with canine parvovirus-2b (CPV-2b).
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2021)
Article
Virology
Marcilio Jorge Fumagalli, Luiza Antunes Castro-Jorge, William Marciel de Souza, Patrick Orestes de Azevedo, Alana Witt Hansen, Ricardo Tostes Gazzinelli, Benedito Antonio Lopes da Fonseca, Fernando Rosado Spilki, Luiz Tadeu Moraes Figueiredo
Summary: The emergence of the Delta variant of SARS-CoV-2 has raised concerns about vaccine effectiveness. However, this study found that the Delta variant is still neutralized effectively by antibodies produced from CoronaVac or ChAdOx1 vaccination, indicating the importance of vaccination even for individuals previously infected by the Gamma variant.
Article
Public, Environmental & Occupational Health
Mariana Soares da Silva, Juliana Schons Gularte, Meriane Demoliner, Alana Witt Hansen, Fagner Henrique Heldt, Micheli Filippi, Cristiane Borba Luckmann, Vyctoria Malayhka de Abreu Goes Pereira, Rodrigo de Almeida Vaucher, Victor dos Santos Barboza, Janice Luehring Giongo, Raquel Borba Rosa, Evelise Tarouco da Rocha, Bruno Kilpp Goulart, Fernanda dos Santos Fernandes, Juliana Maciel Pinto, Leandro Pergher Bolzan, Marta Maria Medeiros Frescura Duarte, Matheus Nunes Weber, Paula Rodrigues de Almeida, Juliane Deise Fleck, Fernando Rosado Spilki
Summary: A potentially new variant derived from the B.1.1.28 lineage was detected in travelers crossing the Brazil-Argentina border, representing an important peak of B.1.1.28 in RS state with a decreased frequency of the Gamma variant during the same period.
TRAVEL MEDICINE AND INFECTIOUS DISEASE
(2022)
Article
Virology
Andressa Taiz Hoffmann, Mariana Soares da Silva, Juliana Schons Gularte, Alessandro Comaru Pasqualotto, Jose Luiz Proenca Modena, Alana Witt Hansen, Claudio Marcel Berdun Stadnik, Teresa Cristina Teixeira Sukienik, Meriane Demoliner, Fagner Henrique Heldt, Micheli Filippi, Vyctoria Malayhka de Abreu Goes Pereira, Cristiani Gomes de Marques, Ionara Ines Kohler, Daniela Muller De Quevedo, Fernando Rosado Spilki
Summary: In this molecular epidemiology study, the occurrence and transmission dynamics of SARS-CoV-2 in outbreaks and local transmission chains were analyzed in a large tertiary teaching hospital in southern Brazil. Different lineages and sublineages were observed among patients in the same room. Most patients showed clinical manifestations, suspicions, and diagnostic confirmation within 7-14 days or >14 days after hospital admission. The average duration of contact between patients and confirmed cases of COVID-19 in the same environment was 6.28 days. The study site experienced a complete replacement of variant of concern (VOC) Gamma by VOC Delta from August 2021. Although nosocomial infections were indicated by epidemiological analysis, genomic sequencing confirmed different origins for most hospital outbreaks. These findings emphasize the importance of integrating epidemiological and genomic data to identify potential routes of viral entry and dissemination.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Micheli Filippi, Mariene Ribeiro Amorim, Mariana Soares da Silva, Juliana Schons Gularte, Meriane Demoliner, Viviane Girardi, Vyctoria Malayhka de Abreu Goes Pereira, Alana Witt Hansen, Juliane Deise. Fleck, Julia Frohlich, Fernanda de-Paris, Grazielle Motta Rodrigues, Janaina Aparecida Risczik Arruda Correa, Elissandra Machado Arlindo De Mattos, Rodrigo Minuto Paiva, Caroline Deutschendorf, Frederico Soares Falcetta, Jose Luiz Proenca Modena, Fernando Rosado Spilki
Summary: In this study, a case of a child with severe combined immunodeficiency and prolonged severe acute respiratory syndrome coronavirus 2 infection was reported. The amino acid changes present in naso-oropharyngeal swab samples collected between August and December 2021 were analyzed to examine the emergence of new viral variants. Genomic surveillance using high throughput sequencing proved to be a useful tool in assessing possible evolution signals in long-term patients.
