Article
Environmental Sciences
Yao Chen, Ying Zhang, Liang Zhang, Shujun Zhang, Yongzhen Peng
Summary: Partial nitrification and partial denitrification combined with anammox is a promising process for sewage treatment, which was successfully achieved in this study by adding sludge fermentation products in the anoxic zone to facilitate nitrite accumulation and improve denitrification efficiency. The system achieved a high removal rate of total nitrogen, with enriched incomplete denitrifiers and ammonia oxidizing bacteria contributing to the high nitrite accumulation.
Article
Engineering, Environmental
Jianwei Li, Ruitao Gao, Qiyu Liu, Qiong Zhang, Xiyao Li, Liang Zhang, Junfei Qiao, Yongzhen Peng
Summary: The main challenge in municipal wastewater treatment using partial-denitrification/anammox (PD/A) in the anoxic zone is the effective enrichment of anaerobic ammonium oxidation (anammox) bacteria. This long-term study achieved a total inorganic nitrogen concentration of 11.2 ± 3.5 mg N/L in the effluent of a pure-biofilm anoxic/oxic reactor using real municipal wastewater, resulting in a removal efficiency of 77.4 ± 7.2%. The anammox contribution to total nitrogen removal was over 55%, and Candidatus Brocadia was the only anammox genus detected. The study highlights the robust enrichment of anammox bacteria using a pure-biofilm system, providing insight into the application of mainstream anammox in municipal wastewater treatment.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Yunlong Su, Rui Du, Jiao Wang, Xiyao Li, Qiong Zhang, Xiaofei Xue, Yongzhen Peng
Summary: In this study, anammox bacteria were efficiently enriched and retained in a suspended sludge system for mainstream wastewater treatment. The system provided favorable conditions for the growth of anammox bacteria, including low nitrogen loading rate, low ammonia and nitrate concentrations, and anoxic environment. This research demonstrated the feasibility and potential of applying anammox technology without specific sludge seeding in mainstream wastewater treatment.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Boran Wu, Xiuzhong Wang, Chengxian Wang, Bin Lu, Jing Yi, Xiaohu Dai, Xiaoli Chai
Summary: A novel high-concentration powder bio-carrier (HPB) process was developed for the high-load treatment of low-strength municipal wastewater with low C/N ratio, which facilitated rapid sludge micro-granulation and enhanced nitrogen removal efficiency. The process achieved higher volumetric load and optimized microbial structure, leading to promising results for the highly efficient treatment of low-strength municipal wastewater.
Article
Agricultural Engineering
Luyuan Zhang, Qiong Zhang, Xiyao Li, Tong Jia, Shuying Wang, Yongzhen Peng
Summary: This study established a novel combined process of anaerobic ammonia oxidation using a sequencing batch reactor and up-flow sludge beds, achieving efficient nitrogen removal from municipal wastewater.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Bin Ma, Yanbing Liang, Yujian Zhang, Yan Wei
Summary: A partial-nitrification/anammox and endogenous partial-denitrification/anammox (PN/A-EPD/A) process was developed in a sequential batch biofilm reactor (SBBR) to achieve advanced nitrogen removal from low-carbon wastewater. The effluent total nitrogen (TN) reached 3.29 mg/L when the influent COD/TN and the TN were 2.86 and 59.59 mg/L, respectively. The stable PN/A-EPD/A process was achieved through four strategies, including treating the inoculated sludge with free nitrous acid, inoculating anammox biofilm, discharging excess activated sludge and residual ammonium at the end of oxic stage. The 16S rRNA high-throughput sequencing results showed the coexistence of anammox bacteria, ammonia oxidizing bacteria, nitrite oxidizing bacteria, denitrifying glycogen accumulating organisms (DGAOs) and denitrifying phosphorus accumulating organisms (DPAOs) in the biofilms, with higher abundance of anammox bacteria in the inner layer and higher abundance of DGAOs and DPAOs in the outer layer.
