Review
Food Science & Technology
Bruno Nicolau Paulino, Adones Sales, Lorena Felipe, Glaucia Maria Pastore, Gustavo Molina, Juliano Lemos Bicas
Summary: Aroma is a crucial attribute of food directly impacting consumer acceptance. In recent decades, interest in biotechnologically produced aroma compounds has grown as a sustainable way to provide natural additives for the food industry. Recent advances in microbial and enzymatic production of aroma compounds demonstrate their high potential for commercial applications.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Xuan Liu, Wei Shi, Long Xu, Bo Yang, Sentai Liao, Dongming Lan, Weifei Wang, Yonghua Wang
Summary: This study successfully prepared alpha-linolenic acid-enriched diacylglycerols (ALA-DAGs) using a two-step enzymatic method with silkworm pupae oils as substrates. The method involved hydrolysis of the oil and enrichment of ALA, resulting in a high content of ALA in the final product. Overall, this study provides a promising way for the preparation of ALA-DAGs.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Energy & Fuels
Polinati Satya Sagar, Satish Kommoji, Iyyappan Jayaraj, Deepanraj Balakrishnan, Feroz Shaik, Mahendra Vucha, Lalitha Gnanasekaran
Summary: This study investigated the optimization of thermal-diluted acid pretreatment on native grass biomass for simple sugar recovery, followed by lipid bioproduction using Yarrowia lipolytica. The maximum total reducing sugar (34.26 +/- 1.55 g/L) in the native grass hydrolysate was obtained at 163.8 degrees C, 1.57% (v/v) sulfuric acid concentration for 21 min. Detoxified native grass hydrolysate yielded 11.64 +/- 0.84 g/L of lipid with 65.52% (w/w) lipid content after 96 h using Y. lipolytica. These results highlight the efficiency of optimizing thermal-diluted acid pretreatment on native grass biomass for lipid production using Y. lipolytica.
Article
Agricultural Engineering
Yanzhe Shang, Ping Zhang, Wenping Wei, Jin Li, Bang-Ce Ye
Summary: By optimizing carbon metabolism, increasing gene copies, and blocking degradation, a high yield of polydatin was successfully produced in Y. lipolytica, demonstrating its great potential for glycoside synthesis.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ana S. Pereira, Isabel Belo, Marlene Lopes
Summary: Microbial lipids from Yarrowia lipolytica can be used as feedstocks for the biodiesel industry. Two-stage batch cultures are an effective strategy to improve the production of lipids. Volatile fatty acids obtained from anaerobic fermentation of organic wastes can reduce the production costs. The addition of VFAs mixture in the lipogenic phase can enhance microbial lipids production and maintain stability, with the main component being oleic acid, meeting the EU biodiesel standard.
APPLIED SCIENCES-BASEL
(2022)
Article
Agricultural Engineering
Jinpeng Wang, Xiao Yu, Kaifeng Wang, Lu Lin, Hu-Hu Liu, Rodrigo Ledesma-Amaro, Xiao-Jun Ji
Summary: This study explored the optimal biosynthetic pathways for customized production of co6-PUFAs in Y. lipolytica and effectively increased the proportions of co6-PUFAs in total fatty acids through various strategies. The customized strains synthesized GLA, DGLA, and ARA with high titers, providing valuable insights into the production of functional co6-PUFAs.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jin-Zheng Wang, Cheng-Kun Wu, Cheng-Hai Yan, Huan Chen, Shuai You, Sheng Sheng, Fu-An Wu, Jun Wang
Summary: A microfluidic reactor with smart hydrogel immobilized lipase was used to prepare a nutritional supplement using silkworm pupae oil (SPO) as a feedstock, successfully improving the nutritional function by reducing palmitic acid content and increasing triglyceride content. The in vitro digestion model confirmed the enhanced fatty acid release rate of the modified SPO, indicating an overall improvement in nutritional function.
Article
Agriculture, Dairy & Animal Science
K. M. Rashmi, M. Chandrasekharaiah, N. M. Soren, K. S. Prasad, C. G. David, Y. Thirupathaiah, V Shivaprasad
Summary: This study evaluated the effect of different levels of defatted silkworm pupae meal (DSWP) on rumen fermentation, microbial protein synthesis and nutrient utilisation in cattle. The results showed that DSWP can replace soybean meal as a feed ingredient for cattle without affecting rumen fermentation and nutrient utilisation.