PEDIATRIC INFECTIOUS DISEASE JOURNAL
(2023)
Article
Microbiology
Viviane Girardi, Juliana Schons Gularte, Meriane Demoliner, Mariana Soares da Silva, Micheli Filippi, Vyctoria Malayhka de Abreu Goes Pereira, Alana Witt Hansen, Raquel Borba Rosa, Juliane Deise Fleck, Fernando Rosado Spilki
Summary: In this study, a case of infection by the BA.1.1 sublineage followed by reinfection with the BA.2 sublineage was described in an individual from Southern Brazil. The two episodes of COVID-19 were caused by divergent VOC Omicron sublineages. This case represents the shortest reinfection period reported so far.
BRAZILIAN JOURNAL OF MICROBIOLOGY
(2023)
Article
Virology
Vivaldo Gomes da Costa, Ana Julia Chaves Gomes, Cintia Bittar, Dayla Bott Geraldini, Pamela Joyce Previdelli da Conceicao, agata Silva Cabral, Tamara Carvalho, Joice Matos Biselli, Paola Jocelan Scarin Provazzi, Guilherme Rodrigues Fernandes Campos, Paulo Ricardo da Silva Sanches, Paulo Inacio Costa, Mauricio Lacerda Nogueira, Joao Pessoa Araujo Jr, Fernando Rosado Spilki, Marilia Freitas Calmon, Paula Rahal
Summary: In the non-COVID-19 era, non-SARS-CoV-2 respiratory viral infections, such as influenza virus and human respiratory syncytial virus, significantly contributed to the burden of infectious diseases. This study found that the prevalence of influenza virus and respiratory syncytial virus was higher in the SARS-CoV-2-negative group than in the SARS-CoV-2-positive group. Therefore, non-SARS-CoV-2 respiratory viral infections should not be neglected during the early phase of the COVID-19 pandemic.
Article
Infectious Diseases
Victor dos Santos Barboza, William Borges Domingues, Thobias Toniolo de Souza, Tiago Veiras Collares, Fabiana Kommling Seixas, Bruna Silveira Pacheco, Fernanda Severo Sabedra Sousa, Thais Larre Oliveira, Marcelo de Lima, Claudio Martin Pereira de Pereira, Fernando Rosado Spilki, Janice Luehring Giongo, Rodrigo de Almeida Vaucher
Summary: This study evaluated the performance of the reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting SARS-CoV-2 in healthcare workers and compared it with the real-time reverse-transcription quantitative PCR (RT-qPCR) method. The results showed that the RT-LAMP method was effective in diagnosing SARS-CoV-2 infection in healthcare workers. A total of 100 clinical specimens from symptomatic and asymptomatic healthcare workers in Pelotas, Brazil were tested, and the E gene and N gene primers detected SARS-CoV-2 in 96.7% of the tested healthcare workers.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2023)
Article
Fisheries
Derek B. de Amorim, Paula R. Ribeiro, Marcele B. Bandinelli, Joanna V. Z. Echenique, Matheus V. Bianchi, Paula R. Almeida, Fernando R. Spilki, Leticia F. Baumbach, Luciana Sonne
Summary: This study reports a rare case of B-cell small lymphocytic lymphoma (B-SLL) found in a South American sea lion. The main pathological features of this case included poor body condition, generalized lymphadenomegaly, severe and diffuse splenomegaly, and multiple nodules in the kidneys and small intestine. Histological examination showed that these organs were partially or totally replaced by neoplastic lymphocytes with varying sizes and shapes, and a low mitotic count. These cells were immunolabeled positive for CD79 alpha and CD20, and negative for CD3.
DISEASES OF AQUATIC ORGANISMS
(2023)
Article
Virology
Juliana Schons Gularte, Livia Sacchetto, Meriane Demoliner, Viviane Girardi, Mariana Soares da Silva, Micheli Filippi, Vyctoria Malayhka de Abreu Goes Pereira, Alana Witt Hansen, Luana Leticia da Silva, Juliane Deise Fleck, Paula Rodrigues de Almeida, Mauricio Lacerda Nogueira, Fernando Rosado Spilki
Summary: In this study, the genetic diversity of the dengue virus circulating in southern Brazil during an outbreak in 2022 was monitored and characterized through next-generation sequencing. The results provided important information for managing outbreaks and controlling viral dissemination in the region.