BIORESOURCE TECHNOLOGY
(2023)
Article
Engineering, Environmental
Qiyu Liu, Jianwei Li, Yang Zhao, Xiyao Li, Qiong Zhang, Jun Sui, Chuanxin Wang, Yongzhen Peng
Summary: The reliability of anammox-technical application in municipal wastewater treatment depends on the enrichment of anammox bacteria. The study investigated the interaction mechanism between suspended sludge and anoxic biofilm, and found that the presence of suspended sludge significantly decreased the nitrogen removal efficiency of anammox. The spatial distribution of anammox bacteria within the biofilm changed with the presence or absence of suspended sludge.
Article
Agricultural Engineering
Xiuhong Liu, Runyu Liu, Qing Yang, Bin Cui, Wenjun Wu, Xiaoyin Zhao, Yaxin Wang
Summary: Partial denitrification was successfully achieved in a municipal wastewater treatment plant, providing stable nitrite for anammox while controlling nitrite accumulation, with dominant microbial communities identified as Candidatus Competibacter and Thaurea. The study also found coexistence of various denitrifying organisms, contributing to a high COD specific removal rate during partial denitrification.
BIORESOURCE TECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Evan Ronan, Hussain Aqeel, Gideon M. Wolfaardt, Steven N. Liss
Summary: High strength ammonia wastewater treatment requires advanced technologies such as biofilm and aerobic granular sludge to improve system performance. Additionally, new methods like the Anammox process and the use of photosynthetic bacteria for ammonia recovery offer more cost-effective ways to remove reactive nitrogen.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Hong Chen, Zhi Tu, Sha Wu, Guanlong Yu, Chunyan Du, Hong Wang, Enzhe Yang, Lu Zhou, Bin Deng, Dongbo Wang, Hailong Li
Summary: The novel process of partial denitrification (PD) coupled with anaerobic ammonium oxidation (AMX) shows great potential for nitrogen removal in municipal wastewater treatment. Research focuses on process mechanisms, microbial community characteristics, key operational parameters, stable operation, and control methods. Challenges lie in engineering and economic feasibility, full mainstream AMX application, and further study of PD-AMX mechanism optimization for better engineering practice.
Article
Engineering, Environmental
Yang Zhao, Jianwei Li, Qiyu Liu, Zhao Qi, Xiyao Li, Qiong Zhang, Jun Sui, Chuanxin Wang, Yongzhen Peng
Summary: This study investigated the in situ start-up of a partial-denitrification/anammox (PD/A) process in a traditional A(2)/O process and successfully started up the PD/A system within 60 days. The nitrogen removal efficiency of the system increased by 16.9% and the in situ anammox activity reached 1.0 mg NH4+-N/L/h, contributing 37.9% to total nitrogen removal. The study also found that the addition of in situ AnAOB showed a better coordination between denitrifiers and AnAOB in the system.
Article
Agricultural Engineering
Ruitao Gao, Yongzhen Peng, Jianwei Li, Qiong Zhang, Xiyao Li, Liyan Deng, Wenyu Li, Chengkun Kao
Summary: The study confirmed that the PN/A-PD/A process with partial anammox, spatially coupled with partial nitrification and partial denitrification via oxic/anoxic distribution with step feed mode, is an alternative for application of mainstream anammox in municipal wastewater treatment. The process achieved stable nitrogen loss, improved anammox activity, and increased abundance on the biocarriers.
BIORESOURCE TECHNOLOGY
(2021)
Article
Agricultural Engineering
Huihui Cui, Liang Zhang, Qiong Zhang, Xiyao Li, Yu Huang, Yongzhen Peng
Summary: The study developed an innovative partial nitrification-anammox and endogenous partial denitrificationanammox process for efficient treatment of real municipal wastewater with a C/N ratio below 3.2. The nitrogen removal mechanism analysis revealed the importance of anammox bacteria and endogenous denitrifying bacteria in biofilms and suspended sludge for improving nitrogen removal efficiency. Overall, the combined PN/A and EPD/A process proved to be a reliable and efficient alternative for mainstream anammox processes.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Xinjie Gao, Qiong Zhang, Zeming An, Baishou Shao, Yongzhen Peng
Summary: Anaerobic ammonium oxidation (anammox) combined with partial nitrification (PN) can achieve energy self-sufficiency in wastewater treatment. The PNA process is beneficial for advanced and efficient nitrogen removal in carbon-limited wastewater treatment.