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2022)
Article
Energy & Fuels
Zong-Nan Li, Wen-Jing Li, Jin-Zheng Wang, Shuai You, Jun Wang, Fu-An Wu
Summary: The study found that defatted silkworm pupae hydrolysate as a nitrogen source can enhance the production of polysaccharides and flavonoids by Phellinus baumii. The concentration of extracellular polysaccharides and flavonoids produced using this alternative nitrogen source was significantly higher than other nitrogen source treatment groups, indicating its great economic value.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Biochemistry & Molecular Biology
Cheng-Hai Yan, Xiao-Meng Xun, Jiao Wang, Jin-Zheng Wang, Shuai You, Fu-An Wu, Jun Wang
Summary: alpha-Linolenic acid (ALA) has a regulatory effect on cardiovascular diseases, and silkworm pupae oil (SPO) can be a more easily accepted alternative supplement due to its higher release of free fatty acids (FFAs), smaller emulsion droplet size, weaker surface charge, and quicker absorption in the human body compared to linseed oil (LINO). Overall, SPO can be used as a new alternative for ALA supplements based on its superior digestive properties.
Article
Microbiology
Caleb Walker, Seunghyun Ryu, Sergio Garcia, David Dooley, Brian Mendoza, Cong T. Trinh
Summary: This study identified genotypes that contribute to high IL tolerance in Y. lipolytica and shed light on their potential mechanisms using RNA-Seq data and the Gene Coexpression Connectivity method.
Article
Agriculture, Multidisciplinary
Lei Zheng, XingYi Zhu, Jialu Wang, Weike Su
Summary: The study developed mechanochemical-assisted extraction (MCAE) and mechanochemical-assisted enzyme catalysis (MCAEC) methods for extracting and enzymatic hydrolysis of calcium phytate from defatted rice bran, respectively. The optimized MCAE conditions resulted in a calcium phytate yield of 97.0 mg/g, while the optimized MCAEC conditions achieved a hydrolysis rate of 39.7% for calcium phytate. Compared to traditional methods, MCAE and MCAEC demonstrated higher yields and hydrolysis rates, respectively.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Green & Sustainable Science & Technology
Jing Cao, Jian Yang, Yishuo Yang, Zhaomei Wang
Summary: The study employed a two-step sequential approach with UV-catalyzed alkaline hydrogen peroxide and ionic liquid treatment to achieve high saccharification in sisal waste, resulting in increased lignin removal, cellulose content, and reduced crystallinity index.
Article
Energy & Fuels
Mengxuan Zhang, Yunyun Liu, Wen Wang, Qiong Wang, Cuiyi Liang, Yunzi Hu, Wei Qi
Summary: This study improved the enzymatic saccharification efficiency of spent mushroom substrate (SMS) by treating it with mechanical pulverization and ball milling. Smaller initial particle size of SMS resulted in higher enzymatic hydrolysis efficiency, and proper ball milling time was better for improving the enzymatic hydrolysis of SMS.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Environmental Sciences
Zi-Qian Zha, Shuai You, Yang-Hao Hu, Fang Zhang, Yi-Wen Chen, Jun Wang
Summary: N-glycosylation can alter the properties of enzymes, such as increasing thermostability and catalytic efficiency. This study focused on the modification and utilization of GH16 family glucanases, showing that N-glycosylation can enhance their stability and efficiency.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Yu-Shi Shen, Xing Zhou, Shuai You, Lu-Chan Gong, Jun Wang, Tao Chen, Wei-Guo Zhao, Xiao-Hui Yao, Dong-Yang Zhang
Summary: Inspired by the structure of natural wood, a cellulose-based bionic microfluidic biocatalytic reactor was developed for the continuous fluid catalytic conversion of polydatin to resveratrol. The reactor, with vertically aligned channels and a porous inner wall, showed high catalytic activity (98.69% treatment efficiency) and excellent stability (maintain a conversion rate of more than 90% within 15 days) due to the unidirectionally aligned channels and porous inner wall. The reactor has great potential for simplifying industrial production and has broad application prospects for catalytic reactions.