JOURNAL OF CLINICAL VIROLOGY
(2023)
Article
Biochemical Research Methods
Paulo Augusto Esteves, Odir Antonio Dellagostin, Tamir Calcagnotto da Silva, Fernando Rosado Spilki, Alessandra D. Avila Silva, Eber Acacio Stodutto Oliveira, Ana Claudia Franco, Silvia Hubner, Claudio Chiminazzo, Claudio Wageck Canal, Fabricio Souza Campos, Paulo Michel Roehe
Summary: To avoid crossreactivity in seroprevalence studies, a new ELISA method (ELISA-gC1) was developed to detect antibodies to BoAHV1. The test showed 100% sensitivity and high specificity, allowing for the identification of BoAHV1-positive cattle without crossreactivity with BoAHV5 antibodies. This ELISA method is valuable for determining the prevalence of BoAHV1 and supporting BoAHV5 epidemiological studies.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Virology
Ueric Jose Borges de Souza, Ygor da Silva Miranda Macedo, Raissa Nunes dos Santos, Franciano Dias Pereira Cardoso, Jucimaria Dantas Galvao, Evgeni Evgeniev Gabev, Ana Claudia Franco, Paulo Michel Roehe, Fernando Rosado Spilki, Fabricio Souza Campos
Summary: The state of Tocantins in Brazil has experienced a significant number of cases of arboviral disease, particularly Dengue virus. Full genome sequencing of viral genomes recovered from patients revealed the circulation of different genotypes of Dengue virus in the state. Younger age groups were mostly affected, and there were no significant differences in gender distribution. This study highlights the importance of major population centers in Brazil in virus dispersion.
Article
Parasitology
Tatiana Schaffer Gregianini, Richard Steiner Salvato, Regina Bones Barcellos, Fernanda Marques Godinho, Amanda Pellenz Ruivo, Viviane Horn de Melo, Julio Augusto Schroder, Fernanda Leticia Martiny, Erica Bortoli Mollmann, Catia Favreto, Ludmila Fiorenzano Baethgen, Vithoria Pompermaier Ferreira, Livia Eidt de Lima, Claudia Fasolo Piazza, Tais Raquel Marcon Machado, Irina Marieta Becker, Raquel Rocha Ramos, Guilherme Carey Froelich, Alana Fraga Rossetti, Lucas da Cunha Almeida, Tahiana Machado Antunes Rodrigues, Isabella Tabelli Braganca, Aline Alves Scarpellini Campos, Veronica Bau Manzoni, Lais Ceschini Machado, Luisa Maria Inacio da Silva, Andre Luiz Sa de Oliveira, Marcelo Henrique Santos Paiva, Zenaida Marion Alves Nunes, Paula Rodrigues de Almeida, Meriane Demoliner, Juliana Schons Gularte, Mariana Soares da Silva, Micheli Filippi, Vyctoria Malayhka de Abreu Goes Pereira, Fernando Rosado Spilki, Ana Beatriz Gorini da Veiga, Gabriel Luz Wallau
Summary: Chikungunya is a mosquito-borne virus that has caused large outbreaks in the Americas since 2014. In Brazil, different genotypes have been detected and led to outbreaks in several states. In the southernmost state of Brazil, Rio Grande do Sul, the CHIKF burden has been increasing due to introductions and transmission of distinct lineages of the ECSA genotype.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2023)
Article
Infectious Diseases
Meriane Demoliner, Micheli Filippi, Juliana Schons Gularte, Mariana Soares da Silva, Paula Rodrigues de Almeida, Vyctoria Malayhka de Abreu Goes Pereira, Fagner Henrique Heldt, Fernando Rosado Spilki
Summary: New viruses of the Picornavirales order, including the human stool-associated RNA virus (husavirus [HuV]), have been discovered through high-throughput sequencing. However, there is still much unknown about HuV, such as its cellular host and evolutionary history. In this study, a HuV genome with 8,846 bp was identified for the first time in Southern Brazil, showing significant differences from Brazilian strains but high similarity with genomes from China, Vietnam, Venezuela, and the Netherlands. Further research and sequencing are necessary to improve our understanding of HuV and its unknown origin within the Picornavirales order.
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2023)
Article
Virology
Talita B. Machado, Agnello C. R. Picorelli, Bruna L. de Azevedo, Isabella L. M. de Aquino, Victoria F. Queiroz, Rodrigo A. L. Rodrigues, Joao Pessoa Araujo Jr, Leila S. Ullmann, Thiago M. dos Santos, Rafael E. Marques, Samuel L. Guimaraes, Ana Claudia S. P. Andrade, Juliana S. Gularte, Meriane Demoliner, Micheli Filippi, Vyctoria M. A. G. Pereira, Fernando R. Spilki, Mart Krupovic, Frank O. Aylward, Luiz-Eduardo Del-Bem, Jonatas S. Abrahao
Summary: Giant viruses with their gigantic genomes have sparked great interest, but the process of how these genomes evolved remains unclear. This study discovered a cedratvirus with the largest genome ever described, and identified gene duplication as a key mechanism driving genome expansion in giant viruses.
JOURNAL OF VIROLOGY
(2023)