RESOURCES CONSERVATION AND RECYCLING
(2023)
Article
Agricultural Engineering
Wenkang Lu, Yanli Zhang, Qingqing Wang, Yan Wei, Yinan Bu, Bin Ma
Summary: A novel partial denitrification/anammox-nitrifying (PDA-N) biofilter process was developed for treating municipal wastewater, achieving a total inorganic nitrogen removal efficiency of 81%. Anammox played a primary role, achieving approximately 98% of the total inorganic nitrogen removal, and the process showed potential for economizing organic carbon demand and oxygen consumption compared to traditional methods.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Yandong Yang, Yiming Jiang, Yanan Long, Jiarui Xu, Changqing Liu, Liang Zhang, Yongzhen Peng
Summary: This study investigates the impact of residual ammonium on the stability of mainstream partial nitritation/anammox (PN/A) systems and reveals that low ammonium conditions lead to a decline in anammox activity and increased nitrite oxidation rate. The study provides insights for the optimized design of mainstream PN/A systems in engineering applications.
JOURNAL OF ENVIRONMENTAL SCIENCES
(2023)
Review
Agricultural Engineering
Liang Zhang, Ling Jiang, Jiangtao Zhang, Jialin Li, Yongzhen Peng
Summary: Anaerobic ammonium oxidation (anammox) has great potential for nitrogen removal from mainstream wastewater. However, there are limitations to its direct application. This review summarizes the technical development of partial anammox and the mechanisms of achieving partial mainstream anammox through aeration and organic control.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agricultural Engineering
Zhichao Hao, Liang Zhang, Qiong Zhang, Yongzhen Peng, Liangliang Shi, Yanan Li
Summary: This study established a single-stage simultaneous alkaline fermentation coupled with partial denitrification system, achieving reduction of primary sludge and removal of nitrate. It also laid a solid foundation for anammox process with high nitrite production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Engineering, Environmental
Huihui Cui, Liang Zhang, Qiong Zhang, Xiyao Li, Yongzhen Peng
Summary: This study found that comammox bacteria, which were previously overlooked, are the most important ammonia-oxidizing microbes in Anammox systems, and play a significant role in nitrogen removal.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Xinjie Gao, Liang Zhang, Yongzhen Peng, Jing Ding, Zeming An
Summary: The study found that stable nitrogen removal can be achieved in the anaerobic/aerobic/anoxic process by directly integrating stable anammox. The anammox activity compensates for the effects of low temperatures and low C/N ratios on nitrification and denitrification, leading to a stable nitrogen removal efficiency.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Green & Sustainable Science & Technology
Xiaofei Gong, Liang Zhang, Xiyao Li, Qiong Zhang, Taotao Shang, Jingwen Guo, Chuanxin Wang, Shuying Wang, Yongzhen Peng
Summary: This study proposed a novel Integrated Anammox, Fermentation and Denitrification (IAFD) process which achieved a high efficiency of nitrogen removal from wastewater. The process showed environmental and economical advantages with no external carbon requirement, reduced aeration, and sludge reduction.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Environmental Sciences
Tipei Jia, Liang Zhang, Shihao Sun, Qi Zhao, Yongzhen Peng
Summary: In this study, acidophilic mixotrophic sulfur-oxidizing bacteria (SOB) were enriched under extremely acidic conditions to enhance bio-desulfurization performance. The addition of organics improved the H2S removal efficiency. Maintaining an appropriate biofilm mass is crucial for optimal desulfurization performance. The findings of this study offer a novel strategy for enhancing H2S removal.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Environmental Sciences
Tipei Jia, Liang Zhang, Xiyao Li, Qi Zhao, Yongzhen Peng, Jun Sui, Chuanxin Wang
Summary: This study investigates the impact of seasonal temperature variations on the performance of a biotrickling filter system in removing hydrogen sulfide from air. It is found that the system achieves the highest removal efficiency at temperatures of 10-12 degrees Celsius. The accelerated growth of biofilm, enhanced biofilm adhesion capacity, and sulfur accumulation contribute to heat retention in cold conditions.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Shenhua Yang, Haibin Chang, Yongzhen Peng, Shujun Zhang, Xiaoyu Han, Shihao Sun, Jinjin Liu, Liang Zhang
Summary: Adding fermented sludge products to SBR can enhance nutrient removal and reduce sewage treatment costs.