Article
Agricultural Engineering
Shuai You, Lin-Lin Zhu, Shu-Meng Zhang, Richard-Ansah Herman, Yang Li, Li-Tian Geng, Huan Chen, Fu-An Wu, Jun Wang
Summary: In this study, a mutant of GH78 family RhaB1, RhaB1-ΔN, was obtained by deleting the domain N, which exhibited improved thermostability and catalytic activity. Using RhaB1-ΔN as a whole-cell catalyst, the conversion of isoquercitrin reached 96.6% within 1.5 hours, 8.5 hours less than that for free RhaB1. The combined strategies of enzyme modification and whole-cell catalyst enabled more recyclable and efficient isoquercitrin production.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Review
Polymer Science
Richard Ansah Herman, Xuan Zhu, Ellen Ayepa, Shuai You, Jun Wang
Summary: The use of biochemical enzymes in polymer science is crucial for maintaining their catalytic activity. The one-step synthesis of polymers with highly sequence-controlled enzymes is a strategy to enhance their catalytic activity and stability in sustainable materials. Various techniques such as enzyme-catalyzed chain growth polymerization and protein-polymer complexation can be used to develop formulations that ensure enzyme stability during complex material processes. The green synthesis of enzyme-catalyzed materials has attracted significant interest due to economic and environmental advantages. This review analyzes strategies and material technologies used in multi-enzyme cascade platforms for engineering polymer materials, providing an update on the trends and gaps in one-step synthesis of materials using catalytic enzymes.
Article
Biotechnology & Applied Microbiology
Can-Yang Shi, Yu-Fan Zhan, Yang Liu, Zhan-Peng Zhang, Xiang-Yu Shen, Cheng-Kun Wu, Zhi-Yuan Bai, Zhi-Ang Zhang, Jun Wang
Summary: Xylooligosaccharides have gained attention as a valuable non-renewable resource due to resource shortage. This study synthesized XYL-polymer conjugates by grafting alkyl chain-containing monomers onto xylanase, improving stability and catalytic efficiency. XYL-pBMA showed the highest relative activity, retaining 67.61% after heat treatment, and had 1.3 times the catalytic efficiency of free xylanase. XYL-pBMA also retained over 60% residual activity after multiple reuses and efficiently hydrolyzed the mulberry branch into oligosaccharides.
BIOCHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Environmental
Jin-Zheng Wang, Cheng-Kun Wu, Xin-Ying Wang, Yuan-Zhi Xu, Richard Ansah Herman, Sheng Sheng, Shuai You, Jun Wang, Fu-An Wu
Summary: Edible insects are a valuable resource due to their rich oil and protein content, and their sustainable development is crucial in the face of increasing scarcity of resources. To address the challenges in extracting both oil and protein simultaneously from edible insects, a novel enzyme-assisted aqueous extraction technique, CNPs@Cu-Trypsin-assisted aqueous extraction, was proposed. This technique significantly improved hydrolysis activity and the formation of hydrophilic microenvironment, leading to a high oil extraction ratio of 93% for silkworm pupae. Moreover, the quality of the extracted oil and the biological activity of the protein hydrolysate were also improved. This strategy offers a green and sustainable approach to explore edible insect oil and protein without the use of organic solvents.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Chemistry, Applied
Yi-Tong Li, Ting Huang, Jin-Zheng Wang, Cheng-Hai Yan, Lu-Chan Gong, Fu-An Wu, Jun Wang
Summary: By utilizing direct hydrolysis of the rhamnose bond and whole-cell catalyzed conversion, combined with an aqueous two-phase system and semi-preparative HPLC separation and purification, high-purity C3G was rapidly prepared, providing a novel strategy for efficient biomanufacturing.
Article
Agriculture, Multidisciplinary
Yi-Jiangcheng Li, Tian-Le Hong, Hong-Chao Chen, Feng-Ming Gu, Zhi-Xiang Liu, Shuai You, Fu-An Wu, Sheng Sheng, Jun Wang
Summary: Meteorus pulchricornis is a parasitic wasp that preys on various lepidopteran pests. The use of broad-spectrum insecticides poses a serious threat to the olfactory recognition of nontarget insects like parasitoid wasps. In this study, the researchers discovered that the MpulOBP6 protein exhibited strong binding affinity to three insecticides, and the hydrophobic interaction was the primary driving force in the formation and stabilization of MpulOBP6-insecticide complexes. The findings have significant implications for understanding the impact of insecticide use on the olfactory recognition of nontarget insects in agricultural production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Engineering, Environmental
Richard A. A. Herman, Xuan Zhu, Ellen Ayepa, Wen-Xin Zhang, Michael Ackah, Shuai You, Jun Wang
Summary: The present study aimed to develop chitosan/collagen protease-based composite membranes loaded with enzyme nanoparticles and evaluate their biodegradability using a recombinantly produced aspartic protease. The results showed that the ENPs and the chitosan-collagen composite membranes were highly compatible. The ENPs loaded membranes exhibited improved antioxidant and microbial growth inhibitory activities, and the respective protease showed enhanced specificity to its substrates.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2023)
Article
Green & Sustainable Science & Technology
Zhi-Yuan Bai, Shuai You, Fang Zhang, Zhi-Wei Dong, Yi-Fan Zhao, Hong-Jian Wen, Jun Wang
Summary: The study modified xylanase through rational design and used it with cellulase to synergistically degrade mulberry branches pretreated with seawater. The mutant S21Y/N318W showed improved thermostability and specific activity. Under the condition of seawater immersion pre-treatment, the highest yield of fermentable sugar and degree of synergy were achieved. This study provides an efficient method of biomass saccharification for biofuel production.