WATER ENVIRONMENT RESEARCH
(2023)
Article
Engineering, Environmental
Hanbin Wang, Liang Zhang, Qiongpeng Dan, Yingxin Zhang, Shuying Wang, Qiong Zhang, Xiyao Li, Chuanxin Wang, Yongzhen Peng
Summary: Integrating endogenous denitrification (ED) into partial nitrification-anammox (PNA) systems using organic matter in municipal wastewater effectively improves nitrogen removal efficiency. A novel strategy was adopted to inhibit phosphorus-accumulating organisms (PAOs) by inducing phosphorus release and exclusion, optimizing organics allocation between PAOs and glycogen-accumulating organisms (GAOs). This study achieved an NRE of 97.5 +/- 2.2% and effluent total inorganic nitrogen (TIN) of less than 2.0 mg/L using enhanced ED-synergized anammox for real municipal wastewater treatment.
Article
Environmental Sciences
Bingxin Li, Ruochen Wang, Weiwei Zuo, Yi Peng, Dong An, Liang Zhang, Zheng Ge
Summary: A lab-scale ceramic membrane bioreactor (MBR) with an active membrane-fouling control system was developed for the partial nitrification (PN) process. The in situ membrane cleaning method was used for uninterrupted removal of contaminants on the membrane surface. The device increased critical flux, reduced gel layer resistance (R-g), and internal resistance (R-i) of the flat-sheet ceramic membrane by inhibiting cake layer formation. In long-term experiments, nitrite oxidizing bacteria (NOB) were successfully suppressed, achieving high nitrite accumulation rate (NAR) of up to 90.09%. The maintained balance between effluent NO2--N/NH4+-N ratios would promote the proliferation of Anammox bacteria and the autotrophic nitrogen removal (ANR) process. Moreover, in situ cleaning significantly reduced energy input from aeration and ensured stable PN performance by avoiding over aeration.
Article
Engineering, Environmental
Yandong Yang, Yongzhen Peng, Jun Cheng, Shujun Zhang, Changqing Liu, Liang Zhang
Summary: A novel two-stage aerobic granular sludge (AGS) system was developed for municipal wastewater treatment, achieving high removal efficiencies of phosphorus (91%) and nitrogen (81%) with a low influent carbon/nitrogen ratio. The high nutrient removal was attributed to microbial segregation in the two SBRs. The first reactor promoted enhanced biological phosphorus removal (EBPR) with the high abundance of polyphosphate-accumulating organisms (Candidatus Accumulibacter 15.3%), while the second reactor removed residual ammonium via partial nitritation/ anammox (PN/A). The system also facilitated sludge maintenance and reduced settling time, providing opportunities for resource and bioenergy recovery.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Agricultural Engineering
Xiyue Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: Intermittent aeration is an innovative approach to enhance nitrogen removal in low carbon-to-nitrogen ratio municipal sewage, providing an efficient strategy for the continuous plug-flow AOA process.
BIORESOURCE TECHNOLOGY
(2024)
Article
Agricultural Engineering
Zhaoyang Li, Liang Zhang, Yongzhen Peng, Jialin Li
Summary: This study investigated the relationship between the performance and ecological features of a one-stage partial nitritation and anammox system disturbed by oxygen. The disturbance resulted in an irreversible deterioration of nitrogen removal rate and reduced richness and evenness of the microbial community. Additionally, the community structure changed and 234 operational taxonomic units were depleted. The study highlights the importance of ecological features in diagnosing, predicting, and optimizing the performance of partial nitritation and anammox systems.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Xiaofei Gong, Feng Hou, Hongtao Pang, Yuanyuan Guo, Qiong Zhang, Xiyao Li, Liang Zhang, Yongzhen Peng
Summary: This study achieved stable and efficient nitrogen removal through a zero-external carbon Partial Nitritation/Anammox combined with in-situ sludge Fermentation-Denitrification process under decreased temperatures. The findings show the resilience of anammox to lower temperatures and highlight the importance of sludge reduction and organic recovery for enhanced denitrification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2023)
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.