Article
Energy & Fuels
Cheng-Kun Wu, Yuan-Zhi Xu, Zhi-Yuan Bai, Can-Yang Shi, Jin-Zheng Wang, Cheng-Hai Yan, Lu-Chan Gong, Shuai You, Jun Wang
Summary: A novel approach using sequential simulated moving bed (SSMB) chromatography column was developed to extract and purify tri-alpha-linolenin from silkworm pupae oil. The SSMB approach resulted in significantly increased purity and yield of tri-alpha-linolenin compared to conventional extraction methods, with a 26.7-fold increase in yield and a 2-fold reduction in solvent usage.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Chemistry, Multidisciplinary
Xiao-Meng Xun, Zhi-Ang Zhang, Zi-Xuan Yuan, Kamila Tuhong, Cheng-Hai Yan, Yu-Fan Zhan, Guo-Ping Kang, Qiong-Ying Wu, Jun Wang
Summary: Microcapsules containing bioactive peptides from silkworm pupae were successfully prepared using chitosan and caffeic acid-grafted chitosan (CA-g-Ch) as carriers and microfluidics as the encapsulation method. The optimized formulation of CA-g-Ch-sodium alginate microcapsules exhibited high encapsulation efficiency, improved thermal stability, and enhanced antioxidant properties. These microcapsules showed good acid resistance and controlled release in gastrointestinal digestion, making them promising environmentally friendly carriers for hydrophilic drug delivery.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Engineering, Environmental
Lei Zhang, Minglu Hao, Liang Yao, Cheng Xing, Qingmei Wen, Zhining Zhang, Jingmou Yu, Jun Wang, Dongming Xing, Tao Zheng, P. Chen
Summary: This study used silk sericin to encapsulate anthocyanins, improving their stability and bioactivity, and investigated the interaction between silk sericin and anthocyanins through experimental and computational methods. The hydrogel formulation of silk sericin showed higher encapsulation efficiency and stability under different conditions. The encapsulated anthocyanins exhibited enhanced bioactivity, particularly in scavenging reactive oxygen species. This study highlights the potential of using two bioactive components from the same source to enhance stability and regulate bioactivity in tissue engineering applications.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Food Science & Technology
Jun Zhuo, Jiajia Xuan, Yao Chen, Jie Tu, Huirong Mu, Jun Wang, Guanhui Liu
Summary: The strain JS35, isolated from silkworm intestines, showed the highest ability in producing gamma-aminobutyric acid (GABA) among the screened strains. The strain belonged to Pediococcus pentosaceus based on 16S rRNA gene sequencing. Fermentation by JS35 improved the flavor and content of mulberry leaf powder (MLP), while reducing the anti-nutritional factor phytic acid content, indicating its potential for further processing of MLP products.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Green & Sustainable Science & Technology
Shuai You, Wen-Xin Zhang, Yan Ge, Yu Lu, Richard Ansah Herman, Yi-Wen Chen, Sheng Zhang, Yang-Hao Hu, Zhi-Yuan Bai, Jun Wang
Summary: In this study, a dominant mutant H51K/Q118A derived from Gloeophyllum trabeum was obtained by directed evolution, which showed higher specific activity, catalytic efficiency, and thermostability compared to the wild type. The mutant also exhibited impressive pH resistance from pH 1.0-9.0. When combined with 'Seawater + Feton' pretreatment, the mutant achieved a lignin clearance rate of 62.5%, 86.6% higher than feton pretreatment. Synergistic hydrolysis with the mutant and cellulase increased the fermentable sugar yields from bagasse by 48.4%.